HK1248286A1 - 血浆dna的单分子测序 - Google Patents

血浆dna的单分子测序 Download PDF

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Publication number
HK1248286A1
HK1248286A1 HK18108082.2A HK18108082A HK1248286A1 HK 1248286 A1 HK1248286 A1 HK 1248286A1 HK 18108082 A HK18108082 A HK 18108082A HK 1248286 A1 HK1248286 A1 HK 1248286A1
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Hong Kong
Prior art keywords
dna fragments
concentration
dna
biological sample
plasma dna
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HK18108082.2A
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English (en)
Chinese (zh)
Inventor
Yuk-Ming Dennis Lo
Rossa Wai Kwun Chiu
Suk Hang Cheng
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The Chinese University Of Hong Kong
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Publication of HK1248286A1 publication Critical patent/HK1248286A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/197—Modifications characterised by incorporating a spacer/coupling moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/146—Concentration of target or template
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nanotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
HK18108082.2A 2015-08-12 2016-08-12 血浆dna的单分子测序 HK1248286A1 (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562204396P 2015-08-12 2015-08-12
US62/204,396 2015-08-12
PCT/CN2016/094802 WO2017025059A1 (en) 2015-08-12 2016-08-12 Single-molecule sequencing of plasma dna

Publications (1)

Publication Number Publication Date
HK1248286A1 true HK1248286A1 (zh) 2018-10-12

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US (2) US11319586B2 (enExample)
EP (1) EP3334843A4 (enExample)
JP (3) JP6867045B2 (enExample)
CN (2) CN115433769A (enExample)
AU (3) AU2016305103C1 (enExample)
CA (1) CA2995422A1 (enExample)
HK (1) HK1248286A1 (enExample)
IL (3) IL285795B (enExample)
MY (1) MY188348A (enExample)
SA (1) SA518390907B1 (enExample)
TW (2) TWI728994B (enExample)
WO (1) WO2017025059A1 (enExample)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7084470B2 (ja) 2017-08-23 2022-06-14 エフ．ホフマン－ラ ロシュ アーゲー 酵素のスクリーニング法
KR20200060410A (ko) * 2017-09-07 2020-05-29 쿠퍼제노믹스, 인크. 비침습적 착상전 유전자 진단을 위한 방법과 시스템 (systems and methods for non-invasive preimplantation genetic diagnosis)
WO2019084158A1 (en) 2017-10-26 2019-05-02 Ultima Genomics, Inc. METHODS AND SYSTEMS FOR SEQUENCE CALL
EP3731959B1 (en) 2017-12-29 2025-10-08 Clear Labs, Inc. Automated priming and library loading device
WO2019204720A1 (en) * 2018-04-20 2019-10-24 The Regents Of The University Of California Nucleic acid sequencing methods and computer-readable media for practicing same
WO2020185790A1 (en) 2019-03-10 2020-09-17 Ultima Genomics, Inc. Methods and systems for sequence calling
CN114303202A (zh) 2019-06-21 2022-04-08 酷博尔外科器械有限公司 用于确定胚胎中遗传模式的系统和方法
WO2021108532A2 (en) * 2019-11-25 2021-06-03 William Marsh Rice University Linear dna assembly for nanopore sequencing
