FI104636B - Hybridjäst, som innehåller DNA-sekvenser, som kodar för xylosreduktas- och xylitoldehydrogenasenzymer - Google Patents
Hybridjäst, som innehåller DNA-sekvenser, som kodar för xylosreduktas- och xylitoldehydrogenasenzymer Download PDFInfo
- Publication number
- FI104636B FI104636B FI924461A FI924461A FI104636B FI 104636 B FI104636 B FI 104636B FI 924461 A FI924461 A FI 924461A FI 924461 A FI924461 A FI 924461A FI 104636 B FI104636 B FI 104636B
- Authority
- FI
- Finland
- Prior art keywords
- xylose
- xylitol
- transformed
- yeast
- yeast strain
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Detergent Compositions (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Claims (11)
1. Förfarande för framställning av xylitol, käiinetecknat därav, att man a) odlar i ett xyloshaltigt medium en jäststam transformerad med en DNA- 5 sekvens som kodar föt ett xylosreduktasenzym, vilken DNA-sekvens dä den transformerats i den nämnda jäststammen ger jäststammen förmäga att reduce-ra xylos tili xylitol, och b) ätervinner bildad xylitol frän mediet. 10
2. Förfarande enligt patentkravet 1, kännetecknat därav, att den nämnda DNA- sekvensen kodar för en polypeptid omfattande väsentligen aminosyrasekvensen enligt SEKV.ID-nr 2, eller ett fragment därav, väri den nämnda polypeptiden eller fragmentet uppvisar xylosreduktasaktivitet. 15
3. Förfarande enligt patentkravet 1 eller 2, kännetecknat därav, att den nämnda transformerade jäststammen tillhör arten Saccharomyces cerevisiae, Kluyveromy-ces spp., Schizosaccharomyces pombe eller Pichia spp.
4. Förfarande enligt nägot av patentkraven 1-3, kännetecknat därav, att den nämn-20 da transformerade jäststammen tillhör arten Saccharomyces cerevisiae.
5. Förfarande enligt nägot av patentkraven 1-4, kännetecknat därav, att den nämn-da transformerade jäststammen är Saccharomyces cerevisiae H475 eller H477, som erhälles genom transformation av Saccharomyces cerevisiae GPY55-15B<% 25 eller respektive S150-2B med plasmiden pUA103, som anges i fig. 1.
6. Förfarande enligt patentkravet 1, kännetecknat därav, att minst en ytterligare ‘ ' kolkalla finns närvarande i mediet för att öka regenereringen av en ko-faktor, som är NADPH eller NADH, som krävs för xylosreduktasaktivitet.
7. Förfarande enligt patentkravet 6, kännetecknat därav, att den ytterligare kolkäl-lan är etanol, glukos eller glycerol. 30 104636 31
8. Förfarande enligt patentkravet 1, kännetecknat därav, att den nämnda transfor-merade jäststammen vidare transformeras med en andra DNA-sekvens som styr uttryck i den nämnda jäststammen av ett xylitoldehydrogenasenzym, vilket xylitol-dehydrogenasenzym möjliggör regenerering av en ko-faktor som krävs för xylos- 5 reduktasaktivitet.
9. Förfarande enligt patentkravet 8, kännetecknat därav, att den transformerade jäststammen tillhör arten Saccharomyces cerevisiae, Kluyveromyces sppSchi-zosaccharomyces pombe eller Pichia spp. 10
10. Förfarande enligt patentkravet 9, kännetecknat därav, att den transformerade jäststammen är Saccharomyces cerevisiae VTT-C-91181 som bar plasmiden pMW22 (NCYC nr 2352) som dessutom är transformerad med plasmiden pUA103, som anges i Fig. 1. 15
11. Enzymatiskt förfarande för framställning av xylitol, kännetecknat därav, att man a) odlar en rekombinant jäststam transformerad med en DNA-sekvens som kodar för xylosreduktasenzym under betingelser som möjliggör uttryck av det 20 nämnda xylosreduktaset, b) ätervinner det nämnda xylosreduktasenzymet, c) använder det nämnda xylosreduktaset i en enzymreaktor med ett ko-faktor- * regenererande system, och d) isolerar och renar bildad xylitol. 25 ^ J l • · <
