ES8400877A1 - Procedimiento de preparacion de hb1ag (antigeno superficial de la hepatitis b). - Google Patents
Procedimiento de preparacion de hb1ag (antigeno superficial de la hepatitis b).Info
- Publication number
- ES8400877A1 ES8400877A1 ES514712A ES514712A ES8400877A1 ES 8400877 A1 ES8400877 A1 ES 8400877A1 ES 514712 A ES514712 A ES 514712A ES 514712 A ES514712 A ES 514712A ES 8400877 A1 ES8400877 A1 ES 8400877A1
- Authority
- ES
- Spain
- Prior art keywords
- yeast
- hbsag
- synthesis
- purified
- particles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 8
- 230000015572 biosynthetic process Effects 0.000 title abstract 4
- 238000003786 synthesis reaction Methods 0.000 title abstract 4
- 241000700605 Viruses Species 0.000 title 1
- 239000000427 antigen Substances 0.000 title 1
- 102000036639 antigens Human genes 0.000 title 1
- 108091007433 antigens Proteins 0.000 title 1
- 239000002245 particle Substances 0.000 abstract 3
- 239000013604 expression vector Substances 0.000 abstract 2
- 238000004062 sedimentation Methods 0.000 abstract 2
- 102100031673 Corneodesmosin Human genes 0.000 abstract 1
- 229940096437 Protein S Drugs 0.000 abstract 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract 1
- 229930006000 Sucrose Natural products 0.000 abstract 1
- 108010031318 Vitronectin Proteins 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 230000000890 antigenic effect Effects 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 239000005720 sucrose Substances 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2730/00—Reverse transcribing DNA viruses
- C12N2730/00011—Details
- C12N2730/10011—Hepadnaviridae
- C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
- C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Virology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Saccharide Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
SINTESIS DE HBSAG (ANTIGENO SUPERFICIAL DE LA HEPATITIS B) EN LEVADURAS.SE CULTIVAN CELULAS DE LEVADURA QUE HAN SIDO TRANSFORMADAS POR UN VECTOR DE TRANSFERENCIA DE DNA QUE COMPRENDE UN SEGMENTO QUE CODIFICA PARA EL ANTIGENO SUPERFICIAL DE LA HEPATITIS B. SE PREPARA UN EXTRACTO DE LAS CELULAS DE LEVADURA. SE PURIFICA EL ANTIGENO SUPERFICIAL DE LA HEPATITIS B DEL EXTRACTO. OPCIONALMENTE SE PREPARA UNA VACUNA POR ADICION DEL ANTIGENO SUPERFICIAL DE LA HEPATITIS B SINTETIZADO POR LA LEVADURA A UN MEDIO FISIOLOGICAMENTE ACEPTABLE. TAMBIEN PUEDEN PREPARARSE ANTICUERPOSCONTRA EL ANTIGENO SUPERFICIAL DE LA HEPATITIS B SINTETIZADO POR LA LEVADURA, POR INMUNIZACION DE UN ANIMAL CON EL ANTIGENO SUPERFICIAL DE LA HEPATITIS B SINTETIZADO POR LA LEVADURA Y PURIFICACION DEL ANTICUERPO PROCEDENTE DEL SUERO DEL ANIMAL.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US28991581A | 1981-08-04 | 1981-08-04 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES8400877A1 true ES8400877A1 (es) | 1983-11-16 |
ES514712A0 ES514712A0 (es) | 1983-11-16 |
Family
ID=23113710
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES514712A Granted ES514712A0 (es) | 1981-08-04 | 1982-08-03 | Procedimiento de preparacion de hb1ag (antigeno superficial de la hepatitis b). |
Country Status (12)
Country | Link |
---|---|
EP (1) | EP0072318B1 (es) |
JP (1) | JPS5877823A (es) |
