ES8401134A1 - "metodo de producir un cultivo transformante de celulas de vertebrado". - Google Patents
"metodo de producir un cultivo transformante de celulas de vertebrado".Info
- Publication number
- ES8401134A1 ES8401134A1 ES515260A ES515260A ES8401134A1 ES 8401134 A1 ES8401134 A1 ES 8401134A1 ES 515260 A ES515260 A ES 515260A ES 515260 A ES515260 A ES 515260A ES 8401134 A1 ES8401134 A1 ES 8401134A1
- Authority
- ES
- Spain
- Prior art keywords
- hbsag
- cell culture
- gene
- vertebrate cell
- polypeptides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 3
- 241000251539 Vertebrata <Metazoa> Species 0.000 title abstract 2
- 238000004113 cell culture Methods 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 239000013598 vector Substances 0.000 abstract 2
- 241000282693 Cercopithecidae Species 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 230000009089 cytolysis Effects 0.000 abstract 1
- 210000002950 fibroblast Anatomy 0.000 abstract 1
- 229960002520 hepatitis vaccine Drugs 0.000 abstract 1
- 210000003734 kidney Anatomy 0.000 abstract 1
- 238000009877 rendering Methods 0.000 abstract 1
- 230000010076 replication Effects 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2730/00—Reverse transcribing DNA viruses
- C12N2730/00011—Details
- C12N2730/10011—Hepadnaviridae
- C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
- C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
PROCEDIMIENTO PARA LA PRODUCCION DE CULTIVOS TRANSFORMANTES DE CELULAS DE VERTEBRADO, CAPACES DE PRODUCIR POLIPEPTIDOS, POR EXPRESION DE VECTORES DE EXPRESION REPLICABLES, QUE CODIFICAN LOS POLIPEPTIDOS.CONSISTE EN PREPARAR UN FRAGMENTO DE VECTOR DE TRANSFERENCIA DE ADN CAPAZ DE REPLICACION DE UN CULTIVO DE CELULAS HOSPEDANTES. PREPARAR UN FRAGMENTO DE ADN QUE CONTIENE UN PROMOTOR COMPATIBLE CON EL CULTIVO, Y UN FRAGMENTO DE ADN QUE CODIFICA UN POLIPEPTIDO ADECUADO. REUNIR Y MONTAR LOS FRAGMENTOS PARA FORMAR UN VECTOR DE EXPRESION BAJO EL CONTROL DEL PROMOTORY TRANSFORMAR EL CULTIVO DE CELULAS DE VERTEBRADOS CON EL VECTOR.ESTOS ANTIGENOS TIENEN APLICACIONES PARA LA PREPARACION DE VACUNAS CONTRA VIRUS DE HEPATITIS B.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US29823581A | 1981-08-31 | 1981-08-31 | |
US32698081A | 1981-12-03 | 1981-12-03 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES515260A0 ES515260A0 (es) | 1983-12-01 |
ES8401134A1 true ES8401134A1 (es) | 1983-12-01 |
Family
ID=26970555
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES515260A Expired ES8401134A1 (es) | 1981-08-31 | 1982-08-26 | "metodo de producir un cultivo transformante de celulas de vertebrado". |
Country Status (17)
Country | Link |
---|---|
EP (2) | EP0073656B1 (es) |
JP (3) | JPS5856685A (es) |
AU (2) | AU555575B2 (es) |
BR (1) | BR8205078A (es) |
DE (1) | DE3280310D1 (es) |
DK (1) | DK173592B1 (es) |
ES (1) | ES8401134A1 (es) |
GB (1) | GB2105344B (es) |
GR (1) | GR76906B (es) |
HK (1) | HK29689A (es) |
HU (1) | HU196238B (es) |
IE (1) | IE53873B1 (es) |
IL (1) | IL66637A (es) |
KE (1) | KE3838A (es) |
NO (1) | NO165598C (es) |
NZ (1) | NZ201706A (es) |
ZW (1) | ZW18282A1 (es) |
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5196194A (en) * | 1979-05-24 | 1993-03-23 | The Regents Of The University Of California | Vaccines containing Hepatitis B S-protein |
