ES2113373T3 - Levaduras recombinantes que contienen secuencias de adn que codifican las enzimas xilosa-reductasa y xilitol-deshidrogenasa. - Google Patents
Levaduras recombinantes que contienen secuencias de adn que codifican las enzimas xilosa-reductasa y xilitol-deshidrogenasa.Info
- Publication number
- ES2113373T3 ES2113373T3 ES91906996T ES91906996T ES2113373T3 ES 2113373 T3 ES2113373 T3 ES 2113373T3 ES 91906996 T ES91906996 T ES 91906996T ES 91906996 T ES91906996 T ES 91906996T ES 2113373 T3 ES2113373 T3 ES 2113373T3
- Authority
- ES
- Spain
- Prior art keywords
- xylitol
- recombinant
- reductase
- xylosa
- yeas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Detergent Compositions (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
ESTA INVENCION ESTA RELACIONADA CON LA TECNOLOGIA DEL ADN RECOMBINANTE. ESPECIFICAMENTE, ESTA INVENCION ESTA RELACIONADA CON CEPAS DE LEVADURA RECOMBINANTES NUEVAS TRANSFORMADAS CON GENES DE ENZIMAS DE REDUCTASA DE XILOSA Y/O DEHIDROGENASA DE XILITOL. UNA CEPA DE LEVADURA TRANSFORMADA CON EL GEN DE REDUCTASA DE XILOSA ES CAPAZ DE REDUCIR LA XILOSA O EL XILITOL Y, CONSECUENTEMENTE, DE PRODUCIR XILITOL IN VIVO. SI ESTOS DOS GENES SE TRANSFORMAN EN UNA CEPA DE LEVADURA, LA CEPA RESULTANTE PUEDE PRODUCIR ETANOL EN UN MEDIO QUE CONTIENE XILOSA DURANTE LA FERMENTACION. ADEMAS, DICHAS CEPAS DE LEVADURA NUEVAS PUEDEN EXPRESAR AMBOS ENZIMAS CITADOS. SE PUEDE UTILIZAR LA REDUCTASA DE XILOSA PRODUCIDA POR ESTAS CEPAS EN UN PROCESO ENZIMATICO PARA LA PRODUCCION DE XILITOL IN VITRO.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FI901771A FI901771A (fi) | 1990-04-06 | 1990-04-06 | Anvaendning av xylos hos hybridjaest. |
US52777590A | 1990-05-24 | 1990-05-24 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2113373T3 true ES2113373T3 (es) | 1998-05-01 |
Family
ID=26158731
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES91906996T Expired - Lifetime ES2113373T3 (es) | 1990-04-06 | 1991-04-08 | Levaduras recombinantes que contienen secuencias de adn que codifican las enzimas xilosa-reductasa y xilitol-deshidrogenasa. |
Country Status (12)
Country | Link |
---|---|
EP (1) | EP0527758B1 (es) |
JP (1) | JP3348215B2 (es) |
AT (1) | ATE161886T1 (es) |
AU (1) | AU647104B2 (es) |
CA (1) | CA2090122C (es) |
DE (1) | DE69128619T2 (es) |
DK (1) | DK0527758T3 (es) |
ES (1) | ES2113373T3 (es) |
FI (2) | FI901771A (es) |
GR (1) | GR3026489T3 (es) |
NO (1) | NO308544B1 (es) |
WO (1) | WO1991015588A1 (es) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5866382A (en) | 1990-04-06 | 1999-02-02 | Xyrofin Oy | Xylose utilization by recombinant yeasts |
FI913197A0 (fi) | 1991-07-01 | 1991-07-01 | Xyrofin Oy | Nya jaeststammar med reducerad foermaoga att metabolisera xylitol, foerfarande foer bildande av dessa och deras anvaendning vid framstaellning av xylitol. |
US7226761B2 (en) | 1992-11-05 | 2007-06-05 | Danisco Sweeteners Oy | Manufacture of five-carbon sugars and sugar alcohols |
PL178040B1 (pl) * | 1992-11-05 | 2000-02-29 | Xyrofin Oy | Sposób wytwarzania ksylitolu i zrekombinowany gospodarz drobnoustrojowy |
US6723540B1 (en) | 1992-11-05 | 2004-04-20 | Xyrofin Oy | Manufacture of xylitol using recombinant microbial hosts |
EP0672161B1 (en) * | 1992-11-05 | 1999-09-22 | Xyrofin Oy | Recombinant method and host for manufacture of xylitol |
US5789210A (en) * | 1993-11-08 | 1998-08-04 | Purdue Research Foundation | Recombinant yeasts for effective fermentation of glucose and xylose |