US20230249178A1 (en) 2020-07-08 2023-08-10 Roche Sequencing Solutions, Inc. Split-pool synthesis apparatus and methods of performing split-pool synthesis
WO2022081741A1 (en) 2020-10-15 2022-04-21 Roche Sequencing Solutions, Inc. Electrophoretic devices and methods for next-generation sequencing library preparation
CN114507708A (zh) * 2020-11-16 2022-05-17 唐纳德·李 一种制备长分子测序dna的方法
WO2022194764A1 (en) 2021-03-15 2022-09-22 F. Hoffmann-La Roche Ag Targeted next-generation sequencing via anchored primer extension
WO2022200485A1 (en) 2021-03-26 2022-09-29 F. Hoffmann-La Roche Ag Hybridization buffer formulations
EP4314337A1 (en) 2021-04-01 2024-02-07 F. Hoffmann-La Roche AG Immune cell counting of sars-cov-2 patients based on immune repertoire sequencing
IL307398A (en) * 2021-04-12 2023-12-01 Univ Hong Kong Chinese Base change analysis using electrical signals
WO2023023561A1 (en) * 2021-08-17 2023-02-23 Quantapore, Inc. Multiplex binding assays with optical nanopore readout
US20250243536A1 (en) * 2021-10-18 2025-07-31 Belgian Volition Srl Use of nanopore sequencing for determining the origin of circulating dna
EP4547839A1 (en) 2022-06-30 2025-05-07 Kapa Biosystems, Inc. Controlling for tagmentation sequencing library insert size using archaeal histone-like proteins
CN114898802B (zh) * 2022-07-14 2022-09-30 臻和(北京)生物科技有限公司 基于血浆游离dna甲基化测序数据的末端序列频率分布特征确定方法、评价方法及装置
WO2024046992A1 (en) 2022-09-02 2024-03-07 F. Hoffmann-La Roche Ag Improvements to next-generation target enrichment performance
JP2026519344A (ja) 2023-04-13 2026-06-16 ベンタナ メディカル システムズ， インコーポレイテッド 固定固形腫瘍に対する増殖アッセイ
JP2026524816A (ja) 2023-06-14 2026-07-24 エフ． ホフマン－ラ ロシュ アーゲー 加速された５－ホルミルシトシンのマーキングおよび核酸メチル化シーケンシングにおける使用
WO2025193604A1 (en) 2024-03-11 2025-09-18 Ventana Medical Systems, Inc. Non-enzymatic dissociation of ffpe tissue and generation of single cells with intact cell surface markers
WO2025210056A1 (en) 2024-04-03 2025-10-09 Roche Sequencing Solutions, Inc. In vitro amplification of dna methylation patterns
WO2025247632A1 (en) 2024-05-27 2025-12-04 European Molecular Biology Laboratory Preparation of cell-free fragmented nucleic acids for genetic analysis sequencing
WO2026082489A1 (en) 2024-10-18 2026-04-23 F. Hoffmann-La Roche Ag Universal pcr to boost molecule recovery in cell free dna library preparation and target enrichment workflows
WO2026087220A1 (en) 2024-10-22 2026-04-30 F. Hoffmann-La Roche Ag Preparation of dna libraries for next generation sequencing using y-adapters modified with phosphorothioate bonds
WO2026093331A1 (en) 2024-10-28 2026-05-07 F. Hoffmann-La Roche Ag Hairpin adapter constructs and primers to generate next-generation sequencing library molecules
WO2026099278A1 (en) 2024-11-08 2026-05-15 F. Hoffmann-La Roche Ag Normalizing and pooling of pcr-free sequencing libraries
WO2026151663A1 (en) 2025-01-07 2026-07-16 Kapa Biosystems, Inc. Selective rna tagging methods of total nucleic samples for sequencing applications