AT (1) | ATE123225T1 (es) |
CA (1) | CA1341642C (es) |
DE (2) | DE3280186D1 (es) |
DK (2) | DK163931C (es) |
ES (1) | ES514712A0 (es) |
GR (1) | GR76274B (es) |
HK (1) | HK64591A (es) |
IE (1) | IE65996B1 (es) |
PT (1) | PT75374B (es) |
SG (1) | SG58191G (es) |
Families Citing this family (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NZ201705A (en) * | 1981-08-31 | 1986-03-14 | Genentech Inc | Recombinant dna method for production of hepatitis b surface antigen in yeast |
JPS58146281A (ja) * | 1981-10-19 | 1983-08-31 | Suntory Ltd | 酵母細胞の形質転換法 |
EP0105149B1 (en) * | 1982-08-16 | 1990-05-16 | Science and Technology Agency, Minister's Secretariat, Director of Finance Division | Recombinant plasmid containing hepatitis b virus gene, yeast transformed with said recombinant plasmid, and production of hepatitis b virus surface antigen |
AU584580B2 (en) * | 1982-09-08 | 1989-06-01 | Smith Kline - Rit | Hepatitis B virus vaccine |
US4876197A (en) * | 1983-02-22 | 1989-10-24 | Chiron Corporation | Eukaryotic regulatable transcription |
CA1341302C (en) * | 1983-02-22 | 2001-10-09 | Rae Lyn Burke | Yeast expression systems with vectors having gapdh or pyk promoters and synthesis of foreign protein |
JPS6078999A (ja) * | 1983-10-05 | 1985-05-04 | Chemo Sero Therapeut Res Inst | HBs抗原の精製方法 |
JPS60104020A (ja) * | 1983-10-14 | 1985-06-08 | メルク エンド カムパニー インコーポレーテツド | A型肝炎‐タンパク質サブユニツト抗原 |
DE3587759T2 (de) * | 1984-05-11 | 1994-07-07 | Chiron Corp | Erhöhte Hefetranskription unter Verwendung einer Hybridkonstruktion der Promotorregion. |
DE3585578D1 (de) * | 1984-06-18 | 1992-04-16 | Chiron Corp | Hepatitisoberflaechenantigenpartikelvakzin. |
US4624918A (en) * | 1984-07-09 | 1986-11-25 | Genentech, Inc. | Purification process for hepatitis surface antigen and product thereof |
ATE70308T1 (de) * | 1984-09-12 | 1991-12-15 | Chiron Corp | Hybridpartikel-immunogene. |
US4722840A (en) * | 1984-09-12 | 1988-02-02 | Chiron Corporation | Hybrid particle immunogens |
DE3689899T2 (de) * | 1985-04-08 | 1994-09-15 | Amgen | Verfahren und hybridpromotor zur steuerung der exogenen gentranskription. |
US4649192A (en) * | 1985-05-30 | 1987-03-10 | Smith Kline-Rit | Method for the isolation and purification of hepatitis B surface antigen using polysorbate |
JPH07106141B2 (ja) * | 1985-08-15 | 1995-11-15 | アムジエン | 酵母細胞から外来遺伝子生成物の発現を増進させるための培地および方法 |
JP2810364B2 (ja) * | 1985-10-04 | 1998-10-15 | ジ・アップジョン・カンパニー | 偽狂犬病ウイルス蛋白質 |
US4935349A (en) * | 1986-01-17 | 1990-06-19 | Zymogenetics, Inc. | Expression of higher eucaryotic genes in aspergillus |
US4816564A (en) * | 1986-01-31 | 1989-03-28 | Merck & Co., Inc. | Method for producing hepatitis B virus proteins in yeast |
JPH0768267B2 (ja) * | 1986-06-05 | 1995-07-26 | 財団法人阪大微生物病研究会 | フラビウイルス抗原 |
JPH0789951B2 (ja) * | 1986-06-18 | 1995-10-04 | 財団法人阪大微生物病研究会 | 遺伝子発現産物の精製法 |
JPH089637B2 (ja) * | 1986-06-18 | 1996-01-31 | 財団法人阪大微生物病研究会 | B型肝炎ウイルス抗原とその製造法 |
IL79740A0 (en) * | 1986-08-17 | 1986-11-30 | Yeda Res & Dev | Hepatitis vaccine |
WO1988003562A1 (en) * | 1986-11-01 | 1988-05-19 | Oxford Gene Systems Limited | Particulate hybrid hiv antigens |
US5026828A (en) * | 1987-02-27 | 1991-06-25 | Merck & Co., Inc. | Method of purifying recombinant pres-1/S-2/S/S hepatitis B antigen from yeast |
