WO2011135058A3 - Methods for detecting epigenetic modifications - Google Patents

Methods for detecting epigenetic modifications Download PDF

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Publication number
WO2011135058A3
WO2011135058A3 PCT/EP2011/056797 EP2011056797W WO2011135058A3 WO 2011135058 A3 WO2011135058 A3 WO 2011135058A3 EP 2011056797 W EP2011056797 W EP 2011056797W WO 2011135058 A3 WO2011135058 A3 WO 2011135058A3
Authority
WO
WIPO (PCT)
Prior art keywords
sample
gene
methylation status
dna
methods
Prior art date
Application number
PCT/EP2011/056797
Other languages
French (fr)
Other versions
WO2011135058A2 (en
Inventor
Joost Louwagie
Gaetan Otto
Original Assignee
Mdxhealth Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mdxhealth Sa filed Critical Mdxhealth Sa
Publication of WO2011135058A2 publication Critical patent/WO2011135058A2/en
Publication of WO2011135058A3 publication Critical patent/WO2011135058A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of determining the methylation status of a gene, in particular at least SYNE1 in a sample, such as a blood sample, in particular a blood plasma or serum sample, comprises the following steps: (a) isolating DNA from the sample (which may be a blood sample, in particular a plasma or serum sample) (b) subjecting the isolated DNA to treatment with a reagent which selectively modifies unmethylated cytosine residues in the DNA contained in the sample to produce detectable modified residues but which does not modify methylated cytosine residues (c) amplifying both treated isolated DNA strands. Amplification occurs in a manner that investigates the methylation status of both DNA strands. Detection of amplification products may be indicative of the methylation status of the gene, in particular the SYNE1 gene. The methods may be useful diagnostically, for example to indicate cancer in a sample. Various kits, primers and probes are also provided.
PCT/EP2011/056797 2010-04-30 2011-04-28 Methods for detecting epigenetic modifications WO2011135058A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32998210P 2010-04-30 2010-04-30
US61/329,982 2010-04-30

Publications (2)

Publication Number Publication Date
WO2011135058A2 WO2011135058A2 (en) 2011-11-03
WO2011135058A3 true WO2011135058A3 (en) 2012-09-13

Family

ID=44279944

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2011/056797 WO2011135058A2 (en) 2010-04-30 2011-04-28 Methods for detecting epigenetic modifications

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WO (1) WO2011135058A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104062334A (en) * 2014-07-11 2014-09-24 福州大学 Quantitative analysis method aiming at DNA methylation monitoring
CN111212918A (en) * 2017-09-15 2020-05-29 临床基因组学私人有限公司 Methylation analysis method
TWI791347B (en) * 2020-12-11 2023-02-01 酷氏基因生物科技股份有限公司 A predictive methoh for assessing the success of embryo implantation
CN113552191B (en) * 2021-07-28 2023-11-21 江苏师范大学 Construction method of proportional electrochemical sensor for detecting methylated DNA based on multilayer DNA amplification loop
CN113667726A (en) * 2021-08-31 2021-11-19 重庆医科大学国际体外诊断研究院 DNAzyme and three-way junction-mediated isothermal amplification reaction for detecting site-specific m6A

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002077272A2 (en) * 2001-03-26 2002-10-03 Epigenomics Ag Methods and nucleic acids for the analysis of hematopoietic cell proliferative disorders
WO2008100913A2 (en) * 2007-02-12 2008-08-21 The Johns Hopkins University Early detection and prognosis of colon cancers
WO2010089538A2 (en) * 2009-02-03 2010-08-12 Oncomethylome Sciences Sa Methods of detecting colorectal cancer

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4437975A (en) 1977-07-20 1984-03-20 Mobil Oil Corporation Manufacture of lube base stock oil
CA1340843C (en) 1987-07-31 1999-12-07 J. Lawrence Burg Selective amplification of target polynucleotide sequences
BR8907830A (en) 1988-12-16 1991-10-22 Siska Diagnostics Inc PROCESS FOR PREPARING A DOUBLE-FILAMENT DNA THAT CODES A SEQUENCE THAT MATCHES A OBJECTIVE NUCLEIC ACID SEQUENCE AND PROCESS USED FOR DETECTION OF AT LEAST ONE SPECIFIC OBJECTIVE NUCLEIC ACID SEQUENCE
US6017704A (en) 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
WO2004067726A2 (en) 2003-01-29 2004-08-12 Keck Graduate Institute Isothermal reactions for the amplification of oligonucleotides
EP1871912B1 (en) 2005-04-15 2012-02-29 Epigenomics AG Method for determining DNA methylation in blood or urine samples
US20070141582A1 (en) 2005-12-15 2007-06-21 Weiwei Li Method and kit for detection of early cancer or pre-cancer using blood and body fluids
GB0700374D0 (en) 2007-01-09 2007-02-14 Oncomethylome Sciences S A NDRG family methylation markers

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002077272A2 (en) * 2001-03-26 2002-10-03 Epigenomics Ag Methods and nucleic acids for the analysis of hematopoietic cell proliferative disorders
WO2008100913A2 (en) * 2007-02-12 2008-08-21 The Johns Hopkins University Early detection and prognosis of colon cancers
WO2010089538A2 (en) * 2009-02-03 2010-08-12 Oncomethylome Sciences Sa Methods of detecting colorectal cancer

Also Published As

Publication number Publication date
WO2011135058A2 (en) 2011-11-03

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