WO2015064805A8 - 중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 연골세포로 분화시키는 방법 - Google Patents

중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 연골세포로 분화시키는 방법 Download PDF

Info

Publication number
WO2015064805A8
WO2015064805A8 PCT/KR2013/009953 KR2013009953W WO2015064805A8 WO 2015064805 A8 WO2015064805 A8 WO 2015064805A8 KR 2013009953 W KR2013009953 W KR 2013009953W WO 2015064805 A8 WO2015064805 A8 WO 2015064805A8
Authority
WO
WIPO (PCT)
Prior art keywords
stem cell
pluripotent stem
chondrocyte
induced
differentiating pluripotent
Prior art date
Application number
PCT/KR2013/009953
Other languages
English (en)
French (fr)
Other versions
WO2015064805A1 (ko
Inventor
이상연
정원주
김호빈
오민선
이계호
Original Assignee
주식회사 비비에이치씨
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP2016552370A priority Critical patent/JP6711760B2/ja
Priority to CN201380080663.2A priority patent/CN105705632B/zh
Priority to DK13896782.3T priority patent/DK3064574T3/da
Priority to ES13896782T priority patent/ES2773973T3/es
Application filed by 주식회사 비비에이치씨 filed Critical 주식회사 비비에이치씨
Priority to PL13896782T priority patent/PL3064574T3/pl
Priority to EP13896782.3A priority patent/EP3064574B1/en
Priority to LTEP13896782.3T priority patent/LT3064574T/lt
Priority to SI201331669T priority patent/SI3064574T1/sl
Priority to US15/033,358 priority patent/US9994820B2/en
Priority to RS20200146A priority patent/RS60193B1/sr
Priority to AU2013403888A priority patent/AU2013403888B2/en
Publication of WO2015064805A1 publication Critical patent/WO2015064805A1/ko
Publication of WO2015064805A8 publication Critical patent/WO2015064805A8/ko
Priority to CY20201100102T priority patent/CY1122798T1/el
Priority to HRP20200243TT priority patent/HRP20200243T1/hr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/76—Undefined extracts from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C12N2506/1369—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from blood-borne mesenchymal stem cells, e.g. MSC from umbilical blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Rheumatology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 감태 추출물(Ecklonia cava)을 포함하는 유도만능 줄기세포(induced pluripotency stem cell) 역분화용 배지 조성물에 관한 것이다. 또한, 본 발명은 상기 배지 조성물을 이용하여 제조된 유도만능 줄기세포로부터 연골세포로 분화시키는 방법에 관한 것이다. 본 발명에 따른 배지 조성물을 이용하면 중간엽 줄기세포를 이용하여 유도만능 줄기세포를 효율적으로 제조할 수 있으며, 제조된 만능 줄기세포는 연골세포로의 분화가 가능하므로 세포 치료제로서 유용하게 사용될 수 있다.
PCT/KR2013/009953 2013-11-01 2013-11-05 중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 연골세포로 분화시키는 방법 WO2015064805A1 (ko)

Priority Applications (13)

