ES2739806T3 - Métodos para medir actividad enzimática, útiles para determinar la viabilidad celular en muestras no purificadas - Google Patents

Métodos para medir actividad enzimática, útiles para determinar la viabilidad celular en muestras no purificadas Download PDF

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Publication number
ES2739806T3
ES2739806T3 ES17194583T ES17194583T ES2739806T3 ES 2739806 T3 ES2739806 T3 ES 2739806T3 ES 17194583 T ES17194583 T ES 17194583T ES 17194583 T ES17194583 T ES 17194583T ES 2739806 T3 ES2739806 T3 ES 2739806T3
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Prior art keywords
sample
polymerase
microorganism
methods
dna
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ES17194583T
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English (en)
Spanish (es)
Inventor
Shawn Mark O'hara
Daniel R Zweitzig
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Momentum Bioscience Ltd
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Momentum Bioscience Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES17194583T 2010-04-16 2011-04-15 Métodos para medir actividad enzimática, útiles para determinar la viabilidad celular en muestras no purificadas Active ES2739806T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32494910P 2010-04-16 2010-04-16
US32493910P 2010-04-16 2010-04-16
US32541310P 2010-04-19 2010-04-19

Publications (1)

Publication Number Publication Date
ES2739806T3 true ES2739806T3 (es) 2020-02-04

Family

ID=44799329

Family Applications (3)

Application Number Title Priority Date Filing Date
ES17194583T Active ES2739806T3 (es) 2010-04-16 2011-04-15 Métodos para medir actividad enzimática, útiles para determinar la viabilidad celular en muestras no purificadas
ES11769633T Active ES2644258T5 (es) 2010-04-16 2011-04-15 Métodos de medición de la actividad enzimática útiles para determinar la viabilidad celular en muestras no purificadas
ES19187658T Active ES2906061T3 (es) 2010-04-16 2011-04-15 Métodos para medir actividad enzimática, útiles para determinar la viabilidad celular en muestras no purificadas

Family Applications After (2)

Application Number Title Priority Date Filing Date
ES11769633T Active ES2644258T5 (es) 2010-04-16 2011-04-15 Métodos de medición de la actividad enzimática útiles para determinar la viabilidad celular en muestras no purificadas
ES19187658T Active ES2906061T3 (es) 2010-04-16 2011-04-15 Métodos para medir actividad enzimática, útiles para determinar la viabilidad celular en muestras no purificadas

Country Status (16)

Country Link
US (3) US20130196318A1 (enExample)
EP (3) EP3284831B1 (enExample)
JP (2) JP2013537399A (enExample)
KR (2) KR20130094714A (enExample)
CN (1) CN103328652B (enExample)
AP (1) AP2012006530A0 (enExample)
AU (2) AU2011239587B2 (enExample)
CA (1) CA2796676C (enExample)
EA (1) EA201291001A1 (enExample)
ES (3) ES2739806T3 (enExample)
MY (1) MY161567A (enExample)
NZ (2) NZ720529A (enExample)
PH (2) PH12012502065A1 (enExample)
SG (2) SG184563A1 (enExample)
WO (1) WO2011130584A2 (enExample)
ZA (1) ZA201208522B (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
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EP3284831B1 (en) 2010-04-16 2019-07-24 Momentum Bioscience Limited Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
US9708651B2 (en) 2012-01-05 2017-07-18 Zeus Scientific, Inc. DNA polymerase activity assays and methods enabling determination of viable microbes
IN2014DN09421A (enExample) * 2012-04-12 2015-07-17 Zeus Scientific Inc
GB201412316D0 (en) * 2014-07-10 2014-08-27 Momentum Bioscience Ltd Detecting the absence of micro-organisms
GB2528647A (en) * 2014-07-10 2016-02-03 Momentum Bioscience Ltd Detecting viable microorganisms
US9879328B2 (en) * 2014-11-21 2018-01-30 GeneWeave Biosciences, Inc. Mechanisms of antimicrobial susceptibility
US10662484B1 (en) 2016-03-24 2020-05-26 Bench in a Box, LLC Two universal duplex primer sets and a combined real-time PCR and high resolution melt analysis assay for the amplification of gram-positive and gram-negative bacteria
GB201805479D0 (en) 2018-04-03 2018-05-16 Momentum Bioscience Ltd Microorganism separation and detection
GB201914538D0 (en) 2019-10-08 2019-11-20 Momentum Bioscience Ltd Microorganism capture from antimicrobial-containing solution
US20230112608A1 (en) 2021-10-13 2023-04-13 Disruptive Oil And Gas Technologies Corp Nanobubble dispersions generated in electrochemically activated solutions
CN114032285B (zh) * 2021-11-24 2022-05-24 北京擎科生物科技有限公司 PolyA聚合酶活性检测方法
WO2025122959A1 (en) * 2023-12-07 2025-06-12 California Institute Of Technology Methods for the translated alignment of transcriptomic data at single-cell resolution
GB202405229D0 (en) 2024-04-12 2024-05-29 Momentum Bioscience Ltd Microorganism enumeration

