FI97549B - Förfarande för framställning av främmande proteiner i Streptomycesceller - Google Patents

Förfarande för framställning av främmande proteiner i Streptomycesceller Download PDF

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Publication number
FI97549B
FI97549B FI882059A FI882059A FI97549B FI 97549 B FI97549 B FI 97549B FI 882059 A FI882059 A FI 882059A FI 882059 A FI882059 A FI 882059A FI 97549 B FI97549 B FI 97549B
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FI
Finland
Prior art keywords
gene
plasmid
tendamate
sequence
amino acid
Prior art date
Application number
FI882059A
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English (en)
Finnish (fi)
Other versions
FI882059A0 (sv
FI882059A (sv
FI97549C (sv
Inventor
Klaus-Peter Koller
Guenther Johannes Riess
Original Assignee
Hoechst Ag
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Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Publication of FI882059A0 publication Critical patent/FI882059A0/sv
Publication of FI882059A publication Critical patent/FI882059A/sv
Publication of FI97549B publication Critical patent/FI97549B/sv
Application granted granted Critical
Publication of FI97549C publication Critical patent/FI97549C/sv

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Claims (8)

1. Förfarande för framställning av fusionsprotein, kännetecknat därav, att det önskade proteinets 5 strukturgen kopplas tili 3'-ändan av den kodande strängen av en eventuellt modifierad tendamistatgen, vilken kodar i tabell I angivet tendamistat eller en modifikation därav, som fortfarande förmär reagera reversibelt med spesifika reseptorer, denna genkonstruktion uttrycks i Streptomyces-10 värdceller, och det utsöndrade fusionsproteinet isoleras ur odlingssupernatanten.
2. Förfarande enligt patentkrav 1, kännetecknat därav, att tendamistatgenen förkortats ut-gäende frän den kodande strängens 3'-ända.
3. Genkonstruktion, kännetecknad därav, att man till 3'-ändan av den däri ingäende kodande strängen av en eventuellt modifierad tendamistatgen, vilken kodar i tabell I angivet tendamistat eller en modifikation därav, som fortfarande förmär reagera reversibelt 20 med spesifika reseptorer, kopplat en strukturgen för ett annat protein.
4. Genkonstruktion enligt patentkrav 3, kännetecknad därav, att tendamistatgenen förkortats ut-gäende frän den kodande strängens 3'-ända.
5. Vektor, kännetecknad därav, att den innehäller en genkonstruktion enligt patentkrav 3 eller 4.
6. Streptomyces-cell, kännetecknad därav, att den innehäller en vektor enligt patentkrav 5.
7. Fusionsprotein, kännetecknat därav, 30 att det innehäller en N-terminal del, som utgörs av den i tabell I angivna tendamistatsekvensen eller en modifikation därav, som fortfarande förmär reagera reversibelt med specifikä receptorer.
8. Användning av ett fusionsprotein enligt patent-35 krav 7 eller som erhällits enligt patentkrav 1 eller 2, kännetecknad därav, att det sker för att fram-ställa ett eventuellt modifierat tendamistat och/eller dess fusionspartner.
FI882059A 1987-05-05 1988-05-03 Förfarande för framställning av främmande proteiner i Streptomycesceller FI97549C (sv)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3714866 1987-05-05
DE19873714866 DE3714866A1 (de) 1987-05-05 1987-05-05 Verfahren zur herstellung von fremdproteinen in streptomyceten

Publications (4)

Publication Number Publication Date
FI882059A0 FI882059A0 (sv) 1988-05-03
FI882059A FI882059A (sv) 1988-11-06
FI97549B true FI97549B (sv) 1996-09-30
FI97549C FI97549C (sv) 1997-01-10

Family

ID=6326841

Family Applications (1)

Application Number Title Priority Date Filing Date
FI882059A FI97549C (sv) 1987-05-05 1988-05-03 Förfarande för framställning av främmande proteiner i Streptomycesceller

Country Status (19)

