CN115433769A - 血浆dna的单分子测序 - Google Patents

血浆dna的单分子测序 Download PDF

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Publication number
CN115433769A
CN115433769A CN202211147693.8A CN202211147693A CN115433769A CN 115433769 A CN115433769 A CN 115433769A CN 202211147693 A CN202211147693 A CN 202211147693A CN 115433769 A CN115433769 A CN 115433769A
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dna
sequencing
dna fragments
nanopore
plasma
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Chinese (zh)
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卢煜明
赵慧君
郑淑恒
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Chinese University of Hong Kong CUHK
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • C—CHEMISTRY; METALLURGY
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/197—Modifications characterised by incorporating a spacer/coupling moiety
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search

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  • Bioinformatics & Computational Biology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
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  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nanotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
CN202211147693.8A 2015-08-12 2016-08-12 血浆dna的单分子测序 Pending CN115433769A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201562204396P 2015-08-12 2015-08-12
US62/204,396 2015-08-12
PCT/CN2016/094802 WO2017025059A1 (en) 2015-08-12 2016-08-12 Single-molecule sequencing of plasma dna
CN201680046621.0A CN107849607B (zh) 2015-08-12 2016-08-12 血浆dna的单分子测序

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EP (1) EP3334843A4 (enExample)
JP (3) JP6867045B2 (enExample)
CN (2) CN115433769A (enExample)
AU (3) AU2016305103C1 (enExample)
CA (1) CA2995422A1 (enExample)
HK (1) HK1248286A1 (enExample)
IL (3) IL285795B (enExample)
MY (1) MY188348A (enExample)
SA (1) SA518390907B1 (enExample)
TW (2) TWI793586B (enExample)
WO (1) WO2017025059A1 (enExample)

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JP7333838B2 (ja) 2019-06-21 2023-08-25 クーパーサージカル・インコーポレイテッド 胚における遺伝パターンを決定するためのシステム、コンピュータプログラム及び方法
CN115315513A (zh) * 2019-11-25 2022-11-08 威廉马歇莱思大学 用于纳米孔测序的线性dna组装
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CN114898802B (zh) * 2022-07-14 2022-09-30 臻和(北京)生物科技有限公司 基于血浆游离dna甲基化测序数据的末端序列频率分布特征确定方法、评价方法及装置
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TW202130815A (zh) 2021-08-16
CN107849607B (zh) 2022-10-11
IL294192B1 (en) 2023-04-01
AU2023202572A1 (en) 2023-05-18
EP3334843A4 (en) 2019-01-02
TW201718874A (zh) 2017-06-01
US20220205038A1 (en) 2022-06-30
MY188348A (en) 2021-12-02
IL294192B2 (en) 2023-08-01
TWI728994B (zh) 2021-06-01
TWI793586B (zh) 2023-02-21
IL294192A (en) 2022-08-01
JP7506408B2 (ja) 2024-06-26
HK1245850A1 (zh) 2018-08-31
JP2021118691A (ja) 2021-08-12
JP2018531583A (ja) 2018-11-01
AU2016305103C1 (en) 2020-06-04
AU2016305103A1 (en) 2018-03-22
IL285795A (en) 2021-09-30
SA518390907B1 (ar) 2021-09-08
AU2020202153A1 (en) 2020-04-16
AU2023202572B2 (en) 2025-04-10
AU2016305103B2 (en) 2020-01-02
US11319586B2 (en) 2022-05-03
JP2024113130A (ja) 2024-08-21
IL257074B (en) 2021-09-30
CA2995422A1 (en) 2017-02-16
US20170044606A1 (en) 2017-02-16
CN107849607A (zh) 2018-03-27
JP6867045B2 (ja) 2021-04-28
EP3334843A1 (en) 2018-06-20
HK1248286A1 (zh) 2018-10-12
AU2020202153B2 (en) 2023-02-02
IL285795B (en) 2022-07-01
WO2017025059A1 (en) 2017-02-16
IL257074A (en) 2018-03-29

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