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JPS505942B1 (enExample) 1970-08-23 1975-03-08
JPS6054697A (ja) * 1983-09-05 1985-03-29 イザド−ラ ブロドスキイ 診断方法および装置
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
SE462497B (sv) * 1988-11-30 1990-07-02 Clas Fredrik Runesson Kaelland Saett foer att aktivitetsbestaemma polymeraser
ATE143696T1 (de) * 1989-02-28 1996-10-15 Canon Kk Partiell doppelsträngiges oligonukleotid und verfahren zu seiner bildung
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
DE4027616A1 (de) * 1990-08-31 1992-03-05 Boehringer Mannheim Gmbh Verfahren zur bestimmung von polymerase-aktivitaet
AU4258693A (en) 1992-05-11 1993-12-13 Jurg Boni Process for detecting reverse transcriptase
US5629154A (en) 1993-11-12 1997-05-13 Geron Corporation Telomerase activity assays
US5489508A (en) 1992-05-13 1996-02-06 University Of Texas System Board Of Regents Therapy and diagnosis of conditions related to telomere length and/or telomerase activity
JPH0622798A (ja) * 1992-07-07 1994-02-01 Hitachi Ltd 塩基配列決定法
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US5863726A (en) 1993-11-12 1999-01-26 Geron Corporation Telomerase activity assays
BR9408598A (pt) 1994-07-13 1997-11-18 Sec Of State For Defence In He Processo para determinar a presença e/ou o teor de microorganismos e/ou seus materiais intracelulares presentes em uma amostra kit de teste e reagente
US6054278A (en) * 1997-05-05 2000-04-25 The Perkin-Elmer Corporation Ribosomal RNA gene polymorphism based microorganism identification
JP4414623B2 (ja) * 1999-07-28 2010-02-10 科研製薬株式会社 病原微生物および抗微生物剤の検出法、抗微生物剤の薬効評価法ならびに抗微生物剤
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EP1529117A2 (en) * 2002-08-08 2005-05-11 University of Lausanne Method for the rapid assessment of the presence and viability of bacterial cells and use thereof
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EP3284831B1 (en) 2010-04-16 2019-07-24 Momentum Bioscience Limited Methods for measuring enzyme activity useful in determining cell viability in non-purified samples

Also Published As

Publication number Publication date
CN103328652A (zh) 2013-09-25
AU2011239587B2 (en) 2016-03-10
CA2796676C (en) 2020-07-14
EP2558600A4 (en) 2014-04-30
WO2011130584A2 (en) 2011-10-20
EP3617320B1 (en) 2022-01-26
JP6603956B2 (ja) 2019-11-13
ES2644258T3 (es) 2017-11-28
ES2644258T5 (es) 2021-02-02
EA201291001A1 (ru) 2013-09-30
US10876173B2 (en) 2020-12-29
JP2017148067A (ja) 2017-08-31
EP3284831B1 (en) 2019-07-24
AU2016203878A1 (en) 2016-06-30
JP2013537399A (ja) 2013-10-03
SG184563A1 (en) 2012-11-29
WO2011130584A3 (en) 2013-07-11
EP2558600B2 (en) 2020-06-17
AP2012006530A0 (en) 2012-10-31
US20180230551A1 (en) 2018-08-16
KR20180086526A (ko) 2018-07-31
NZ701866A (en) 2016-07-29
EP2558600B1 (en) 2017-10-04
MY161567A (en) 2017-04-28
AP3635A (enExample) 2016-03-09
EP3284831A1 (en) 2018-02-21
CA2796676A1 (en) 2011-10-20
SG10201502900PA (en) 2015-06-29
KR20130094714A (ko) 2013-08-26
NZ720529A (en) 2017-01-27
PH12012502065A1 (en) 2019-07-10
AU2011239587A1 (en) 2012-11-08
US20130196318A1 (en) 2013-08-01
KR102068136B1 (ko) 2020-01-20
PH12016500300A1 (en) 2019-02-04
US20170321287A1 (en) 2017-11-09
ZA201208522B (en) 2014-08-27
CN103328652B (zh) 2016-12-28
EP3617320A1 (en) 2020-03-04
ES2906061T3 (es) 2022-04-13
EP2558600A2 (en) 2013-02-20
US10870894B2 (en) 2020-12-22

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