HK1250747A1 - 用於次要等位基因和多态性的监定和定量的多重化方法 - Google Patents

用於次要等位基因和多态性的监定和定量的多重化方法 Download PDF

Info

Publication number
HK1250747A1
HK1250747A1 HK18110098.0A HK18110098A HK1250747A1 HK 1250747 A1 HK1250747 A1 HK 1250747A1 HK 18110098 A HK18110098 A HK 18110098A HK 1250747 A1 HK1250747 A1 HK 1250747A1
Authority
HK
Hong Kong
Prior art keywords
nucleic acid
acid species
chain terminating
minor
major
Prior art date
Application number
HK18110098.0A
Other languages
English (en)
Chinese (zh)
Inventor
Anders Olof Herman Nygren
Original Assignee
Agena Bioscience, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agena Bioscience, Inc. filed Critical Agena Bioscience, Inc.
Publication of HK1250747A1 publication Critical patent/HK1250747A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
HK18110098.0A 2015-04-24 2016-04-22 用於次要等位基因和多态性的监定和定量的多重化方法 HK1250747A1 (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562152697P 2015-04-24 2015-04-24
US62/152,697 2015-04-24
US201662280951P 2016-01-20 2016-01-20
US62/280,951 2016-01-20
PCT/US2016/028971 WO2016172571A1 (en) 2015-04-24 2016-04-22 Multiplexed method for the identification and quantitation of minor alleles and polymorphisms

Publications (1)

Publication Number Publication Date
HK1250747A1 true HK1250747A1 (zh) 2019-01-11

Family

ID=55911096

Family Applications (1)

Application Number Title Priority Date Filing Date
HK18110098.0A HK1250747A1 (zh) 2015-04-24 2016-04-22 用於次要等位基因和多态性的监定和定量的多重化方法

Country Status (8)

Country Link
US (5) US10233489B2 (https=)
EP (2) EP4001430A1 (https=)
JP (5) JP2018512880A (https=)
CN (2) CN114540470B (https=)
AU (1) AU2016250841A1 (https=)
CA (1) CA2983224A1 (https=)
HK (1) HK1250747A1 (https=)
WO (1) WO2016172571A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6040227B2 (ja) 2011-05-19 2016-12-07 アジーナ バイオサイエンス， インコーポレイテッド マルチプレックス核酸同定のための製品およびプロセス
US10640817B2 (en) 2015-04-24 2020-05-05 Agena Bioscience, Inc. Multiplex methods for detection and quantification of minor variants
AU2016250841A1 (en) 2015-04-24 2017-11-02 Agena Bioscience, Inc. Multiplexed method for the identification and quantitation of minor alleles and polymorphisms
US12188083B2 (en) 2018-06-01 2025-01-07 Agena Bioscience, Inc. Products and processes for nucleic acid detection and quantification
CN113466320B (zh) * 2021-08-06 2022-10-14 河北省食品检验研究院 一种针对乳粉中牛源性成分maldi-tof检测方法
KR102857507B1 (ko) * 2022-11-14 2025-09-10 명지대학교 산학협력단 무의 근피 색상 판별용 snp 마커 및 이의 용도
US12518859B2 (en) * 2023-07-26 2026-01-06 Helix, Inc. Systems and methods for providing test results of gene sequencing data on a recurring basis

