ES2906061T3 - Métodos para medir actividad enzimática, útiles para determinar la viabilidad celular en muestras no purificadas - Google Patents
Métodos para medir actividad enzimática, útiles para determinar la viabilidad celular en muestras no purificadas Download PDFInfo
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- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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Landscapes
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| EP3284831B1 (en) | 2010-04-16 | 2019-07-24 | Momentum Bioscience Limited | Methods for measuring enzyme activity useful in determining cell viability in non-purified samples |
| EP2800822B1 (en) * | 2012-01-05 | 2017-03-29 | Zeus Scientific, Inc. | Improved dna polymerase activity assays and methods enabling detection of viable microbes |
| KR20150009539A (ko) * | 2012-04-12 | 2015-01-26 | 제우스 사이언티픽, 아이엔씨. | 어떤 중합효소 연장 활성의 민감한, 정량적 측정에 유용한 중합효소 활성을 측정하고 생육성 세포의 존재를 결정하는 방법 |
| GB2528647A (en) * | 2014-07-10 | 2016-02-03 | Momentum Bioscience Ltd | Detecting viable microorganisms |
| GB201412316D0 (en) * | 2014-07-10 | 2014-08-27 | Momentum Bioscience Ltd | Detecting the absence of micro-organisms |
| US9879328B2 (en) * | 2014-11-21 | 2018-01-30 | GeneWeave Biosciences, Inc. | Mechanisms of antimicrobial susceptibility |
| US10662484B1 (en) | 2016-03-24 | 2020-05-26 | Bench in a Box, LLC | Two universal duplex primer sets and a combined real-time PCR and high resolution melt analysis assay for the amplification of gram-positive and gram-negative bacteria |
| GB201805479D0 (en) * | 2018-04-03 | 2018-05-16 | Momentum Bioscience Ltd | Microorganism separation and detection |
| GB201914538D0 (en) | 2019-10-08 | 2019-11-20 | Momentum Bioscience Ltd | Microorganism capture from antimicrobial-containing solution |
| US20230112608A1 (en) | 2021-10-13 | 2023-04-13 | Disruptive Oil And Gas Technologies Corp | Nanobubble dispersions generated in electrochemically activated solutions |
| CN114032285B (zh) * | 2021-11-24 | 2022-05-24 | 北京擎科生物科技有限公司 | PolyA聚合酶活性检测方法 |
| WO2025122959A1 (en) * | 2023-12-07 | 2025-06-12 | California Institute Of Technology | Methods for the translated alignment of transcriptomic data at single-cell resolution |
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| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
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| DE4027616A1 (de) | 1990-08-31 | 1992-03-05 | Boehringer Mannheim Gmbh | Verfahren zur bestimmung von polymerase-aktivitaet |
| JPH06509477A (ja) | 1992-05-11 | 1994-10-27 | シュープバッハ・イエルク | 逆転写酵素の検出方法 |
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| US6054278A (en) * | 1997-05-05 | 2000-04-25 | The Perkin-Elmer Corporation | Ribosomal RNA gene polymorphism based microorganism identification |
| ATE434667T1 (de) * | 1999-07-28 | 2009-07-15 | Kaken Pharma Co Ltd | Verfahren zum nachweis pathogener mikroorganismen und antimikrobieller mittel, verfahren zum nachweis der arzneimittelwirkung antimikrobieller mittel und antimikrobielle mittel |
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| GB0508981D0 (en) * | 2005-05-03 | 2005-06-08 | Acolyte Biomedica Ltd | Distinguishing cells in a sample |
| CA2609487A1 (en) * | 2005-05-18 | 2006-11-23 | Iseao Technologies Limited | Improved methods and kits for detecting an enzyme capable of modifying a nucleic acid |
| EP1963528A4 (en) * | 2005-12-20 | 2009-11-25 | Ming-Sheng Lee | APPARATUS, METHODS AND PRODUCTS FOR DETECTION OF GENETIC MUTATION |
| US7947447B2 (en) * | 2007-01-16 | 2011-05-24 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
| WO2009007719A2 (en) * | 2007-07-09 | 2009-01-15 | Iseao Technologies Limited | Detection of micro-organisms based on their nad-dependent dna ligase activity |
| EP3284831B1 (en) | 2010-04-16 | 2019-07-24 | Momentum Bioscience Limited | Methods for measuring enzyme activity useful in determining cell viability in non-purified samples |
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