FR2479261A1 - Procede pour la determination d'acides gras libres - Google Patents
Procede pour la determination d'acides gras libres Download PDFInfo
- Publication number
- FR2479261A1 FR2479261A1 FR8026322A FR8026322A FR2479261A1 FR 2479261 A1 FR2479261 A1 FR 2479261A1 FR 8026322 A FR8026322 A FR 8026322A FR 8026322 A FR8026322 A FR 8026322A FR 2479261 A1 FR2479261 A1 FR 2479261A1
- Authority
- FR
- France
- Prior art keywords
- acyl
- fatty acids
- free fatty
- coa
- myokinase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000021588 free fatty acids Nutrition 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 15
- 238000006243 chemical reaction Methods 0.000 claims abstract description 27
- 102000002281 Adenylate kinase Human genes 0.000 claims abstract description 23
- 108020000543 Adenylate kinase Proteins 0.000 claims abstract description 23
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 22
- 102000005870 Coenzyme A Ligases Human genes 0.000 claims abstract description 14
- 108010011449 Long-chain-fatty-acid-CoA ligase Proteins 0.000 claims abstract description 14
- 102000004539 Acyl-CoA Oxidase Human genes 0.000 claims abstract description 11
- 108020001558 Acyl-CoA oxidase Proteins 0.000 claims abstract description 11
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 claims abstract description 11
- RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 claims abstract description 4
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 claims abstract description 3
- RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 claims description 5
- 239000005516 coenzyme A Substances 0.000 claims description 5
- 229940093530 coenzyme a Drugs 0.000 claims description 5
- KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 claims description 5
- -1 acyl coenzyme A Chemical compound 0.000 claims description 3
- 238000002835 absorbance Methods 0.000 abstract description 8
- 230000002255 enzymatic effect Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 239000005515 coenzyme Substances 0.000 abstract 1
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 15
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 10
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 7
- 210000002966 serum Anatomy 0.000 description 7
- 238000005259 measurement Methods 0.000 description 6
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 description 5
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 description 5
- 102000003960 Ligases Human genes 0.000 description 5
- 108090000364 Ligases Proteins 0.000 description 5
- 229940107700 pyruvic acid Drugs 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 102000003992 Peroxidases Human genes 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 108040007629 peroxidase activity proteins Proteins 0.000 description 3
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 2
- 108091006112 ATPases Proteins 0.000 description 2
- 102000057290 Adenosine Triphosphatases Human genes 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 125000002252 acyl group Chemical group 0.000 description 2
- 238000004737 colorimetric analysis Methods 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 235000019162 flavin adenine dinucleotide Nutrition 0.000 description 2
- 239000011714 flavin adenine dinucleotide Substances 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 241000222178 Candida tropicalis Species 0.000 description 1
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 description 1
- GHAZCVNUKKZTLG-UHFFFAOYSA-N N-ethyl-succinimide Natural products CCN1C(=O)CCC1=O GHAZCVNUKKZTLG-UHFFFAOYSA-N 0.000 description 1
- HDFGOPSGAURCEO-UHFFFAOYSA-N N-ethylmaleimide Chemical compound CCN1C(=O)C=CC1=O HDFGOPSGAURCEO-UHFFFAOYSA-N 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 102000013009 Pyruvate Kinase Human genes 0.000 description 1
- 108020005115 Pyruvate Kinase Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- LNQVTSROQXJCDD-UHFFFAOYSA-N adenosine monophosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)C(OP(O)(O)=O)C1O LNQVTSROQXJCDD-UHFFFAOYSA-N 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- VWWQXMAJTJZDQX-UYBVJOGSSA-N flavin adenine dinucleotide Chemical compound C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1CO[P@](O)(=O)O[P@@](O)(=O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C2=NC(=O)NC(=O)C2=NC2=C1C=C(C)C(C)=C2 VWWQXMAJTJZDQX-UYBVJOGSSA-N 0.000 description 1