Application Number Priority Date Filing Date Title
EP13896782.3A EP3064574B1 (en) 2013-11-01 2013-11-05 Method for differentiating pluripotent stem cell induced from mesenchymal stem cell into chondrocyte
DK13896782.3T DK3064574T3 (da) 2013-11-01 2013-11-05 Fremgangsmåde til differentiering af pluripotente stamceller induceret fra mesenkymstamceller til chondrocyt
ES13896782T ES2773973T3 (es) 2013-11-01 2013-11-05 Método para diferenciar, células madre pluripotentes inducidas a partir de células madre mesenquimatosas, en condrocitos
SI201331669T SI3064574T1 (sl) 2013-11-01 2013-11-05 Postopek za diferenciranje pluripotentne matične celice, inducirane iz mezenhimske matične celice, v hondrocit
PL13896782T PL3064574T3 (pl) 2013-11-01 2013-11-05 Sposób różnicowania pluripotentnych komórek macierzystych indukowanych z mezenchymalnych komórek macierzystych w chondrocyt
CN201380080663.2A CN105705632B (zh) 2013-11-01 2013-11-05 将从间充质干细胞诱导的多能干细胞分化为软骨细胞的方法
LTEP13896782.3T LT3064574T (lt) 2013-11-01 2013-11-05 Pluripotentinių kamieninių ląstelių, indikuotų iš mezenchiminių kamieninių ląstelių, diferenciacijos į chondrocitus būdas
JP2016552370A JP6711760B2 (ja) 2013-11-01 2013-11-05 間葉系幹細胞から誘導された万能幹細胞を利用して軟骨細胞に分化させる方法
US15/033,358 US9994820B2 (en) 2013-11-01 2013-11-05 Method for differentiating pluripotent stem cell induced from mesenchymal stem cell into chondrocyte
RS20200146A RS60193B1 (sr) 2013-11-01 2013-11-05 Postupak za diferencijaciju pluripotentne matične ćelije indukovane iz mezenhimalne matične ćelije u hondrocit
AU2013403888A AU2013403888B2 (en) 2013-11-01 2013-11-05 Method for differentiating pluripotent stem cell induced from mesenchymal stem cell into chondrocyte
CY20201100102T CY1122798T1 (el) 2013-11-01 2020-02-04 Μεθοδος για τη διαφοροποιηση του πλειοδυναμου βλαστικου κυτταρου επαγομενη απο το μεσεγχυματικο βλαστικο κυτταρο σε χονδροκυτταρο
HRP20200243TT HRP20200243T1 (hr) 2013-11-01 2020-02-13 Postupak za razlikovanje pluripotentne matične stanice inducirane od mezenhimalne matične stanice u kondrocit

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2013-0132055 2013-11-01
KR1020130132055A KR101542846B1 (ko) 2013-11-01 2013-11-01 중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 연골세포로 분화시키는 방법

Publications (2)

Publication Number Publication Date
WO2015064805A1 WO2015064805A1 (ko) 2015-05-07
WO2015064805A8 true WO2015064805A8 (ko) 2016-07-21

Family

ID=53004393

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2013/009953 WO2015064805A1 (ko) 2013-11-01 2013-11-05 중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 연골세포로 분화시키는 방법

Country Status (17)

