JP6553598B2 - 混合物から変異された核酸の富化方法 - Google Patents

混合物から変異された核酸の富化方法 Download PDF

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JP6553598B2
JP6553598B2 JP2016521352A JP2016521352A JP6553598B2 JP 6553598 B2 JP6553598 B2 JP 6553598B2 JP 2016521352 A JP2016521352 A JP 2016521352A JP 2016521352 A JP2016521352 A JP 2016521352A JP 6553598 B2 JP6553598 B2 JP 6553598B2
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nucleic acid
double
oligonucleotide
target nucleic
allele
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JP2016539624A5 (enExample
JP2016539624A (ja
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シェーンブルナー ナンシー
シェーンブルナー ナンシー
グプタ アマル
グプタ アマル
ジャンセン ケビン
ジャンセン ケビン
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2016521352A 2013-11-27 2014-11-25 混合物から変異された核酸の富化方法 Active JP6553598B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361909587P 2013-11-27 2013-11-27
US61/909,587 2013-11-27
PCT/EP2014/075460 WO2015078831A1 (en) 2013-11-27 2014-11-25 Methods for the enrichment of mutated nucleic acid from a mixture

Publications (3)

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JP2016539624A JP2016539624A (ja) 2016-12-22
JP2016539624A5 JP2016539624A5 (enExample) 2019-02-28
JP6553598B2 true JP6553598B2 (ja) 2019-07-31

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JP2016521352A Active JP6553598B2 (ja) 2013-11-27 2014-11-25 混合物から変異された核酸の富化方法

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US (1) US9873908B2 (enExample)
EP (1) EP3074529B1 (enExample)
JP (1) JP6553598B2 (enExample)
CN (1) CN105745336B (enExample)
CA (1) CA2926693C (enExample)
ES (1) ES2676062T3 (enExample)
WO (1) WO2015078831A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2665500T3 (es) 2010-03-08 2018-04-26 Dana-Farber Cancer Institute, Inc. Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia
DK2691541T3 (en) 2011-03-31 2018-01-22 Dana Farber Cancer Inst Inc PROCEDURE FOR ENRICHMENT OF SINGLE DRAWED MUTANTS SEQUENCES FROM A MIXTURE OF WILD TYPE AND MUTANTS
EP3907297A1 (en) 2011-04-15 2021-11-10 The Johns Hopkins University Safe sequencing system
US11525163B2 (en) 2012-10-29 2022-12-13 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
JP6941568B2 (ja) 2015-06-24 2021-09-29 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド ヌクレアーゼを使用する野生型ｄｎａの選択的分解および突然変異体対立遺伝子の濃縮
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
CA3046953A1 (en) * 2016-12-12 2018-06-21 Dana Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
SG11202001010UA (en) 2017-08-07 2020-03-30 Univ Johns Hopkins Methods and materials for assessing and treating cancer
US20200399683A1 (en) * 2018-02-26 2020-12-24 Cy Molecular Diagnostics, Inc. Composition and Methods For Affinity Directed Enrichment of Rare Species
CA3103754C (en) 2018-07-19 2022-06-07 Biofidelity Ltd Improved polynucleotide sequence detection method
CA3170345A1 (en) 2020-02-14 2021-08-19 The Johns Hopkins University Methods and materials for assessing nucleic acids
IL307733A (en) * 2021-04-15 2023-12-01 Biofidelity Enrichment and discovery of nucleic acids
CN113774113B (zh) * 2021-09-17 2023-07-04 广州医科大学附属第一医院（广州呼吸中心） 一种富集突变基因序列的方法和应用

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6120992A (en) * 1993-11-04 2000-09-19 Valigene Corporation Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human
WO1997034015A1 (en) 1996-03-15 1997-09-18 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
US6630301B1 (en) 1997-03-14 2003-10-07 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum
US6031098A (en) 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
SE9902741D0 (sv) 1999-07-19 1999-07-19 Boerries Kemper Method for the enrichment of heteroduplexes and its use in mutation detection
US20030082537A1 (en) * 2000-05-23 2003-05-01 Stanton Vincent P. Methods for genetic analysis of DNA to detect sequence variances
US20020132244A1 (en) 2000-09-27 2002-09-19 Xiao-Cheng Li-Sucholeiki Methods for detecting rare polymorphic variants in genomic DNA sequences
US6808884B2 (en) * 2000-12-08 2004-10-26 California Institute Of Technology Methods and compositions for detecting polynucleotide duplex damage and errors
WO2002086196A1 (en) * 2001-04-19 2002-10-31 Rd Chemical Company Copper acid baths, system and method for electroplating high aspect ratio substrates
WO2002086169A1 (en) 2001-04-23 2002-10-31 Dana-Farber Cancer Institute, Inc. Methods for rapid screening of polymorphisms, mutations and methylation
GB2382137A (en) 2001-11-20 2003-05-21 Mats Gullberg Nucleic acid enrichment
CN101056990A (zh) * 2004-03-01 2007-10-17 芝加哥大学 表皮生长因子受体基因启动子的多态性
ATE548469T1 (de) 2005-10-14 2012-03-15 Univ State Cleveland Verfahren zur identifizierung mehrerer mutationen oder methylierungsmarkern in einem dominierenden hintergrund von wildtyp-dna
US20070154892A1 (en) 2005-12-30 2007-07-05 Simon Wain-Hobson Differential amplification of mutant nucleic acids by PCR in a mixure of nucleic acids
WO2007084380A2 (en) 2006-01-13 2007-07-26 California Institute Of Technology Compounds and methods for nucleic acid mismatch detection
ES2665500T3 (es) 2010-03-08 2018-04-26 Dana-Farber Cancer Institute, Inc. Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia
US20120164641A1 (en) 2010-12-22 2012-06-28 Roche Molecular Systems, Inc. Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene
CN103215361A (zh) * 2013-04-18 2013-07-24 深圳联合医学科技有限公司 等位基因变体检测方法、试剂盒及组合物

Also Published As

Publication number Publication date
US9873908B2 (en) 2018-01-23
US20150147760A1 (en) 2015-05-28
EP3074529A1 (en) 2016-10-05
CA2926693C (en) 2018-06-19
CN105745336B (zh) 2019-03-01
ES2676062T3 (es) 2018-07-16
WO2015078831A1 (en) 2015-06-04
CN105745336A (zh) 2016-07-06
EP3074529B1 (en) 2018-05-02
CA2926693A1 (en) 2015-06-04
JP2016539624A (ja) 2016-12-22

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