JP2013537399A - 非精製サンプル中の細胞生存能力を決定することにおいて有用な酵素活性を測定するための方法 - Google Patents

非精製サンプル中の細胞生存能力を決定することにおいて有用な酵素活性を測定するための方法 Download PDF

Info

Publication number
JP2013537399A
JP2013537399A JP2013505162A JP2013505162A JP2013537399A JP 2013537399 A JP2013537399 A JP 2013537399A JP 2013505162 A JP2013505162 A JP 2013505162A JP 2013505162 A JP2013505162 A JP 2013505162A JP 2013537399 A JP2013537399 A JP 2013537399A
Authority
JP
Japan
Prior art keywords
sample
polymerase
nucleic acid
microorganism
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2013505162A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013537399A5 (enExample
Inventor
ショーン・マーク・オハラ
ダニエル・アール・ズウェイツィグ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Daniel R Zweitzig
Zeus Scientific Inc
Original Assignee
Daniel R Zweitzig
Zeus Scientific Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44799329&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP2013537399(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Daniel R Zweitzig, Zeus Scientific Inc filed Critical Daniel R Zweitzig
Publication of JP2013537399A publication Critical patent/JP2013537399A/ja
Publication of JP2013537399A5 publication Critical patent/JP2013537399A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2013505162A 2010-04-16 2011-04-15 非精製サンプル中の細胞生存能力を決定することにおいて有用な酵素活性を測定するための方法 Pending JP2013537399A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US32493910P 2010-04-16 2010-04-16
US32494910P 2010-04-16 2010-04-16
US61/324,949 2010-04-16
US61/324,939 2010-04-16
US32541310P 2010-04-19 2010-04-19
US61/325,413 2010-04-19
PCT/US2011/032600 WO2011130584A2 (en) 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2017086485A Division JP6603956B2 (ja) 2010-04-16 2017-04-25 ポリメラーゼ活性を指標として非精製サンプル中の微生物の存在を検出する方法

Publications (2)

Publication Number Publication Date
JP2013537399A true JP2013537399A (ja) 2013-10-03
JP2013537399A5 JP2013537399A5 (enExample) 2016-03-10

Family

ID=44799329

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2013505162A Pending JP2013537399A (ja) 2010-04-16 2011-04-15 非精製サンプル中の細胞生存能力を決定することにおいて有用な酵素活性を測定するための方法
JP2017086485A Active JP6603956B2 (ja) 2010-04-16 2017-04-25 ポリメラーゼ活性を指標として非精製サンプル中の微生物の存在を検出する方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2017086485A Active JP6603956B2 (ja) 2010-04-16 2017-04-25 ポリメラーゼ活性を指標として非精製サンプル中の微生物の存在を検出する方法

Country Status (16)

Country Link
US (3) US20130196318A1 (enExample)
EP (3) EP3284831B1 (enExample)
JP (2) JP2013537399A (enExample)
KR (2) KR20130094714A (enExample)
CN (1) CN103328652B (enExample)
AP (1) AP2012006530A0 (enExample)
AU (2) AU2011239587B2 (enExample)
CA (1) CA2796676C (enExample)
EA (1) EA201291001A1 (enExample)
ES (3) ES2906061T3 (enExample)
MY (1) MY161567A (enExample)
NZ (2) NZ701866A (enExample)
PH (2) PH12012502065A1 (enExample)
SG (2) SG10201502900PA (enExample)
WO (1) WO2011130584A2 (enExample)
ZA (1) ZA201208522B (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015503925A (ja) * 2012-01-05 2015-02-05 ゼウス・サイエンティフィック・インコーポレイテッドＺｅｕｓ Ｓｃｉｅｎｔｉｆｉｃ， Ｉｎｃ． 改良したｄｎａポリメラーゼ活性アッセイおよび生菌の検出を可能にする方法
JP2017525386A (ja) * 2014-07-10 2017-09-07 モメンタム・バイオサイエンス・リミテッドＭｏｍｅｎｔｕｍ Ｂｉｏｓｃｉｅｎｃｅ Ｌｉｍｉｔｅｄ 微生物の非存在の検出の方法およびキット

