TWI728994B - 血漿dna之單分子定序 - Google Patents

血漿dna之單分子定序 Download PDF

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Publication number
TWI728994B
TWI728994B TW105125873A TW105125873A TWI728994B TW I728994 B TWI728994 B TW I728994B TW 105125873 A TW105125873 A TW 105125873A TW 105125873 A TW105125873 A TW 105125873A TW I728994 B TWI728994 B TW I728994B
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dna fragments
plasma
sequence
dna
serum
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TW105125873A
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Chinese (zh)
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TW201718874A (zh
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煜明 盧
慧君 趙
淑恒 鄭
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香港中文大學
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    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/197—Modifications characterised by incorporating a spacer/coupling moiety
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    • C12Q2527/00—Reactions demanding special reaction conditions
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

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  • Proteomics, Peptides & Aminoacids (AREA)
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  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
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  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Computational Biology (AREA)
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  • Evolutionary Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nanotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
TW105125873A 2015-08-12 2016-08-12 血漿dna之單分子定序 TWI728994B (zh)

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US201562204396P 2015-08-12 2015-08-12
US62/204,396 2015-08-12

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TW201718874A TW201718874A (zh) 2017-06-01
TWI728994B true TWI728994B (zh) 2021-06-01

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US (2) US11319586B2 (enExample)
EP (1) EP3334843A4 (enExample)
JP (3) JP6867045B2 (enExample)
CN (2) CN115433769A (enExample)
AU (3) AU2016305103C1 (enExample)
CA (1) CA2995422A1 (enExample)
HK (1) HK1248286A1 (enExample)
IL (3) IL285795B (enExample)
MY (1) MY188348A (enExample)
SA (1) SA518390907B1 (enExample)
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WO (1) WO2017025059A1 (enExample)

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JP2026524816A (ja) 2023-06-14 2026-07-24 エフ. ホフマン-ラ ロシュ アーゲー 加速された5-ホルミルシトシンのマーキングおよび核酸メチル化シーケンシングにおける使用
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CA2995422A1 (en) 2017-02-16
EP3334843A1 (en) 2018-06-20
AU2020202153A1 (en) 2020-04-16
JP7810455B2 (ja) 2026-02-03
CN107849607B (zh) 2022-10-11
AU2020202153B2 (en) 2023-02-02
JP7506408B2 (ja) 2024-06-26
TWI793586B (zh) 2023-02-21
WO2017025059A1 (en) 2017-02-16
IL285795B (en) 2022-07-01
IL257074A (en) 2018-03-29
IL294192B2 (en) 2023-08-01
AU2016305103B2 (en) 2020-01-02
US20170044606A1 (en) 2017-02-16
JP6867045B2 (ja) 2021-04-28
AU2023202572A1 (en) 2023-05-18
SA518390907B1 (ar) 2021-09-08
MY188348A (en) 2021-12-02
EP3334843A4 (en) 2019-01-02
IL294192A (en) 2022-08-01
JP2018531583A (ja) 2018-11-01
TW201718874A (zh) 2017-06-01
IL294192B1 (en) 2023-04-01
CN107849607A (zh) 2018-03-27
IL257074B (en) 2021-09-30
US11319586B2 (en) 2022-05-03
CN115433769A (zh) 2022-12-06
HK1245850A1 (zh) 2018-08-31
US20220205038A1 (en) 2022-06-30
JP2021118691A (ja) 2021-08-12
HK1248286A1 (zh) 2018-10-12
AU2023202572B2 (en) 2025-04-10
AU2016305103C1 (en) 2020-06-04
IL285795A (en) 2021-09-30
AU2016305103A1 (en) 2018-03-22
TW202130815A (zh) 2021-08-16
JP2024113130A (ja) 2024-08-21

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