ES2081826T3 - Procedimiento para la preparacion de un producto previo de insulina en estreptomicetos. - Google Patents

Procedimiento para la preparacion de un producto previo de insulina en estreptomicetos.

Info

Publication number
ES2081826T3
ES2081826T3 ES89120056T ES89120056T ES2081826T3 ES 2081826 T3 ES2081826 T3 ES 2081826T3 ES 89120056 T ES89120056 T ES 89120056T ES 89120056 T ES89120056 T ES 89120056T ES 2081826 T3 ES2081826 T3 ES 2081826T3
Authority
ES
Spain
Prior art keywords
insulin
streptomicets
procedure
preparation
lys
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89120056T
Other languages
English (en)
Inventor
Klaus-Peter Dr Koller
Gunther Johannes Dr Riess
Eugen Dr Uhlmann
Holger Dr Wallmeier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE3837273A external-priority patent/DE3837273A1/de
Priority claimed from DE19893927449 external-priority patent/DE3927449A1/de
Application filed by Hoechst AG filed Critical Hoechst AG
Application granted granted Critical
Publication of ES2081826T3 publication Critical patent/ES2081826T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Abstract

DICHO PROCEDIMIENTO CONSISTE EN (A) ACOPLAR UN GEN PARA PROINSULINA REDUCIDA SEGUN AFINIDADES Y TENDENCIAS DE LOS GENES: (B) COMBINAR LA INSULINA (B) CON LA CADENA (A) POR LYS O ASOCIACION LYS-LYS; (C) INSTALAR LA CONSTRUCCION DE GEN EN UN VECTOR DE EXPRESION; (D) TRANSFORMAR LA CONSTRUCCION DE GEN EN CELULAS ANFITRIONAS DE ESTREPTOMICINA; (E) EXPRESAR LA TRANSFORMACION EN FUSION DE PROTEINAS Y (F) SECRECIONAR LA FUSION DE PROTEINAS. LA FUSION DE PROTEINAS SE PUEDE DISOCIAR LIGERAMENTE EN PRECURSORES DE INSULINA.
ES89120056T 1988-11-03 1989-10-28 Procedimiento para la preparacion de un producto previo de insulina en estreptomicetos. Expired - Lifetime ES2081826T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3837273A DE3837273A1 (de) 1987-05-05 1988-11-03 Verfahren zur herstellung eines insulin-vorprodukts in streptomyceten
DE19893927449 DE3927449A1 (de) 1989-08-19 1989-08-19 Verfahren zur herstellung eines insulin-vorprodukts in streptomyceten

Publications (1)

Publication Number Publication Date
ES2081826T3 true ES2081826T3 (es) 1996-03-16

Family

ID=25873834

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89120056T Expired - Lifetime ES2081826T3 (es) 1988-11-03 1989-10-28 Procedimiento para la preparacion de un producto previo de insulina en estreptomicetos.

Country Status (18)

Country Link
EP (1) EP0367163B1 (es)
JP (1) JPH02219588A (es)
KR (1) KR900008040A (es)
CN (1) CN1042378A (es)
AT (1) ATE132531T1 (es)
AU (1) AU619054B2 (es)
CA (1) CA2002062A1 (es)
DE (1) DE58909556D1 (es)
DK (1) DK546889A (es)
ES (1) ES2081826T3 (es)
FI (1) FI95600C (es)
GR (1) GR3018738T3 (es)
HU (1) HU209596B (es)
IE (1) IE893530L (es)
IL (1) IL92178A (es)
NO (1) NO894363L (es)
NZ (1) NZ231222A (es)
PT (1) PT92177B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4012818A1 (de) 1990-04-21 1991-10-24 Hoechst Ag Verfahren zur herstellung von fremdproteinen in streptomyceten
DE3714866A1 (de) * 1987-05-05 1988-11-24 Hoechst Ag Verfahren zur herstellung von fremdproteinen in streptomyceten
US5426036A (en) * 1987-05-05 1995-06-20 Hoechst Aktiengesellschaft Processes for the preparation of foreign proteins in streptomycetes
KR100188800B1 (ko) 1990-09-05 1999-06-01 이센브룩, 라피세 프리프로인슐린을 인슐린으로 전환시키기 위한 효소적 방법
GB9513967D0 (en) * 1995-07-08 1995-09-06 Univ Leicester Insulin
JP5903269B2 (ja) 2008-08-07 2016-04-13 バイオコン リミテッド インスリン化合物の調製方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3418274A1 (de) * 1984-05-17 1985-11-21 Hoechst Ag, 6230 Frankfurt Signalpeptid fuer die exkretion von peptiden in streptomyceten
DK129385A (da) * 1985-03-22 1986-09-23 Novo Industri As Peptider og fremstilling deraf
DK437786D0 (da) * 1986-09-12 1986-09-12 Nordisk Gentofte Insulinprecursorer
DE3707150A1 (de) * 1987-03-06 1988-09-15 Hoechst Ag Tendamistat-derivate
DE3714866A1 (de) * 1987-05-05 1988-11-24 Hoechst Ag Verfahren zur herstellung von fremdproteinen in streptomyceten

Also Published As

Publication number Publication date
NZ231222A (en) 1991-08-27
FI95600B (fi) 1995-11-15
PT92177A (pt) 1990-05-31
IL92178A0 (en) 1990-07-26
JPH02219588A (ja) 1990-09-03
DK546889A (da) 1990-05-04
IL92178A (en) 1995-11-27
DK546889D0 (da) 1989-11-02
EP0367163B1 (de) 1996-01-03
FI895184A0 (fi) 1989-11-01
HUT53675A (en) 1990-11-28
HU895649D0 (en) 1990-01-28
AU619054B2 (en) 1992-01-16
FI95600C (fi) 1996-02-26
DE58909556D1 (de) 1996-02-15
NO894363D0 (no) 1989-11-02
AU4395089A (en) 1990-05-10
HU209596B (en) 1994-08-29
EP0367163A3 (de) 1991-03-06
GR3018738T3 (en) 1996-04-30
PT92177B (pt) 1995-06-30
CA2002062A1 (en) 1990-05-03
EP0367163A2 (de) 1990-05-09
KR900008040A (ko) 1990-06-02
ATE132531T1 (de) 1996-01-15
IE893530L (en) 1990-05-03
NO894363L (no) 1990-05-04
CN1042378A (zh) 1990-05-23

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