JP7611858B2 - 液相及び固相ｄｎａ増幅の組合せ - Google Patents

液相及び固相ｄｎａ増幅の組合せ Download PDF

Info

Publication number
JP7611858B2
JP7611858B2 JP2021565983A JP2021565983A JP7611858B2 JP 7611858 B2 JP7611858 B2 JP 7611858B2 JP 2021565983 A JP2021565983 A JP 2021565983A JP 2021565983 A JP2021565983 A JP 2021565983A JP 7611858 B2 JP7611858 B2 JP 7611858B2
Authority
JP
Japan
Prior art keywords
amplification
immobilized
nucleic acid
primer
primers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2021565983A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2020225564A5 (https=
JP2022531707A (ja
JP2022531707A5 (https=
Inventor
アラン デイヴィッドソン、デイヴィッド
ウォースリー、グレアム
キルパック、ジャレット
リード、サミュエル
Original Assignee
ディーエヌエイイー ダイアグノスティックス リミテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ディーエヌエイイー ダイアグノスティックス リミテッド filed Critical ディーエヌエイイー ダイアグノスティックス リミテッド
Publication of JP2022531707A publication Critical patent/JP2022531707A/ja
Publication of JPWO2020225564A5 publication Critical patent/JPWO2020225564A5/ja
Publication of JP2022531707A5 publication Critical patent/JP2022531707A5/ja
Priority to JP2024226980A priority Critical patent/JP2025060794A/ja
Application granted granted Critical
Publication of JP7611858B2 publication Critical patent/JP7611858B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/118—Prognosis of disease development
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Electrochemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2021565983A 2019-05-08 2020-05-07 液相及び固相ｄｎａ増幅の組合せ Active JP7611858B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2024226980A JP2025060794A (ja) 2019-05-08 2024-12-24 液相及び固相ｄｎａ増幅の組合せ

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB201906461 2019-05-08
GB1906461.7 2019-05-08
PCT/GB2020/051122 WO2020225564A1 (en) 2019-05-08 2020-05-07 Combined solution phase and solid phase dna amplification

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2024226980A Division JP2025060794A (ja) 2019-05-08 2024-12-24 液相及び固相ｄｎａ増幅の組合せ

Publications (4)

Publication Number Publication Date
JP2022531707A JP2022531707A (ja) 2022-07-08
JPWO2020225564A5 JPWO2020225564A5 (https=) 2023-03-01
JP2022531707A5 JP2022531707A5 (https=) 2023-03-01
JP7611858B2 true JP7611858B2 (ja) 2025-01-10

Family

ID=67002052

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2021565983A Active JP7611858B2 (ja) 2019-05-08 2020-05-07 液相及び固相ｄｎａ増幅の組合せ
JP2024226980A Pending JP2025060794A (ja) 2019-05-08 2024-12-24 液相及び固相ｄｎａ増幅の組合せ

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2024226980A Pending JP2025060794A (ja) 2019-05-08 2024-12-24 液相及び固相ｄｎａ増幅の組合せ

Country Status (7)

Country Link
US (1) US20220333167A1 (https=)
EP (3) EP3966346B1 (https=)
JP (2) JP7611858B2 (https=)
CN (3) CN119753103A (https=)
CA (2) CA3139356C (https=)
ES (3) ES2954784T3 (https=)
WO (1) WO2020225564A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022104138A1 (en) * 2020-11-14 2022-05-19 Life Technologies Corporation System and method for automated repeat sequencing

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012042399A1 (en) 2010-09-30 2012-04-05 Nxp B.V. Biosensor device and method
WO2013144580A1 (en) 2012-03-30 2013-10-03 Gene Onyx Limited Isfet array for detecting a single nucleotide polymorphism
JP2015532094A (ja) 2012-09-26 2015-11-09 セフェィド ハニカムチューブ
JP2017522858A (ja) 2014-04-25 2017-08-17 ディーエヌエーイー グループ ホールディングス エルティーディー 核酸検査のシステム、装置、及び方法
JP2018500926A (ja) 2014-11-05 2018-01-18 カリフォルニア インスティテュート オブ テクノロジー 薬剤に対する生物の応答のマイクロ流体測定

