HRP20190634T1 - Životinje koje nisu ljudi, koje imaju humanizirani gen 47 iz razlikovnog grozda - Google Patents

Životinje koje nisu ljudi, koje imaju humanizirani gen 47 iz razlikovnog grozda Download PDF

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HRP20190634T1
HRP20190634T1 HRP20190634TT HRP20190634T HRP20190634T1 HR P20190634 T1 HRP20190634 T1 HR P20190634T1 HR P20190634T T HRP20190634T T HR P20190634TT HR P20190634 T HRP20190634 T HR P20190634T HR P20190634 T1 HRP20190634 T1 HR P20190634T1
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Croatia
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rodent
gene
endogenous
humanized
human
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HRP20190634TT
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Cagan Gurer
Ella Ioffe
Alexander MUJICA
Gavin Thurston
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Regeneron Pharmaceuticals, Inc.
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=54979926&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HRP20190634(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharmaceuticals, Inc. filed Critical Regeneron Pharmaceuticals, Inc.
Publication of HRP20190634T1 publication Critical patent/HRP20190634T1/hr

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    • A01K67/027—New or modified breeds of vertebrates
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    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
    • A61K49/0008—Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
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    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
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    • C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
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    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
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Claims (12)

1. Glodavac čiji genom sadrži humanizirani gen CD47, naznačen time što humanizirani gen CD47 sadrži egzon 1 iz endogenog glodavačkog gena CD47, egzone 2-7 iz ljudskog gena CD47, te egzone nizvodno od egzona 7 iz endogenog glodavačkog gena CD47, gdje je humanizirani gen CD47 operabilno spojen s promotorom endogenog glodavčkog CD47.
2. Glodavac u skladu s patentnim zahtjevom 1, naznačen time što glodavac dodatno sadrži gen SIRPα, koji kodira polipeptid SIRPα, koji sadrži izvanstaničnu domenu ljudskog polipeptida SIRPα i unutarstaničnu domenu endogenog glodavačkog polipeptida SIRPα, gdje polipeptid SIRPα međudjeluje s CD47, te gdje gen SIRPα može sadržavati egzone 1, 5, 6, 7 i 8 iz endogenog glodavačkog gena SIRPα i egzone 2-4 iz ljudskog gena SIRPα.
3. Glodavac u skladu s patentnim zahtjevom 1 ili patentnim zahtjevom 2, naznačen time što je glodavac štakor ili miš.
4. Izolirana glodavačka stanica ili tkivo čiji genom sadrži humanizirani gen CD47, naznačeni time što humanizirani gen CD47 sadrži egzon 1 iz endogenog glodavačkog gena CD47, egzone 2-7 iz ljudskog gena CD47, te egzone nizvodno od egzona 7 iz endogenog glodavačkog gena CD47, gdje je humanizirani gen CD47 operabilno spojen s promotorom endogenog glodavčkog CD47, te gdje glodavačka stanica ili tkivo mogu biti mišja stanica ili mišje tkivo, ili štakorska stanica ili štakorsko tkivo.
5. Glodavačka stanica u skladu s patentnim zahtjevom 4, naznačena time što je navedena stanica glodavačka embrionska matična stanica, gdje glodavačka embrionska matična stanica može biti mišja ili štakorska embrionska matična stanica.
6. Glodavački embrij, naznačen time što se dobiva iz embrionske matične stanice u skladu s patentnim zahtjevom 5.
7. Postupak osiguravanja glodavca čiji genom sadrži gen CD47, koji kodira izvanstanični dio ljudskog polipeptida CD47 spojen s unutarstaničnim dijelom endogenog polipeptida CD47, naznačen time što se postupak sastoji u modificiranju genoma glodavca tako da sadrži humanizirani gen CD47, gdje humanizirani gen CD47 sadrži egzon 1 iz endogenog glodavačkog gena CD47, egzone 2-7 iz ljudskog gena CD47, te egzone nizvodno od egzona 7 iz endogenog glodavačkog gena CD47, gdje je humanizirani gen CD47 operabilno spojen s promotorom endogenog glodavčkog CD47, čime se osigurava navedeni glodavac.
8. Postupak dobivanja glodavca koji eksprimira polipeptid CD47 iz lokusa endogenog CD47, gdje polipeptid CD47 sadrži ljudski slijed, naznačen time što se postupak sastoji u: (a) umetanju genomskog fragmenta u lokus endogenog glodavčkog CD47 u glodavačkoj embrionskoj matičnoj stanici, gdje navedeni genomski fragment sadrži egzone 2-7 iz ljudskog gena CD47, čime se dobije humanizirani gen CD47 na lokusu endogenog glodavčkog CD47; (b) dobivanju glodavačke embrionske matične stanice koja sadrži humanizirani gen CD47 iz (a); i (c) dobivanju glodavca iz glodavačke embrionske matične stanice iz (b).
9. Postupak u skladu s patentnim zahtjevom 7 ili patentnim zahtjevom 8, naznačen time što je glodavac miš ili štakor.
10. Postupak procjenjivanja usađenosti ljudskih stanica, naznačen time što se navedeni postupak sastoji u procjenjivanju usađenosti ljudskih stanica u glodavca u skladu s bilo kojim od patentnih zahtjeva 1-3, gdje ljudske stanice mogu biti krvotvorne matične stanice.
11. Postupak procjenjivanja terapijske djelotvornosti lijeka koji cilja na ljudske stanice, naznačen time što se postupak sastoji u: primjeni kandidatnog lijeka na glodavcu u skladu s bilo kojim od patentnih zahtjeva 1-3, u kojeg je presađena jedna ili više ljudskih stanica; i praćenju ljudskih stanica u glodavcu kako bi se odredilo terapijsku djelotvornost kandidatnog lijeka.
12. Postupak procjenjivanja potiče li kandidatni modulator ljudskog proteina CD47 aglutiniranje eritrocita, naznačen time što se navedeni postupak sastoji u: inkubiranju eritrocita izoliranih iz glodavca u skladu s bilo kojim od patentnih zahtjeva 1-3 u prisutnosti kandidatnog modulatora; i procjenjivanju potiče li kandidatni modulator aglutiniranje eritrocita.
HRP20190634TT 2014-12-05 2019-04-02 Životinje koje nisu ljudi, koje imaju humanizirani gen 47 iz razlikovnog grozda HRP20190634T1 (hr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462087992P 2014-12-05 2014-12-05
PCT/US2015/062614 WO2016089692A1 (en) 2014-12-05 2015-11-25 Non-human animals having a humanized cluster of differentiation 47 gene
EP15813960.0A EP3086637B1 (en) 2014-12-05 2015-11-25 Non-human animals having a humanized cluster of differentiation 47 gene

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HRP20190634T1 true HRP20190634T1 (hr) 2019-08-09

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HRP20231707TT HRP20231707T1 (hr) 2014-12-05 2015-11-25 Životinje koje nisu ljudi, koje sadrže humanizirani gen iz diferencijacijske skupine 47
HRP20190634TT HRP20190634T1 (hr) 2014-12-05 2019-04-02 Životinje koje nisu ljudi, koje imaju humanizirani gen 47 iz razlikovnog grozda
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