Country Link
US (1) US9994820B2 (ko)
EP (1) EP3064574B1 (ko)
JP (1) JP6711760B2 (ko)
KR (1) KR101542846B1 (ko)
CN (1) CN105705632B (ko)
AU (1) AU2013403888B2 (ko)
CY (1) CY1122798T1 (ko)
DK (1) DK3064574T3 (ko)
ES (1) ES2773973T3 (ko)
HR (1) HRP20200243T1 (ko)
HU (1) HUE048458T2 (ko)
LT (1) LT3064574T (ko)
PL (1) PL3064574T3 (ko)
PT (1) PT3064574T (ko)
RS (1) RS60193B1 (ko)
SI (1) SI3064574T1 (ko)
WO (1) WO2015064805A1 (ko)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
LT3064572T (lt) 2013-11-01 2020-07-27 Bbhc Co. Ltd. Indukuotų pluripotentinių kamieninių ląstelių gavimo būdas iš mezenchiminių kamieninių ląstelių ir indukuotos pluripotentinės kamieninės ląstelės, gautos taikant šį metodą
KR101699761B1 (ko) * 2014-05-23 2017-01-25 주식회사 비비에이치씨 플로로탄닌 분획물을 이용한 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하는 방법
CN109136178A (zh) * 2018-08-30 2019-01-04 丰泽康生物医药（深圳）有限公司 一种提高外周血多潜能细胞向软骨细胞分化的无血清培养基
WO2021037142A1 (en) * 2019-08-27 2021-03-04 The Third Hospital Of Hebei Medical University Multipotent joint progenitor cells, compositions and methods thereof
CN112662616A (zh) * 2021-01-27 2021-04-16 朱灏 一种高效率的人间充质干细胞诱导分化成软骨的方法
KR20230000033A (ko) * 2021-06-23 2023-01-02 연세대학교 산학협력단 위 세포 배양용 조성물
CN113652396B (zh) * 2021-08-24 2023-08-29 赛尔医学科技(山东)有限公司 一种脂肪间充质干细胞成骨诱导组合物及成骨诱导方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050084889A (ko) * 2002-10-31 2005-08-29 도꾸리쯔교세이호징 리가가쿠 겐큐소 다능성 줄기 세포 배양용 조성물 및 이의 용도
US20100135970A1 (en) 2006-10-27 2010-06-03 Caritas St. Elizabeth Medical Center Of Boston, In Methods for Reprogramming Adult Somatic Cells and Uses Thereof
WO2010003062A2 (en) * 2008-07-03 2010-01-07 The Regents Of The University Of California Biomaterials and implants for enhanced cartilage formation, and methods for making and using them
KR101127964B1 (ko) 2008-12-02 2012-04-16 한국전자통신연구원 사용자 적응형 연결 제어 장치 및 그를 포함하는 네트워크 시스템
KR101016761B1 (ko) 2009-02-26 2011-02-25 부경대학교 산학협력단 피부 미백활성을 갖는 감태 추출물
JP2012210154A (ja) 2009-08-03 2012-11-01 Keio Gijuku 分化細胞由来多能性幹細胞の樹立方法
WO2011037301A1 (ko) * 2009-09-22 2011-03-31 서울대학교병원 성체세포로부터 만능줄기세포를 유도하는 방법 및 그 방법에 의해 제조된 만능줄기세포
JP6263329B2 (ja) * 2009-11-24 2018-01-17 ザ ユニバーシティ オブ コネチカット ヒト胚性および人工多能性幹細胞の分化
KR20120002134A (ko) * 2010-06-30 2012-01-05 서울대학교산학협력단 지방 기질 세포의 역분화를 유도하는 방법
KR20120040488A (ko) 2010-10-19 2012-04-27 충남대학교산학협력단 곰피와 감태 추출물 유래의 플로로탄닌을 포함하는 화장품 조성물
KR101309538B1 (ko) 2010-11-17 2013-09-23 한양대학교 산학협력단 엑콜 화합물을 함유하는 암 줄기세포 성장 억제용 조성물
KR101887956B1 (ko) 2011-08-10 2018-08-13 (주)아모레퍼시픽 효소 처리 감태 추출물을 포함하는 피부 미백용 조성물

Also Published As

Publication number Publication date
US20160257936A1 (en) 2016-09-08
EP3064574A4 (en) 2017-09-06
KR20150050861A (ko) 2015-05-11
US9994820B2 (en) 2018-06-12
ES2773973T3 (es) 2020-07-15
AU2013403888A1 (en) 2016-06-16
KR101542846B1 (ko) 2015-08-10
CN105705632A (zh) 2016-06-22
SI3064574T1 (sl) 2020-07-31
DK3064574T3 (da) 2020-03-23
EP3064574A1 (en) 2016-09-07
PL3064574T3 (pl) 2020-07-27
WO2015064805A1 (ko) 2015-05-07
EP3064574B1 (en) 2020-01-22
RS60193B1 (sr) 2020-06-30
CY1122798T1 (el) 2021-05-05
JP2016537023A (ja) 2016-12-01
PT3064574T (pt) 2020-02-28
AU2013403888B2 (en) 2018-04-05
HRP20200243T1 (hr) 2020-09-04
JP6711760B2 (ja) 2020-06-17
CN105705632B (zh) 2021-09-14
HUE048458T2 (hu) 2020-07-28
LT3064574T (lt) 2020-05-25