US4769238A (en) | 1981-08-04 | 1988-09-06 | The Regents Of The University Of California | Synthesis of human virus antigens by yeast |
ZW18282A1 (en) * | 1981-08-31 | 1983-03-23 | Genentech Inc | Preparation of polypeptides in vertebrate cell culture |
JPS5936698A (ja) * | 1982-08-20 | 1984-02-28 | Science & Tech Agency | B型肝炎ウイルス遺伝子を組込んだ組換えdnaおよび形質転換動物細胞 |
CA1209501A (en) * | 1982-09-16 | 1986-08-12 | Nikos Panayotatos | Expression vector |
US4820642A (en) * | 1983-04-04 | 1989-04-11 | The Regents Of The University Of California | Amplified expression vector |
US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
US7264817B1 (en) | 1983-08-30 | 2007-09-04 | Genentech, Inc. | Immunogenic composition based on a truncated derivative of a membrane bound protein and process for making it |
NZ209308A (en) | 1983-08-30 | 1991-08-27 | Genentech Inc | Vaccine against hsv involving a truncated membrane-free derivative of a membrane-bound protein |
WO1985001516A1 (fr) * | 1983-10-03 | 1985-04-11 | Transgene S.A. | Vecteurs d'expression d'une proteine antigenique de la rage dans les cellules eucaryotes et leur application a la preparation d'un vaccin |
FR2552776B1 (fr) * | 1983-10-03 | 1986-05-09 | Transgene Sa | Vecteurs d'expression d'une proteine antigenique de la rage dans les cellules eucaryotes et leur application a la preparation d'un vaccin |
JPS6078999A (ja) * | 1983-10-05 | 1985-05-04 | Chemo Sero Therapeut Res Inst | HBs抗原の精製方法 |
US5639639A (en) * | 1983-11-02 | 1997-06-17 | Genzyme Corporation | Recombinant heterodimeric human fertility hormones, and methods, cells, vectors and DNA for the production thereof |
US5118627A (en) * | 1984-02-27 | 1992-06-02 | Amgen | Papova virus construction |
FR2560890B1 (fr) * | 1984-03-07 | 1987-10-16 | Grp Genie Genetique | Composition utile pour la fabrication de vaccins contenant des particules portant l'antigene de surface du virus de l'hepatite b et le recepteur de l'albumine serique humaine polymerisee, cellules animales capables de produire de telles particules et procede pour leur obtention |
US4777240A (en) * | 1984-03-08 | 1988-10-11 | Scripps Clinic And Research Foundation | SV40 expression vector containing HBxAg as an expression marker |
US4855224A (en) * | 1984-03-09 | 1989-08-08 | Genentech, Inc. | Molecularly cloned diagnostic product and method of use |
US4663281A (en) * | 1984-03-22 | 1987-05-05 | Mass Institute Of Technology | Enhanced production of proteinaceous materials in eucaryotic cells |
FR2564106B1 (fr) * | 1984-05-09 | 1988-04-22 | Transgene Sa | Vecteurs d'expression du facteur ix, cellules transformees par ces vecteurs et procede de preparation du facteur ix. |
US5683688A (en) * | 1984-05-31 | 1997-11-04 | Genentech, Inc. | Unglycosylated recombinant human lymphotoxin polypeptides and compositions |
WO1986006405A1 (en) * | 1985-05-02 | 1986-11-06 | Takeda Chemical Industries, Ltd. | Novel transformant and its use |
EP0213628A3 (en) * | 1985-09-03 | 1988-09-21 | Yeda Research And Development Company, Ltd. | Expression of superoxide dismutase in eukaryotic cells |
SE8505027D0 (sv) * | 1985-10-24 | 1985-10-24 | Kabigen Ab | A lytic virulent phage, a hostcell containing same and a process for protein production |
EP0228036A3 (en) * | 1985-12-18 | 1988-09-07 | Microgenesys, Inc. | Method for producing selected polypeptides in virally infected insect cells and polypeptides isolated therefrom |
