ES2676062T3 - Procedimientos para el enriquecimiento de ácido nucleico mutado de una mezcla - Google Patents
Procedimientos para el enriquecimiento de ácido nucleico mutado de una mezcla Download PDFInfo
- Publication number
- ES2676062T3 ES2676062T3 ES14805538.7T ES14805538T ES2676062T3 ES 2676062 T3 ES2676062 T3 ES 2676062T3 ES 14805538 T ES14805538 T ES 14805538T ES 2676062 T3 ES2676062 T3 ES 2676062T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acid
- target nucleic
- variant
- oligonucleotide
- double helix
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361909587P | 2013-11-27 | 2013-11-27 | |
| US201361909587P | 2013-11-27 | ||
| PCT/EP2014/075460 WO2015078831A1 (en) | 2013-11-27 | 2014-11-25 | Methods for the enrichment of mutated nucleic acid from a mixture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2676062T3 true ES2676062T3 (es) | 2018-07-16 |
Family
ID=52000821
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES14805538.7T Active ES2676062T3 (es) | 2013-11-27 | 2014-11-25 | Procedimientos para el enriquecimiento de ácido nucleico mutado de una mezcla |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9873908B2 (enExample) |
| EP (1) | EP3074529B1 (enExample) |
| JP (1) | JP6553598B2 (enExample) |
| CN (1) | CN105745336B (enExample) |
| CA (1) | CA2926693C (enExample) |
| ES (1) | ES2676062T3 (enExample) |
| WO (1) | WO2015078831A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2665500T3 (es) | 2010-03-08 | 2018-04-26 | Dana-Farber Cancer Institute, Inc. | Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia |
| EP2691541B1 (en) | 2011-03-31 | 2017-10-18 | Dana-Farber Cancer Institute, Inc. | Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences |
| PL3246416T3 (pl) | 2011-04-15 | 2024-09-30 | The Johns Hopkins University | Bezpieczny system sekwencjonowania |
| CN109457030B (zh) | 2012-10-29 | 2022-02-18 | 约翰·霍普金斯大学 | 卵巢和子宫内膜癌的帕帕尼科拉乌测试 |
| ES2917175T3 (es) | 2015-06-24 | 2022-07-07 | Dana Farber Cancer Inst Inc | Degradación selectiva de ADN de tipo salvaje y enriquecimiento de alelos mutantes usando nucleasa |
| US11286531B2 (en) | 2015-08-11 | 2022-03-29 | The Johns Hopkins University | Assaying ovarian cyst fluid |
| US11371090B2 (en) | 2016-12-12 | 2022-06-28 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection |
| EP3665308A1 (en) | 2017-08-07 | 2020-06-17 | The Johns Hopkins University | Methods and materials for assessing and treating cancer |
| US12571023B2 (en) * | 2018-02-26 | 2026-03-10 | Spark Molecular Diagnostics, Inc. | Composition and methods for affinity directed enrichment of rare species |
| AU2019307835B2 (en) | 2018-07-19 | 2021-08-19 | Biofidelity Ltd | Improved polynucleotide sequence detection method |
| GB201919186D0 (en) | 2019-12-23 | 2020-02-05 | Biofidelity Ltd | Simplified polynucleotide sequence detection method |
| BR112022015909A2 (pt) | 2020-02-14 | 2022-10-04 | Univ Johns Hopkins | Métodos e materiais para avaliação de ácidos nucleicos |
| JP7760607B2 (ja) * | 2021-04-15 | 2025-10-27 | バイオフィデリティー | 核酸の濃縮及び検出 |
| CN113774113B (zh) * | 2021-09-17 | 2023-07-04 | 广州医科大学附属第一医院（广州呼吸中心） | 一种富集突变基因序列的方法和应用 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6120992A (en) * | 1993-11-04 | 2000-09-19 | Valigene Corporation | Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human |