Country Link
EP (1) EP0289936B1 (sv)
JP (1) JP2671260B2 (sv)
KR (1) KR970001235B1 (sv)
CN (1) CN1029989C (sv)
AR (1) AR241658A1 (sv)
AT (1) ATE94905T1 (sv)
AU (1) AU612144B2 (sv)
CA (1) CA1338338C (sv)
DE (2) DE3714866A1 (sv)
DK (1) DK175525B1 (sv)
ES (1) ES2045004T3 (sv)
FI (1) FI97549C (sv)
HU (1) HU204094B (sv)
IE (1) IE62522B1 (sv)
IL (1) IL86277A0 (sv)
NO (1) NO178036C (sv)
NZ (1) NZ224464A (sv)
PT (1) PT87400B (sv)
ZA (1) ZA883168B (sv)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5426036A (en) * 1987-05-05 1995-06-20 Hoechst Aktiengesellschaft Processes for the preparation of foreign proteins in streptomycetes
DE4012818A1 (de) 1990-04-21 1991-10-24 Hoechst Ag Verfahren zur herstellung von fremdproteinen in streptomyceten
ES2081826T3 (es) * 1988-11-03 1996-03-16 Hoechst Ag Procedimiento para la preparacion de un producto previo de insulina en estreptomicetos.
DE3844211A1 (de) * 1988-12-29 1990-07-05 Hoechst Ag Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung
DE3936876A1 (de) * 1989-11-06 1991-05-23 Hoechst Ag Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3331860A1 (de) * 1983-09-03 1985-03-21 Hoechst Ag, 6230 Frankfurt Verfahren zur herstellung von tendamistat
DE3418274A1 (de) * 1984-05-17 1985-11-21 Hoechst Ag, 6230 Frankfurt Signalpeptid fuer die exkretion von peptiden in streptomyceten
ATE63757T1 (de) * 1985-03-28 1991-06-15 Chiron Corp Expression durch verwendung von fusionsgenen fuer proteinproduktion.
DE3707150A1 (de) * 1987-03-06 1988-09-15 Hoechst Ag Tendamistat-derivate
ES2081826T3 (es) * 1988-11-03 1996-03-16 Hoechst Ag Procedimiento para la preparacion de un producto previo de insulina en estreptomicetos.

Also Published As

Publication number Publication date
CA1338338C (en) 1996-05-21
ES2045004T3 (es) 1994-01-16
HU204094B (en) 1991-11-28
IL86277A0 (en) 1988-11-15
DK241688A (da) 1988-11-06
PT87400A (pt) 1989-05-31
JP2671260B2 (ja) 1997-10-29
NZ224464A (en) 1990-02-26
FI882059A0 (sv) 1988-05-03
IE62522B1 (en) 1995-02-08
FI882059A (sv) 1988-11-06
EP0289936A2 (de) 1988-11-09
EP0289936A3 (en) 1989-11-23
DK175525B1 (da) 2004-11-22
AU612144B2 (en) 1991-07-04
AR241658A1 (es) 1992-10-30
ZA883168B (en) 1989-11-29
CN88102568A (zh) 1988-11-23
PT87400B (pt) 1992-09-30
JPS63301793A (ja) 1988-12-08
DK241688D0 (da) 1988-05-04
DE3714866A1 (de) 1988-11-24
NO178036B (no) 1995-10-02
AU1555988A (en) 1988-11-10
KR970001235B1 (ko) 1997-02-04
NO881942L (no) 1988-11-07
DE3884261D1 (de) 1993-10-28
KR880014102A (ko) 1988-12-22
EP0289936B1 (de) 1993-09-22
CN1029989C (zh) 1995-10-11
IE881338L (en) 1988-11-05
NO881942D0 (no) 1988-05-04
HUT46941A (en) 1988-12-28
NO178036C (no) 1996-01-10
FI97549C (sv) 1997-01-10
ATE94905T1 (de) 1993-10-15

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