ES2732735T3 - Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas - Google Patents

Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas Download PDF

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ES2732735T3
ES2732735T3 ES16163925T ES16163925T ES2732735T3 ES 2732735 T3 ES2732735 T3 ES 2732735T3 ES 16163925 T ES16163925 T ES 16163925T ES 16163925 T ES16163925 T ES 16163925T ES 2732735 T3 ES2732735 T3 ES 2732735T3
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meganuclease
sequence
laglidadg
seq
subunit
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James Smith
Derek Jantz
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Precision Biosciences Inc
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Precision Biosciences Inc
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
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    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/04—Phosphoric diester hydrolases (3.1.4)

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  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
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  • Communicable Diseases (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
ES16163925T 2007-10-31 2008-10-31 Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas Active ES2732735T3 (es)

Applications Claiming Priority (1)

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US124707P 2007-10-31 2007-10-31

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ES16163925T Active ES2732735T3 (es) 2007-10-31 2008-10-31 Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas
ES13165733.0T Active ES2575412T3 (es) 2007-10-31 2008-10-31 Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas
ES08845549T Active ES2422291T3 (es) 2007-10-31 2008-10-31 Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas

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ES13165733.0T Active ES2575412T3 (es) 2007-10-31 2008-10-31 Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas
ES08845549T Active ES2422291T3 (es) 2007-10-31 2008-10-31 Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas

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US (10) US8445251B2 (https=)
EP (4) EP2215223B1 (https=)
JP (7) JP5761996B2 (https=)
AU (1) AU2008318430A1 (https=)
CA (1) CA2704383A1 (https=)
DK (3) DK2215223T3 (https=)
ES (3) ES2732735T3 (https=)
WO (1) WO2009059195A2 (https=)

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