FI118263B - Kaspaasiaktiivisuutta säätelevät peptidit - Google Patents
Kaspaasiaktiivisuutta säätelevät peptidit Download PDFInfo
- Publication number
- FI118263B FI118263B FI20021798A FI20021798A FI118263B FI 118263 B FI118263 B FI 118263B FI 20021798 A FI20021798 A FI 20021798A FI 20021798 A FI20021798 A FI 20021798A FI 118263 B FI118263 B FI 118263B
- Authority
- FI
- Finland
- Prior art keywords
- afp
- peptides
- apoptosis
- peptide
- cell
- Prior art date
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 124
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 74
- 230000000694 effects Effects 0.000 title abstract description 58
- 102000011727 Caspases Human genes 0.000 title abstract description 52
- 108010076667 Caspases Proteins 0.000 title abstract description 52
- 230000006907 apoptotic process Effects 0.000 claims abstract description 58
- 150000001413 amino acids Chemical class 0.000 claims abstract description 18
- 125000004122 cyclic group Chemical group 0.000 claims description 16
- 230000030833 cell death Effects 0.000 claims description 12
- 230000001413 cellular effect Effects 0.000 claims description 10
- 230000001105 regulatory effect Effects 0.000 claims description 8
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 6
- 210000004748 cultured cell Anatomy 0.000 claims description 2
- 230000002265 prevention Effects 0.000 claims description 2
- 230000002209 hydrophobic effect Effects 0.000 claims 2
- 210000003298 dental enamel Anatomy 0.000 claims 1
- 229920000642 polymer Polymers 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 108010026331 alpha-Fetoproteins Proteins 0.000 abstract description 126
- 230000004913 activation Effects 0.000 abstract description 58
- 102100030497 Cytochrome c Human genes 0.000 abstract description 46
- 108010075031 Cytochromes c Proteins 0.000 abstract description 46
- IYMAXBFPHPZYIK-BQBZGAKWSA-N Arg-Gly-Asp Chemical compound NC(N)=NCCC[C@H](N)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(O)=O IYMAXBFPHPZYIK-BQBZGAKWSA-N 0.000 abstract description 14
- 230000001640 apoptogenic effect Effects 0.000 abstract description 14
- 239000003814 drug Substances 0.000 abstract description 9
- 102000004127 Cytokines Human genes 0.000 abstract description 8
- 108090000695 Cytokines Proteins 0.000 abstract description 8
- 231100000433 cytotoxic Toxicity 0.000 abstract description 8
- 230000001472 cytotoxic effect Effects 0.000 abstract description 8
- 229940079593 drug Drugs 0.000 abstract description 8
- 108010072041 arginyl-glycyl-aspartic acid Proteins 0.000 abstract description 5
- 230000004071 biological effect Effects 0.000 abstract description 5
- 238000002512 chemotherapy Methods 0.000 abstract description 5
- 230000001404 mediated effect Effects 0.000 abstract description 5
- 108010088751 Albumins Proteins 0.000 abstract description 4
- 230000002401 inhibitory effect Effects 0.000 abstract description 4
- 230000036542 oxidative stress Effects 0.000 abstract description 4
- 208000023275 Autoimmune disease Diseases 0.000 abstract description 3
- 230000001900 immune effect Effects 0.000 abstract description 3
- 238000001959 radiotherapy Methods 0.000 abstract description 3
- 230000009385 viral infection Effects 0.000 abstract description 3
- 208000036142 Viral infection Diseases 0.000 abstract description 2
- 230000005775 apoptotic pathway Effects 0.000 abstract description 2
- 208000033065 inborn errors of immunity Diseases 0.000 abstract description 2
- 210000000056 organ Anatomy 0.000 abstract description 2
- 208000028529 primary immunodeficiency disease Diseases 0.000 abstract description 2
- 150000003384 small molecules Chemical class 0.000 abstract description 2
- 238000002054 transplantation Methods 0.000 abstract description 2
