HRP20171411T1 - Postupak za detektiranje karcinoma grlića maternice prouzročenog humanim papiloma virusom (hpv) - Google Patents
Postupak za detektiranje karcinoma grlića maternice prouzročenog humanim papiloma virusom (hpv) Download PDFInfo
- Publication number
- HRP20171411T1 HRP20171411T1 HRP20171411TT HRP20171411T HRP20171411T1 HR P20171411 T1 HRP20171411 T1 HR P20171411T1 HR P20171411T T HRP20171411T T HR P20171411TT HR P20171411 T HRP20171411 T HR P20171411T HR P20171411 T1 HRP20171411 T1 HR P20171411T1
- Authority
- HR
- Croatia
- Prior art keywords
- hpv
- methylation
- caused
- nucleic acid
- detecting
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims 10
- 206010008342 Cervix carcinoma Diseases 0.000 title 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 title 1
- 201000010881 cervical cancer Diseases 0.000 title 1
- 208000022361 Human papillomavirus infectious disease Diseases 0.000 claims 11
- 230000011987 methylation Effects 0.000 claims 8
- 238000007069 methylation reaction Methods 0.000 claims 8
- 239000000523 sample Substances 0.000 claims 7
- 230000003902 lesion Effects 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 206010028980 Neoplasm Diseases 0.000 claims 3
- 102100032120 Toll/interleukin-1 receptor domain-containing adapter protein Human genes 0.000 claims 3
- 201000011510 cancer Diseases 0.000 claims 3
- 108091008146 restriction endonucleases Proteins 0.000 claims 3
- 101150108610 MAL gene Proteins 0.000 claims 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims 2
- 210000003679 cervix uteri Anatomy 0.000 claims 2
- 208000024312 invasive carcinoma Diseases 0.000 claims 2
- 239000002853 nucleic acid probe Substances 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 101000697844 Arabidopsis thaliana Thiosulfate sulfurtransferase 16, chloroplastic Proteins 0.000 claims 1
- 201000009030 Carcinoma Diseases 0.000 claims 1
- 208000009608 Papillomavirus Infections Diseases 0.000 claims 1
- 210000001744 T-lymphocyte Anatomy 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 239000013068 control sample Substances 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 108091056924 miR-124 stem-loop Proteins 0.000 claims 1
- 239000002243 precursor Substances 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Hospice & Palliative Care (AREA)
- Biophysics (AREA)
- Oncology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
Claims (12)
1. Postupak detektiranja pojave HPV-om prouzročenih prekancerogenih lezija grlića materice visokog stupnja i/ili HPV-om prouzročenih invazivnih karcinoma grlića materice koji obuhvaćaju oglede cervikovaginalnog uzorka lavaže na prisutnost promjene u metilaciji proteina povezanog sa starenjem (MAL) T limfocita i hsa-miR124, pri čemu navedena promjena označava prisutnost HPV-om uzrokovanih prekursorskih lezija s invazivnim potencijalom i/ili HPV-om prouzročenim invazivnih karcinoma.
2. Postupak sukladno patentnom zahtjevu 1, pri tome je navedeni HPV-om prouzročeni karcionom HPV-om prouzročen invazivni karcinom visokog rizika.
3. Postupak iz bilo kojeg od prethodnih patentnih zahtjeva pri čemu je navedena promjena povećanje metilacije u usporedbi s normalnim kontrolnim uzorkom.
4. Postupak sukladno bilo kojem od prethodnih patentnih zahtjeva, pri čemu je nukleinska kiselina DNK.
5. Postupak sukladno patentnom zahtjevu 4, pri čemu se ovaj ogled izvodi s restriktivnom endonukleazom, preporučljivo restriktivnom endonukleazom osjetljivom na metilaciju.
6. Postupak sukladno patentnom zahtjevu 4, pri čemu je reagens funkcijska sonda nukleinske kiseline ili početnica koja se veže na nukleinsku kiselinu.
7. Postupak sukladno patentnom zahtjevu 6, pri čemu sukladno funkcijska sonda nukleinske kiseline ili početnica nukleinske kiseline ima detektibilnu oznaku.
8. Postupak sukladno patentnom zahtjevu 6 ili 7, pri čemu ta funkcijska sonda nukleinske kiseline ima sekvencu nukleotida odabranu iz skupine koja sadrži sekvence prikazane u Tablici 1.
9. Upotreba molekularnog dijagnostičkog markera podešenih za detektiranje HPV-om prouzročenih pretkancerogenih lezija visokog rizika ili HPV-om prouzročenih invazivnog karcinoma u uzorku cerviko-vaginalne lavaže, pri čemu navedeni komplet markera označava promjenu u metilaciji MAL gena ili promotoru i promjeni za metilaciju hsa-miR124 sekvence.