ATE91304T1 (de) * | 1987-02-27 | 1993-07-15 | Merck & Co Inc | Verfahren zur herstellung des pres 1/s2/shepatitis-b-antigens aus hefe. |
IL86833A0 (en) | 1987-06-22 | 1988-11-30 | Medico Labs | Peptide containing immunogenic particles |
US5011915A (en) * | 1987-10-26 | 1991-04-30 | Merck & Co., Inc. | Process for purifying recombinant hepatitis antigens |
NZ228948A (en) * | 1988-05-13 | 1991-06-25 | Phillips Petroleum Co | Hbv particles containing s and pre-s 2 proteins and recombinant processes for their production in yeast cells |
EP0491077A1 (en) * | 1990-12-19 | 1992-06-24 | Medeva Holdings B.V. | A composition used as a therapeutic agent against chronic viral hepatic diseases |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2270892A1 (en) * | 1974-05-14 | 1975-12-12 | Inst Francais D Immunologie | Vaccines from attenuated viruses - which have been serially incubated with yeast cells, especially Saccharomyces cerevisiae |
YU44186B (en) * | 1978-12-22 | 1990-04-30 | Biogen Nv | Process for obtaining recombinant dnk molecules |
IE52036B1 (en) * | 1979-05-24 | 1987-05-27 | Univ California | Non-passageable viruses |
FR2480780B1 (fr) * | 1980-04-22 | 1985-12-06 | Pasteur Institut | Procede de transformation de cellules, notamment eucaryotes, par un adn circulaire tel que celui du virus de l'hepatite b et preparations contenant les produits d'expression desdits adn |
JPH0314840A (ja) * | 1988-12-28 | 1991-01-23 | Kureha Chem Ind Co Ltd | 弗化ビニリデン系ポリマー二軸冷延伸フィルムおよびその製造方法 |
-
1982
- 1982-08-02 GR GR68922A patent/GR76274B/el unknown
- 1982-08-03 PT PT75374A patent/PT75374B/pt unknown
- 1982-08-03 CA CA408658A patent/CA1341642C/en active Active
- 1982-08-03 ES ES514712A patent/ES514712A0/es active Granted
- 1982-08-03 DK DK347682A patent/DK163931C/da not_active IP Right Cessation
- 1982-08-04 EP EP82401473A patent/EP0072318B1/en not_active Expired - Lifetime
- 1982-08-04 JP JP57135279A patent/JPS5877823A/ja active Granted
- 1982-08-04 AT AT89110270T patent/ATE123225T1/de not_active IP Right Cessation
- 1982-08-04 DE DE8282401473T patent/DE3280186D1/de not_active Expired - Lifetime
- 1982-08-04 IE IE187582A patent/IE65996B1/en not_active IP Right Cessation
- 1982-08-04 DE DE3280468T patent/DE3280468T2/de not_active Expired - Lifetime
-
1991
- 1991-06-14 DK DK114791A patent/DK169152B1/da not_active IP Right Cessation
- 1991-07-19 SG SG581/91A patent/SG58191G/en unknown
- 1991-08-15 HK HK645/91A patent/HK64591A/xx not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
EP0072318B1 (en) | 1990-05-30 |
JPH0526798B2 (es) | 1993-04-19 |
DK114791A (da) | 1991-06-14 |
DK114791D0 (da) | 1991-06-14 |
EP0072318A3 (en) | 1983-03-16 |
DK169152B1 (da) | 1994-08-29 |
HK64591A (en) | 1991-08-23 |
GR76274B (es) | 1984-08-04 |
DK163931C (da) | 1992-09-14 |
PT75374B (en) | 1985-12-13 |
DK163931B (da) | 1992-04-21 |
EP0072318A2 (en) | 1983-02-16 |
SG58191G (en) | 1993-02-19 |
PT75374A (en) | 1982-09-01 |
ES514712A0 (es) | 1983-11-16 |
IE65996B1 (en) | 1995-11-29 |
CA1341642C (en) | 2023-02-21 |
JPS5877823A (ja) | 1983-05-11 |
DE3280468D1 (de) | 1995-07-06 |
DK347682A (da) | 1983-02-05 |
DE3280186D1 (de) | 1990-07-05 |
DE3280468T2 (de) | 1995-12-07 |
ATE123225T1 (de) | 1995-06-15 |
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