- 102100023635 Alpha-fetoprotein Human genes 0.000 abstract 3
- 102100027211 Albumin Human genes 0.000 abstract 1
- 102100031988 Tumor necrosis factor ligand superfamily member 6 Human genes 0.000 abstract 1
- 108050002568 Tumor necrosis factor ligand superfamily member 6 Proteins 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 102000013529 alpha-Fetoproteins Human genes 0.000 description 123
- 210000004027 cell Anatomy 0.000 description 64
- 238000001994 activation Methods 0.000 description 58
- 239000000284 extract Substances 0.000 description 39
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 22
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 22
- 101000848653 Homo sapiens Tripartite motif-containing protein 26 Proteins 0.000 description 21
- 102000046101 human AFP Human genes 0.000 description 21
- 102000008100 Human Serum Albumin Human genes 0.000 description 19
- 108091006905 Human Serum Albumin Proteins 0.000 description 19
- 108090000397 Caspase 3 Proteins 0.000 description 18
- 102000003952 Caspase 3 Human genes 0.000 description 18
- 206010028980 Neoplasm Diseases 0.000 description 18
- 230000001086 cytosolic effect Effects 0.000 description 15
- 230000001419 dependent effect Effects 0.000 description 13
- 108010069514 Cyclic Peptides Proteins 0.000 description 12
- 102000001189 Cyclic Peptides Human genes 0.000 description 12
- 235000001014 amino acid Nutrition 0.000 description 12
- 108010086821 DEVDase Proteins 0.000 description 11
- 238000007792 addition Methods 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 10
- 235000018102 proteins Nutrition 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 102000009027 Albumins Human genes 0.000 description 9
- 101000827785 Homo sapiens Alpha-fetoprotein Proteins 0.000 description 8
- 102000005962 receptors Human genes 0.000 description 8
- 108020003175 receptors Proteins 0.000 description 8
- 230000004663 cell proliferation Effects 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 238000011160 research Methods 0.000 description 7
- 210000004881 tumor cell Anatomy 0.000 description 7
- 108010090535 alpha-albumin Proteins 0.000 description 6
- 230000027455 binding Effects 0.000 description 6
- 210000004671 cell-free system Anatomy 0.000 description 6
- 230000006882 induction of apoptosis Effects 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 6
- 102000006495 integrins Human genes 0.000 description 6
- 108010044426 integrins Proteins 0.000 description 6
- 206010006187 Breast cancer Diseases 0.000 description 5
- 101150064015 FAS gene Proteins 0.000 description 5
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 5
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 5
- 230000033228 biological regulation Effects 0.000 description 5
- 239000007850 fluorescent dye Substances 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 238000010348 incorporation Methods 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 5
- UPSFMJHZUCSEHU-JYGUBCOQSA-N n-[(2s,3r,4r,5s,6r)-2-[(2r,3s,4r,5r,6s)-5-acetamido-4-hydroxy-2-(hydroxymethyl)-6-(4-methyl-2-oxochromen-7-yl)oxyoxan-3-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide Chemical compound CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]1[C@H](O)[C@@H](NC(C)=O)[C@H](OC=2C=C3OC(=O)C=C(C)C3=CC=2)O[C@@H]1CO UPSFMJHZUCSEHU-JYGUBCOQSA-N 0.000 description 5
- 238000006467 substitution reaction Methods 0.000 description 5
- 208000026310 Breast neoplasm Diseases 0.000 description 4
- 108010058883 acetyl-aspartyl-glutamyl-valyl-aspartyl-amino-4-methylcoumarin Proteins 0.000 description 4
- 230000000259 anti-tumor effect Effects 0.000 description 4
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 201000011510 cancer Diseases 0.000 description 4
