ES8602133A1 - Un procedimiento para preparar un vector de transferencias de adn - Google Patents

Un procedimiento para preparar un vector de transferencias de adn

Info

Publication number
ES8602133A1
ES8602133A1 ES527964A ES527964A ES8602133A1 ES 8602133 A1 ES8602133 A1 ES 8602133A1 ES 527964 A ES527964 A ES 527964A ES 527964 A ES527964 A ES 527964A ES 8602133 A1 ES8602133 A1 ES 8602133A1
Authority
ES
Spain
Prior art keywords
characterization
molecular cloning
gene sequence
sequence coding
human relaxin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES527964A
Other languages
English (en)
Other versions
ES527964A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Florey Institute of Neuroscience and Mental Health
Original Assignee
Howard Florey Institute of Experimental Physiology and Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=3769889&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES8602133(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Howard Florey Institute of Experimental Physiology and Medicine filed Critical Howard Florey Institute of Experimental Physiology and Medicine
Publication of ES8602133A1 publication Critical patent/ES8602133A1/es
Publication of ES527964A0 publication Critical patent/ES527964A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/64—Relaxins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/02—Drugs for disorders of the urinary system of urine or of the urinary tract, e.g. urine acidifiers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Veterinary Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Reproductive Health (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

OBTENCION DE PREPRORRELAXINA -H2 HUMANA O UNA SUBUNIDAD DE LA MISMA MEDIANTE TECNICAS DE INGENIERIA GENETICA. SE AISLA ARN PROCEDENTE DE TEJIDO OVARICO HUMANO, SE GENERA SU ADN DE DOBLE CADENA CORRESPONDIENTE, QUE SE CLONA A UN VECTOR PLASMIDO PBR322 O LGT10. SE PREPARAN UNAS SONDAS DE HIBRIDACION RADIOMARCADAS MEDIANTE SINTESIS INICIADA SOBRE DIVERSOS FRAGMENTOS DE ADN MANDO INICIADORES AL AZAR DESNATURALIZADOS DEL ADN DE TIMO DE TERNERA. SE SELECCIONAN LAS SECUENCIAS DE GENES PARA PREPRORRELAXINA, PRORRELAXINA, RELAXINA Y LOS PEPTIDOS DE SEÑAL A, B Y C CORRESPONDIENTES AL GEH H2 HUMANO, SE PURIFICAN Y SE ESTUDIA SU ACTIVIDAD. SE LIGA UNA SECUENCIA DE DESOXINUCLEOTIDOS QUE CODIFICA PREPRORRELAXINA HUMANA CON UNA MOLECULA DE ADN PREPARADA ESCINDIENDO UN VECTOR DE TRANSFERENCIA CON UNA ENZIMA DE RESTRICCION. SE TRANSFORMA UN CULTIVO CELULAR DE E&COLI, SE INCUBA Y SE OBTIENE LA PROTEINA DE FUSION.
ES527964A 1982-12-13 1983-12-12 Un procedimiento para preparar un vector de transferencias de adn Granted ES527964A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AUPF724782 1982-12-13

Publications (2)

Publication Number Publication Date
ES8602133A1 true ES8602133A1 (es) 1985-11-16
ES527964A0 ES527964A0 (es) 1985-11-16

Family

ID=3769889

Family Applications (6)

Application Number Title Priority Date Filing Date
ES527964A Granted ES527964A0 (es) 1982-12-13 1983-12-12 Un procedimiento para preparar un vector de transferencias de adn
ES544293A Expired ES8802249A1 (es) 1982-12-13 1985-06-17 Un procedimiento para el aislamiento de un gen de relaxina de un ser humano u otro primate o de una especie relacionada
ES552094A Expired ES8708015A1 (es) 1982-12-13 1986-02-17 Un procedimiento para preparar relaxina -h2 humana o uno de sus analogos
ES552095A Expired ES8801373A1 (es) 1982-12-13 1986-02-17 Un procedimiento para aislar un gen de preprorrelaxina -h2 humana o un equivalente o subunidad del mismo
ES552093A Expired ES8708016A1 (es) 1982-12-13 1986-02-17 Un procedimiento para sintetizar una secuencia de aminoacidos que contienen la totalidad o parte de la secuencia de aminoacidos de la preprorrelaxina -h2 humana.
ES557531A Expired ES8801675A1 (es) 1982-12-13 1987-05-11 Un procedimiento para preparar relaxina-h2 humana

Family Applications After (5)