AU582288B2 (en) * | 1986-03-07 | 1989-03-16 | Damon Biotech Inc. | Vector and method for achieving high level expression in eukaryotic cells |
US5004693A (en) * | 1986-07-18 | 1991-04-02 | E. I. Du Pont De Nemours And Company | Pseudorabies virus recombinants and their use in the production of proteins |
NZ219516A (en) * | 1987-02-10 | 1991-02-26 | Wellcome Found | Fusion proteins of hbcag (hepatitis b virus core antigen) |
US5024939A (en) * | 1987-07-09 | 1991-06-18 | Genentech, Inc. | Transient expression system for producing recombinant protein |
US5578468A (en) * | 1987-08-10 | 1996-11-26 | Duke University | Site-specific RNA cleavage |
US5443964A (en) * | 1987-08-10 | 1995-08-22 | Duke University | Poxvirus insertion/expression vector |
JPH01285196A (ja) * | 1988-05-11 | 1989-11-16 | Chisso Corp | 発光蛋白エクオリンの製造法 |
US5709995A (en) | 1994-03-17 | 1998-01-20 | The Scripps Research Institute | Hepatitis C virus-derived peptides capable of inducing cytotoxic T lymphocyte responses |
JP4085231B2 (ja) * | 2000-02-28 | 2008-05-14 | 株式会社ビークル | タンパク質中空ナノ粒子とそれを用いた物質運搬体、ならびに細胞への物質導入方法 |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3636191A (en) * | 1969-10-08 | 1972-01-18 | Cancer Res Inst | Vaccine against viral hepatitis and process |
US4164565A (en) * | 1975-03-14 | 1979-08-14 | New York Blood Center, Inc. | Vaccine for active immunization containing hepatitis B surface antigen and associated antigen |
FR2444713A1 (fr) | 1978-12-18 | 1980-07-18 | Pasteur Institut | Procede de production d'un adn comprenant le genome du virus de l'hepatite b et vecteur le comportant |
FR2480779B2 (fr) * | 1979-08-30 | 1986-07-18 | Anvar | Vecteur contenant une sequence nucleotidique de l'antigene de surface du virus de l'hepatite b et procede de fabrication d'une molecule immunogene mettant en oeuvre ce vecteur |
ES8105035A1 (es) * | 1978-12-22 | 1981-05-16 | Biogen Nv | Un metodo para producir al menos un polipeptido que muestra antigenicidad de hbv |
IE52036B1 (en) | 1979-05-24 | 1987-05-27 | Univ California | Non-passageable viruses |
JPS5610119A (en) * | 1979-07-05 | 1981-02-02 | Alpha Therapeutic Corp | Manufacture of b type hepatitis infection preventive vaccine |
US4399216A (en) * | 1980-02-25 | 1983-08-16 | The Trustees Of Columbia University | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
ZA811895B (en) * | 1980-04-07 | 1982-04-28 | Univ California | Expression of hormone genomic clones |
FR2480780B1 (fr) * | 1980-04-22 | 1985-12-06 | Pasteur Institut | Procede de transformation de cellules, notamment eucaryotes, par un adn circulaire tel que celui du virus de l'hepatite b et preparations contenant les produits d'expression desdits adn |
EP0038765B1 (fr) * | 1980-04-22 | 1987-09-02 | Institut Pasteur | Vaccin contre l'hépatite virale B, procédé et cellules eucaryotes transformées pour la production de ce vaccin |
EP0062574A3 (en) * | 1981-03-31 | 1982-12-29 | The Regents Of The University Of California | Virus protein synthesis |
JPS5813598A (ja) * | 1981-06-22 | 1983-01-26 | イーライ・リリー・アンド・カンパニー | 組み替えdnaクロ−ニング・ベクタ−ならびにその真核細胞性および原核細胞性被形質転換体 |
ZW18282A1 (en) | 1981-08-31 | 1983-03-23 | Genentech Inc | Preparation of polypeptides in vertebrate cell culture |