- 238000004891 communication Methods 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 230000006698 induction Effects 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 239000003446 ligand Substances 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 3
- 102000003886 Glycoproteins Human genes 0.000 description 3
- 108090000288 Glycoproteins Proteins 0.000 description 3
- 206010062016 Immunosuppression Diseases 0.000 description 3
- 102100040247 Tumor necrosis factor Human genes 0.000 description 3
- 102100033732 Tumor necrosis factor receptor superfamily member 1A Human genes 0.000 description 3
- 101710187743 Tumor necrosis factor receptor superfamily member 1A Proteins 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 235000018417 cysteine Nutrition 0.000 description 3
- 210000000172 cytosol Anatomy 0.000 description 3
- 229940011871 estrogen Drugs 0.000 description 3
- 239000000262 estrogen Substances 0.000 description 3
- 230000001605 fetal effect Effects 0.000 description 3
- 230000014509 gene expression Effects 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000003102 growth factor Substances 0.000 description 3
- 230000009036 growth inhibition Effects 0.000 description 3
- 208000026278 immune system disease Diseases 0.000 description 3
- 230000001506 immunosuppresive effect Effects 0.000 description 3
- 230000004807 localization Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000007790 solid phase Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 2
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- 108010052832 Cytochromes Proteins 0.000 description 2
- 102000018832 Cytochromes Human genes 0.000 description 2
- 108010049207 Death Domain Receptors Proteins 0.000 description 2
- 102000009058 Death Domain Receptors Human genes 0.000 description 2
- 102000015212 Fas Ligand Protein Human genes 0.000 description 2
- 108010039471 Fas Ligand Protein Proteins 0.000 description 2
- 101000611023 Homo sapiens Tumor necrosis factor receptor superfamily member 6 Proteins 0.000 description 2
- -1 MORT Proteins 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 2
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 2
- 102100040403 Tumor necrosis factor receptor superfamily member 6 Human genes 0.000 description 2
- RJURFGZVJUQBHK-IIXSONLDSA-N actinomycin D Chemical compound C[C@H]1OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]2CCCN2C(=O)[C@@H](C(C)C)NC(=O)[C@H]1NC(=O)C1=C(N)C(=O)C(C)=C2OC(C(C)=CC=C3C(=O)N[C@@H]4C(=O)N[C@@H](C(N5CCC[C@H]5C(=O)N(C)CC(=O)N(C)[C@@H](C(C)C)C(=O)O[C@@H]4C)=O)C(C)C)=C3N=C21 RJURFGZVJUQBHK-IIXSONLDSA-N 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 238000003782 apoptosis assay Methods 0.000 description 2
- 229940114079 arachidonic acid Drugs 0.000 description 2
- 235000021342 arachidonic acid Nutrition 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000001363 autoimmune Effects 0.000 description 2
- 230000005907 cancer growth Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 230000024245 cell differentiation Effects 0.000 description 2
- 230000003833 cell viability Effects 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 2
- 230000003013 cytotoxicity Effects 0.000 description 2
- 231100000135 cytotoxicity Toxicity 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 229960005309 estradiol Drugs 0.000 description 2
- 229930182833 estradiol Natural products 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 206010073071 hepatocellular carcinoma Diseases 0.000 description 2
- 210000002865 immune cell Anatomy 0.000 description 2
- 230000003278 mimic effect Effects 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 238000010647 peptide synthesis reaction Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000005522 programmed cell death Effects 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 239000011535 reaction buffer Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000021014 regulation of cell growth Effects 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 230000001960 triggered effect Effects 0.000 description 2