Family Cites Families (93)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
DE3301833A1 (de) 1983-01-20 1984-07-26 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Verfahren zur simultanen synthese mehrerer oligonocleotide an fester phase
US5059654A (en) 1983-02-14 1991-10-22 Cuno Inc. Affinity matrices of modified polysaccharide supports
US4515781A (en) 1983-02-23 1985-05-07 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services 2',5'-Riboadenylate-morpholinoadenylate nucleotides
US4582789A (en) 1984-03-21 1986-04-15 Cetus Corporation Process for labeling nucleic acids using psoralen derivatives
US4957858A (en) 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
US5118605A (en) 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
JPH0779717B2 (ja) 1984-10-29 1995-08-30 マイクロジエニツクス コ−ポレ−シヨン タンパク質結合酵素相補性検定に関する方法
US5064754A (en) 1984-12-14 1991-11-12 Mills Randell L Genomic sequencing method
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
EP0260032B1 (en) 1986-09-08 1994-01-26 Ajinomoto Co., Inc. Compounds for the cleavage at a specific position of RNA, oligomers employed for the formation of said compounds, and starting materials for the synthesis of said oligomers
EP0269520A3 (fr) 1986-11-21 1988-08-24 Institut Pasteur Rétrovirus du type HIV-2 susceptible de provoquer le sida, et ses constituants antigéniques et nucléiques
US5037882A (en) 1987-03-11 1991-08-06 Steel Samuel L Synthesis of oligonucleotide analogs
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
ATE241014T2 (de) 1988-06-14 2003-06-15 Qiagen Gmbh Hiv-2 varianten
US5003059A (en) 1988-06-20 1991-03-26 Genomyx, Inc. Determining DNA sequences by mass spectrometry
US5512439A (en) 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
US5489507A (en) 1988-11-30 1996-02-06 Perkin-Elmer Corporation DNA detection by color complementation
US5237016A (en) 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
US5242974A (en) 1991-11-22 1993-09-07 Affymax Technologies N.V. Polymer reversal on solid surfaces
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
WO1991001384A1 (en) 1989-07-11 1991-02-07 Gen-Probe, Incorporated Nucleic acid sequence amplification methods utilizing a transcription complex
WO1991011533A1 (en) 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Method for isolating primer extension products from template-directed dna polymerase reactions
US5650489A (en) 1990-07-02 1997-07-22 The Arizona Board Of Regents Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof
WO1992011390A1 (en) 1990-12-17 1992-07-09 Idexx Laboratories, Inc. Nucleic acid sequence detection by triple helix formation
US5364759B2 (en) 1991-01-31 1999-07-20 Baylor College Medicine Dna typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
US5888819A (en) 1991-03-05 1999-03-30 Molecular Tool, Inc. Method for determining nucleotide identity through primer extension
EP0636186B1 (en) 1992-04-03 1998-11-25 The Perkin-Elmer Corporation Probe composition and method
US5412083A (en) 1992-04-16 1995-05-02 Northeastern University Carbohydrate heterobifunctional cross-linking reagent
WO1994000562A1 (en) 1992-06-24 1994-01-06 The Mt. Sinai School Of Medicine Of The City University Of New York A novel human immunodeficiency virus
US5710028A (en) 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
DE69433811T2 (de) 1993-01-07 2005-06-23 Sequenom, Inc., San Diego Dns - sequenzierung durch massenspektronomie
US6194144B1 (en) 1993-01-07 2001-02-27 Sequenom, Inc. DNA sequencing by mass spectrometry
US6074823A (en) 1993-03-19 2000-06-13 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
CA2158642A1 (en) 1993-03-19 1994-09-29 Hubert Koster Dna sequencing by mass spectrometry via exonuclease degradation
US5399857A (en) 1993-05-28 1995-03-21 The Johns Hopkins University Method and apparatus for trapping ions by increasing trapping voltage during ion introduction
JPH07159404A (ja) 1993-12-06 1995-06-23 Doujin Kagaku Kenkyusho:Kk スピンラベルしたビオチンを用いた物質の測定方法
US5976798A (en) 1994-03-30 1999-11-02 Mitokor Methods for detecting mitochondrial mutations diagnostic for Alzheimer's disease and methods for determining heteroplasmy of mitochondrial nucleic acid
GB9417211D0 (en) 1994-08-25 1994-10-12 Solicitor For The Affairs Of H Nucleotide sequencing method
AU708375B2 (en) 1995-02-09 1999-08-05 University Of Washington Modified-affinity streptavidin
US6428955B1 (en) 1995-03-17 2002-08-06 Sequenom, Inc. DNA diagnostics based on mass spectrometry
AU5296496A (en) 1995-03-24 1996-10-16 Mitokor Mutation detection by differential primer extension of mutant and wildtype target sequences
AU5544696A (en) 1995-04-11 1996-10-30 Trustees Of Boston University Solid phase sequencing of biopolymers
US5770272A (en) 1995-04-28 1998-06-23 Massachusetts Institute Of Technology Matrix-bearing targets for maldi mass spectrometry and methods of production thereof