Application Number Title Priority Date Filing Date
ES544293A Expired ES8802249A1 (es) 1982-12-13 1985-06-17 Un procedimiento para el aislamiento de un gen de relaxina de un ser humano u otro primate o de una especie relacionada
ES552094A Expired ES8708015A1 (es) 1982-12-13 1986-02-17 Un procedimiento para preparar relaxina -h2 humana o uno de sus analogos
ES552095A Expired ES8801373A1 (es) 1982-12-13 1986-02-17 Un procedimiento para aislar un gen de preprorrelaxina -h2 humana o un equivalente o subunidad del mismo
ES552093A Expired ES8708016A1 (es) 1982-12-13 1986-02-17 Un procedimiento para sintetizar una secuencia de aminoacidos que contienen la totalidad o parte de la secuencia de aminoacidos de la preprorrelaxina -h2 humana.
ES557531A Expired ES8801675A1 (es) 1982-12-13 1987-05-11 Un procedimiento para preparar relaxina-h2 humana

Country Status (14)

Country Link
US (1) US4758516A (es)
EP (2) EP0112149B1 (es)
JP (3) JP2545055B2 (es)
KR (1) KR940002536B1 (es)
AT (2) ATE90360T1 (es)
CA (1) CA1220735A (es)
DE (1) DE3382249D1 (es)
DK (2) DK171684B1 (es)
ES (6) ES527964A0 (es)
HU (1) HU197767B (es)
IE (1) IE63902B1 (es)
IL (1) IL70414A (es)
NZ (1) NZ206534A (es)
ZA (1) ZA839231B (es)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0287820B1 (en) * 1982-08-12 1992-07-08 Howard Florey Institute Of Experimental Physiology And Medicine Human relaxin analogues
US5179195A (en) * 1982-12-13 1993-01-12 Howard Florey Institute Of Experimental Physiology And Medicine Human relaxin polypeptides
US4835251A (en) * 1986-06-23 1989-05-30 Genetech, Inc. Method of chain combination
AU612594B2 (en) * 1986-09-12 1991-07-18 Genentech Inc. Human prorelaxin
WO1989007945A1 (en) * 1988-02-26 1989-09-08 Genentech, Inc. Human relaxin formulation
JP2857684B2 (ja) * 1989-05-04 1999-02-17 ジェネンテク，インコーポレイテッド ヒトリラキシンの単離のための方法および組成物
US5089419A (en) * 1989-08-07 1992-02-18 International Canine Genetics Detection of pregnancy by identification of the c peptide of relaxin in the urine of animals
US5166191A (en) * 1991-08-19 1992-11-24 Genentech, Inc. Use of relaxin in cardiovascular therapy
US5478807A (en) * 1991-08-19 1995-12-26 Genentech, Inc. Use of relaxin in the treatment of bradycardia
EP0611451A1 (en) * 1991-10-28 1994-08-24 The Board Of Regents, The University Of Texas System A method for detecting a differentiation marker in normal and carcinoma cells and in biological samples and body fluid
US6348327B1 (en) 1991-12-06 2002-02-19 Genentech, Inc. Non-endocrine animal host cells capable of expressing variant proinsulin and processing the same to form active, mature insulin and methods of culturing such cells
EP0707650B1 (en) * 1993-06-21 2003-05-21 Genentech, Inc. Process for producing human relaxin
US20050032683A1 (en) * 2000-10-04 2005-02-10 Amento Edward P. Methods of modulating apoptosis by administration of relaxin agonists or antagonists
US20040006205A1 (en) * 2001-04-03 2004-01-08 Li Li Therapeutic polypeptides, nucleic acids encoding same, and methods of use
CA2456753A1 (en) * 2001-08-17 2003-02-27 The Board Of Trustees Of The Leland Stanford Junior University Mammalian relaxin receptors
AU2003903124A0 (en) * 2003-06-20 2003-07-10 Mark Del Borgo Analogues of heteromeric proteins
CA2563433C (en) * 2004-04-30 2010-07-13 Bas Medical, Inc. Methods and compositions for control of fetal growth via modulation of relaxin
US20060052304A1 (en) * 2004-09-02 2006-03-09 Bas Medical, Inc. Method for remodeling bone and related sutures
CN102026650A (zh) * 2008-05-16 2011-04-20 科尔泰拉公司 预防早产的方法
GR1006941B (el) 2009-06-01 2010-08-27 Χημικα Και Βιοφαρμακευτικα Εργαστηρια Πατρων Αε (Cbl-Patras), Πεπτιδικη συνθεση (peptide synthesis)
US8389475B2 (en) * 2009-08-10 2013-03-05 The Board Of Trustees Of The Leland Stanford Junior University Relaxin analogs
ES2972902T3 (es) 2010-08-17 2024-06-17 Ambrx Inc Polipéptidos de relaxina modificados y sus usos
KR20140054009A (ko) 2011-07-01 2014-05-08 바이엘 인텔렉쳐 프로퍼티 게엠베하 릴랙신 융합 폴리펩타이드 및 그의 용도
EP2630964A1 (en) 2012-02-22 2013-08-28 Immundiagnostik AG Method and medicament for treating patients in risk of prediabetes and type-2 diabetes
US20160296632A1 (en) 2013-11-13 2016-10-13 Aequus Biopharma, Inc. Engineered glycoproteins and uses thereof
EP2946788A1 (en) 2014-05-23 2015-11-25 Immundiagnostik AG Method and composition for treating heart failure with preserved ejection fraction
IL263079B2 (en) 2016-05-18 2024-05-01 Modernatx Inc Relaxin-encoding polynucleotides
AU2018219283B2 (en) 2017-02-08 2022-05-19 Bristol-Myers Squibb Company Modified relaxin polypeptides comprising a pharmacokinetic enhancer and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4468464A (en) * 1974-11-04 1984-08-28 The Board Of Trustees Of The Leland Stanford Junior University Biologically functional molecular chimeras
GR70279B (es) * 1979-09-12 1982-09-03 Univ California
US4267101A (en) * 1980-02-01 1981-05-12 Serono Laboratories Inc. Process for obtaining human relaxin from fetal membranes
EP0068375A3 (en) * 1981-06-22 1983-04-13 G.D. Searle & Co. Recombinant dna techniques for the production of relaxin
IE55479B1 (en) * 1982-02-12 1990-09-26 Florey Howard Inst Molecular cloning and characterization of the gene sequence coding for porcine relaxin
EP0287820B1 (en) * 1982-08-12 1992-07-08 Howard Florey Institute Of Experimental Physiology And Medicine Human relaxin analogues