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 1
- CONKBQPVFMXDOV-QHCPKHFHSA-N 6-[(5S)-5-[[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]methyl]-2-oxo-1,3-oxazolidin-3-yl]-3H-1,3-benzoxazol-2-one Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)C[C@H]1CN(C(O1)=O)C1=CC2=C(NC(O2)=O)C=C1 CONKBQPVFMXDOV-QHCPKHFHSA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 206010002198 Anaphylactic reaction Diseases 0.000 description 1
- 229940088872 Apoptosis inhibitor Drugs 0.000 description 1
- 102100021569 Apoptosis regulator Bcl-2 Human genes 0.000 description 1
- 108010089941 Apoptosomes Proteins 0.000 description 1
- FKBFDTRILNZGAI-IMJSIDKUSA-N Asp-Cys Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CS)C(O)=O FKBFDTRILNZGAI-IMJSIDKUSA-N 0.000 description 1
- 101150017888 Bcl2 gene Proteins 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 238000009010 Bradford assay Methods 0.000 description 1
- 102100029855 Caspase-3 Human genes 0.000 description 1
- 102100026548 Caspase-8 Human genes 0.000 description 1
- 108090000538 Caspase-8 Proteins 0.000 description 1
- 102000004039 Caspase-9 Human genes 0.000 description 1
- 108090000566 Caspase-9 Proteins 0.000 description 1
- PHEDXBVPIONUQT-UHFFFAOYSA-N Cocarcinogen A1 Natural products CCCCCCCCCCCCCC(=O)OC1C(C)C2(O)C3C=C(C)C(=O)C3(O)CC(CO)=CC2C2C1(OC(C)=O)C2(C)C PHEDXBVPIONUQT-UHFFFAOYSA-N 0.000 description 1
- 101000802895 Dendroaspis angusticeps Fasciculin-1 Proteins 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 101100044298 Drosophila melanogaster fand gene Proteins 0.000 description 1
- 102000007989 Effector Caspases Human genes 0.000 description 1
- 108010089510 Effector Caspases Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102100026693 FAS-associated death domain protein Human genes 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 101000793880 Homo sapiens Caspase-3 Proteins 0.000 description 1
- 101000911074 Homo sapiens FAS-associated death domain protein Proteins 0.000 description 1
- 101000958041 Homo sapiens Musculin Proteins 0.000 description 1
- 101000850748 Homo sapiens Tumor necrosis factor receptor type 1-associated DEATH domain protein Proteins 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 206010021460 Immunodeficiency syndromes Diseases 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- FGNQZXKVAZIMCI-CIUDSAMLSA-N Leu-Asp-Cys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CS)C(=O)O)N FGNQZXKVAZIMCI-CIUDSAMLSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 206010051141 Myeloblastoma Diseases 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 101100335198 Pneumocystis carinii fol1 gene Proteins 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 206010040070 Septic Shock Diseases 0.000 description 1
- 206010052779 Transplant rejections Diseases 0.000 description 1
- 108060008683 Tumor Necrosis Factor Receptor Proteins 0.000 description 1
- 102100033733 Tumor necrosis factor receptor superfamily member 1B Human genes 0.000 description 1
- 101710187830 Tumor necrosis factor receptor superfamily member 1B Proteins 0.000 description 1
- 102100033081 Tumor necrosis factor receptor type 1-associated DEATH domain protein Human genes 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 102000035181 adaptor proteins Human genes 0.000 description 1
- 108091005764 adaptor proteins Proteins 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 230000000735 allogeneic effect Effects 0.000 description 1
