HK1178211A1 - 甲基化高通量檢測方法 - Google Patents

甲基化高通量檢測方法

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Publication number
HK1178211A1
HK1178211A1 HK13104998.9A HK13104998A HK1178211A1 HK 1178211 A1 HK1178211 A1 HK 1178211A1 HK 13104998 A HK13104998 A HK 13104998A HK 1178211 A1 HK1178211 A1 HK 1178211A1
Authority
HK
Hong Kong
Prior art keywords
detection method
high throughput
methylation detection
throughput methylation
throughput
Prior art date
Application number
HK13104998.9A
Other languages
English (en)
Inventor
羅慧娟吉冠玉蔣慧吳明枝孫繼華吳仁花王君文高飛
Original Assignee
深圳華大基因科技服務有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 深圳華大基因科技服務有限公司 filed Critical 深圳華大基因科技服務有限公司
Publication of HK1178211A1 publication Critical patent/HK1178211A1/zh

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK13104998.9A 2011-05-23 2013-04-24 甲基化高通量檢測方法 HK1178211A1 (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201110133858.1A CN102796808B (zh) 2011-05-23 2011-05-23 甲基化高通量检测方法

Publications (1)

Publication Number Publication Date
HK1178211A1 true HK1178211A1 (zh) 2013-09-06

Family

ID=47196105

Family Applications (1)

Application Number Title Priority Date Filing Date
HK13104998.9A HK1178211A1 (zh) 2011-05-23 2013-04-24 甲基化高通量檢測方法

Country Status (6)

