WO2000053789A3 - Retrovirale expressionsvektoren auf der basis von herv-ltr-sequenzen - Google Patents

Retrovirale expressionsvektoren auf der basis von herv-ltr-sequenzen Download PDF

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Publication number
WO2000053789A3
WO2000053789A3 PCT/EP2000/002064 EP0002064W WO0053789A3 WO 2000053789 A3 WO2000053789 A3 WO 2000053789A3 EP 0002064 W EP0002064 W EP 0002064W WO 0053789 A3 WO0053789 A3 WO 0053789A3
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WO
WIPO (PCT)
Prior art keywords
cell
expression vectors
herv
retroviral
retroviral expression
Prior art date
Application number
PCT/EP2000/002064
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English (en)
French (fr)
Other versions
WO2000053789A2 (de
Inventor
Christine Leib-Moesch
Ulrike Schoen
Corinna Baust
Original Assignee
Gsf Forschungszentrum Umwelt
Leib Moesch Christine
Ulrike Schoen
Corinna Baust
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gsf Forschungszentrum Umwelt, Leib Moesch Christine, Ulrike Schoen, Corinna Baust filed Critical Gsf Forschungszentrum Umwelt
Priority to EP00918779A priority Critical patent/EP1144667A2/de
Priority to JP2000603410A priority patent/JP2002537846A/ja
Publication of WO2000053789A2 publication Critical patent/WO2000053789A2/de
Publication of WO2000053789A3 publication Critical patent/WO2000053789A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung betrifft retrovirale Expressionsvektoren mit zellspezifisch regulierbaren Promotoren. Die Vektoren sind beispielsweise zur zellspezifischen Expression therapeutisch wertvoller Gene im Rahmen einer Gentherapie einsetzbar. Die Erfindung beschreibt retrovirale Expressionsvektoren, enthaltend zumindest die nachfolgenden Elemente in funktioneller Anordnung: a) DNA-Sequenzen zur Verpackung der Vektor-RNA und zur zellspezifischen Expression von Proteinen oder Peptiden, die von heterologen DNA-Nukleotidsequenzen kodiert werden; b) eine oder mehrere für ein Protein oder Peptid kodierende DNA-Nukleotidsequenzen, dadurch gekennzeichnet, daß die DNA-Sequenzen zur zellspezifischen Expression eine zellspezifisch regulierbare Promotorregion aus einer humanen endogenen retroviralen DNA-Nukleotidsequenz (HERV) enthalten.
PCT/EP2000/002064 1999-03-10 2000-03-09 Retrovirale expressionsvektoren auf der basis von herv-ltr-sequenzen WO2000053789A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00918779A EP1144667A2 (de) 1999-03-10 2000-03-09 Retrovirale expressionsvektoren auf der basis von herv-ltr-sequenzen
JP2000603410A JP2002537846A (ja) 1999-03-10 2000-03-09 Ｈｅｒｖｌｔｒ配列に基づく、レトロウイルス発現ベクター

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19910650.9 1999-03-10
DE19910650A DE19910650A1 (de) 1999-03-10 1999-03-10 Auf HERV-LTR-Sequenzen basierende retrovirale Expressionsvektoren

Publications (2)

Publication Number Publication Date
WO2000053789A2 WO2000053789A2 (de) 2000-09-14
WO2000053789A3 true WO2000053789A3 (de) 2001-04-05

Family

ID=7900460

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/002064 WO2000053789A2 (de) 1999-03-10 2000-03-09 Retrovirale expressionsvektoren auf der basis von herv-ltr-sequenzen

Country Status (4)

Country Link
EP (1) EP1144667A2 (de)
JP (1) JP2002537846A (de)
DE (1) DE19910650A1 (de)
WO (1) WO2000053789A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT411262B (de) 2001-09-27 2003-11-25 Wolff Klaus Dr Humanes endogenes retrovirus

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996028563A1 (en) * 1995-03-09 1996-09-19 Bavarian Nordic Vectors carrying therapeutic genes encoding antimicrobial peptides for gene therapy
WO2000023606A1 (en) * 1998-10-22 2000-04-27 Medical College Of Georgia Institute, Inc. Long terminal repeat, enhancer, and insulator sequences for use in recombinant vectors

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX9701544A (es) * 1994-09-02 1998-03-31 Gsf Forschungszentrum Umwelt Vectores de expresion retrovirales dirigidos, no autoinactivantes.
ATE212061T1 (de) * 1995-03-09 2002-02-15 Austrian Nordic Biotherapeutic Rekombinante dna-vektoren für die gentherapie

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996028563A1 (en) * 1995-03-09 1996-09-19 Bavarian Nordic Vectors carrying therapeutic genes encoding antimicrobial peptides for gene therapy
WO2000023606A1 (en) * 1998-10-22 2000-04-27 Medical College Of Georgia Institute, Inc. Long terminal repeat, enhancer, and insulator sequences for use in recombinant vectors

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ANDERSSEN S. ET AL.: "Comparative analyses of LTRs of the ERV-H family of primate-specific retrovirus-like elements isolated from marmoset, African green monkey, and man.", VIROLOGY, vol. 234, no. 1, 1997, pages 14 - 30, XP002132290, ISSN: 0042-6822 *
SCHOEN U. ET AL: "Tissue specific expression and promoter activity of different HERV - LTRs.", JOURNAL OF MOLECULAR MEDICINE, vol. 75, no. 7, 1997, pages B228, XP000946480 *
SJOTTEM E ET AL: "The promoter activity of long terminal repeats of the HERV -H family of human retrovirus-like elements is critically dependent on Sp1 family proteins interacting with a GC/GT box located immediately 3' to the TATA box.", JOURNAL OF VIROLOGY, vol. 70, no. 1, January 1996 (1996-01-01), pages 188 - 198, XP002148194 *

Also Published As

Publication number Publication date
JP2002537846A (ja) 2002-11-12
WO2000053789A2 (de) 2000-09-14
DE19910650A1 (de) 2000-09-21
EP1144667A2 (de) 2001-10-17

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