ES2954784T3 - Amplificación de ADN en fase de solución y en fase sólida combinadas - Google Patents
Amplificación de ADN en fase de solución y en fase sólida combinadas Download PDFInfo
- Publication number
- ES2954784T3 ES2954784T3 ES20725896T ES20725896T ES2954784T3 ES 2954784 T3 ES2954784 T3 ES 2954784T3 ES 20725896 T ES20725896 T ES 20725896T ES 20725896 T ES20725896 T ES 20725896T ES 2954784 T3 ES2954784 T3 ES 2954784T3
- Authority
- ES
- Spain
- Prior art keywords
- amplification
- immobilized
- nucleic acid
- primers
- chip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000007790 solid phase Substances 0.000 title claims abstract description 26
- 239000012071 phase Substances 0.000 title abstract description 43
- 230000004544 DNA amplification Effects 0.000 title description 6
- 230000003321 amplification Effects 0.000 claims abstract description 190
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 190
- 150000007523 nucleic acids Chemical group 0.000 claims abstract description 72
- 238000000034 method Methods 0.000 claims abstract description 45
- 238000006243 chemical reaction Methods 0.000 claims description 61
- 108020004707 nucleic acids Proteins 0.000 claims description 57
- 102000039446 nucleic acids Human genes 0.000 claims description 57
- 238000001514 detection method Methods 0.000 claims description 40
- 239000007788 liquid Substances 0.000 claims description 23
- 239000002773 nucleotide Substances 0.000 claims description 23
- 125000003729 nucleotide group Chemical group 0.000 claims description 23
- 239000000523 sample Substances 0.000 claims description 21
- 239000003153 chemical reaction reagent Substances 0.000 claims description 10
- 230000003287 optical effect Effects 0.000 claims description 6
- 238000004458 analytical method Methods 0.000 claims description 5
- 238000010348 incorporation Methods 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 3
- 229910052710 silicon Inorganic materials 0.000 claims description 3
- 239000010703 silicon Substances 0.000 claims description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims 1
- 239000003269 fluorescent indicator Substances 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 abstract description 11
- 239000000047 product Substances 0.000 description 56
- 239000000243 solution Substances 0.000 description 50
- 108091093088 Amplicon Proteins 0.000 description 35
- 108020004414 DNA Proteins 0.000 description 17
- 108090000790 Enzymes Proteins 0.000 description 11
- 102000004190 Enzymes Human genes 0.000 description 11
- 239000000872 buffer Substances 0.000 description 9
- 102000054765 polymorphisms of proteins Human genes 0.000 description 9
- 108091034117 Oligonucleotide Proteins 0.000 description 8
- 238000012790 confirmation Methods 0.000 description 8
- 239000000499 gel Substances 0.000 description 8
- 230000035772 mutation Effects 0.000 description 8
- 238000003556 assay Methods 0.000 description 7
- 230000000295 complement effect Effects 0.000 description 7
- 241000607715 Serratia marcescens Species 0.000 description 6
- 238000009396 hybridization Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 230000001717 pathogenic effect Effects 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000011897 real-time detection Methods 0.000 description 6
- 241000235347 Schizosaccharomyces pombe Species 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 238000001502 gel electrophoresis Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 206010040047 Sepsis Diseases 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 244000052769 pathogen Species 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 229920001213 Polysorbate 20 Polymers 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 230000002051 biphasic effect Effects 0.000 description 3
- 230000003139 buffering effect Effects 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000010530 solution phase reaction Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 239000012807 PCR reagent Substances 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000004880 explosion Methods 0.000 description 2
- 238000000799 fluorescence microscopy Methods 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 239000000017 hydrogel Substances 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- 239000012160 loading buffer Substances 0.000 description 2