Similar Documents

Publication Publication Date Title
WO2015064805A8 (ko) 중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 연골세포로 분화시키는 방법
HRP20180266T1 (hr) Postupak priprave osnovnog medija za kulturu mezenhimnih matičnih stanica, osnovni medij za kulturu mezenhimnih matičnih stanica, te terapijsko sredstvo za stanice koje se uzgaja i diferencira uz njegovu upotrebu
MX2016003444A (es) Formulaciones enzimaticas de rotura controlada.
GB2548740A (en) Methods for generation of podocytes from pluripotent stem cells and cells produced by the same
EP3235500A4 (en) Composition for chondrocyte differentiation induction or cartilage tissue regeneration, containing exosomes extracted from stem cells differentiating into chondrocytes
EP3070163A4 (en) Method for preparing induced pluripotent stem cell, composition used in method, and uses thereof
WO2012096461A3 (ko) 줄기세포의 부유배양용 조성물
CY1122791T1 (el) Μεθοδος για τη διαφοροποιηση του πλειοδυναμου βλαστικου κυτταρου επαγομενη απο το μεσεγχυματικο βλαστικο κυτταρο σε ηπατοκυτταρο
CY1122954T1 (el) Μεθοδος για τη διαφοροποιηση του πλειοδυναμου βλαστικου κυτταρου επαγομενη απο το μεσεγχυματικο βλαστικο κυτταρο σε νευρωνα
HK1222198A1 (zh) 用於提高幹細胞再生能力的培養基組合物及使用它的幹細胞培養方法
PH12016502330B1 (en) Method for preparing induced pluripotency stem cells from mesenchymal stem cells by using phlorotannin fraction
SG10201903910RA (en) Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same
MX2018010828A (es) Medio para formar colonias y uso de este.
WO2015121859A8 (en) Method of qualifying cells
EP3853345A4 (en) PROCEDURE FOR DIFFERENTIATION OF PLURIPOTENT STEM CELLS IN A DYNAMIC SUSPENSION CULTURE
WO2011100286A3 (en) Compositions and methods of generating a differentiated mesodermal cell
WO2015073625A3 (en) Synergistic genome-nonintegrating reprogramming by micrornas and transcription factors
CY1122842T1 (el) Μεθοδος για τη διαφοροποιηση του πλειοδυναμου βλαστικου κυτταρου επαγομενη απο το μεσεγχυματικο βλαστικο κυτταρο σε οστεοβλαστες
EP3511410A4 (en) MEDIUM FOR THE CULTURE OF STEM CELLS, METHOD FOR THE CULTURE OF STEM CELLS, STEM CELL GROWTH PROMOTER AND COMPOSITION AND METHOD FOR THE PRODUCTION THEREOF
EA201791233A1 (ru) Материал стволовых клеток и способ его получения
MY186719A (en) Metal recovery agent, metal compound recovery agent, and method for recovering metal or metal compound
EP3064570A4 (en) Method for differentiating pluripotent stem cell induced from mesenchymal stem cell into adipocyte
WO2016201182A8 (en) Muscular dystrophy chimeric cells and method for treating muscular dystrophies
TW201613623A (en) Methods to enhance nerve regeneration utilizing neural stem cells and IL12p40
CY1123057T1 (el) Μεθοδος για παραγωγη επαγομενου πολυδυναμου βλαστοκυτταρου απο μεσεγχυματικο βλαστοκυτταρο και επαγομενου πολυδυναμου βλαστοκυτταρου που παραγεται με τη μεθοδο

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 13896782

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 15033358

Country of ref document: US

ENP Entry into the national phase

Ref document number: 2016552370

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

REEP Request for entry into the european phase

Ref document number: 2013896782

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2013896782

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2013403888

Country of ref document: AU

Date of ref document: 20131105

Kind code of ref document: A