CA2926693C - Methods for the enrichment of mutated nucleic acid from a mixture - Google Patents

Methods for the enrichment of mutated nucleic acid from a mixture Download PDF

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Publication number
CA2926693C
CA2926693C CA2926693A CA2926693A CA2926693C CA 2926693 C CA2926693 C CA 2926693C CA 2926693 A CA2926693 A CA 2926693A CA 2926693 A CA2926693 A CA 2926693A CA 2926693 C CA2926693 C CA 2926693C
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Canada
Prior art keywords
nucleic acid
strand
mismatch
target nucleic
oligonucleotide
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CA2926693A
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English (en)
French (fr)
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CA2926693A1 (en
Inventor
Nancy Schoenbrunner
Amar Gupta
Kevin JANSSEN
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2926693A 2013-11-27 2014-11-25 Methods for the enrichment of mutated nucleic acid from a mixture Active CA2926693C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361909587P 2013-11-27 2013-11-27
US61/909,587 2013-11-27
PCT/EP2014/075460 WO2015078831A1 (en) 2013-11-27 2014-11-25 Methods for the enrichment of mutated nucleic acid from a mixture

Publications (2)

Publication Number Publication Date
CA2926693A1 CA2926693A1 (en) 2015-06-04
CA2926693C true CA2926693C (en) 2018-06-19

Family

ID=52000821

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2926693A Active CA2926693C (en) 2013-11-27 2014-11-25 Methods for the enrichment of mutated nucleic acid from a mixture

Country Status (7)

Country Link
US (1) US9873908B2 (enExample)
EP (1) EP3074529B1 (enExample)
JP (1) JP6553598B2 (enExample)
CN (1) CN105745336B (enExample)
CA (1) CA2926693C (enExample)
ES (1) ES2676062T3 (enExample)
WO (1) WO2015078831A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5795341B2 (ja) 2010-03-08 2015-10-14 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド 参照ブロック配列によるｆｕｌｌＣＯＬＤ−ＰＣＲ濃縮
US11130992B2 (en) 2011-03-31 2021-09-28 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex COLD-PCR
AU2012242847B2 (en) 2011-04-15 2017-01-19 The Johns Hopkins University Safe sequencing system
ES2701742T3 (es) 2012-10-29 2019-02-25 Univ Johns Hopkins Prueba de Papanicolaou para cánceres de ovario y de endometrio
CA2990846C (en) 2015-06-24 2023-09-26 Dana-Farber Cancer Institute, Inc. Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease
WO2017027653A1 (en) 2015-08-11 2017-02-16 The Johns Hopkins University Assaying ovarian cyst fluid
US11371090B2 (en) 2016-12-12 2022-06-28 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection
CN120400338A (zh) 2017-08-07 2025-08-01 约翰斯霍普金斯大学 用于评估和治疗癌症的方法和材料
EP3746567A4 (en) * 2018-02-26 2021-11-03 CY Molecular Diagnostics, Inc COMPOSITION AND PROCESS FOR AFFINITY-BASED ENRICHMENT OF RARE SPECIES
KR102589502B1 (ko) 2018-07-19 2023-10-13 바이오피델리티 엘티디 개선된 폴리뉴클레오티드 서열 탐지 방법
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
IL295297A (en) 2020-02-14 2022-10-01 Univ Johns Hopkins Methods and materials for assessing nucleic acids
WO2022219408A1 (en) * 2021-04-15 2022-10-20 Biofidelity Nucleic acid enrichment and detection
CN113774113B (zh) * 2021-09-17 2023-07-04 广州医科大学附属第一医院（广州呼吸中心） 一种富集突变基因序列的方法和应用

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6120992A (en) * 1993-11-04 2000-09-19 Valigene Corporation Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human
US6156504A (en) 1996-03-15 2000-12-05 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
US6630301B1 (en) 1997-03-14 2003-10-07 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum
US6031098A (en) 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
SE9902741D0 (sv) 1999-07-19 1999-07-19 Boerries Kemper Method for the enrichment of heteroduplexes and its use in mutation detection
US20030082537A1 (en) * 2000-05-23 2003-05-01 Stanton Vincent P. Methods for genetic analysis of DNA to detect sequence variances
US20020132244A1 (en) 2000-09-27 2002-09-19 Xiao-Cheng Li-Sucholeiki Methods for detecting rare polymorphic variants in genomic DNA sequences
JP2004522739A (ja) * 2000-12-08 2004-07-29 カリフォルニア インスティテュート オブ テクノロジー ポリヌクレオチド二本鎖損傷を検出するための方法および組成物
WO2002086196A1 (en) * 2001-04-19 2002-10-31 Rd Chemical Company Copper acid baths, system and method for electroplating high aspect ratio substrates
WO2002086169A1 (en) 2001-04-23 2002-10-31 Dana-Farber Cancer Institute, Inc. Methods for rapid screening of polymorphisms, mutations and methylation
GB2382137A (en) 2001-11-20 2003-05-21 Mats Gullberg Nucleic acid enrichment
CN101056990A (zh) * 2004-03-01 2007-10-17 芝加哥大学 表皮生长因子受体基因启动子的多态性
CN101370945B (zh) 2005-10-14 2011-12-28 克利夫兰州立大学 用于鉴别大量野生型dna背景中多个dna突变标记的方法
US20070154892A1 (en) 2005-12-30 2007-07-05 Simon Wain-Hobson Differential amplification of mutant nucleic acids by PCR in a mixure of nucleic acids
US7786298B2 (en) 2006-01-13 2010-08-31 California Institute Of Technology Compounds and methods for nucleic acid mismatch detection
JP5795341B2 (ja) 2010-03-08 2015-10-14 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド 参照ブロック配列によるｆｕｌｌＣＯＬＤ−ＰＣＲ濃縮
US20120164641A1 (en) 2010-12-22 2012-06-28 Roche Molecular Systems, Inc. Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene
CN103215361A (zh) * 2013-04-18 2013-07-24 深圳联合医学科技有限公司 等位基因变体检测方法、试剂盒及组合物

Also Published As

Publication number Publication date
JP6553598B2 (ja) 2019-07-31
ES2676062T3 (es) 2018-07-16
US9873908B2 (en) 2018-01-23
CA2926693A1 (en) 2015-06-04
JP2016539624A (ja) 2016-12-22
EP3074529B1 (en) 2018-05-02
US20150147760A1 (en) 2015-05-28
EP3074529A1 (en) 2016-10-05
CN105745336A (zh) 2016-07-06
CN105745336B (zh) 2019-03-01
WO2015078831A1 (en) 2015-06-04

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