- 238000011880 melting curve analysis Methods 0.000 description 2
- 238000002493 microarray Methods 0.000 description 2
- 238000012634 optical imaging Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 208000013223 septicemia Diseases 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000003746 solid phase reaction Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 208000035049 Blood-Borne Infections Diseases 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000495778 Escherichia faecalis Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 206010029719 Nonspecific reaction Diseases 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 108091036333 Rapid DNA Proteins 0.000 description 1
- 206010040070 Septic Shock Diseases 0.000 description 1
- 101000794822 Serratia marcescens Anthranilate synthase component 1 Proteins 0.000 description 1
- 101000847781 Serratia marcescens Anthranilate synthase component 2 Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 238000007846 asymmetric PCR Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009640 blood culture Methods 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-N dCTP Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO[P@](O)(=O)O[P@](O)(=O)OP(O)(O)=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-N 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 231100000676 disease causative agent Toxicity 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000003366 endpoint assay Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000005669 field effect Effects 0.000 description 1
- 238000012632 fluorescent imaging Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 1
- 239000011654 magnesium acetate Substances 0.000 description 1
- 229940069446 magnesium acetate Drugs 0.000 description 1
- 235000011285 magnesium acetate Nutrition 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 201000004792 malaria Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000036303 septic shock Effects 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 239000006104 solid solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003239 susceptibility assay Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
- G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/118—Prognosis of disease development
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Electrochemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB201906461 | 2019-05-08 | ||
| PCT/GB2020/051122 WO2020225564A1 (en) | 2019-05-08 | 2020-05-07 | Combined solution phase and solid phase dna amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2954784T3 true ES2954784T3 (es) | 2023-11-24 |
Family
ID=67002052
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES20725896T Active ES2954784T3 (es) | 2019-05-08 | 2020-05-07 | Amplificación de ADN en fase de solución y en fase sólida combinadas |
| ES23185680T Active ES3023484T3 (en) | 2019-05-08 | 2020-05-07 | Combined solution phase and solid phase dna amplification |
| ES25150312T Active ES3052734T3 (en) | 2019-05-08 | 2020-05-07 | Combined solution phase and solid phase dna amplification |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES23185680T Active ES3023484T3 (en) | 2019-05-08 | 2020-05-07 | Combined solution phase and solid phase dna amplification |
| ES25150312T Active ES3052734T3 (en) | 2019-05-08 | 2020-05-07 | Combined solution phase and solid phase dna amplification |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20220333167A1 (https=) |
| EP (3) | EP3966346B1 (https=) |
| JP (2) | JP7611858B2 (https=) |
| CN (3) | CN119753103A (https=) |
| CA (2) | CA3139356C (https=) |
| ES (3) | ES2954784T3 (https=) |
| WO (1) | WO2020225564A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022104138A1 (en) * | 2020-11-14 | 2022-05-19 | Life Technologies Corporation | System and method for automated repeat sequencing |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6531302B1 (en) * | 1999-04-12 | 2003-03-11 | Nanogen/Becton Dickinson Partnership | Anchored strand displacement amplification on an electronically addressable microchip |
| US7575865B2 (en) * | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
| JP2009000070A (ja) * | 2007-06-22 | 2009-01-08 | Hitachi Ltd | 大規模並列核酸分析方法 |
| JP4853403B2 (ja) * | 2007-07-02 | 2012-01-11 | セイコーエプソン株式会社 | 画像読み取り装置 |