- 230000036783 anaphylactic response Effects 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000003302 anti-idiotype Effects 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 239000000158 apoptosis inhibitor Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 108010008124 aspartyl-glutamyl-valyl-aspartyl-7-amino-4-trifluoromethylcoumarin Proteins 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000008512 biological response Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 101150055276 ced-3 gene Proteins 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 230000036755 cellular response Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 108010045325 cyclic arginine-glycine-aspartic acid peptide Proteins 0.000 description 1
- 150000001945 cysteines Chemical class 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 229940127089 cytotoxic agent Drugs 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000006471 dimerization reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011536 extraction buffer Substances 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 230000030279 gene silencing Effects 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 102000046949 human MSC Human genes 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000001146 hypoxic effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000008073 immune recognition Effects 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000008611 intercellular interaction Effects 0.000 description 1
- 230000019189 interleukin-1 beta production Effects 0.000 description 1
- 230000006662 intracellular pathway Effects 0.000 description 1
- 230000005865 ionizing radiation Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000006510 metastatic growth Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000003607 modifier Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000017066 negative regulation of growth Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 102000006255 nuclear receptors Human genes 0.000 description 1
- 108020004017 nuclear receptors Proteins 0.000 description 1
- 239000000082 organ preservation Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000001175 peptic effect Effects 0.000 description 1
- 238000002823 phage display Methods 0.000 description 1
- PHEDXBVPIONUQT-RGYGYFBISA-N phorbol 13-acetate 12-myristate Chemical compound C([C@]1(O)C(=O)C(C)=C[C@H]1[C@@]1(O)[C@H](C)[C@H]2OC(=O)CCCCCCCCCCCCC)C(CO)=C[C@H]1[C@H]1[C@]2(OC(C)=O)C1(C)C PHEDXBVPIONUQT-RGYGYFBISA-N 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000025915 regulation of apoptotic process Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000036303 septic shock Effects 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 210000001685 thyroid gland Anatomy 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 238000011830 transgenic mouse model Methods 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 230000004565 tumor cell growth Effects 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 230000006433 tumor necrosis factor production Effects 0.000 description 1
- 102000003298 tumor necrosis factor receptor Human genes 0.000 description 1
- 210000003954 umbilical cord Anatomy 0.000 description 1
- 238000002424 x-ray crystallography Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/18—Antivirals for RNA viruses for HIV
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Virology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- AIDS & HIV (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Detergent Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (13)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI20021798A FI118263B (fi) | 2002-10-09 | 2002-10-09 | Kaspaasiaktiivisuutta säätelevät peptidit |
| PT03753618T PT1558649E (pt) | 2002-10-09 | 2003-10-07 | Péptidos que modulam a activação de caspases |
