KR850003163A - 완전변이 표현 콘트롤 시스템 - Google Patents

완전변이 표현 콘트롤 시스템 Download PDF

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Publication number
KR850003163A
KR850003163A KR1019840006086A KR840006086A KR850003163A KR 850003163 A KR850003163 A KR 850003163A KR 1019840006086 A KR1019840006086 A KR 1019840006086A KR 840006086 A KR840006086 A KR 840006086A KR 850003163 A KR850003163 A KR 850003163A
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South Korea
Prior art keywords
protein
promoter
dna sequence
control system
host
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KR1019840006086A
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English (en)
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루이스 헤이네커 허버트 (외 2)
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월터 에이취. 드레거
제넨테크 인코포레이티드
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Application filed by 월터 에이취. 드레거, 제넨테크 인코포레이티드 filed Critical 월터 에이취. 드레거
Publication of KR850003163A publication Critical patent/KR850003163A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon

Abstract

내용 없음

Description

완전변이 표현 콘트롤 시스템
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명의 콘트롤 시스템을 함유한 표현 플라스미드의 재조합 다이아그램임.

Claims (11)

  1. 원핵 숙주에 이종인 단백질을 암호화시키는 유전자에 접합된 프로모터/오퍼레이터로 되는 제1 DNA서열과, 프로모터/오퍼레이터와 공존할 수 있는 리프레서 단백질을 암호화시키고 숙주에 보완성을 갖는 유전자에 접합된 유도성 프로모터로 되는 제2DNA 서열(상기 제1DNA 서열과 제2DNA 서열이 숙주에 대하여 변이성임)시스템으로 되는 원핵숙주에 이종인 단백질을 암호화시키는 유전자의 표현을 억제시킬수 있는 콘트롤 시스템.
  2. 제1항에 있어서, 제1 DNA 서열이 trp 프로모터/오퍼레이터로 되는 콘트롤 시스템.
  3. 제1항에 있어서, 제2 DNA 서열이 rlacR 프로모터로 되는 콘트롤 시스템.
  4. 제2항에 있어서, 제DNA서열이 trpR 유전자에 접합된 프로모터로 되는 콘트롤 시스템.
  5. 제1항에 있어서, 숙주가 대장균 균주인 콘트롤 시스템.
  6. 제1항에 있어서, 이종 단백질을 FMD항원 단백질, 포유동물 성장호르몬, 포유동물 인터페론, 인체조직 플라스미노겐 활성인자, 유로키나제 및 IL-2로 되는 군중에서 선택하는 콘트롤 시스템.
  7. 제1항의 콘트롤 시스템에 있어서, 상기 숙주에 이종인 소기의 단백질을 암호화시키는 유전자를 표현시킴에 있어서 원핵 숙주 세포에 유효한 표현 벡터.
  8. 제7항의 표현 벡터로 형질변이시킨 세포 또는 세포 배양체.
  9. 제7항의 표현 벡터에 배치된 이종 단백질을 암호화시키는 유전자의 표현에 의해 성성된 단백질.
  10. 제8항의 세포를 배양시켜서 이종 단백질을 암호화시키는 유전자의 표현을 조절시키는 방법.
  11. 이종 단백질을 암호화시키는 유전자에 접합된 프로모터/오퍼레이터로 되는 제1 DNA 서열과, 프로모터/ 오퍼레이터와 공존할 수 있는 리프테서 단백질을 암호화시키고 숙주에 보완성을 갖는 유전자에 접합된 유도성 프로모터로 되는 제2 DNA 서열(상기 제1DNA 서열과 제2 DNA 서열이 숙주에 대하여 변이성임)로 되는 유전자용 표현벡터로 형질변이시킨 세포를 배양시켜 소지의 이종 단백질을 암호화시키는 유전자의 표현을 조절시키는 방법.
    ※참고사항:최초출원 내용에 의하여 공개하는 것임.
KR1019840006086A 1983-10-03 1984-10-02 완전변이 표현 콘트롤 시스템 KR850003163A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53873083A 1983-10-03 1983-10-03
US06/538730 1983-10-03

Publications (1)

Publication Number Publication Date
KR850003163A true KR850003163A (ko) 1985-06-13

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KR1019840006086A KR850003163A (ko) 1983-10-03 1984-10-02 완전변이 표현 콘트롤 시스템

Country Status (9)

Country Link
EP (1) EP0136907A3 (ko)
JP (1) JPS60156390A (ko)
KR (1) KR850003163A (ko)
AU (1) AU3375984A (ko)
DK (1) DK472084A (ko)
ES (1) ES8602138A1 (ko)
GR (1) GR80521B (ko)
IL (1) IL73149A0 (ko)
ZA (1) ZA847742B (ko)

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Also Published As

Publication number Publication date
ZA847742B (en) 1985-05-29
IL73149A0 (en) 1985-01-31
DK472084D0 (da) 1984-10-02
DK472084A (da) 1985-05-07
EP0136907A3 (en) 1986-12-30
JPS60156390A (ja) 1985-08-16
GR80521B (en) 1985-02-04
EP0136907A2 (en) 1985-04-10
ES536492A0 (es) 1985-12-01
ES8602138A1 (es) 1985-12-01
AU3375984A (en) 1985-04-18

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