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3284831B1 (en) 2010-04-16 2019-07-24 Momentum Bioscience Limited Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
KR20150009539A (ko) * 2012-04-12 2015-01-26 제우스 사이언티픽, 아이엔씨. 어떤 중합효소 연장 활성의 민감한, 정량적 측정에 유용한 중합효소 활성을 측정하고 생육성 세포의 존재를 결정하는 방법
GB2528647A (en) * 2014-07-10 2016-02-03 Momentum Bioscience Ltd Detecting viable microorganisms
US9879328B2 (en) * 2014-11-21 2018-01-30 GeneWeave Biosciences, Inc. Mechanisms of antimicrobial susceptibility
US10662484B1 (en) 2016-03-24 2020-05-26 Bench in a Box, LLC Two universal duplex primer sets and a combined real-time PCR and high resolution melt analysis assay for the amplification of gram-positive and gram-negative bacteria
GB201805479D0 (en) * 2018-04-03 2018-05-16 Momentum Bioscience Ltd Microorganism separation and detection
GB201914538D0 (en) 2019-10-08 2019-11-20 Momentum Bioscience Ltd Microorganism capture from antimicrobial-containing solution
US20230112608A1 (en) 2021-10-13 2023-04-13 Disruptive Oil And Gas Technologies Corp Nanobubble dispersions generated in electrochemically activated solutions
CN114032285B (zh) * 2021-11-24 2022-05-24 北京擎科生物科技有限公司 PolyA聚合酶活性检测方法
WO2025122959A1 (en) * 2023-12-07 2025-06-12 California Institute Of Technology Methods for the translated alignment of transcriptomic data at single-cell resolution
GB202405229D0 (en) 2024-04-12 2024-05-29 Momentum Bioscience Ltd Microorganism enumeration

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6054697A (ja) * 1983-09-05 1985-03-29 イザド−ラ　ブロドスキイ 診断方法および装置
JPH04501953A (ja) * 1988-11-30 1992-04-09 サビデイ・テツク・アー・ベー ポリメラーゼ活性測定法
JPH05505942A (ja) * 1990-08-31 1993-09-02 ベーリンガー・マンハイム・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング ポリメラーゼ活性測定方法

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS501953B1 (enExample) 1968-08-05 1975-01-22
JPS505942B1 (enExample) 1970-08-23 1975-03-08
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
ATE143696T1 (de) * 1989-02-28 1996-10-15 Canon Kk Partiell doppelsträngiges oligonukleotid und verfahren zu seiner bildung
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
JPH06509477A (ja) 1992-05-11 1994-10-27 シュープバッハ・イエルク 逆転写酵素の検出方法
US5629154A (en) † 1993-11-12 1997-05-13 Geron Corporation Telomerase activity assays
US5489508A (en) † 1992-05-13 1996-02-06 University Of Texas System Board Of Regents Therapy and diagnosis of conditions related to telomere length and/or telomerase activity
JPH0622798A (ja) * 1992-07-07 1994-02-01 Hitachi Ltd 塩基配列決定法
US5719028A (en) * 1992-12-07 1998-02-17 Third Wave Technologies Inc. Cleavase fragment length polymorphism
US5863726A (en) † 1993-11-12 1999-01-26 Geron Corporation Telomerase activity assays
JP3856467B2 (ja) 1994-07-13 2006-12-13 イギリス国 微生物学的試験方法および試薬
US6054278A (en) * 1997-05-05 2000-04-25 The Perkin-Elmer Corporation Ribosomal RNA gene polymorphism based microorganism identification
ATE434667T1 (de) * 1999-07-28 2009-07-15 Kaken Pharma Co Ltd Verfahren zum nachweis pathogener mikroorganismen und antimikrobieller mittel, verfahren zum nachweis der arzneimittelwirkung antimikrobieller mittel und antimikrobielle mittel
GB9923846D0 (en) 1999-10-09 1999-12-08 Quip Technology Limited Reverse transcriptase assay
EP1529117A2 (en) * 2002-08-08 2005-05-11 University of Lausanne Method for the rapid assessment of the presence and viability of bacterial cells and use thereof
US20050136439A1 (en) * 2003-09-12 2005-06-23 North Carolina State University Novel methods of inorganic compound discovery and synthesis
GB0508981D0 (en) * 2005-05-03 2005-06-08 Acolyte Biomedica Ltd Distinguishing cells in a sample
CA2609487A1 (en) * 2005-05-18 2006-11-23 Iseao Technologies Limited Improved methods and kits for detecting an enzyme capable of modifying a nucleic acid
EP1963528A4 (en) * 2005-12-20 2009-11-25 Ming-Sheng Lee APPARATUS, METHODS AND PRODUCTS FOR DETECTION OF GENETIC MUTATION
US7947447B2 (en) * 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
WO2009007719A2 (en) * 2007-07-09 2009-01-15 Iseao Technologies Limited Detection of micro-organisms based on their nad-dependent dna ligase activity
EP3284831B1 (en) 2010-04-16 2019-07-24 Momentum Bioscience Limited Methods for measuring enzyme activity useful in determining cell viability in non-purified samples