10. Upotreba kompleta dijelova u postupku detektiranja HPV-om prouzročene prekancerogene lezije visokog rizika ili HPV-om prouzročenog invazivnog karcinoma u testiranim stanicama iz uzorka cervikovaginalne lavaže ispitanika, navedeni komplet sadrži na metilaciju osjetljivu restriktivnu endonukleazu i
- sredstva za detekiranje metilacije MAL gena ili promotora pri tome navedena sredstva sadrže funkcijske sonde, početnice specifične za MAL ili specifične za MAL sekvencu nukleotida sa Slike 1, i
- sredstva za detektiranje hsa-miR124 metilacije, pri tome navedena sredstva sadrže funkcijske sonde, primere specifične za hsa-miR124.
11. Upotreba sukaldno patentnom zahtjevu 10, pri tome navedeni komplet dijelova još sadrži sredstva za detektiranje HPV infekcije, pri tome navedena sredstva sadrže funkcijske sonde i početnice specifične za HPV.
12. Upotreba kompleta dijelova kako je definirano u patentnim zahtjevima 10 ili 11, još sadrži sredstva za uzimanje cervikovaginalne lavaže.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11181492 | 2011-09-15 | ||
| PCT/NL2012/050645 WO2013039394A1 (en) | 2011-09-15 | 2012-09-13 | Methylation analysis on self-samples as triage tool for hpv-positive women |
| EP12783347.3A EP2756099B1 (en) | 2011-09-15 | 2012-09-13 | Method to detect HPV-induced cervical cancer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20171411T1 true HRP20171411T1 (hr) | 2017-11-17 |
Family
ID=47144020
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20171411TT HRP20171411T1 (hr) | 2011-09-15 | 2017-09-18 | Postupak za detektiranje karcinoma grlića maternice prouzročenog humanim papiloma virusom (hpv) |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US20150225796A1 (hr) |
| EP (1) | EP2756099B1 (hr) |
| JP (1) | JP6328051B2 (hr) |
| CN (1) | CN104053783A (hr) |
| AU (1) | AU2012309226B2 (hr) |
| CA (1) | CA2848654C (hr) |
| CY (1) | CY1119570T1 (hr) |
| DK (1) | DK2756099T3 (hr) |
| ES (1) | ES2641093T3 (hr) |
| HK (1) | HK1198774A1 (hr) |
| HR (1) | HRP20171411T1 (hr) |
| HU (1) | HUE034556T2 (hr) |
| LT (1) | LT2756099T (hr) |
| PL (1) | PL2756099T3 (hr) |
| PT (1) | PT2756099T (hr) |
| RS (1) | RS56380B1 (hr) |
| SI (1) | SI2756099T1 (hr) |
| WO (1) | WO2013039394A1 (hr) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2013330509B2 (en) | 2012-10-12 | 2019-01-17 | Self-Screen B.V. | PRDM14 and FAM19A4, molecular diagnostic markers for HPV-induced invasive cancers and their high-grade precursor lesions |
| EP2757161A1 (en) * | 2013-01-17 | 2014-07-23 | Splicos | miRNA-124 as a biomarker of viral infection |
| JP2016201999A (ja) * | 2013-08-26 | 2016-12-08 | 北海道公立大学法人 札幌医科大学 | 大腸癌を検出する方法 |
| JP6607444B2 (ja) * | 2014-02-04 | 2019-11-20 | 公立大学法人横浜市立大学 | 子宮頸がんの前がん病変の進行を予測するための方法 |
| EP2974729A1 (en) | 2014-07-17 | 2016-01-20 | Abivax | Quinoline derivatives for use in the treatment of inflammatory diseases |
| CN113564250A (zh) * | 2014-10-17 | 2021-10-29 | 科罗拉多大学董事会法人团体 | 头颈部癌症的生物标志物及其使用方法 |
| EP3135768A1 (en) * | 2015-08-26 | 2017-03-01 | Self-screen B.V. | Zic1 and ghsr, molecular diagnostic markers for hpv-induced invasive cancers, nonhpv-induced gynaecological and anogenital cancers and their high-grade precursor lesions |
| SG10202106932YA (en) * | 2017-03-10 | 2021-08-30 | Self Screen Bv | A methylation classifier for detection of hpv-induced invasive cancers, nonhpv- induced gynaecological and anogenital cancers and their high-grade precursor lesions |
