EA201291001A1 - Способы измерения ферментативной активности, полезные при определении жизнеспособности клеток в неочищенных образцах - Google Patents

Способы измерения ферментативной активности, полезные при определении жизнеспособности клеток в неочищенных образцах

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Publication number
EA201291001A1
EA201291001A1 EA201291001A EA201291001A EA201291001A1 EA 201291001 A1 EA201291001 A1 EA 201291001A1 EA 201291001 A EA201291001 A EA 201291001A EA 201291001 A EA201291001 A EA 201291001A EA 201291001 A1 EA201291001 A1 EA 201291001A1
Authority
EA
Eurasian Patent Office
Prior art keywords
methods
activity
useful
determining
enzymative
Prior art date
Application number
EA201291001A
Other languages
English (en)
Inventor
Шон Марк О'Хара
Дэниел Р. Цвайтциг
Original Assignee
Зиус Сайентифик, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44799329&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA201291001(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Зиус Сайентифик, Инк. filed Critical Зиус Сайентифик, Инк.
Publication of EA201291001A1 publication Critical patent/EA201291001A1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Данное изобретение относится в общем к области обнаружения микроорганизмов, в частности к обнаружению бактерий, к способам измерения ферментативной активности, такой как активность ДНК-полимеразы, и, в частности, относится к таким способам, осуществляемым на микробных неочищенных лизатах, полезных для определения микробных ферментативных активностей, которые могут быть связаны с генераторами амплификационного сигнала, такими как способы полимеразной цепной реакции (ПЦР) в реальном времени, обеспечивая таким образом возможность определения микробных патогенов в образцах, таких как неочищенная кровь и другие жидкости организма. Данное изобретение также относится к реагентам для использования в таких способах, и к тестирующим наборам, включающим такие реагенты для осуществления способов.
EA201291001A 2010-04-16 2011-04-15 Способы измерения ферментативной активности, полезные при определении жизнеспособности клеток в неочищенных образцах EA201291001A1 (ru)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US32494910P 2010-04-16 2010-04-16
US32493910P 2010-04-16 2010-04-16
US32541310P 2010-04-19 2010-04-19
PCT/US2011/032600 WO2011130584A2 (en) 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples

Publications (1)

Publication Number Publication Date
EA201291001A1 true EA201291001A1 (ru) 2013-09-30

Family

ID=44799329

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201291001A EA201291001A1 (ru) 2010-04-16 2011-04-15 Способы измерения ферментативной активности, полезные при определении жизнеспособности клеток в неочищенных образцах

Country Status (16)

Country Link
US (3) US20130196318A1 (ru)
EP (3) EP2558600B2 (ru)
JP (2) JP2013537399A (ru)
KR (2) KR102068136B1 (ru)
CN (1) CN103328652B (ru)
AP (1) AP2012006530A0 (ru)
AU (2) AU2011239587B2 (ru)
CA (1) CA2796676C (ru)
EA (1) EA201291001A1 (ru)
ES (3) ES2739806T3 (ru)
MY (1) MY161567A (ru)
NZ (2) NZ720529A (ru)
PH (1) PH12016500300A1 (ru)
SG (2) SG184563A1 (ru)
WO (1) WO2011130584A2 (ru)
ZA (1) ZA201208522B (ru)

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SG184563A1 (en) 2010-04-16 2012-11-29 Zeus Scientific Inc Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
EP2800822B1 (en) * 2012-01-05 2017-03-29 Zeus Scientific, Inc. Improved dna polymerase activity assays and methods enabling detection of viable microbes
CA2908941A1 (en) * 2012-04-12 2013-10-17 Zeus Scientific, Inc. Methods for measuring polymerase activity useful for sensitive, quantitative measurements of any polymerase extension activity and for determining the presence of viable cells
GB201412316D0 (en) 2014-07-10 2014-08-27 Momentum Bioscience Ltd Detecting the absence of micro-organisms
GB2528647A (en) * 2014-07-10 2016-02-03 Momentum Bioscience Ltd Detecting viable microorganisms
US9879328B2 (en) * 2014-11-21 2018-01-30 GeneWeave Biosciences, Inc. Mechanisms of antimicrobial susceptibility
US10662484B1 (en) 2016-03-24 2020-05-26 Bench in a Box, LLC Two universal duplex primer sets and a combined real-time PCR and high resolution melt analysis assay for the amplification of gram-positive and gram-negative bacteria
GB201805479D0 (en) * 2018-04-03 2018-05-16 Momentum Bioscience Ltd Microorganism separation and detection
GB201914538D0 (en) 2019-10-08 2019-11-20 Momentum Bioscience Ltd Microorganism capture from antimicrobial-containing solution
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Also Published As

Publication number Publication date
EP2558600B1 (en) 2017-10-04
NZ701866A (en) 2016-07-29
US10876173B2 (en) 2020-12-29
EP2558600A4 (en) 2014-04-30
MY161567A (en) 2017-04-28
EP2558600A2 (en) 2013-02-20
ES2906061T3 (es) 2022-04-13
EP3617320A1 (en) 2020-03-04
KR20180086526A (ko) 2018-07-31
ES2739806T3 (es) 2020-02-04
AP3635A (ru) 2016-03-09
EP3617320B1 (en) 2022-01-26
US20180230551A1 (en) 2018-08-16
AU2011239587B2 (en) 2016-03-10
AU2016203878A1 (en) 2016-06-30
CN103328652B (zh) 2016-12-28
SG10201502900PA (en) 2015-06-29
SG184563A1 (en) 2012-11-29
US20170321287A1 (en) 2017-11-09
JP6603956B2 (ja) 2019-11-13
JP2017148067A (ja) 2017-08-31
AP2012006530A0 (en) 2012-10-31
EP3284831B1 (en) 2019-07-24
JP2013537399A (ja) 2013-10-03
WO2011130584A3 (en) 2013-07-11
US20130196318A1 (en) 2013-08-01
CA2796676A1 (en) 2011-10-20
WO2011130584A2 (en) 2011-10-20
PH12016500300A1 (en) 2019-02-04
CN103328652A (zh) 2013-09-25
NZ720529A (en) 2017-01-27
AU2011239587A1 (en) 2012-11-08
ES2644258T3 (es) 2017-11-28
ES2644258T5 (es) 2021-02-02
ZA201208522B (en) 2014-08-27
CA2796676C (en) 2020-07-14
EP3284831A1 (en) 2018-02-21
KR102068136B1 (ko) 2020-01-20
EP2558600B2 (en) 2020-06-17
KR20130094714A (ko) 2013-08-26
US10870894B2 (en) 2020-12-22

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