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6531302B1 (en) * 1999-04-12 2003-03-11 Nanogen/Becton Dickinson Partnership Anchored strand displacement amplification on an electronically addressable microchip
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
JP2009000070A (ja) * 2007-06-22 2009-01-08 Hitachi Ltd 大規模並列核酸分析方法
JP4853403B2 (ja) * 2007-07-02 2012-01-11 セイコーエプソン株式会社 画像読み取り装置
ES2640570T3 (es) * 2012-10-18 2017-11-03 F. Hoffmann-La Roche Ag Ensayo de doble sonda para la detección de VHC
WO2018046689A1 (en) * 2016-09-08 2018-03-15 Danmarks Tekniske Universitet A polymeric chip system and uses thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012042399A1 (en) 2010-09-30 2012-04-05 Nxp B.V. Biosensor device and method
WO2013144580A1 (en) 2012-03-30 2013-10-03 Gene Onyx Limited Isfet array for detecting a single nucleotide polymorphism
JP2015532094A (ja) 2012-09-26 2015-11-09 セフェィド ハニカムチューブ
JP2017522858A (ja) 2014-04-25 2017-08-17 ディーエヌエーイー グループ ホールディングス エルティーディー 核酸検査のシステム、装置、及び方法
JP2018500926A (ja) 2014-11-05 2018-01-18 カリフォルニア インスティテュート オブ テクノロジー 薬剤に対する生物の応答のマイクロ流体測定

Also Published As

Publication number Publication date
EP3966346B1 (en) 2023-08-23
CN114207146A (zh) 2022-03-18
CA3139356A1 (en) 2020-11-12
EP4524260A2 (en) 2025-03-19
ES3052734T3 (en) 2026-01-13
EP3966346A1 (en) 2022-03-16
EP4293124B1 (en) 2025-02-19
JP2025060794A (ja) 2025-04-10
CA3251083A1 (en) 2025-10-30
EP4293124A2 (en) 2023-12-20
CA3139356C (en) 2025-04-01
ES2954784T3 (es) 2023-11-24
EP4524260A3 (en) 2025-05-14
EP4293124A3 (en) 2024-01-17
JP2022531707A (ja) 2022-07-08
CN119955908A (zh) 2025-05-09
CN119753103A (zh) 2025-04-04
WO2020225564A1 (en) 2020-11-12
US20220333167A1 (en) 2022-10-20
EP4524260B1 (en) 2025-10-22
CN114207146B (zh) 2025-01-10
ES3023484T3 (en) 2025-06-02

Similar Documents

Publication Publication Date Title
US20100105575A1 (en) Single nucleotide polymorphism genotyping detection via the real-time invader assay microarray platform
JP4256291B2 (ja) 標的核酸配列の検出方法
IL261980B2 (en) Surface-based detection of nucleic acids in convection flow fluidic devices
JP2025060794A (ja) 液相及び固相ｄｎａ増幅の組合せ
CN1810989B (zh) 一种等温反应检测具序列特异性的dna和rna的方法
JP2014501494A (ja) 核酸標的の定量的多重同定
US20090275028A1 (en) Method of detecting target nucleic acid
JP4381457B2 (ja) 標的核酸配列の検出方法
US20240141420A1 (en) Parallel detection and quantification of nucleic acid based markers
HK40124729A (en) Combined solution phase and solid phase dna amplification
HK40105256B (en) Combined solution phase and solid phase dna amplification
HK40070545A (en) Combined solution phase and solid phase dna amplification
HK40070545B (en) Combined solution phase and solid phase dna amplification
HK40105256A (en) Combined solution phase and solid phase dna amplification
CN106916882B (zh) 用于辨识核苷酸基因多型性的基因型鉴定芯片的双重等位基因特异性聚合酶链锁反应的方法
JP6202455B2 (ja) 標的核酸の検出方法
JP4101810B2 (ja) マイクロアレイ再生方法及びマイクロアレイ再生試薬キット
EP4405497A1 (en) Nucleic acid detection
Kamau-Gatogo Development of RNA microchip for pathogen and cancer direct detection
WO2019100910A1 (zh) 基于多孔滤膜的核酸测序方法和装置
Ma et al. Multiple SNPs genotyping by ligation of universal probes on 3D DNA microarray

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230220

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20230220

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20240115

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20240123

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240415

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20240628

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240924

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20241204

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20241224

R150 Certificate of patent or registration of utility model

Ref document number: 7611858

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150