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI924461A FI104636B (sv) | 1990-04-06 | 1992-10-02 | Hybridjäst, som innehåller DNA-sekvenser, som kodar för xylosreduktas- och xylitoldehydrogenasenzymer |
| FI992153A FI112250B (sv) | 1991-04-08 | 1999-10-06 | Förfarande för att framställa etanol, samt där tillämpande rekombinantjäststammar |
Applications Claiming Priority (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI901771A FI901771A (fi) | 1990-04-06 | 1990-04-06 | Anvaendning av xylos hos hybridjaest. |
| FI901771 | 1990-04-06 | ||
| US52777590A | 1990-05-24 | 1990-05-24 | |
| US52777590 | 1990-05-24 | ||
| PCT/FI1991/000103 WO1991015588A1 (en) | 1990-04-06 | 1991-04-08 | Recombinant yeasts containing the dna sequences coding for xylose reductase- and xylitol dehydrogenase enzymes |
| FI9100103 | 1991-04-08 | ||
| FI924461A FI104636B (sv) | 1990-04-06 | 1992-10-02 | Hybridjäst, som innehåller DNA-sekvenser, som kodar för xylosreduktas- och xylitoldehydrogenasenzymer |
| FI924461 | 1992-10-02 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI924461A0 FI924461A0 (fi) | 1992-10-02 |
| FI924461A FI924461A (fi) | 1992-10-02 |
| FI104636B true FI104636B (sv) | 2000-03-15 |
Family
ID=26158731
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI901771A FI901771A (fi) | 1990-04-06 | 1990-04-06 | Anvaendning av xylos hos hybridjaest. |
| FI924461A FI104636B (sv) | 1990-04-06 | 1992-10-02 | Hybridjäst, som innehåller DNA-sekvenser, som kodar för xylosreduktas- och xylitoldehydrogenasenzymer |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI901771A FI901771A (fi) | 1990-04-06 | 1990-04-06 | Anvaendning av xylos hos hybridjaest. |
Country Status (12)
| Country | Link |
|---|---|
| EP (1) | EP0527758B1 (sv) |
| JP (1) | JP3348215B2 (sv) |
| AT (1) | ATE161886T1 (sv) |
| AU (1) | AU647104B2 (sv) |
| CA (1) | CA2090122C (sv) |
| DE (1) | DE69128619T2 (sv) |
| DK (1) | DK0527758T3 (sv) |
| ES (1) | ES2113373T3 (sv) |
| FI (2) | FI901771A (sv) |
| GR (1) | GR3026489T3 (sv) |
| NO (1) | NO308544B1 (sv) |
| WO (1) | WO1991015588A1 (sv) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103695329A (zh) * | 2012-09-27 | 2014-04-02 | 远东新世纪股份有限公司 | 具有高木糖消耗率的分离酵母菌株和使用所述菌株产生乙醇的方法 |
| US9127323B2 (en) | 2012-09-27 | 2015-09-08 | Far Eastern New Century Corporation | Isolated yeast strain having high xylose consumption rate and process for production of ethanol using the strain |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5866382A (en) | 1990-04-06 | 1999-02-02 | Xyrofin Oy | Xylose utilization by recombinant yeasts |
| FI913197A0 (fi) | 1991-07-01 | 1991-07-01 | Xyrofin Oy | Nya jaeststammar med reducerad foermaoga att metabolisera xylitol, foerfarande foer bildande av dessa och deras anvaendning vid framstaellning av xylitol. |
| US6723540B1 (en) | 1992-11-05 | 2004-04-20 | Xyrofin Oy | Manufacture of xylitol using recombinant microbial hosts |
| PL178040B1 (pl) * | 1992-11-05 | 2000-02-29 | Xyrofin Oy | Sposób wytwarzania ksylitolu i zrekombinowany gospodarz drobnoustrojowy |
| US7226761B2 (en) | 1992-11-05 | 2007-06-05 | Danisco Sweeteners Oy | Manufacture of five-carbon sugars and sugar alcohols |
| EP0672161B1 (en) * | 1992-11-05 | 1999-09-22 | Xyrofin Oy | Recombinant method and host for manufacture of xylitol |
| US5789210A (en) * | 1993-11-08 | 1998-08-04 | Purdue Research Foundation | Recombinant yeasts for effective fermentation of glucose and xylose |
| IN191596B (sv) | 1996-05-06 | 2003-12-06 | Purdue Research Foundation | |
| FI980551A (sv) * | 1998-03-11 | 1999-09-12 | Valtion Teknillinen | Transformerade mikro-organismer med förbättrade egenskaper |