| EP0929694A4 (en) | 1996-03-15 | 2002-05-02 | Penn State Res Found | DETECTION OF TUMOR-RELATED EXTRACELLULAR NUCLEIC ACID USING PLASMA OR BLOOD SERUM USING NUCLEIC ACID AMPLIFICATION TESTS |
| US6630301B1 (en) | 1997-03-14 | 2003-10-07 | The Penn State Research Foundation | Detection of extracellular tumor-associated nucleic acid in blood plasma or serum |
| US6031098A (en) | 1997-08-11 | 2000-02-29 | California Institute Of Technology | Detection and treatment of duplex polynucleotide damage |
| SE9902741D0 (sv) | 1999-07-19 | 1999-07-19 | Boerries Kemper | Method for the enrichment of heteroduplexes and its use in mutation detection |
| US20030082537A1 (en) * | 2000-05-23 | 2003-05-01 | Stanton Vincent P. | Methods for genetic analysis of DNA to detect sequence variances |
| US20020132244A1 (en) | 2000-09-27 | 2002-09-19 | Xiao-Cheng Li-Sucholeiki | Methods for detecting rare polymorphic variants in genomic DNA sequences |
| WO2002057492A1 (en) * | 2000-12-08 | 2002-07-25 | California Institute Of Technology | Methods and compositions for detecting polynucleotide duplex damage |
| WO2002086196A1 (en) * | 2001-04-19 | 2002-10-31 | Rd Chemical Company | Copper acid baths, system and method for electroplating high aspect ratio substrates |
| WO2002086169A1 (en) | 2001-04-23 | 2002-10-31 | Dana-Farber Cancer Institute, Inc. | Methods for rapid screening of polymorphisms, mutations and methylation |
| GB2382137A (en) | 2001-11-20 | 2003-05-21 | Mats Gullberg | Nucleic acid enrichment |
| WO2005085473A2 (en) * | 2004-03-01 | 2005-09-15 | University Of Chicago | Polymorphisms in the epidermal growth factor receptor gene promoter |
| CN101370945B (zh) | 2005-10-14 | 2011-12-28 | 克利夫兰州立大学 | 用于鉴别大量野生型dna背景中多个dna突变标记的方法 |
| US20070154892A1 (en) | 2005-12-30 | 2007-07-05 | Simon Wain-Hobson | Differential amplification of mutant nucleic acids by PCR in a mixure of nucleic acids |
| WO2007084380A2 (en) | 2006-01-13 | 2007-07-26 | California Institute Of Technology | Compounds and methods for nucleic acid mismatch detection |
| ES2665500T3 (es) | 2010-03-08 | 2018-04-26 | Dana-Farber Cancer Institute, Inc. | Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia |
| US20120164641A1 (en) | 2010-12-22 | 2012-06-28 | Roche Molecular Systems, Inc. | Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene |
| CN103215361A (zh) * | 2013-04-18 | 2013-07-24 | 深圳联合医学科技有限公司 | 等位基因变体检测方法、试剂盒及组合物 |
-
2014
- 2014-11-19 US US14/547,899 patent/US9873908B2/en active Active
- 2014-11-25 WO PCT/EP2014/075460 patent/WO2015078831A1/en not_active Ceased
- 2014-11-25 CA CA2926693A patent/CA2926693C/en active Active
- 2014-11-25 EP EP14805538.7A patent/EP3074529B1/en active Active
- 2014-11-25 CN CN201480064597.4A patent/CN105745336B/zh active Active
- 2014-11-25 JP JP2016521352A patent/JP6553598B2/ja active Active
- 2014-11-25 ES ES14805538.7T patent/ES2676062T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3074529B1 (en) | 2018-05-02 |
| JP2016539624A (ja) | 2016-12-22 |
| CN105745336B (zh) | 2019-03-01 |
| US9873908B2 (en) | 2018-01-23 |
| JP6553598B2 (ja) | 2019-07-31 |
| EP3074529A1 (en) | 2016-10-05 |
| US20150147760A1 (en) | 2015-05-28 |
| CA2926693A1 (en) | 2015-06-04 |
| CA2926693C (en) | 2018-06-19 |
| WO2015078831A1 (en) | 2015-06-04 |
| CN105745336A (zh) | 2016-07-06 |
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