ES8609727A1 - Sistema de agentes reactivos - Google Patents

Sistema de agentes reactivos

Info

Publication number
ES8609727A1
ES8609727A1 ES543657A ES543657A ES8609727A1 ES 8609727 A1 ES8609727 A1 ES 8609727A1 ES 543657 A ES543657 A ES 543657A ES 543657 A ES543657 A ES 543657A ES 8609727 A1 ES8609727 A1 ES 8609727A1
Authority
ES
Spain
Prior art keywords
leukocyte
leukocytes
reagent
volume
size
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES543657A
Other languages
English (en)
Other versions
ES543657A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coulter Electronics Inc
Original Assignee
Coulter Electronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Coulter Electronics Inc filed Critical Coulter Electronics Inc
Publication of ES543657A0 publication Critical patent/ES543657A0/es
Publication of ES8609727A1 publication Critical patent/ES8609727A1/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Ecology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PROCEDIMIENTO PARA PREPARAR UN SISTEMA DE AGENTES REACTIVOS A BASE DE SAPONINA. COMPRENDE: A) DILUIR POLVO DE SAPONINA DE GRADO TECNICO, UNA SAL DE LISADO DE CELULAS Y (A) EN 1 LITRO DE AGUA DESIONIZADA PARA FORMAR UNA DISOLUCION; B) FILTRAR LA DISOLUCION DE A), PARA FORMAR UN DILUENTE LITICO; C) DISOLVER A (A) Y UNA SUSTANCIA PARA ASEGURAR UN PH ADECUADO EN UN LITRO DE AGUA DESIONIZADA; D) FILTRAR LA SOLUCION PRE-DILUENTE DE C); E) DILUIR POLVO DE SAPONINA DE GRADO TECNICO Y (A) EN AGUA DESIONIZADA; F) INCORPORAR UN PRESERVATIVO A LA DILUCION DE A) Y FILTRAR, PARA OBTENER UN AGENTE REACTIVO LITICO; G) DILUIR UN AGENTE DE ENTRECRUZAMIENTO Y (A) EN AGUA DESIONIZADA; H) FILTRAR LA SOLUCION DE G), PARA OBTENER UN AGENTE REACTIVO DE FIJACION E I) MANTENER A LOS AGENTES DE FIJACION, AL PREDILUYENTE Y AL AGENTE REACTIVO LITICO A CONCENTRACION, PH Y OSMOLARIDAD ADECUADOS. EL DILUYENTE LITICO ES UNA MEZCLA DE 0,15 A 0,16% EN PESO/VOLUMEN DE SAPONINA, 0,2 A 0,6 PESO/VOLUMEN DE CLORURO DE SODIO Y 0,01 O 0,1% PESO/VOLUMEN DE ACIDO SORBICO; EL AGENTE REACTIVO DE LISADO CONTIENE SALES DE METALES ALCALINOS, 0,2 A 0,6% PESO/VOLUMEN Y 0,3 A 4,0 DE SAPONINA; EL AGENTE DE ENTRECRUZAMIENTO ES GLUTARALDEHIDO. SIENDO A UNA SUSTANCIA PARA CONTROLAR LOS TAMAÑOS DE LAS CELULAS DE LAS PRINCIPALES CATEGORIAS DE LEUCOCITOS (MONOCITOS, LINFOCITOS Y EOSINOFILOS).
ES543657A 1984-05-31 1985-05-30 Sistema de agentes reactivos Expired ES8609727A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/615,966 US4751179A (en) 1984-05-31 1984-05-31 Method and reagents for differential determination of four populations of leukocytes in blood

Publications (2)

Publication Number Publication Date
ES543657A0 ES543657A0 (es) 1986-08-16
ES8609727A1 true ES8609727A1 (es) 1986-08-16

Family

ID=24467496

Family Applications (1)

Application Number Title Priority Date Filing Date
ES543657A Expired ES8609727A1 (es) 1984-05-31 1985-05-30 Sistema de agentes reactivos

Country Status (9)

Country Link
US (1) US4751179A (es)
EP (1) EP0185048B1 (es)
JP (1) JPS61502277A (es)
KR (1) KR860700153A (es)
AU (1) AU4401985A (es)
CA (1) CA1248856A (es)
DE (1) DE3587036T2 (es)
ES (1) ES8609727A1 (es)
WO (1) WO1985005684A1 (es)

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* Cited by examiner, † Cited by third party
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Also Published As

Publication number Publication date
AU4401985A (en) 1985-12-31
JPS61502277A (ja) 1986-10-09
EP0185048A4 (es) 1988-09-28
DE3587036D1 (de) 1993-03-11
CA1248856A (en) 1989-01-17
EP0185048A1 (es) 1986-06-25
DE3587036T2 (de) 1993-09-02
ES543657A0 (es) 1986-08-16
US4751179A (en) 1988-06-14
EP0185048B1 (en) 1993-01-27
KR860700153A (ko) 1986-03-31
WO1985005684A1 (en) 1985-12-19

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