FI93124B - Förfarande för immobilisering av löst äggviteämne - Google Patents

Förfarande för immobilisering av löst äggviteämne Download PDF

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Publication number
FI93124B
FI93124B FI861662A FI861662A FI93124B FI 93124 B FI93124 B FI 93124B FI 861662 A FI861662 A FI 861662A FI 861662 A FI861662 A FI 861662A FI 93124 B FI93124 B FI 93124B
Authority
FI
Finland
Prior art keywords
carrier
egg white
protein
electrolyte
solution
Prior art date
Application number
FI861662A
Other languages
English (en)
Finnish (fi)
Other versions
FI861662A (sv
FI93124C (sv
FI861662A0 (sv
Inventor
Reiner Schnee
Dieter Kraemer
Hermann Plainer
Bruno Sproessler
Helmut Uhlig
Original Assignee
Roehm Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roehm Gmbh filed Critical Roehm Gmbh
Publication of FI861662A0 publication Critical patent/FI861662A0/sv
Publication of FI861662A publication Critical patent/FI861662A/sv
Publication of FI93124B publication Critical patent/FI93124B/sv
Application granted granted Critical
Publication of FI93124C publication Critical patent/FI93124C/sv

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
    • A23C9/1206—Lactose hydrolysing enzymes, e.g. lactase, beta-galactosidase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/328—Polymers on the carrier being further modified
    • B01J20/3282—Crosslinked polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C2220/00—Biochemical treatment
    • A23C2220/10—Enzymatic treatment
    • A23C2220/104—Enzymatic treatment with immobilised enzymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)

Claims (6)

1. Förfarande för immobilisering av löst äggviteämne ur en vattenlösning vid en i lösningen suspenderad fast, makroporös, i vatten olöslig bärare i närvaro av en elektrolyt och fixe-ring av äggviteämnet vid bäraren med hjälp av ett förnätning av vid bäraren adsorberat äggviteämne förorsakande koppiings-komponent, som innehäller tvä eller flera gentemot äggviteämnet reaktiva funktionella grupper, kännetecknat därav, att vattenlösningen av äggviteämnet, som uppvisar en jonstyrka pä elektrolyten av minst 0,30 mol/1, fär inverka pä den av denna vattenlösning omringade bäraren med en belast-ning, som är minst 20 mg äggviteämne per gram bärare, tills äggviteämnet adsorberats vid bäraren och att tili äggvite-ämnets vattenlösning sätts koppiingskomponenten under adsor-beringen eller därefter utan mellantorkning av bäraren.
2. Förfarande enligt patentkravet 1, kännetecknat därav, att en bärare används, som inte innehäller kovalent bundna katjoniska grupper eller som innehäller dessa högst 0,1 milliekvivalenter/g.
3. Förfarande enligt patentkravet 2, kännetecknat därav, att en bärare används med högst 5 milliekvivalenter kovalent bundna anjoniska grupper per gram torrämne.
4. Förfarande enligt patentkravet 3, kännetecknat därav, att en bärare används, som innehäller högst 0,1 milliekvivalenter kovalent bundna joniska grupper per gram torrämne .
5. Förfarande enligt nägot av patentkraven 1-4, k ä n -netecknad därav, att en bärare används, som i vatten-haltigt medium sväller högst sin dubbla volym.
6. Förfarande enligt patentkravet 5, kännetecknat därav, att bäraren används finfördelad, särskilt som pärlor.
FI861662A 1985-04-27 1986-04-21 Förfarande för immobilisering av löst äggviteämne FI93124C (sv)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3515252 1985-04-27
DE3515252A DE3515252C2 (de) 1985-04-27 1985-04-27 Verfahren zur Immobilisierung gelöster Eiweißstoffe

Publications (4)

Publication Number Publication Date
FI861662A0 FI861662A0 (sv) 1986-04-21
FI861662A FI861662A (sv) 1986-10-28
FI93124B true FI93124B (sv) 1994-11-15
FI93124C FI93124C (sv) 1995-02-27

Family

ID=6269271

Family Applications (1)

Application Number Title Priority Date Filing Date
FI861662A FI93124C (sv) 1985-04-27 1986-04-21 Förfarande för immobilisering av löst äggviteämne

Country Status (7)