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5869242A (en) 1995-09-18 1999-02-09 Myriad Genetics, Inc. Mass spectrometry to assess DNA sequence polymorphisms
WO1997037041A2 (en) 1996-03-18 1997-10-09 Sequenom, Inc. Dna sequencing by mass spectrometry
US5965363A (en) 1996-09-19 1999-10-12 Genetrace Systems Inc. Methods of preparing nucleic acids for mass spectrometric analysis
US5885775A (en) 1996-10-04 1999-03-23 Perseptive Biosystems, Inc. Methods for determining sequences information in polynucleotides using mass spectrometry
EP1164203B1 (en) 1996-11-06 2007-10-10 Sequenom, Inc. DNA Diagnostics based on mass spectrometry
EP0963443B1 (en) 1996-12-10 2006-03-08 Sequenom, Inc. Releasable nonvolatile mass-label molecules
US6613509B1 (en) 1999-03-22 2003-09-02 Regents Of The University Of California Determination of base (nucleotide) composition in DNA oligomers by mass spectrometry
US20050287592A1 (en) 2000-08-29 2005-12-29 Yeda Research And Development Co. Ltd. Template-dependent nucleic acid polymerization using oligonucleotide triphosphates building blocks
GB0006141D0 (en) 2000-03-14 2000-05-03 Brax Group Ltd Mass labels
EP1176212A1 (en) 2000-07-24 2002-01-30 Centre National de Genotype Method for haplotyping by mass spectrometry
US20030027169A1 (en) * 2000-10-27 2003-02-06 Sheng Zhang One-well assay for high throughput detection of single nucleotide polymorphisms
GB0102568D0 (en) 2001-02-01 2001-03-21 Magnetic Biosolutions Sweden A Method
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US20040121314A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in containers
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
EP1423400B2 (en) 2001-03-19 2013-05-22 President and Fellows of Harvard College Evolving new molecular function
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
US20030119004A1 (en) 2001-12-05 2003-06-26 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification
JP3752466B2 (ja) 2002-04-24 2006-03-08 株式会社日立製作所 遺伝子検査方法
WO2003104420A2 (en) 2002-06-07 2003-12-18 The Regents Of The University Of California Electrochemical detection of single nucleotide polymorphisms (snps)
US7074597B2 (en) 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
DE10240746A1 (de) 2002-09-01 2004-03-18 Epigenomics Ag Verfahren zum Nachweis von Nukleinsäuresequenzen mittels spaltbarer Sondenmoleküle
US20040259105A1 (en) * 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
EP2107129B1 (en) 2003-07-31 2012-08-22 Sequenom, Inc. Methods for high level multiplexed polymerase chain reactions and homogeneous mass extension reactions for genotyping of polymorhisms
US7709262B2 (en) 2004-02-18 2010-05-04 Trustees Of Boston University Method for detecting and quantifying rare mutations/polymorphisms
CN100400673C (zh) * 2004-03-18 2008-07-09 华晶基因技术有限公司 单核苷酸多态性（SNPs）及点突变的检测方法
WO2006073436A2 (en) 2004-04-29 2006-07-13 The Trustees Of Columbia University In The City Of New York Mass tag pcr for multiplex diagnostics
EP1756307A1 (en) 2004-05-20 2007-02-28 Trillion Genomics Limited Use of mass labelled probes to detect target nucleic acids using mass spectrometry
JP4735926B2 (ja) 2004-05-27 2011-07-27 アイシン精機株式会社 核酸の高効率回収方法
US7785843B2 (en) 2004-06-23 2010-08-31 Sequenom, Inc. Target-specific compomers and methods of use
US20060194240A1 (en) 2005-02-28 2006-08-31 Gen-Probe Incorporated Compositions and methods of detecting an analyte by using a nucleic acid hybridization switch probe
JP2006320271A (ja) 2005-05-20 2006-11-30 Tokyo Institute Of Technology 低分子化合物とタンパク質の結合評価方法
JP2008545444A (ja) 2005-06-13 2008-12-18 エフ．ホフマン−ラ ロシュ アーゲー 急性拒絶反応に関連するｉｌ１０ｓｎｐ
US8586708B2 (en) 2005-10-28 2013-11-19 Massachusetts Institute Of Technology Monovalent streptavidin compositions
US8133701B2 (en) 2006-12-05 2012-03-13 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
US9249423B2 (en) 2007-02-02 2016-02-02 Yale University Method of de-differentiating and re-differentiating somatic cells using RNA
WO2009046507A1 (en) 2007-10-10 2009-04-16 Atanas Atanasov Wave power station
CA2742272C (en) 2008-10-30 2018-05-29 Sequenom, Inc. Products and processes for multiplex nucleic acid identification
DK2540843T3 (da) 2008-11-05 2014-09-01 Genentech Inc Genetiske polymorfismer ved aldersbetinget makulær degeneration
JP5686098B2 (ja) 2009-09-17 2015-03-18 Jsr株式会社 アビジンとビオチン誘導体の解離方法及び解離剤
JP6040227B2 (ja) 2011-05-19 2016-12-07 アジーナ バイオサイエンス， インコーポレイテッド マルチプレックス核酸同定のための製品およびプロセス
CA2877493C (en) * 2012-07-24 2020-08-25 Natera, Inc. Highly multiplex pcr methods and compositions
CN103131787B (zh) * 2013-03-11 2014-05-21 四川大学 基于y染色体snp遗传标记的法医学复合检测试剂盒
CN103602740A (zh) * 2013-11-06 2014-02-26 毅新兴业（北京）科技有限公司 质谱仪鉴定血清中游离dna的方法、试剂盒及其应用
US10640817B2 (en) 2015-04-24 2020-05-05 Agena Bioscience, Inc. Multiplex methods for detection and quantification of minor variants
AU2016250841A1 (en) 2015-04-24 2017-11-02 Agena Bioscience, Inc. Multiplexed method for the identification and quantitation of minor alleles and polymorphisms