NZ201705A (en) * | 1981-08-31 | 1986-03-14 | Genentech Inc | Recombinant dna method for production of hepatitis b surface antigen in yeast |
US4442205A (en) * | 1981-09-22 | 1984-04-10 | The United States Of America As Represented By The Department Of Health And Human Services | Simian virus recombinant that directs the synthesis of hepatitis B surface antigen |
AU561343B2 (en) * | 1981-10-19 | 1987-05-07 | Genentech Inc. | Human immune interferon by recombinant dna |
FR2521165A1 (fr) * | 1982-02-08 | 1983-08-12 | Pasteur Institut | Fragments d'adn codant pour un peptide immunogene susceptible d'introduire in vivo la synthese d'anticorps anti-poliovirus |
US4446235A (en) * | 1982-03-22 | 1984-05-01 | Genentech, Inc. | Method for cloning human growth hormone varient genes |
-
1982
- 1982-08-06 ZW ZW182/82A patent/ZW18282A1/xx unknown
- 1982-08-25 IL IL66637A patent/IL66637A/xx not_active IP Right Cessation
- 1982-08-25 NZ NZ201706A patent/NZ201706A/en unknown
- 1982-08-26 EP EP82304512A patent/EP0073656B1/en not_active Revoked
- 1982-08-26 EP EP87202618A patent/EP0291586A3/en not_active Withdrawn
- 1982-08-26 ES ES515260A patent/ES8401134A1/es not_active Expired
- 1982-08-26 DE DE8282304512T patent/DE3280310D1/de not_active Revoked
- 1982-08-26 GB GB08224527A patent/GB2105344B/en not_active Expired
- 1982-08-27 IE IE2084/82A patent/IE53873B1/en not_active IP Right Cessation
- 1982-08-27 AU AU87798/82A patent/AU555575B2/en not_active Expired
- 1982-08-30 JP JP57150627A patent/JPS5856685A/ja active Granted
- 1982-08-30 HU HU822777A patent/HU196238B/hu unknown
- 1982-08-30 BR BR8205078A patent/BR8205078A/pt unknown
- 1982-08-30 NO NO822926A patent/NO165598C/no not_active IP Right Cessation
- 1982-08-30 DK DK198203869A patent/DK173592B1/da not_active IP Right Cessation
- 1982-08-30 GR GR69154A patent/GR76906B/el unknown
-
1986
- 1986-08-20 AU AU61671/86A patent/AU589673B2/en not_active Expired
-
1988
- 1988-10-25 KE KE3838A patent/KE3838A/xx unknown
-
1989
- 1989-04-06 HK HK296/89A patent/HK29689A/xx not_active IP Right Cessation
-
1993
- 1993-07-30 JP JP5189904A patent/JP2634371B2/ja not_active Expired - Lifetime
-
1995
- 1995-08-07 JP JP7201034A patent/JP2726641B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DK386982A (da) | 1983-03-01 |
JP2634371B2 (ja) | 1997-07-23 |
JPS5856685A (ja) | 1983-04-04 |
AU589673B2 (en) | 1989-10-19 |
EP0291586A2 (en) | 1988-11-23 |
IL66637A (en) | 1991-11-21 |
BR8205078A (pt) | 1983-08-09 |
ES515260A0 (es) | 1983-12-01 |
HK29689A (en) | 1989-04-14 |
JPH0587518B2 (es) | 1993-12-16 |
NO822926L (no) | 1983-03-01 |
GB2105344A (en) | 1983-03-23 |
EP0073656A3 (en) | 1984-10-24 |
ZW18282A1 (en) | 1983-03-23 |
AU8779882A (en) | 1983-03-10 |
AU6167186A (en) | 1987-01-08 |
KE3838A (en) | 1988-12-02 |
IL66637A0 (en) | 1982-12-31 |
DE3280310D1 (de) | 1991-04-11 |
GB2105344B (en) | 1985-10-09 |
NZ201706A (en) | 1986-05-09 |
GR76906B (es) | 1984-09-04 |
NO165598B (no) | 1990-11-26 |
HU196238B (en) | 1988-10-28 |
EP0291586A3 (en) | 1988-12-07 |
NO165598C (no) | 1991-03-06 |
DK173592B1 (da) | 2001-04-09 |
EP0073656B1 (en) | 1991-03-06 |
IE822084L (en) | 1983-02-28 |
JPH0937777A (ja) | 1997-02-10 |
JPH06261748A (ja) | 1994-09-20 |
JP2726641B2 (ja) | 1998-03-11 |
AU555575B2 (en) | 1986-10-02 |
IE53873B1 (en) | 1989-03-29 |
EP0073656A2 (en) | 1983-03-09 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 20030903 |