IN191596B (es) * | 1996-05-06 | 2003-12-06 | Purdue Research Foundation | |
FI980551A (fi) * | 1998-03-11 | 1999-09-12 | Valtion Teknillinen | Transformoidut mikro-organismit, joilla on parannettuja ominaisuuksia |
JP2002512037A (ja) | 1998-04-20 | 2002-04-23 | フォルスカルパテント イー エスイーデー アクティエボラーグ | リグノセルロース加水分解産物の効率の良い発酵のための遺伝子操作された酵母及びその突然変異体 |
JP2003520583A (ja) | 2000-01-21 | 2003-07-08 | ダニスコ スイートナーズ オイ | 五炭糖及び糖アルコールの製造 |
US6894199B2 (en) | 2001-04-27 | 2005-05-17 | Danisco Sweeteners Oy | Process for the production of xylitol |
FI20011889A (fi) | 2001-09-26 | 2003-03-27 | Xyrofin Oy | Menetelmä ksylitolin valmistamiseksi |
WO2007100897A2 (en) | 2006-02-27 | 2007-09-07 | Edenspace System Corporation | Energy crops for improved biofuel feedstocks |
TWI438274B (zh) * | 2011-06-03 | 2014-05-21 | Inst Nuclear Energy Res Atomic Energy Council | 一種木糖代謝菌之製備方法及該木糖代謝菌 |
TWI450963B (zh) * | 2012-09-27 | 2014-09-01 | Far Eastern New Century Corp | 具高木醣消耗率之分離酵母菌株及使用該菌株製造酒精之方法 |
US9127323B2 (en) | 2012-09-27 | 2015-09-08 | Far Eastern New Century Corporation | Isolated yeast strain having high xylose consumption rate and process for production of ethanol using the strain |
CN104403956B (zh) * | 2013-09-05 | 2017-06-16 | 中国科学技术大学 | 木糖醇高温高产工程菌株的构建及应用 |
EP3559207A4 (en) | 2016-12-21 | 2020-08-12 | Creatus Biosciences Inc. | XYLITOL-PRODUCING METSCHNIKOWIA SPECIES |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4009676A1 (de) * | 1990-03-26 | 1991-10-02 | Rhein Biotech Proz & Prod Gmbh | Dna-sequenz, umfassend ein fuer xylosereduktase und/oder xylitoldehydrogenase codierendes strukturgen |
-
1990
- 1990-04-06 FI FI901771A patent/FI901771A/fi not_active Application Discontinuation
-
1991
- 1991-04-08 ES ES91906996T patent/ES2113373T3/es not_active Expired - Lifetime
- 1991-04-08 AT AT91906996T patent/ATE161886T1/de not_active IP Right Cessation
- 1991-04-08 CA CA002090122A patent/CA2090122C/en not_active Expired - Fee Related
- 1991-04-08 DK DK91906996T patent/DK0527758T3/da active
- 1991-04-08 WO PCT/FI1991/000103 patent/WO1991015588A1/en active IP Right Grant
- 1991-04-08 JP JP50690791A patent/JP3348215B2/ja not_active Expired - Fee Related
- 1991-04-08 AU AU75657/91A patent/AU647104B2/en not_active Expired
- 1991-04-08 EP EP91906996A patent/EP0527758B1/en not_active Expired - Lifetime
- 1991-04-08 DE DE69128619T patent/DE69128619T2/de not_active Expired - Fee Related
-
1992
- 1992-10-02 FI FI924461A patent/FI104636B/fi not_active IP Right Cessation
- 1992-10-06 NO NO923880A patent/NO308544B1/no not_active IP Right Cessation
-
1998
- 1998-04-01 GR GR980400676T patent/GR3026489T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
CA2090122A1 (en) | 1991-10-07 |
DE69128619D1 (de) | 1998-02-12 |
FI924461A0 (fi) | 1992-10-02 |
FI901771A (fi) | 1991-10-07 |
NO308544B1 (no) | 2000-09-25 |
ATE161886T1 (de) | 1998-01-15 |
NO923880L (no) | 1992-10-06 |
GR3026489T3 (en) | 1998-07-31 |
WO1991015588A1 (en) | 1991-10-17 |
AU7565791A (en) | 1991-10-30 |
JP3348215B2 (ja) | 2002-11-20 |
EP0527758B1 (en) | 1998-01-07 |
DE69128619T2 (de) | 1998-08-20 |
AU647104B2 (en) | 1994-03-17 |
JPH05507843A (ja) | 1993-11-11 |
FI924461A (fi) | 1992-10-02 |
FI901771A0 (fi) | 1990-04-06 |
DK0527758T3 (da) | 1998-09-07 |
EP0527758A1 (en) | 1993-02-24 |
FI104636B (fi) | 2000-03-15 |
CA2090122C (en) | 2002-06-18 |
NO923880D0 (no) | 1992-10-06 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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