Also Published As

Publication number Publication date
JP2018512880A (ja) 2018-05-24
CA2983224A1 (en) 2016-10-27
WO2016172571A1 (en) 2016-10-27
JP2019083820A (ja) 2019-06-06
AU2016250841A1 (en) 2017-11-02
JP2021097719A (ja) 2021-07-01
EP3286327A1 (en) 2018-02-28
JP2021097718A (ja) 2021-07-01
CN107406882A (zh) 2017-11-28
JP2024012522A (ja) 2024-01-30
US10233489B2 (en) 2019-03-19
US20160312278A1 (en) 2016-10-27
US10865439B2 (en) 2020-12-15
US20230383340A1 (en) 2023-11-30
CN114540470A (zh) 2022-05-27
US20210062254A1 (en) 2021-03-04
US10513728B2 (en) 2019-12-24
CN114540470B (zh) 2026-01-30
JP7325467B2 (ja) 2023-08-14
US11680289B2 (en) 2023-06-20
EP3286327B1 (en) 2021-11-10
EP4001430A1 (en) 2022-05-25
US20200123601A1 (en) 2020-04-23
US20190153526A1 (en) 2019-05-23
US12454722B2 (en) 2025-10-28
CN107406882B (zh) 2022-03-01

Similar Documents

Publication Publication Date Title
HK1250747A1 (zh) 用於次要等位基因和多态性的监定和定量的多重化方法
Navarro et al. Real-time PCR detection chemistry
WO2013177265A8 (en) A QUANTITATIVE MULTIPLEX METHYLATION SPECIFIC PCR METHOD- cMethDNA, REAGENTS, AND ITS USE
Flores et al. Internal validation of the GlobalFiler™ Express PCR Amplification Kit for the direct amplification of reference DNA samples on a high-throughput automated workflow
EP4484577A3 (en) Digital analyte analysis
Zhang et al. Development of a new 26plex Y-STRs typing system for forensic application
HK1248286A1 (zh) 血浆dna的单分子测序
ZA202003919B (en) Loop-mediated isothermal amplification (lamp) based assay for detecting microbes
WO2013022961A8 (en) Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
MY149386A (en) A method for identifying a pork content in a food
WO2005089508A3 (en) Dna sequence detection by limited primer extension
EP3543362A3 (en) Pcr primer set for bacterial dna amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species
WO2010146339A8 (en) Nucleic acid detection
JP2016512437A5 (https=)
EA038921B9 (ru) Обнаружение целевой нуклеиновой кислоты и ее вариантов
WO2012061502A3 (en) Genetic polymorphisms associated with venous thrombosis and statin response, methods of detection and uses thereof
RU2014126423A (ru) Высокопроизводительный анализ однонуклеотидных полиморфизмов
WO2017160779A3 (en) Methods and kits to identify klebsiella strains
NZ598008A (en) An assay for determining a molecular risk assessment of a complex polymicrobial sample suspected to contain an ehec
NZ732886A (en) Amplification and detection of nucleic acids in a biological sample
CA3077390A1 (en) Methods and compositions for nucleic acid detection
WO2015009844A3 (en) Mirror bisulfite analysis
WO2011135058A3 (en) Methods for detecting epigenetic modifications
MY174088A (en) A method of detecting porcine markers for identifying pork content in a sample
MX2019011503A (es) Composiciones de cebador y métodos multiplex para detectar la presencia de listeria.