Country Link
US (1) US9133513B2 (zh)
EP (1) EP2725125B1 (zh)
CN (1) CN102796808B (zh)
DK (1) DK2725125T3 (zh)
HK (1) HK1178211A1 (zh)
WO (1) WO2012159564A1 (zh)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
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WO2014149356A1 (en) * 2013-03-15 2014-09-25 Abbott Molecular Inc. Detection of bisulfite converted nucleotide sequences
CN104450872A (zh) * 2013-09-25 2015-03-25 上海市肿瘤研究所 一种高通量多样本多靶点单碱基分辨率的甲基化水平检测方法
CN105400776B (zh) * 2014-09-12 2019-12-31 深圳华大智造科技有限公司 寡核苷酸接头及其在构建核酸测序单链环状文库中的应用
CN104611453B (zh) * 2015-02-28 2016-08-24 基因科技（上海）有限公司 诊断基因甲基化的探针组及其应用
WO2016160965A1 (en) * 2015-03-30 2016-10-06 Rubicon Genomics, Inc. Methods and compositions for repair of dna ends by multiple enzymatic activities
CN104894233B (zh) * 2015-04-22 2018-04-27 上海昂朴生物科技有限公司 一种多样本多片段dna甲基化高通量测序方法
CN105002567B (zh) * 2015-06-30 2017-10-13 北京百迈客生物科技有限公司 无参考基因组高通量简化甲基化测序文库的构建方法
CN105332063B (zh) * 2015-08-13 2017-04-12 厦门飞朔生物技术有限公司 一种单管高通量测序文库的构建方法
CN105506109A (zh) * 2015-12-31 2016-04-20 安诺优达基因科技(北京)有限公司 一种单细胞简化代表性重亚硫酸氢盐测序的方法及试剂盒
CN105462960B (zh) * 2016-01-08 2019-02-12 杭州千基生物科技有限公司 一种dna亚硫酸盐转化及纯化的方法
CN105925562A (zh) * 2016-05-10 2016-09-07 广州嘉检医学检测有限公司 一种富集4000人类致病靶基因的方法及试剂盒
CN108504651B (zh) * 2017-02-27 2020-09-08 深圳乐土生物科技有限公司 基于高通量测序的pcr产物大样本量混合建库的文库构建方法和试剂
CN108728903A (zh) * 2017-04-21 2018-11-02 深圳市乐土精准医疗科技有限公司 基于高通量测序用于地中海贫血大样本筛查的建库方法
CN107451419B (zh) * 2017-07-14 2020-01-24 浙江大学 通过计算机程序模拟产生简化dna甲基化测序数据的方法
CN107475779A (zh) * 2017-09-22 2017-12-15 上海美吉医学检验有限公司 适用于单细胞rrbs测序的文库建立方法及其应用
CN107937985A (zh) * 2017-10-25 2018-04-20 人和未来生物科技（长沙）有限公司 一种微量碎片化dna甲基化检测文库的构建方法和检测方法
CN108300766A (zh) * 2018-01-16 2018-07-20 四川大学 利用转座酶对染色质开放区和线粒体甲基化研究的方法
CN113166809B (zh) * 2018-12-29 2023-12-26 深圳华大生命科学研究院 一种dna甲基化检测的方法、试剂盒、装置和应用
CN109609613B (zh) * 2019-01-25 2022-03-11 艾吉泰康生物科技(北京)有限公司 一种dna羟甲基化目标区域捕获测序方法
CN113811618B (zh) * 2019-05-21 2024-02-09 深圳华大智造科技股份有限公司 基于甲基化dna目标区域构建测序文库及系统和应用
CN112553298B (zh) * 2019-09-10 2023-06-23 深圳华大生命科学研究院 一种可以改善全基因组甲基化测序偏向性的建库方法
CN110878298B (zh) * 2019-11-22 2023-09-15 深圳市易基因科技有限公司 rRNA捕获探针及其应用
CN111020017A (zh) * 2019-12-31 2020-04-17 北京优迅医学检验实验室有限公司 Dna甲基化转化效率的评估方法及其应用
CN112662760A (zh) * 2020-02-25 2021-04-16 博尔诚（北京）科技有限公司 一种癌症基因甲基化检测系统和在该系统在中执行的癌症体外检测方法
CN112126987A (zh) * 2020-08-19 2020-12-25 深圳思凝一云科技有限公司 一种用于甲基化扩增子测序的建库方法
CN111945232A (zh) * 2020-08-27 2020-11-17 天津诺禾医学检验所有限公司 构建rrbs文库的y型接头、试剂盒及方法
CN112226486B (zh) * 2020-09-24 2021-11-30 上海英基生物科技有限公司 基因组甲基化dna靶向富集的文库构建方法及其应用
CN112779320B (zh) * 2020-12-04 2023-07-14 深圳市易基因科技有限公司 多区域dna甲基化检测探针设计及其检测方法
CN114974417A (zh) * 2021-06-03 2022-08-30 广州燃石医学检验所有限公司 一种甲基化测序方法和装置
CN114058681A (zh) * 2021-12-01 2022-02-18 大连晶泰生物技术有限公司 一种基于目标区域捕获的甲基化突变检测方法及试剂盒
CN113948150B (zh) * 2021-12-21 2022-04-19 北京迈基诺基因科技股份有限公司 Jmml相关基因甲基化水平评估方法、模型及构建方法
CN114540497B (zh) * 2022-02-25 2024-02-27 博尔诚（北京）科技有限公司 用于膀胱癌筛查的标志物、探针组合物及其应用
WO2023192635A2 (en) * 2022-04-01 2023-10-05 Twist Bioscience Corporation Libraries for methylation analysis

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003035860A1 (en) * 2001-10-24 2003-05-01 The Trustees Of Columbia University In The City Of New York A method for gene identification based on differential dna methylation
AU2003224978A1 (en) * 2002-04-12 2003-10-27 Curators Of The University Of Missouri ECIST microarrays for dual screening of DNA hypermethylation and gene silencing
WO2005090607A1 (en) * 2004-03-08 2005-09-29 Rubicon Genomics, Inc. Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation
WO2008096146A1 (en) * 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
CN101802223A (zh) * 2007-08-15 2010-08-11 香港大学 用于高通量亚硫酸氢盐dna-测序的方法和组合物及其用途
CN102409408B (zh) 2010-09-21 2013-08-07 深圳华大基因科技服务有限公司 一种利用微量基因组dna进行全基因组甲基化位点精确检测的方法
WO2012058634A2 (en) * 2010-10-28 2012-05-03 Salk Institute For Biological Studies Epigenomic induced pluripotent stem cell signatures

Also Published As

Publication number Publication date
US20140256563A1 (en) 2014-09-11
EP2725125B1 (en) 2018-01-17
WO2012159564A1 (zh) 2012-11-29
EP2725125A4 (en) 2014-12-24
CN102796808B (zh) 2014-06-18
CN102796808A (zh) 2012-11-28
EP2725125A1 (en) 2014-04-30
US9133513B2 (en) 2015-09-15
DK2725125T3 (en) 2018-04-23

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