Also Published As

Publication number Publication date
IL70414A (en) 1991-06-10
ES8708016A1 (es) 1987-09-01
DE3382249D1 (de) 1991-05-16
ES8708015A1 (es) 1987-09-01
ZA839231B (en) 1984-08-29
JP2677332B2 (ja) 1997-11-17
JPH07173198A (ja) 1995-07-11
DK573783D0 (da) 1983-12-13
ES8801675A1 (es) 1988-02-16
IE832908L (en) 1984-06-13
JPS59156284A (ja) 1984-09-05
US4758516A (en) 1988-07-19
JPH06100585A (ja) 1994-04-12
EP0112149B1 (en) 1991-04-10
IL70414A0 (en) 1984-03-30
IE63902B1 (en) 1995-06-14
ES8801373A1 (es) 1987-12-16
DK172012B1 (da) 1997-09-15
EP0303033B1 (en) 1993-06-09
ES8802249A1 (es) 1988-04-16
ES552094A0 (es) 1987-09-01
ES552093A0 (es) 1987-09-01
JP2891882B2 (ja) 1999-05-17
ATE90360T1 (de) 1993-06-15
DK143492A (da) 1992-11-30
KR840007024A (ko) 1984-12-04
HU197767B (en) 1989-05-29
ES544293A0 (es) 1988-04-16
DK143492D0 (da) 1992-11-30
NZ206534A (en) 1988-05-30
ES552095A0 (es) 1987-12-16
CA1220735A (en) 1987-04-21
DK573783A (da) 1984-06-14
JP2545055B2 (ja) 1996-10-16
EP0112149A2 (en) 1984-06-27
EP0303033A2 (en) 1989-02-15
DK171684B1 (da) 1997-03-10
EP0112149A3 (en) 1985-05-22
EP0303033A3 (en) 1989-10-25
KR940002536B1 (ko) 1994-03-25
ES527964A0 (es) 1985-11-16
ES557531A0 (es) 1988-02-16
ATE62507T1 (de) 1991-04-15

Similar Documents

Publication Publication Date Title
ES8708016A1 (es) Un procedimiento para sintetizar una secuencia de aminoacidos que contienen la totalidad o parte de la secuencia de aminoacidos de la preprorrelaxina -h2 humana.
ES8801676A1 (es) Un procedimiento para preparar relaxina humana o sus analogos
NO885659D0 (no) Mycobakterielle rekombinanter og peptider.
ES2009238A6 (es) Metodo para producir proteinas de mycobacterium tuberculosis y peptidos antigenicos de las mismas.
DK0932678T3 (da) En familie af gener kodende for apoptoserelaterede peptider, peptider kodet deraf og fremgangsmåde til anvendelse deraf
DK0882130T5 (da) Humant cancerantigen i tyrosinase-beslægtet protein 1 og gen, der koder dette
FI941048A (fi) Geloniinipolypeptidin koodaavia DNA-sekvenssejä
DE3686579D1 (en) Prokaryotisches expressionssystem.
ES8503373A1 (es) Un procedimiento de preparacion de un vector de transferencia de adn.
DK595187A (da) Minactivin og fremgangsmaade til fremstilling deraf
SE8100238L (sv) Syntetisk gen, plasmidvektor innehallande denna gen, forfaranden for genens och vektorns framstellning och anvendning samt salunda erhallna celler och peptider
ES2008391A6 (es) Un metodo para producir isopenicilina n sintetasa.
WO2000053789A3 (de) Retrovirale expressionsvektoren auf der basis von herv-ltr-sequenzen
HUP9903075A2 (hu) Sejtciklus ellenőrzőpont gének

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20000601