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6054697A (ja) * 1983-09-05 1985-03-29 イザド−ラ　ブロドスキイ 診断方法および装置
JPH04501953A (ja) * 1988-11-30 1992-04-09 サビデイ・テツク・アー・ベー ポリメラーゼ活性測定法
JPH05505942A (ja) * 1990-08-31 1993-09-02 ベーリンガー・マンハイム・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング ポリメラーゼ活性測定方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015503925A (ja) * 2012-01-05 2015-02-05 ゼウス・サイエンティフィック・インコーポレイテッドＺｅｕｓ Ｓｃｉｅｎｔｉｆｉｃ， Ｉｎｃ． 改良したｄｎａポリメラーゼ活性アッセイおよび生菌の検出を可能にする方法
JP2017525386A (ja) * 2014-07-10 2017-09-07 モメンタム・バイオサイエンス・リミテッドＭｏｍｅｎｔｕｍ Ｂｉｏｓｃｉｅｎｃｅ Ｌｉｍｉｔｅｄ 微生物の非存在の検出の方法およびキット

Also Published As

Publication number Publication date
KR102068136B1 (ko) 2020-01-20
EP3284831B1 (en) 2019-07-24
EP2558600A2 (en) 2013-02-20
EP2558600A4 (en) 2014-04-30
US10870894B2 (en) 2020-12-22
EP3617320B1 (en) 2022-01-26
MY161567A (en) 2017-04-28
WO2011130584A3 (en) 2013-07-11
SG10201502900PA (en) 2015-06-29
EP2558600B1 (en) 2017-10-04
AU2011239587B2 (en) 2016-03-10
AU2011239587A1 (en) 2012-11-08
EP3617320A1 (en) 2020-03-04
US20130196318A1 (en) 2013-08-01
US20170321287A1 (en) 2017-11-09
EA201291001A1 (ru) 2013-09-30
AP2012006530A0 (en) 2012-10-31
CN103328652A (zh) 2013-09-25
ZA201208522B (en) 2014-08-27
US20180230551A1 (en) 2018-08-16
ES2644258T3 (es) 2017-11-28
ES2644258T5 (es) 2021-02-02
NZ720529A (en) 2017-01-27
CN103328652B (zh) 2016-12-28
JP2017148067A (ja) 2017-08-31
ES2906061T3 (es) 2022-04-13
CA2796676A1 (en) 2011-10-20
JP6603956B2 (ja) 2019-11-13
PH12012502065A1 (en) 2019-07-10
PH12016500300A1 (en) 2019-02-04
KR20180086526A (ko) 2018-07-31
KR20130094714A (ko) 2013-08-26
SG184563A1 (en) 2012-11-29
CA2796676C (en) 2020-07-14
EP3284831A1 (en) 2018-02-21
WO2011130584A2 (en) 2011-10-20
NZ701866A (en) 2016-07-29
EP2558600B2 (en) 2020-06-17
US10876173B2 (en) 2020-12-29
AU2016203878A1 (en) 2016-06-30
AP3635A (enExample) 2016-03-09
ES2739806T3 (es) 2020-02-04

Similar Documents

Publication Publication Date Title
JP6603956B2 (ja) ポリメラーゼ活性を指標として非精製サンプル中の微生物の存在を検出する方法
JP2013537399A5 (enExample)
EP2179052B1 (en) Detection of micro-organisms based on their nad-dependent dna ligase activity
US20150093749A1 (en) Real-time pcr detection of streptococcus pyogenes
JP2016192967A (ja) 血液試料の微生物特異的フィルタ・インサイチュ分析の方法
JP2014502510A5 (enExample)
EP2419524B1 (en) Detection of bacteria and fungi
US20150057172A1 (en) Real-time pcr detection of mycobacterium tuberculosis complex
WO2025215382A2 (en) Microorganism enumeration
Class et al. Patent application title: Methods for Detection of Micro-Organisms Inventors: Christopher John Stanley (Cambridge, GB) Stuart Wilson (London, GB) Assignees: MICROSEN MEDTECH LIMITED

Legal Events

Date Code Title Description
RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20140120

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20140414

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140530

RD02 Notification of acceptance of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7422

Effective date: 20140530

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150602

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20150813

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20151001

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20151030

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20151125

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20160307

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20160607

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160906

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170126

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20170814