| JP7218887B2 (ja) * | 2017-06-29 | 2023-02-07 | 学校法人藤田学園 | 子宮頸がん検査用検体 |
| US20200216911A1 (en) * | 2017-09-29 | 2020-07-09 | Oncgnostics Gmbh | Risk determination for neoplasia and cancer |
| CN107760788B (zh) * | 2017-12-05 | 2021-03-02 | 武汉艾米森生命科技有限公司 | 一种检测宫颈细胞基因甲基化的核酸组合及试剂盒与应用 |
| EP3821038A1 (en) * | 2018-07-11 | 2021-05-19 | Stichting VUmc | Urine dna methylation markers for bladder cancer |
| EP3669873A1 (en) | 2018-12-20 | 2020-06-24 | Abivax | Quinoline derivatives for use ine the traeatment of inflammation diseases |
| CN114214415A (zh) * | 2021-12-29 | 2022-03-22 | 广州安必平医药科技股份有限公司 | 一种用于宫颈癌相关基因甲基化检测的引物探针组合及其应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009036332A1 (en) * | 2007-09-14 | 2009-03-19 | Asuragen, Inc. | Micrornas differentially expressed in cervical cancer and uses thereof |
| WO2009113859A1 (en) * | 2008-03-13 | 2009-09-17 | Vereniging Voor Christelijk Hoger Onderwijs, Wetenschappelijk Onderzoek En Patiëntenzorg | Cervical screening algorithms |
| CA2721356C (en) * | 2008-04-14 | 2019-11-12 | Vereniging Voor Christelijk Hoger Onderwijs, Wetenschappelijk Onderzoeen Patieentenzorg | Mal, a molecular diagnostic marker for hpv-induced invasive cancers and their high-grade precursor lesions |
-
2012
- 2012-09-13 EP EP12783347.3A patent/EP2756099B1/en active Active
- 2012-09-13 RS RS20170925A patent/RS56380B1/sr unknown
- 2012-09-13 HU HUE12783347A patent/HUE034556T2/en unknown
- 2012-09-13 LT LTEP12783347.3T patent/LT2756099T/lt unknown
- 2012-09-13 ES ES12783347.3T patent/ES2641093T3/es active Active
- 2012-09-13 CA CA2848654A patent/CA2848654C/en active Active
- 2012-09-13 PT PT127833473T patent/PT2756099T/pt unknown
- 2012-09-13 SI SI201231059T patent/SI2756099T1/sl unknown
- 2012-09-13 DK DK12783347.3T patent/DK2756099T3/en active
- 2012-09-13 US US14/344,921 patent/US20150225796A1/en not_active Abandoned
- 2012-09-13 PL PL12783347T patent/PL2756099T3/pl unknown
- 2012-09-13 JP JP2014530620A patent/JP6328051B2/ja not_active Expired - Fee Related
- 2012-09-13 CN CN201280055566.3A patent/CN104053783A/zh active Pending
- 2012-09-13 WO PCT/NL2012/050645 patent/WO2013039394A1/en active Application Filing
- 2012-09-13 AU AU2012309226A patent/AU2012309226B2/en not_active Ceased
-
2014
- 2014-12-04 HK HK14112234.5A patent/HK1198774A1/xx not_active IP Right Cessation
-
2017
- 2017-09-18 HR HRP20171411TT patent/HRP20171411T1/hr unknown
- 2017-09-20 CY CY20171100986T patent/CY1119570T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN104053783A (zh) | 2014-09-17 |
| CA2848654C (en) | 2021-07-20 |
| JP2014526907A (ja) | 2014-10-09 |
| CA2848654A1 (en) | 2013-03-21 |
| LT2756099T (lt) | 2017-10-10 |
| PL2756099T3 (pl) | 2017-12-29 |
| CY1119570T1 (el) | 2018-03-07 |
| RS56380B1 (sr) | 2017-12-29 |
| EP2756099A1 (en) | 2014-07-23 |
| DK2756099T3 (en) | 2017-10-02 |
| SI2756099T1 (sl) | 2017-11-30 |
| ES2641093T3 (es) | 2017-11-07 |
| WO2013039394A1 (en) | 2013-03-21 |
| HUE034556T2 (en) | 2018-02-28 |
| JP6328051B2 (ja) | 2018-05-30 |
| AU2012309226A1 (en) | 2014-04-03 |
| PT2756099T (pt) | 2017-09-26 |
| US20150225796A1 (en) | 2015-08-13 |
| AU2012309226B2 (en) | 2017-12-14 |
| EP2756099B1 (en) | 2017-06-28 |
| HK1198774A1 (en) | 2015-06-05 |
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