FI105482B - Menetelmä heterologisen proteiinin valmistamiseksi eukaryoottisessa isäntäsolussa - Google Patents

Menetelmä heterologisen proteiinin valmistamiseksi eukaryoottisessa isäntäsolussa Download PDF

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Publication number
FI105482B
FI105482B FI925678A FI925678A FI105482B FI 105482 B FI105482 B FI 105482B FI 925678 A FI925678 A FI 925678A FI 925678 A FI925678 A FI 925678A FI 105482 B FI105482 B FI 105482B
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FI
Finland
Prior art keywords
sequence
kex2
yeast
host cell
protein
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FI925678A
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English (en)
Finnish (fi)
Swedish (sv)
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FI925678A (fi
FI925678A0 (fi
Inventor
Bhabatosh Chaudhuri
Howard Riezman
Christine Stephan
Peter Seeboth
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Novartis Ag
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Publication of FI925678A0 publication Critical patent/FI925678A0/fi
Publication of FI925678A publication Critical patent/FI925678A/fi
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Publication of FI105482B publication Critical patent/FI105482B/fi

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/65Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/06Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/70Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
FI925678A 1991-12-16 1992-12-14 Menetelmä heterologisen proteiinin valmistamiseksi eukaryoottisessa isäntäsolussa FI105482B (fi)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP91810984 1991-12-16
EP91810984 1991-12-16

Publications (3)

Publication Number Publication Date
FI925678A0 FI925678A0 (fi) 1992-12-14
FI925678A FI925678A (fi) 1993-06-17
FI105482B true FI105482B (fi) 2000-08-31

Family

ID=8208912

Family Applications (1)

Application Number Title Priority Date Filing Date
FI925678A FI105482B (fi) 1991-12-16 1992-12-14 Menetelmä heterologisen proteiinin valmistamiseksi eukaryoottisessa isäntäsolussa

Country Status (17)

Country Link
US (2) US5501975A (el)
EP (1) EP0548012B1 (el)
JP (1) JP3305781B2 (el)
KR (1) KR100270283B1 (el)
AT (1) ATE157703T1 (el)
AU (1) AU667852B2 (el)
CA (1) CA2085308C (el)
DE (1) DE69222013T2 (el)
DK (1) DK0548012T3 (el)
ES (1) ES2107520T3 (el)
FI (1) FI105482B (el)
GR (1) GR3025324T3 (el)
IL (1) IL104075A (el)
MX (1) MX9207291A (el)
NO (1) NO308619B1 (el)
NZ (1) NZ245469A (el)
ZA (1) ZA929711B (el)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9404270D0 (en) * 1994-03-05 1994-04-20 Delta Biotechnology Ltd Yeast strains and modified albumins
NZ304538A (en) * 1995-02-27 1999-08-30 Abbott Lab Process for producing a modified recombinant protein in a host cell comprising preparing a single vector encoding both the exogenous protein and an enzyme capable of modifying the exogenous protein
CA2213734A1 (en) * 1995-02-27 1996-09-06 Abbott Laboratories A plasmid for expressing modified recombinant proteins in a bacterial system
US5942254A (en) * 1995-02-27 1999-08-24 Abbott Laboratories Phosphorylated recombinant human β-casein expressed in a bacterial system
US5710044A (en) * 1995-02-27 1998-01-20 Abbott Laboratories Plasmid for expressing phosphorylated recombinant proteins in a bacterial system
US5807702A (en) * 1995-02-27 1998-09-15 Abbott Laboratories Method for expressing phosphorylated recombinant human β-casein in a bacterial system
AT404838B (de) * 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
FR2751000B1 (fr) 1996-07-12 1998-10-30 Inst Nat Sante Rech Med Adn specifiques des bacteries de l'espece neisseria meningitidis, leurs procedes d'obtention et leurs applications biologiques
WO2000028065A1 (en) * 1998-11-06 2000-05-18 Novo Nordisk A/S Method for the production of fvii
WO2002000856A2 (en) 2000-06-30 2002-01-03 Flanders Interuniversity Institute For Biotechnology (Vib) Protein glycosylation modification in pichia pastoris
DE10221411B4 (de) * 2002-05-14 2004-07-08 GSF-Forschungszentrum für Umwelt und Gesundheit GmbH Rekombinantes Fowlpox-Virus
US20040117874A1 (en) * 2002-10-15 2004-06-17 Jianjun Yang Methods for accumulating translocated proteins
US7507573B2 (en) 2003-11-14 2009-03-24 Vib, Vzw Modification of protein glycosylation in methylotrophic yeast
JP5112064B2 (ja) * 2004-05-21 2013-01-09 エムオー バイオ ラボラトリーズ インコーポレイテッド 環境サンプルおよび生物学的サンプル中の核酸から夾雑物を除去するためのキットおよび方法
US20080082338A1 (en) * 2006-09-29 2008-04-03 O'neil Michael P Systems and methods for secure voice identification and medical device interface
RU2663587C2 (ru) 2013-03-06 2018-08-07 ГЛЭКСОСМИТКЛАЙН ЭлЭлСи Клетки-хозяева и способы использования

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4885166A (en) * 1985-06-11 1989-12-05 Ciba-Geigy Corporation Hybrid interferons
US5391485A (en) * 1985-08-06 1995-02-21 Immunex Corporation DNAs encoding analog GM-CSF molecules displaying resistance to proteases which cleave at adjacent dibasic residues
FI100106B (fi) * 1986-12-05 1997-09-30 Novartis Ag Menetelmä plasminogeenin yksisäikeisen yhdistelmäaktivaattorin valmist amiseksi
JP2643968B2 (ja) * 1988-02-03 1997-08-25 サントリー株式会社 Kex2エンドプロテアーゼ及びその製造方法

Also Published As

Publication number Publication date
CA2085308A1 (en) 1993-06-17
IL104075A (en) 2003-01-12
IL104075A0 (en) 1993-05-13
FI925678A (fi) 1993-06-17
ZA929711B (en) 1993-06-16
NO924847L (no) 1993-06-17
NZ245469A (en) 1995-03-28
EP0548012B1 (en) 1997-09-03
MX9207291A (es) 1993-07-01
DE69222013T2 (de) 1998-01-29
DE69222013D1 (de) 1997-10-09
NO924847D0 (no) 1992-12-15
KR100270283B1 (ko) 2000-11-01
US5501975A (en) 1996-03-26
AU667852B2 (en) 1996-04-18
NO308619B1 (no) 2000-10-02
US5618690A (en) 1997-04-08
AU3007192A (en) 1993-06-17
DK0548012T3 (da) 1998-04-14
FI925678A0 (fi) 1992-12-14
ES2107520T3 (es) 1997-12-01
JPH06197787A (ja) 1994-07-19
KR930013115A (ko) 1993-07-21
JP3305781B2 (ja) 2002-07-24
EP0548012A1 (en) 1993-06-23
GR3025324T3 (en) 1998-02-27
ATE157703T1 (de) 1997-09-15
CA2085308C (en) 2003-12-02

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Owner name: NOVARTIS AG

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