| PCT/FI2003/000735 WO2004033500A1 (fr) | 2002-10-09 | 2003-10-07 | Peptides modulant l'activation des caspases |
| US10/530,779 US7446095B2 (en) | 2002-10-09 | 2003-10-07 | Peptides modulating caspase activation |
| AT03753618T ATE470677T1 (de) | 2002-10-09 | 2003-10-07 | Peptid zur modulation der caspase-aktivierung |
| CNB2003801011658A CN100551930C (zh) | 2002-10-09 | 2003-10-07 | 调节caspase活化的肽 |
| ES03753618T ES2347234T3 (es) | 2002-10-09 | 2003-10-07 | Peptido modulador de la activacion de caspasa. |
| DE60332950T DE60332950D1 (de) | 2002-10-09 | 2003-10-07 | Peptid zur modulation der caspase-aktivierung |
| EP03753618A EP1558649B1 (fr) | 2002-10-09 | 2003-10-07 | Peptide modulant l'activation des caspases |
| DK03753618.2T DK1558649T3 (da) | 2002-10-09 | 2003-10-07 | Peptid til modulering af caspaseaktivering |
| EA200500606A EA010058B1 (ru) | 2002-10-09 | 2003-10-07 | Пептиды, модулирующие активацию каспазы |
| AU2003271784A AU2003271784A1 (en) | 2002-10-09 | 2003-10-07 | Peptides modulating caspase activation |
| JP2004542524A JP4446121B2 (ja) | 2002-10-09 | 2003-10-07 | カスパーゼ活性化調節ペプチド |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI20021798 | 2002-10-09 | ||
| FI20021798A FI118263B (fi) | 2002-10-09 | 2002-10-09 | Kaspaasiaktiivisuutta säätelevät peptidit |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI20021798A0 FI20021798A0 (fi) | 2002-10-09 |
| FI20021798A FI20021798A (fi) | 2004-04-10 |
| FI118263B true FI118263B (fi) | 2007-09-14 |
Family
ID=8564726
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI20021798A FI118263B (fi) | 2002-10-09 | 2002-10-09 | Kaspaasiaktiivisuutta säätelevät peptidit |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US7446095B2 (fr) |
| EP (1) | EP1558649B1 (fr) |
| JP (1) | JP4446121B2 (fr) |
| CN (1) | CN100551930C (fr) |
| AT (1) | ATE470677T1 (fr) |
| AU (1) | AU2003271784A1 (fr) |
| DE (1) | DE60332950D1 (fr) |
| DK (1) | DK1558649T3 (fr) |
| EA (1) | EA010058B1 (fr) |
| ES (1) | ES2347234T3 (fr) |
| FI (1) | FI118263B (fr) |
| PT (1) | PT1558649E (fr) |
| WO (1) | WO2004033500A1 (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102481369A (zh) * | 2009-06-16 | 2012-05-30 | 东海大学 | 抗革兰氏阴性菌剂 |
| CN101812448B (zh) * | 2010-01-15 | 2011-12-07 | 浙江大学 | 一种肝癌靶向双重组凋亡蛋白表达序列及应用 |
| CN102060913B (zh) * | 2010-11-30 | 2012-09-05 | 暨南大学 | 一种与人血清白蛋白结合的七肽与应用 |
| EP2970372B1 (fr) * | 2013-03-15 | 2020-09-30 | Celgene Corporation | Lymphocytes t modifiés |
| CN108186666B (zh) * | 2018-01-29 | 2020-02-21 | 西北大学 | 一种dna纳米带在抗细胞凋亡上的应用及其制备方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1226551B (it) | 1988-07-29 | 1991-01-24 | Sclavo Spa | Peptide sintetico immunologicamente attivo capace di indurre la produzione di anticorpi con elevata specificita' verso l'alfa- fetoproteina e loro impiego in campo diagnostico |
| CN1150030C (zh) * | 1995-01-24 | 2004-05-19 | 马蒂尼克斯研究与发展公司 | 重组人甲胎蛋白及其用途 |
| AU6887096A (en) | 1996-09-11 | 1998-04-02 | Patrick T. Prendergast | Immune direction therapy |
| KR100460583B1 (ko) * | 1997-02-13 | 2004-12-08 | 더 리전츠 오브 더 유니버시티 오브 캘리포니아 | 간세포 암의 예방 및 치료 방법 |
| AU7104500A (en) * | 1999-09-02 | 2001-03-26 | Atlantic Biopharmaceuticals, Inc. | Use of rafp to inhibit or prevent apoptosis |
| DE60143818D1 (de) * | 2001-06-02 | 2011-02-17 | Clf Medical Technology Acceleration Program Inc | Alpha-fetoprotein-peptide und ihre verwendung |
-
2002
- 2002-10-09 FI FI20021798A patent/FI118263B/fi active IP Right Grant
-
2003
- 2003-10-07 JP JP2004542524A patent/JP4446121B2/ja not_active Expired - Fee Related
- 2003-10-07 WO PCT/FI2003/000735 patent/WO2004033500A1/fr active Application Filing
- 2003-10-07 ES ES03753618T patent/ES2347234T3/es not_active Expired - Lifetime
- 2003-10-07 CN CNB2003801011658A patent/CN100551930C/zh not_active Expired - Fee Related