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
GB0504774D0 (en) * 2005-03-08 2005-04-13 Lingvitae As Method
JP2008161056A (ja) 2005-04-08 2008-07-17 Hiroaki Mita Ｄｎａ配列解析装置、ｄｎａ配列解析方法およびプログラム
US12054776B2 (en) 2007-07-23 2024-08-06 The Chinese University Of Hong Kong Diagnosing fetal chromosomal aneuploidy using massively parallel genomic sequencing
AU2009325069B2 (en) 2008-12-11 2015-03-19 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
DE102008061772A1 (de) * 2008-12-11 2010-06-17 Febit Holding Gmbh Verfahren zur Untersuchung von Nukleinsäure-Populationen
LT3241914T (lt) 2009-11-05 2019-04-25 The Chinese University Of Hong Kong Vaisiaus genomo analizė iš motinos biologinio mėginio
CA2780016C (en) 2009-11-06 2017-09-19 The Chinese University Of Hong Kong Size-based genomic analysis
US9121823B2 (en) * 2010-02-19 2015-09-01 The Trustees Of The University Of Pennsylvania High-resolution analysis devices and related methods
KR20120045922A (ko) * 2010-11-01 2012-05-09 엘지전자 주식회사 나노 포어를 포함하는 염기 서열 결정 장치
KR102040307B1 (ko) 2010-11-30 2019-11-27 더 차이니즈 유니버시티 오브 홍콩 암과 연관된 유전적 또는 분자적 이상들의 검출
KR20140050067A (ko) * 2011-07-25 2014-04-28 옥스포드 나노포어 테크놀로지즈 리미티드 막횡단 포어를 사용한 이중 가닥 폴리뉴클레오티드 서열분석을 위한 헤어핀 루프 방법
GB2485635B (en) * 2011-07-26 2012-11-28 Verinata Health Inc Method for determining the presence or absence of different aneuploidies in a sample
US20130040827A1 (en) * 2011-08-14 2013-02-14 Stephen C. Macevicz Method and compositions for detecting and sequencing nucleic acids
CA2861457A1 (en) 2012-01-20 2013-07-25 Genia Technologies, Inc. Nanopore based molecular detection and sequencing
GB2559073A (en) * 2012-06-08 2018-07-25 Pacific Biosciences California Inc Modified base detection with nanopore sequencing
JP2015525077A (ja) * 2012-06-15 2015-09-03 ジェニア・テクノロジーズ・インコーポレイテッド チップの構成および高精度な核酸配列決定
US11261494B2 (en) * 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
GB201215449D0 (en) 2012-08-30 2012-10-17 Zoragen Biotechnologies Llp Method of detecting chromosonal abnormalities
HUE053822T2 (hu) 2012-09-20 2021-07-28 Univ Hong Kong Chinese Nem invazív módszer daganat metilom meghatározása plazmából
IL274464B2 (en) * 2013-12-11 2023-08-01 Accuragen Holdings Ltd Preparations and methods for the detection of rare sequence variants
WO2016077313A1 (en) * 2014-11-11 2016-05-19 Bgi Shenzhen Multi-pass sequencing
AU2016406443A1 (en) 2016-05-10 2018-11-15 Nec Corporation Demand and supply adjustment system, control apparatus, control method, and program

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CA2995422A1 (en) 2017-02-16
EP3334843A1 (en) 2018-06-20
AU2020202153A1 (en) 2020-04-16
JP7810455B2 (ja) 2026-02-03
CN107849607B (zh) 2022-10-11
AU2020202153B2 (en) 2023-02-02
JP7506408B2 (ja) 2024-06-26
TWI793586B (zh) 2023-02-21
WO2017025059A1 (en) 2017-02-16
IL285795B (en) 2022-07-01
IL257074A (en) 2018-03-29
IL294192B2 (en) 2023-08-01
AU2016305103B2 (en) 2020-01-02
US20170044606A1 (en) 2017-02-16
JP6867045B2 (ja) 2021-04-28
AU2023202572A1 (en) 2023-05-18
SA518390907B1 (ar) 2021-09-08
MY188348A (en) 2021-12-02
EP3334843A4 (en) 2019-01-02
IL294192A (en) 2022-08-01
JP2018531583A (ja) 2018-11-01
TW201718874A (zh) 2017-06-01
IL294192B1 (en) 2023-04-01
CN107849607A (zh) 2018-03-27
TWI728994B (zh) 2021-06-01
IL257074B (en) 2021-09-30
US11319586B2 (en) 2022-05-03
CN115433769A (zh) 2022-12-06
HK1245850A1 (zh) 2018-08-31
US20220205038A1 (en) 2022-06-30
JP2021118691A (ja) 2021-08-12
AU2023202572B2 (en) 2025-04-10
AU2016305103C1 (en) 2020-06-04
IL285795A (en) 2021-09-30
AU2016305103A1 (en) 2018-03-22
TW202130815A (zh) 2021-08-16
JP2024113130A (ja) 2024-08-21

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