HRP20180266T1 - Postupak priprave osnovnog medija za kulturu mezenhimnih matičnih stanica, osnovni medij za kulturu mezenhimnih matičnih stanica, te terapijsko sredstvo za stanice koje se uzgaja i diferencira uz njegovu upotrebu - Google Patents

Postupak priprave osnovnog medija za kulturu mezenhimnih matičnih stanica, osnovni medij za kulturu mezenhimnih matičnih stanica, te terapijsko sredstvo za stanice koje se uzgaja i diferencira uz njegovu upotrebu Download PDF

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Publication number
HRP20180266T1
HRP20180266T1 HRP20180266TT HRP20180266T HRP20180266T1 HR P20180266 T1 HRP20180266 T1 HR P20180266T1 HR P20180266T T HRP20180266T T HR P20180266TT HR P20180266 T HRP20180266 T HR P20180266T HR P20180266 T1 HRP20180266 T1 HR P20180266T1
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Croatia
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stem cells
mesenchymal stem
culture medium
basic culture
differentiated
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HRP20180266TT
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English (en)
Inventor
Dong-Sam Suh
Jun Keun Lee
Dong Il Chang
Min Jung Choi
Jang Hoon Kim
Ga Ram Kim
Cheong Ho Chang
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Sewon Cellontech Co., Ltd.
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Application filed by Sewon Cellontech Co., Ltd. filed Critical Sewon Cellontech Co., Ltd.
Publication of HRP20180266T1 publication Critical patent/HRP20180266T1/hr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Claims (2)

1. Napredni osnovni medij za kulturu mezenhimnih matičnih stanica, naznačen time što sadrži komponente i njihove odgovarajuće količine u mg/l, kao što su dane u sljedećoj tablici: [image]
2. Napredni osnovni medij za kulturu mezenhimnih matičnih stanica u skladu s patentnim zahtjevom 1, naznačen time što u navedenom mediju mezenhimne matične stanice pokazuju stanične imunološke karakteristike gdje se CD166, CD105, CD90, CD44, CD29, CD73, te HLA-ABC pozitivni površinski markeri eksprimiraju kod 80% ili više, osobito gdje se CD44, CD105, CD90, CD73, te CD166 pozitivni površinski markeri eksprimiraju kod 95% ili više, a CD31, CD34, CD45, CD80, te HLA-DR negativni površinski markeri se eksprimiraju kod 5% ili manje.
HRP20180266TT 2011-08-31 2018-02-13 Postupak priprave osnovnog medija za kulturu mezenhimnih matičnih stanica, osnovni medij za kulturu mezenhimnih matičnih stanica, te terapijsko sredstvo za stanice koje se uzgaja i diferencira uz njegovu upotrebu HRP20180266T1 (hr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020110087498A KR101138091B1 (ko) 2011-08-31 2011-08-31 중간엽 줄기세포 기본 배양 배지 조성방법, 중간엽 줄기세포 기본 배양 배지 및 이를 이용하여 배양분화된 세포치료제
PCT/KR2011/006582 WO2013032052A1 (ko) 2011-08-31 2011-09-06 중간엽 줄기세포 기본 배양 배지 조성방법, 중간엽 줄기세포 기본 배양 배지 및 이를 이용하여 배양분화된 세포치료제
EP11871607.5A EP2752484B1 (en) 2011-08-31 2011-09-06 Method for preparing a basic culture medium for mesenchymal stem cells, basic culture medium for mesenchymal stem cells, and cell therapeutic agent cultured and differentiated using same

Publications (1)

Publication Number Publication Date
HRP20180266T1 true HRP20180266T1 (hr) 2018-04-06

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Family Applications (1)

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HRP20180266TT HRP20180266T1 (hr) 2011-08-31 2018-02-13 Postupak priprave osnovnog medija za kulturu mezenhimnih matičnih stanica, osnovni medij za kulturu mezenhimnih matičnih stanica, te terapijsko sredstvo za stanice koje se uzgaja i diferencira uz njegovu upotrebu

Country Status (17)

Country Link
US (1) US9879226B2 (hr)
EP (1) EP2752484B1 (hr)
JP (1) JP5872046B2 (hr)
KR (1) KR101138091B1 (hr)
CN (1) CN103857789B (hr)
CY (1) CY1119924T1 (hr)
DK (1) DK2752484T3 (hr)
ES (1) ES2660898T3 (hr)
HR (1) HRP20180266T1 (hr)
HU (1) HUE036910T2 (hr)
LT (1) LT2752484T (hr)
NO (1) NO2752484T3 (hr)
PL (1) PL2752484T3 (hr)
PT (1) PT2752484T (hr)
SI (1) SI2752484T1 (hr)
TR (1) TR201802355T4 (hr)
WO (1) WO2013032052A1 (hr)

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CN108795853B (zh) * 2018-05-28 2021-08-24 天津博雅秀岩生物技术有限公司 制备犬胎膜间充质干细胞的方法和犬胎膜间充质干细胞
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JP7348892B2 (ja) * 2019-12-30 2023-09-21 佛教慈濟醫療財團法人 眼疾患を予防または治療する組成物及び方法
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PL2752484T3 (pl) 2018-06-29
DK2752484T3 (en) 2018-03-05
LT2752484T (lt) 2018-03-12
CY1119924T1 (el) 2018-12-12
EP2752484A4 (en) 2015-04-15
ES2660898T3 (es) 2018-03-26
US9879226B2 (en) 2018-01-30
JP2014525262A (ja) 2014-09-29
PT2752484T (pt) 2018-03-01
NO2752484T3 (hr) 2018-06-09
JP5872046B2 (ja) 2016-03-01
CN103857789A (zh) 2014-06-11
EP2752484A1 (en) 2014-07-09
HUE036910T2 (hu) 2018-08-28
KR101138091B1 (ko) 2012-04-24
SI2752484T1 (en) 2018-04-30
TR201802355T4 (tr) 2018-03-21
CN103857789B (zh) 2016-08-31
US20140370600A1 (en) 2014-12-18
EP2752484B1 (en) 2018-01-10
WO2013032052A1 (ko) 2013-03-07

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