- 2003-10-07 DK DK03753618.2T patent/DK1558649T3/da active
- 2003-10-07 PT PT03753618T patent/PT1558649E/pt unknown
- 2003-10-07 EA EA200500606A patent/EA010058B1/ru not_active IP Right Cessation
- 2003-10-07 US US10/530,779 patent/US7446095B2/en not_active Expired - Fee Related
- 2003-10-07 AU AU2003271784A patent/AU2003271784A1/en not_active Abandoned
- 2003-10-07 AT AT03753618T patent/ATE470677T1/de active
- 2003-10-07 EP EP03753618A patent/EP1558649B1/fr not_active Expired - Lifetime
- 2003-10-07 DE DE60332950T patent/DE60332950D1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CN1703428A (zh) | 2005-11-30 |
| EP1558649B1 (fr) | 2010-06-09 |
| ES2347234T3 (es) | 2010-10-27 |
| EP1558649A1 (fr) | 2005-08-03 |
| DE60332950D1 (de) | 2010-07-22 |
| JP4446121B2 (ja) | 2010-04-07 |
| FI20021798A0 (fi) | 2002-10-09 |
| FI20021798A (fi) | 2004-04-10 |
| EA010058B1 (ru) | 2008-06-30 |
| US7446095B2 (en) | 2008-11-04 |
| AU2003271784A1 (en) | 2004-05-04 |
| PT1558649E (pt) | 2010-09-14 |
| CN100551930C (zh) | 2009-10-21 |
| JP2006518703A (ja) | 2006-08-17 |
| WO2004033500A1 (fr) | 2004-04-22 |
| EA200500606A1 (ru) | 2006-06-30 |
| US20060280732A1 (en) | 2006-12-14 |
| ATE470677T1 (de) | 2010-06-15 |
| DK1558649T3 (da) | 2010-10-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2020294337C1 (en) | Inhibition of cytokine-induced SH2 protein in NK cells | |
| Anderson et al. | A monoclonal antibody reactive with a 15-kDa cytoplasmic granule-associated protein defines a subpopulation of CD8+ T lymphocytes. | |
| Kirken et al. | Tyrphostin AG-490 inhibits cytokine-mediated JAK3/STAT5a/b signal transduction and cellular proliferation of antigen-activated human T cells | |
| Makarenkova et al. | CD11b+/Gr-1+ myeloid suppressor cells cause T cell dysfunction after traumatic stress | |
| KR101826460B1 (ko) | 위장암 및 위암을 비롯한 여러가지 종양에 대한 신규한 면역 요법 | |
| Lowin et al. | Comparison of Fas (Apo-1/CD95)-and perforin-mediated cytotoxicity in primary T lymphocytes | |
| Slawin et al. | Dietary fenretinide, a synthetic retinoid, decreases the tumor incidence and the tumor mass of ras+ myc-induced carcinomas in the mouse prostate reconstitution model system | |
| CA2696701C (fr) | Peptide cdca1 et agent pharmaceutique le comprenant | |
| JP6438100B2 (ja) | Ｘｂｐ１ペプチド、ｃｄ１３８ペプチドおよびｃｓ１ペプチド | |
| CA2436281A1 (fr) | Molecules chimeres contenant un module pouvant cibler des cellules specifiques et un module regulant la fonction apoptogene du complexe de permeabilite mitochondriale (ptpc) | |
| Hameed et al. | Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. | |
| US7119071B2 (en) | Amino terminal substance P compositions and methods for using the same | |
| WO2022171032A1 (fr) | Peptide épitope du mutant ras g13d et récepteur de lymphocyte t reconnaissant le mutant ras g13d | |
| FI118263B (fi) | Kaspaasiaktiivisuutta säätelevät peptidit | |
| Feng et al. | Isolation and potential immunological characterization of TPSGLVY, a novel bursal septpeptide isolated from the bursa of Fabricius | |
| Sharova | Immune proteasomes and immunity | |
| JP2009261412A (ja) | 白血球活性化ペプチド | |
| KR102017973B1 (ko) | 항-b형 간염 바이러스 x 단백질 폴리펩티드 약제 | |
| KR20010043088A (ko) | 신규인 폴리펩티드, 그 폴리펩티드를 코드화하는 ｃＤＮＡ및 그 용도 | |
| US20100215583A1 (en) | Cell nucleus-entering compositions | |
| Meng et al. | Identification of an HLA-DPB1* 0501 restricted Melan-A/MART-1 epitope recognized by CD4+ T lymphocytes: prevalence for immunotherapy in Asian populations | |
| JP2006516899A6 (ja) | 白血球活性化ペプチド | |
| KobayashiSJ et al. | The Pmel17/Silver locus protein | |
| KR20110123491A (ko) | 프로테아좀 저해제 및 쥐알파12/쥐알파13 단백질 기능의 하향 조절제를 포함하는 의약 조성물 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Patent granted |
Ref document number: 118263 Country of ref document: FI |