FI109766B - Förfarande för framställning av proteolytisk blandning innehållande escharas - Google Patents

Förfarande för framställning av proteolytisk blandning innehållande escharas Download PDF

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Publication number
FI109766B
FI109766B FI942603A FI942603A FI109766B FI 109766 B FI109766 B FI 109766B FI 942603 A FI942603 A FI 942603A FI 942603 A FI942603 A FI 942603A FI 109766 B FI109766 B FI 109766B
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FI
Finland
Prior art keywords
antioxidant
mixture
menetelmä
escarase
proteolytic
Prior art date
Application number
FI942603A
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English (en)
Finnish (fi)
Other versions
FI942603A (sv
FI942603A0 (sv
Inventor
John C Houck
Gerold K V Klein
Original Assignee
Klein Margaret L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Klein Margaret L filed Critical Klein Margaret L
Publication of FI942603A0 publication Critical patent/FI942603A0/sv
Publication of FI942603A publication Critical patent/FI942603A/sv
Application granted granted Critical
Publication of FI109766B publication Critical patent/FI109766B/sv

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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00Drugs for dermatological disorders
    • A61P17/02Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/63Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Dermatology (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (5)

1. Förfarande för framställning av en proteolytisk blandning som innehäller , escharas, kännetecknat av att man I I I ( · '* / (a) extraherar ett bromelainpreparat med en utspädd vattenhaltig antioxidant- ! » · -1 20 lösning vid ett pH-värde av 3-4, och I f (b) utfäller den önskade proteolytiska blandningen ur det i steg (a) bildad sura ex-, : traktet genom att däri tillsätta ett proteinutfällningsmedel. i »
1. Menetelmä eskaraasia sisältävän proteolyyttisen seoksen valmistamiseksi, tunnettu siitä, että (a) bromelaiinipreparaatti uutetaan laimealla vesipitoisella antioksidanttiliuoksella 5 pH-arvossa 3-4, ja (b) vaiheessa (a) muodostuneesta happamasta uutteesta saostetaan haluttu proteo-lyyttinen seos lisäämällä uutteeseen proteiinien saostinta.
2. Förfarande enligt patentkrav 1, kännetecknat av att antioxidanten är askorbin-syra. 25 3. Förfarande enligt patentkrav 1, kännetecknat av att proteinutfällningsmedlet . * är ammoniumsulfat. ♦
2. Patenttivaatimuksen 1 mukainen menetelmä, tunnettu siitä, että antioksidantti on askorbiinihappo.
3. Patenttivaatimuksen 1 mukainen menetelmä, tunnettu siitä, että proteiinien saostin on ammoniumsulfaatti.
4. Förfarande enligt patentkrav 1, kännetecknat av att antioxidanten är askorbin-syra och proteinutfällningsmedlet är ammoniumsulfat. 109766
4. Patenttivaatimuksen 1 mukainen menetelmä, tunnettu siitä, että antioksidantti on askorbiinihappo ja proteiinien saostin on ammoniumsulfaatti.
5. Jonkin edellisen patenttivaatimuksen mukainen menetelmä, tunnettu siitä, et-15 tä proteolyyttinen seos sisältää 1-1,5 % eskaraasia. , , Patentkrav I · • »
5. Förfarande enligt nägot av föregaende patentkrav, kännetecknat av att den proteolytiska blandningen innehaller 1-1,5 % escharas. f s 1 ! *
FI942603A 1991-12-03 1994-06-02 Förfarande för framställning av proteolytisk blandning innehållande escharas FI109766B (sv)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US80196891A 1991-12-03 1991-12-03
US80196891 1991-12-03
US9210395 1992-12-02
PCT/US1992/010395 WO1993010811A1 (en) 1991-12-03 1992-12-02 Proteolytic mixture containing escharase and method of isolating same

Publications (3)

Publication Number Publication Date
FI942603A0 FI942603A0 (sv) 1994-06-02
FI942603A FI942603A (sv) 1994-08-02
FI109766B true FI109766B (sv) 2002-10-15

Family

ID=25182476

Family Applications (1)

Application Number Title Priority Date Filing Date
FI942603A FI109766B (sv) 1991-12-03 1994-06-02 Förfarande för framställning av proteolytisk blandning innehållande escharas

Country Status (9)

Country Link
EP (1) EP0618811B1 (sv)
JP (1) JP3255643B2 (sv)
AU (1) AU676464B2 (sv)
CA (1) CA2125158C (sv)
DE (1) DE69230887T2 (sv)
ES (1) ES2146607T3 (sv)
FI (1) FI109766B (sv)
HU (1) HU214641B (sv)
WO (1) WO1993010811A1 (sv)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL165334A0 (en) * 2004-11-22 2006-01-15 Mediwound Ltd Debriding composition from bromelain and methods of producing same
CA3012299C (en) 2016-01-31 2022-05-31 Mediwound Ltd. Debriding composition for treating wounds

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3817834A (en) * 1970-09-02 1974-06-18 C Wilson Recovery of salted-out proteins
FR2423476A1 (fr) * 1978-04-06 1979-11-16 Oreal Nouvelles dihydroxy-2,4 diphenylamines et compositions tinctoriales pour cheveux les contenant
US4329430A (en) * 1979-06-04 1982-05-11 Klein Gerold K V Enzyme mixture
LU83177A1 (fr) * 1981-02-27 1982-09-10 Oreal Utilisation d'hydroxyanthraquinones pour la coloration des fibres keratiniques humaines,procede et composition les mettant en oeuvre

Also Published As

Publication number Publication date
EP0618811A1 (en) 1994-10-12
HU214641B (hu) 1998-04-28
AU3234293A (en) 1993-06-28
HUT71383A (en) 1995-11-28
HU9401684D0 (en) 1994-09-28
DE69230887D1 (de) 2000-05-11
CA2125158A1 (en) 1993-06-10
FI942603A (sv) 1994-08-02
ES2146607T3 (es) 2000-08-16
EP0618811A4 (en) 1996-05-29
JP3255643B2 (ja) 2002-02-12
WO1993010811A1 (en) 1993-06-10
EP0618811B1 (en) 2000-04-05
DE69230887T2 (de) 2000-10-12
JPH08508635A (ja) 1996-09-17
AU676464B2 (en) 1997-03-13
CA2125158C (en) 2007-09-18
FI942603A0 (sv) 1994-06-02

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Owner name: KLEIN, MARGARET L.

MA Patent expired