- 229940093632 flavin-adenine dinucleotide Drugs 0.000 description 1
- IPCSVZSSVZVIGE-UHFFFAOYSA-M hexadecanoate Chemical compound CCCCCCCCCCCCCCCC([O-])=O IPCSVZSSVZVIGE-UHFFFAOYSA-M 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16029379A JPS5685300A (en) | 1979-12-12 | 1979-12-12 | Determination of free fatty acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2479261A1 true FR2479261A1 (fr) | 1981-10-02 |
| FR2479261B1 FR2479261B1 (OSRAM) | 1985-04-05 |
Family
ID=15711837
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR8026322A Granted FR2479261A1 (fr) | 1979-12-12 | 1980-12-11 | Procede pour la determination d'acides gras libres |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US4360591A (OSRAM) |
| JP (1) | JPS5685300A (OSRAM) |
| DE (1) | DE3044786C2 (OSRAM) |
| FR (1) | FR2479261A1 (OSRAM) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58124360A (ja) * | 1982-01-20 | 1983-07-23 | Tokyo Tatsuno Co Ltd | 電話受信方法 |
| JPS59208965A (ja) * | 1983-05-12 | 1984-11-27 | Nippon Telegr & Teleph Corp <Ntt> | 電話機の呼出方式 |
| JPS6046160A (ja) * | 1983-08-23 | 1985-03-12 | Nippon Resuko:Kk | 電話装置 |
| JPS6068743U (ja) * | 1983-11-05 | 1985-05-15 | ニツセイ電機株式会社 | 選択電話装置 |
| JPS60134655A (ja) * | 1983-12-23 | 1985-07-17 | Matsushita Electric Works Ltd | 自動応答装置 |
| JPS61296846A (ja) * | 1985-06-25 | 1986-12-27 | Sony Corp | 選択機能付留守番電話装置 |
| US5958714A (en) * | 1996-10-02 | 1999-09-28 | Safety Associates, Inc. | Test kits for determining at least two specific analytes in foods and other complex matrices |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0009781A1 (en) * | 1978-09-29 | 1980-04-16 | Mitsubishi Kasei Corporation | Process for the purification of long-chain acyl-coenzyme-A synthetase and the enzyme, acyl-CoA synthetase, purified thereby |
| DE2944498A1 (de) * | 1978-11-06 | 1980-05-14 | Nippon Shoji Kaisha Ltd | Methode zur bestimmung von acyl-coenzym a und reagens hierfuer |
| EP0019875A1 (en) * | 1979-05-25 | 1980-12-10 | Mitsubishi Kasei Corporation | Method for assaying fatty acids |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT986838B (it) * | 1972-05-12 | 1975-01-30 | Sclavo Inst Sieroterapeut | Complesso di reagenti per la deter minazione enzimatica del glucosio sistema glucosio ossidasi perossi dasi con metodo manuale e automati co con lettura a termine o in cinetica |
| JPS5217085A (en) * | 1975-07-30 | 1977-02-08 | Ono Pharmaceut Co Ltd | Method of quantitative determination of free fatty acids in serum usin g fatty acid activating enzymes |
| JPS5425892A (en) * | 1977-07-29 | 1979-02-27 | Wako Pure Chem Ind Ltd | Quantitative determination of hydrogen peroxide |
| JPS55108297A (en) * | 1979-02-13 | 1980-08-20 | Toyobo Co Ltd | Determining method of free fatty acid |
| DE3005379A1 (de) * | 1979-02-13 | 1980-08-14 | Toyo Boseki | Verfahren zur quantitativen analyse von freien fettsaeuren und reagens zur bestimmung von freien fettsaeuren |
| JPS5915625B2 (ja) * | 1979-03-01 | 1984-04-10 | 東洋紡績株式会社 | 新規なアシルコエンザイムａオキシダ−ゼおよびその製法 |
-
1979
- 1979-12-12 JP JP16029379A patent/JPS5685300A/ja active Granted
-
1980
- 1980-11-27 DE DE3044786A patent/DE3044786C2/de not_active Expired
- 1980-12-02 US US06/212,225 patent/US4360591A/en not_active Expired - Lifetime
- 1980-12-11 FR FR8026322A patent/FR2479261A1/fr active Granted
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0009781A1 (en) * | 1978-09-29 | 1980-04-16 | Mitsubishi Kasei Corporation | Process for the purification of long-chain acyl-coenzyme-A synthetase and the enzyme, acyl-CoA synthetase, purified thereby |
| DE2944498A1 (de) * | 1978-11-06 | 1980-05-14 | Nippon Shoji Kaisha Ltd | Methode zur bestimmung von acyl-coenzym a und reagens hierfuer |
| EP0019875A1 (en) * | 1979-05-25 | 1980-12-10 | Mitsubishi Kasei Corporation | Method for assaying fatty acids |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2479261B1 (OSRAM) | 1985-04-05 |
| JPS5685300A (en) | 1981-07-11 |
| US4360591A (en) | 1982-11-23 |
| JPS5648160B2 (OSRAM) | 1981-11-13 |
| DE3044786C2 (de) | 1984-09-13 |
| DE3044786A1 (de) | 1981-09-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ST | Notification of lapse |