Country Link
US (1) US4839419A (sv)
EP (1) EP0200107B1 (sv)
JP (1) JPH084505B2 (sv)
AT (1) ATE68525T1 (sv)
DE (2) DE3515252C2 (sv)
DK (1) DK189286A (sv)
FI (1) FI93124C (sv)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
US5179199A (en) * 1986-10-20 1993-01-12 Genzyme Corporation Protein purification
US5449759A (en) * 1987-05-16 1995-09-12 Somatogen, Inc. Hemoglobins with intersubunit desulfide bonds
GB2208649A (en) * 1987-08-17 1989-04-12 Vnii Biotekhnologii Immobilization of fungal beta -galactosidase on an inorganic carrier
DK427987A (da) * 1987-08-17 1989-02-18 Vnii Biotekhnologii Fremgangsmaade til immobilisering af fungal beta-galactosidase
US5545727A (en) * 1989-05-10 1996-08-13 Somatogen, Inc. DNA encoding fused di-alpha globins and production of pseudotetrameric hemoglobin
US5092992A (en) * 1989-06-07 1992-03-03 J. T. Baker Inc. Polyethyleneimine matrixes for affinity chromatography
US5085779A (en) * 1989-06-07 1992-02-04 J. T. Baker, Inc. Polyethyleneimine matrixes for affinity chromatography
DE3932521C1 (en) * 1989-09-29 1991-04-25 Nitra Gesellschaft Fuer Biotechnik Mbh, 2100 Hamburg, De Removing nitric oxide from enclosed atmos. - comprises culturing nitrifying bacteria in aq. suspension on membrane
DE4029374A1 (de) * 1990-09-15 1992-03-19 Roehm Gmbh Immobilisierte enzyme
US5200471A (en) * 1990-11-05 1993-04-06 Minnesota Mining And Manufacturing Company Biomolecules covalently immobilized with a high bound specific biological activity and method of preparing same
WO1993008842A1 (en) * 1991-11-08 1993-05-13 Somatogen, Inc. Hemoglobins as drug delivery agents
US5512169A (en) * 1992-12-30 1996-04-30 Dow Corning Corporation Liquid column packing materials
DE69428891T2 (de) * 1993-03-29 2002-06-27 Minnesota Mining And Mfg. Co., Saint Paul VERFAHREN ZUR VERBINDUNG VON LIGANDEN IN EINEM PORÖSEN TRÄGER (e.g.AZLAKTON) UND DESSEN ANWENDUNGEN
EP0713420B1 (en) * 1993-08-13 1997-08-06 Minnesota Mining And Manufacturing Company Cartridge filter with insoluble enzyme particulates contained thereon
US5468847A (en) * 1994-03-10 1995-11-21 Minnesota Mining And Manufacturing Company Method of isolating and purifying a biomacromolecule
US6833260B1 (en) 1999-10-08 2004-12-21 Protein Scientific, Inc. Lactose hydrolysis
US7060478B2 (en) * 2004-03-12 2006-06-13 Min-Hsiung Lee Process for preparing lysozyme
GB0425102D0 (en) * 2004-11-15 2004-12-15 Ciba Spec Chem Water Treat Ltd Polymeric compositions and methods of employing them in papermaking processes
US20070055013A1 (en) * 2005-02-21 2007-03-08 Noriho Kamiya Substrate and method of immobilizing protein
JP2009125006A (ja) * 2007-11-24 2009-06-11 National Institute Of Advanced Industrial & Technology シリカ系メソ多孔体−セルロース、ヘミセルロースの加水分解酵素複合体
US20110076778A1 (en) * 2008-05-21 2011-03-31 Daedalus Innovations Llc Solubilization of proteins in reverse micelles by extraction from a solid support
GB201002824D0 (en) * 2010-02-19 2010-04-07 Temasek Polytechnic A method of preparing a substrate for immobilization of functional substances thereon and the substrate obtained therefrom
US8263751B2 (en) 2010-11-19 2012-09-11 Daedalus Innovations Llc Method for removing a protein from a metal chelate resin

Family Cites Families (14)

* Cited by examiner, † Cited by third party
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US2129809A (en) * 1936-06-19 1938-09-13 Goodman Mfg Co Shaker conveyer
US3767531A (en) * 1972-02-14 1973-10-23 Us Agriculture Preparation of insolubilized enzymes
GB1514707A (en) * 1974-06-25 1978-06-21 Atomic Energy Authority Uk Immobilization of biologically active substances
US3983000A (en) * 1976-04-01 1976-09-28 Corning Glass Works Bonding proteins to inorganic supports
CA1093991A (en) * 1977-02-17 1981-01-20 Hideo Hirohara Enzyme immobilization with pullulan gel
GB1557944A (en) * 1978-01-30 1979-12-19 Sumitomo Chemical Co Enzyme immobilization carrier and preparation thereof
JPS5548392A (en) * 1978-02-17 1980-04-07 Toyo Jozo Co Ltd Novel immobilizing material combined with biologically active substance, its preparation, device comprising it, method, and preparation of support
US4440903A (en) * 1978-06-30 1984-04-03 Ramot University Authority For Applied Research And Industrial Development Ltd. Dihaloisocyanide derivatives of polymers for coupling nucleophiles
JPS5651984A (en) * 1979-10-02 1981-05-09 Sumitomo Chem Co Ltd Fixed lactase and its preparation
JPS56140890A (en) * 1980-04-04 1981-11-04 Sumitomo Chem Co Ltd Immobilized lactase and its preparation
JPS5736986A (en) * 1980-08-13 1982-02-27 Tanabe Seiyaku Co Ltd Immobilized aminoacylase agent and its preparation
GB2129809B (en) * 1982-10-06 1986-06-04 Novo Industri As Method for production of an immobilized enzyme preparation by means of a crosslinking agent
BE897926A (fr) * 1982-10-06 1984-04-05 Novo Industri As Procede de production d'une preparation d'enzyme immobilisee ay moyen d'un agent de reticulation, preparation d'enzyme immobilisee ainsi obtenue et utilisation de celle-ci
JPS59216586A (ja) * 1983-05-24 1984-12-06 Mitsubishi Petrochem Co Ltd 固定化酵素およびその製造法

Also Published As

Publication number Publication date
DE3681953D1 (de) 1991-11-21
EP0200107B1 (de) 1991-10-16
DE3515252A1 (de) 1986-11-06
DK189286D0 (da) 1986-04-24
EP0200107A2 (de) 1986-11-05
JPH084505B2 (ja) 1996-01-24
US4839419A (en) 1989-06-13
DE3515252C2 (de) 1994-02-24
EP0200107A3 (en) 1987-08-05
JPS61249390A (ja) 1986-11-06
FI861662A (sv) 1986-10-28
DK189286A (da) 1986-10-28
ATE68525T1 (de) 1991-11-15
FI93124C (sv) 1995-02-27
FI861662A0 (sv) 1986-04-21

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Owner name: ROEHM GMBH