| WO2012042399A1 (en) | 2010-09-30 | 2012-04-05 | Nxp B.V. | Biosensor device and method |
| GB2500715A (en) | 2012-03-30 | 2013-10-02 | Gene Onyx Ltd | Single nucleotide polymorphism detection using an ISFET array |
| US9914968B2 (en) | 2012-09-26 | 2018-03-13 | Cepheid | Honeycomb tube |
| ES2640570T3 (es) * | 2012-10-18 | 2017-11-03 | F. Hoffmann-La Roche Ag | Ensayo de doble sonda para la detección de VHC |
| GB201407334D0 (en) * | 2014-04-25 | 2014-06-11 | Dna Electronics Ltd | Integrated nucleic acid test system, instrument and method |
| HK1243466A1 (zh) | 2014-11-05 | 2018-07-13 | 加州理工学院 | 生物体对药物的响应的微流体测量 |
| WO2018046689A1 (en) * | 2016-09-08 | 2018-03-15 | Danmarks Tekniske Universitet | A polymeric chip system and uses thereof |
-
2020
- 2020-05-07 EP EP20725896.3A patent/EP3966346B1/en active Active
- 2020-05-07 CN CN202510021594.2A patent/CN119753103A/zh active Pending
- 2020-05-07 JP JP2021565983A patent/JP7611858B2/ja active Active
- 2020-05-07 CN CN202080034226.7A patent/CN114207146B/zh active Active
- 2020-05-07 EP EP25150312.4A patent/EP4524260B1/en active Active
- 2020-05-07 CN CN202510020785.7A patent/CN119955908A/zh active Pending
- 2020-05-07 CA CA3139356A patent/CA3139356C/en active Active
- 2020-05-07 ES ES20725896T patent/ES2954784T3/es active Active
- 2020-05-07 EP EP23185680.8A patent/EP4293124B1/en active Active
- 2020-05-07 ES ES23185680T patent/ES3023484T3/es active Active
- 2020-05-07 US US17/609,553 patent/US20220333167A1/en active Pending
- 2020-05-07 ES ES25150312T patent/ES3052734T3/es active Active
- 2020-05-07 CA CA3251083A patent/CA3251083A1/en active Pending
- 2020-05-07 WO PCT/GB2020/051122 patent/WO2020225564A1/en not_active Ceased
-
2024
- 2024-12-24 JP JP2024226980A patent/JP2025060794A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3966346B1 (en) | 2023-08-23 |
| CN114207146A (zh) | 2022-03-18 |
| CA3139356A1 (en) | 2020-11-12 |
| EP4524260A2 (en) | 2025-03-19 |
| ES3052734T3 (en) | 2026-01-13 |
| EP3966346A1 (en) | 2022-03-16 |
| EP4293124B1 (en) | 2025-02-19 |
| JP2025060794A (ja) | 2025-04-10 |
| CA3251083A1 (en) | 2025-10-30 |
| EP4293124A2 (en) | 2023-12-20 |
| CA3139356C (en) | 2025-04-01 |
| EP4524260A3 (en) | 2025-05-14 |
| EP4293124A3 (en) | 2024-01-17 |
| JP2022531707A (ja) | 2022-07-08 |
| CN119955908A (zh) | 2025-05-09 |
| CN119753103A (zh) | 2025-04-04 |
| WO2020225564A1 (en) | 2020-11-12 |
| US20220333167A1 (en) | 2022-10-20 |
| JP7611858B2 (ja) | 2025-01-10 |
| EP4524260B1 (en) | 2025-10-22 |
| CN114207146B (zh) | 2025-01-10 |
| ES3023484T3 (en) | 2025-06-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2273371T3 (es) | Metodo de amplificacion de acido nucleico basado en ramificacion extension (rami) y transcripcion in vitro. | |
| CN101003836B (zh) | 引物的设计方法、目标核酸的检测方法、单碱基突变的检测方法以及检测试剂盒 | |
| US8673595B2 (en) | Sample analysis method and assay kit used therein | |
| US20100105575A1 (en) | Single nucleotide polymorphism genotyping detection via the real-time invader assay microarray platform | |
| JP4256291B2 (ja) | 標的核酸配列の検出方法 | |
| ES2920954T3 (es) | Detección de ácido nucleico en superficie con un dispositivo de flujo de convección de fluidos | |
| JP2025060794A (ja) | 液相及び固相ｄｎａ増幅の組合せ | |
| JP6339787B2 (ja) | 核酸の分析方法 | |
| CN1810989B (zh) | 一种等温反应检测具序列特异性的dna和rna的方法 | |
| JP4381457B2 (ja) | 標的核酸配列の検出方法 | |
| HK40124729A (en) | Combined solution phase and solid phase dna amplification | |
| HK40105256B (en) | Combined solution phase and solid phase dna amplification | |
| US20060252058A1 (en) | Chip for detection of nucleic acid | |
| HK40070545B (en) | Combined solution phase and solid phase dna amplification | |
| HK40070545A (en) | Combined solution phase and solid phase dna amplification | |
| HK40105256A (en) | Combined solution phase and solid phase dna amplification | |
| KR101566402B1 (ko) | 마이크로어레이 칩을 이용한 흰반점바이러스 진단용 멀티플렉스 키트 | |
| RU114320U1 (ru) | УСТРОЙСТВО ДЛЯ ПРЯМОЙ ЭЛЕКТРОХИМИЧЕСКОЙ РЕГИСТРАЦИИ НЕПРАЙМИРОВАННОГО СИНТЕЗА МОЛЕКУЛ ДНК НА ОСНОВЕ pН-ЧУВСТВИТЕЛЬНОГО СЕНСОРА | |
| JPWO2016129609A1 (ja) | 標的核酸の検出方法 | |
| CN103589804B (zh) | 环介导等温扩增与核酸杂交的同步反应方法 | |
| JP2023113514A (ja) | ＳＡＲＳ－ＣｏＶ－２のオミクロン株を検出するオリゴヌクレオチド | |
| KR20140000734A (ko) | 비결합성 변형염기가 포함된 ｄｎａ 라이게이션 단편을 사용한 고효율의 리가아제 기반 ｓｎｐ분석 | |
| US20160017411A1 (en) | Assay for detecting a nucleic acid analyte in a biological sample | |
| JP2002000300A (ja) | 簡易塩基配列確認法 |