CN119753103A - 组合的液相和固相dna扩增 - Google Patents
组合的液相和固相dna扩增 Download PDFInfo
- Publication number
- CN119753103A CN119753103A CN202510021594.2A CN202510021594A CN119753103A CN 119753103 A CN119753103 A CN 119753103A CN 202510021594 A CN202510021594 A CN 202510021594A CN 119753103 A CN119753103 A CN 119753103A
- Authority
- CN
- China
- Prior art keywords
- amplification
- immobilized
- nucleic acid
- primers
- amplification product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
- G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/118—Prognosis of disease development
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Electrochemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB201906461 | 2019-05-08 | ||
| GB1906461.7 | 2019-05-08 | ||
| CN202080034226.7A CN114207146B (zh) | 2019-05-08 | 2020-05-07 | 组合的液相和固相dna扩增 |
| PCT/GB2020/051122 WO2020225564A1 (en) | 2019-05-08 | 2020-05-07 | Combined solution phase and solid phase dna amplification |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202080034226.7A Division CN114207146B (zh) | 2019-05-08 | 2020-05-07 | 组合的液相和固相dna扩增 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN119753103A true CN119753103A (zh) | 2025-04-04 |
Family
ID=67002052
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202510021594.2A Pending CN119753103A (zh) | 2019-05-08 | 2020-05-07 | 组合的液相和固相dna扩增 |
| CN202080034226.7A Active CN114207146B (zh) | 2019-05-08 | 2020-05-07 | 组合的液相和固相dna扩增 |
| CN202510020785.7A Pending CN119955908A (zh) | 2019-05-08 | 2020-05-07 | 组合的液相和固相dna扩增 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202080034226.7A Active CN114207146B (zh) | 2019-05-08 | 2020-05-07 | 组合的液相和固相dna扩增 |
| CN202510020785.7A Pending CN119955908A (zh) | 2019-05-08 | 2020-05-07 | 组合的液相和固相dna扩增 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20220333167A1 (https=) |
| EP (3) | EP3966346B1 (https=) |
| JP (2) | JP7611858B2 (https=) |
| CN (3) | CN119753103A (https=) |
| CA (2) | CA3139356C (https=) |
| ES (3) | ES2954784T3 (https=) |
| WO (1) | WO2020225564A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022104138A1 (en) * | 2020-11-14 | 2022-05-19 | Life Technologies Corporation | System and method for automated repeat sequencing |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6531302B1 (en) * | 1999-04-12 | 2003-03-11 | Nanogen/Becton Dickinson Partnership | Anchored strand displacement amplification on an electronically addressable microchip |
| US7575865B2 (en) * | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
| JP2009000070A (ja) * | 2007-06-22 | 2009-01-08 | Hitachi Ltd | 大規模並列核酸分析方法 |
| JP4853403B2 (ja) * | 2007-07-02 | 2012-01-11 | セイコーエプソン株式会社 | 画像読み取り装置 |
| WO2012042399A1 (en) | 2010-09-30 | 2012-04-05 | Nxp B.V. | Biosensor device and method |
| GB2500715A (en) | 2012-03-30 | 2013-10-02 | Gene Onyx Ltd | Single nucleotide polymorphism detection using an ISFET array |
| US9914968B2 (en) | 2012-09-26 | 2018-03-13 | Cepheid | Honeycomb tube |
| ES2640570T3 (es) * | 2012-10-18 | 2017-11-03 | F. Hoffmann-La Roche Ag | Ensayo de doble sonda para la detección de VHC |
| GB201407334D0 (en) * | 2014-04-25 | 2014-06-11 | Dna Electronics Ltd | Integrated nucleic acid test system, instrument and method |
| HK1243466A1 (zh) | 2014-11-05 | 2018-07-13 | 加州理工学院 | 生物体对药物的响应的微流体测量 |
| WO2018046689A1 (en) * | 2016-09-08 | 2018-03-15 | Danmarks Tekniske Universitet | A polymeric chip system and uses thereof |
-
2020
- 2020-05-07 EP EP20725896.3A patent/EP3966346B1/en active Active
- 2020-05-07 CN CN202510021594.2A patent/CN119753103A/zh active Pending
- 2020-05-07 JP JP2021565983A patent/JP7611858B2/ja active Active
- 2020-05-07 CN CN202080034226.7A patent/CN114207146B/zh active Active
- 2020-05-07 EP EP25150312.4A patent/EP4524260B1/en active Active
- 2020-05-07 CN CN202510020785.7A patent/CN119955908A/zh active Pending
- 2020-05-07 CA CA3139356A patent/CA3139356C/en active Active
- 2020-05-07 ES ES20725896T patent/ES2954784T3/es active Active
- 2020-05-07 EP EP23185680.8A patent/EP4293124B1/en active Active
- 2020-05-07 ES ES23185680T patent/ES3023484T3/es active Active
- 2020-05-07 US US17/609,553 patent/US20220333167A1/en active Pending
- 2020-05-07 ES ES25150312T patent/ES3052734T3/es active Active
- 2020-05-07 CA CA3251083A patent/CA3251083A1/en active Pending
- 2020-05-07 WO PCT/GB2020/051122 patent/WO2020225564A1/en not_active Ceased
-
2024
- 2024-12-24 JP JP2024226980A patent/JP2025060794A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3966346B1 (en) | 2023-08-23 |
| CN114207146A (zh) | 2022-03-18 |
| CA3139356A1 (en) | 2020-11-12 |
| EP4524260A2 (en) | 2025-03-19 |
| ES3052734T3 (en) | 2026-01-13 |
| EP3966346A1 (en) | 2022-03-16 |
| EP4293124B1 (en) | 2025-02-19 |
| JP2025060794A (ja) | 2025-04-10 |
| CA3251083A1 (en) | 2025-10-30 |
| EP4293124A2 (en) | 2023-12-20 |
| CA3139356C (en) | 2025-04-01 |
| ES2954784T3 (es) | 2023-11-24 |
| EP4524260A3 (en) | 2025-05-14 |
| EP4293124A3 (en) | 2024-01-17 |
| JP2022531707A (ja) | 2022-07-08 |
| CN119955908A (zh) | 2025-05-09 |
| WO2020225564A1 (en) | 2020-11-12 |
| US20220333167A1 (en) | 2022-10-20 |
| JP7611858B2 (ja) | 2025-01-10 |
| EP4524260B1 (en) | 2025-10-22 |
| CN114207146B (zh) | 2025-01-10 |
| ES3023484T3 (en) | 2025-06-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |