WO2009011277A1 - 抗酸菌属細菌の検出および同定用オリゴヌクレオチド、およびその用途 - Google Patents

抗酸菌属細菌の検出および同定用オリゴヌクレオチド、およびその用途 Download PDF

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Publication number
WO2009011277A1
WO2009011277A1 PCT/JP2008/062479 JP2008062479W WO2009011277A1 WO 2009011277 A1 WO2009011277 A1 WO 2009011277A1 JP 2008062479 W JP2008062479 W JP 2008062479W WO 2009011277 A1 WO2009011277 A1 WO 2009011277A1
Authority
WO
WIPO (PCT)
Prior art keywords
acid
detection
fast bacterium
identification
oligonucleotide
Prior art date
Application number
PCT/JP2008/062479
Other languages
English (en)
French (fr)
Inventor
Masahiro Kusumoto
Original Assignee
Toyo Boseki Kabushiki Kaisha
Shiuchi, Keiichi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyo Boseki Kabushiki Kaisha, Shiuchi, Keiichi filed Critical Toyo Boseki Kabushiki Kaisha
Publication of WO2009011277A1 publication Critical patent/WO2009011277A1/ja

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

【課題】臨床診断上重要な抗酸菌属細菌について迅速、確実かつ簡便に検出または菌種同定する方法を提供する。 【解決手段】抗酸菌属細菌のｄｎａＪ１遺伝子において菌種特異性が極めて高い領域を標的核酸とするプローブを用いた融解曲線解析を行う。  
PCT/JP2008/062479 2007-07-13 2008-07-10 抗酸菌属細菌の検出および同定用オリゴヌクレオチド、およびその用途 WO2009011277A1 (ja)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
JP2007-184729 2007-07-13
JP2007184729 2007-07-13
JP2007184731 2007-07-13
JP2007-184730 2007-07-13
JP2007-184731 2007-07-13
JP2007184732 2007-07-13
JP2007-184732 2007-07-13
JP2007184730 2007-07-13

Publications (1)

Publication Number Publication Date
WO2009011277A1 true WO2009011277A1 (ja) 2009-01-22

Family

ID=40259613

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/062479 WO2009011277A1 (ja) 2007-07-13 2008-07-10 抗酸菌属細菌の検出および同定用オリゴヌクレオチド、およびその用途

Country Status (1)

Country Link
WO (1) WO2009011277A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112501324A (zh) * 2020-11-26 2021-03-16 广州迪澳生物科技有限公司 一种基于环介导等温扩增检测结核分枝杆菌复合群及非结核分枝杆菌复合群的引物、试剂盒

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0436199A (ja) * 1990-05-31 1992-02-06 Iatron Lab Inc ミコバクテリア属微生物の検出方法
JPH09511658A (ja) * 1995-08-28 1997-11-25 ベクトン・ディッキンソン・アンド・カンパニー 鳥型結核菌複合種の増幅および検出
JPH10323189A (ja) * 1997-05-23 1998-12-08 Toyobo Co Ltd 抗酸菌属細菌の検出または菌種同定用オリゴヌクレオチドおよびその用途
JP2001286300A (ja) * 1999-04-20 2001-10-16 Japan Bioindustry Association 核酸の測定方法、それに用いる核酸プローブ及びその方法によって得られるデータを解析する方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0436199A (ja) * 1990-05-31 1992-02-06 Iatron Lab Inc ミコバクテリア属微生物の検出方法
JPH09511658A (ja) * 1995-08-28 1997-11-25 ベクトン・ディッキンソン・アンド・カンパニー 鳥型結核菌複合種の増幅および検出
JPH10323189A (ja) * 1997-05-23 1998-12-08 Toyobo Co Ltd 抗酸菌属細菌の検出または菌種同定用オリゴヌクレオチドおよびその用途
JP2001286300A (ja) * 1999-04-20 2001-10-16 Japan Bioindustry Association 核酸の測定方法、それに用いる核酸プローブ及びその方法によって得られるデータを解析する方法

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
COLE, S. T. ET AL.: "Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.", NATURE, vol. 393, no. 6685, 1998, pages 537 - 544, XP003018482 *
DATABASE NCBI [o] 19 October 2006 (2006-10-19), "M. tuberculosis dnaJ gene", Database accession no. X06422 *
MAKIKO NODA ET AL.: "Kosankin no Taso Idenshi Kaiseki kara Mita Kinshukan no Idenshi Hairetsu no Takei", JAPANESE JOURNAL OF BACTERIOLOGY., vol. 62, no. 1, February 2007 (2007-02-01), pages 53 *
MAKIKO NODA ET AL.: "Mycobacterium-zoku Kin no dnaJ Hairetsu ni Motozuku Keito Kaiseki", JAPANESE JOURNAL OF BACTERIOLOGY., vol. 61, no. 1, 2006, pages 136 *
STEWART, GRAHAM R. ET AL.: "Analysis of the function of mycobacterial DnaJ proteins by overexpression and microarray profiling.", TUBERCULOSIS, vol. 84, no. 3-4, 2004, pages 180 - 187, XP055354869 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112501324A (zh) * 2020-11-26 2021-03-16 广州迪澳生物科技有限公司 一种基于环介导等温扩增检测结核分枝杆菌复合群及非结核分枝杆菌复合群的引物、试剂盒

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