| EP1071786B1 (en) | 1998-04-20 | 2005-11-30 | Forskarpatent i Syd AB | Genetically engineered yeast and mutants thereof for the efficient fermentation of lignocellulose hydrolysates |
| DE60141811D1 (de) | 2000-01-21 | 2010-05-27 | Danisco | Herstellung von durch 5 kohlenstoffatome charakterisierte zucker und zuckeralkohole |
| US6894199B2 (en) | 2001-04-27 | 2005-05-17 | Danisco Sweeteners Oy | Process for the production of xylitol |
| FI20011889A (sv) | 2001-09-26 | 2003-03-27 | Xyrofin Oy | Förfarande för framställning av xylitol |
| EP1989303A2 (en) | 2006-02-27 | 2008-11-12 | Edenspace System Corporation | Energy crops for improved biofuel feedstocks |
| TWI438274B (zh) * | 2011-06-03 | 2014-05-21 | Inst Nuclear Energy Res Atomic Energy Council | 一種木糖代謝菌之製備方法及該木糖代謝菌 |
| CN104403956B (zh) * | 2013-09-05 | 2017-06-16 | 中国科学技术大学 | 木糖醇高温高产工程菌株的构建及应用 |
| BR112019013005A2 (pt) | 2016-12-21 | 2019-10-08 | Creatus Biosciences Inc | espécie de metschnikowia isolada, método para produzir xilitol, xilitol bioderivado e composição |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4009676A1 (de) * | 1990-03-26 | 1991-10-02 | Rhein Biotech Proz & Prod Gmbh | Dna-sequenz, umfassend ein fuer xylosereduktase und/oder xylitoldehydrogenase codierendes strukturgen |
-
1990
- 1990-04-06 FI FI901771A patent/FI901771A/fi not_active Application Discontinuation
-
1991
- 1991-04-08 ES ES91906996T patent/ES2113373T3/es not_active Expired - Lifetime
- 1991-04-08 DE DE69128619T patent/DE69128619T2/de not_active Expired - Fee Related
- 1991-04-08 DK DK91906996T patent/DK0527758T3/da active
- 1991-04-08 AT AT91906996T patent/ATE161886T1/de not_active IP Right Cessation
- 1991-04-08 CA CA002090122A patent/CA2090122C/en not_active Expired - Fee Related
- 1991-04-08 JP JP50690791A patent/JP3348215B2/ja not_active Expired - Fee Related
- 1991-04-08 WO PCT/FI1991/000103 patent/WO1991015588A1/en active IP Right Grant
- 1991-04-08 AU AU75657/91A patent/AU647104B2/en not_active Expired
- 1991-04-08 EP EP91906996A patent/EP0527758B1/en not_active Expired - Lifetime
-
1992
- 1992-10-02 FI FI924461A patent/FI104636B/sv not_active IP Right Cessation
- 1992-10-06 NO NO923880A patent/NO308544B1/no not_active IP Right Cessation
-
1998
- 1998-04-01 GR GR980400676T patent/GR3026489T3/el unknown
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103695329A (zh) * | 2012-09-27 | 2014-04-02 | 远东新世纪股份有限公司 | 具有高木糖消耗率的分离酵母菌株和使用所述菌株产生乙醇的方法 |
| US9127323B2 (en) | 2012-09-27 | 2015-09-08 | Far Eastern New Century Corporation | Isolated yeast strain having high xylose consumption rate and process for production of ethanol using the strain |
| CN103695329B (zh) * | 2012-09-27 | 2016-04-13 | 远东新世纪股份有限公司 | 具有高木糖消耗率的分离酵母菌株和使用所述菌株产生乙醇的方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| AU7565791A (en) | 1991-10-30 |
| CA2090122C (en) | 2002-06-18 |
| ATE161886T1 (de) | 1998-01-15 |
| FI924461A0 (fi) | 1992-10-02 |
| WO1991015588A1 (en) | 1991-10-17 |
| NO923880L (no) | 1992-10-06 |
| NO923880D0 (no) | 1992-10-06 |
| DE69128619D1 (de) | 1998-02-12 |
| DE69128619T2 (de) | 1998-08-20 |
| FI924461A (fi) | 1992-10-02 |
| GR3026489T3 (en) | 1998-07-31 |
| AU647104B2 (en) | 1994-03-17 |
| DK0527758T3 (da) | 1998-09-07 |
| FI901771A0 (fi) | 1990-04-06 |
| ES2113373T3 (es) | 1998-05-01 |
| EP0527758A1 (en) | 1993-02-24 |
| NO308544B1 (no) | 2000-09-25 |
| JP3348215B2 (ja) | 2002-11-20 |
| JPH05507843A (ja) | 1993-11-11 |
| EP0527758B1 (en) | 1998-01-07 |
| CA2090122A1 (en) | 1991-10-07 |
| FI901771A (fi) | 1991-10-07 |
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| MA | Patent expired |