ES2665012T3 - Procedimiento y kit para el aislamiento secuencial de especies nucleotídicas de una muestra - Google Patents
Procedimiento y kit para el aislamiento secuencial de especies nucleotídicas de una muestra Download PDFInfo
- Publication number
- ES2665012T3 ES2665012T3 ES11801242.6T ES11801242T ES2665012T3 ES 2665012 T3 ES2665012 T3 ES 2665012T3 ES 11801242 T ES11801242 T ES 11801242T ES 2665012 T3 ES2665012 T3 ES 2665012T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acid
- species
- dna
- buffer
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 67
- 238000002955 isolation Methods 0.000 title claims abstract description 17
- 239000002773 nucleotide Substances 0.000 title description 12
- 125000003729 nucleotide group Chemical group 0.000 title description 12
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 82
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 82
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 82
- 239000007790 solid phase Substances 0.000 claims abstract description 50
- 230000027455 binding Effects 0.000 claims abstract description 32
- 239000012139 lysis buffer Substances 0.000 claims abstract description 31
- 239000006166 lysate Substances 0.000 claims abstract description 30
- 239000012472 biological sample Substances 0.000 claims abstract description 23
- 238000011084 recovery Methods 0.000 claims abstract description 22
- 239000012148 binding buffer Substances 0.000 claims abstract description 20
- 230000009089 cytolysis Effects 0.000 claims abstract description 14
- 238000000926 separation method Methods 0.000 claims abstract description 9
- 230000006862 enzymatic digestion Effects 0.000 claims abstract description 6
- 238000000265 homogenisation Methods 0.000 claims abstract description 3
- 108020004414 DNA Proteins 0.000 claims description 150
- 102000053602 DNA Human genes 0.000 claims description 141
- 229920002477 rna polymer Polymers 0.000 claims description 101
- 239000007787 solid Substances 0.000 claims description 31
- 239000011534 wash buffer Substances 0.000 claims description 29
- 239000000243 solution Substances 0.000 claims description 28
- 230000008569 process Effects 0.000 claims description 25
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 23
- 239000000872 buffer Substances 0.000 claims description 20
- 239000012149 elution buffer Substances 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 15
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims description 14
- 108010067770 Endopeptidase K Proteins 0.000 claims description 13
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 12
- 239000007983 Tris buffer Substances 0.000 claims description 10
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 10
- 108010053770 Deoxyribonucleases Proteins 0.000 claims description 9
- 102000016911 Deoxyribonucleases Human genes 0.000 claims description 9
- 229960004198 guanidine Drugs 0.000 claims description 8
- 150000003839 salts Chemical class 0.000 claims description 8
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 7
- 150000001298 alcohols Chemical class 0.000 claims description 7
- 239000007864 aqueous solution Substances 0.000 claims description 7
- 230000003196 chaotropic effect Effects 0.000 claims description 7
- 230000029087 digestion Effects 0.000 claims description 7
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 7
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 7
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims description 6
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims description 6
- 230000002378 acidificating effect Effects 0.000 claims description 6
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 claims description 6
- -1 pH 7.2 Substances 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 4
- 229910020175 SiOH Inorganic materials 0.000 claims description 3
- 239000013078 crystal Substances 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 108
- 239000000377 silicon dioxide Substances 0.000 description 53
- 241000282414 Homo sapiens Species 0.000 description 39
- 241000894007 species Species 0.000 description 35
- 239000011159 matrix material Substances 0.000 description 33
- 210000001519 tissue Anatomy 0.000 description 27
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 26
- 239000011324 bead Substances 0.000 description 24
- 238000010828 elution Methods 0.000 description 22
- 210000001072 colon Anatomy 0.000 description 15
- 239000000463 material Substances 0.000 description 13
- 239000006249 magnetic particle Substances 0.000 description 12
- 239000000523 sample Substances 0.000 description 11
- 238000005406 washing Methods 0.000 description 11
- 239000012620 biological material Substances 0.000 description 9
- 239000002245 particle Substances 0.000 description 9
- 230000004570 RNA-binding Effects 0.000 description 8
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 8
- 239000001509 sodium citrate Substances 0.000 description 8
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 8
- 229940038773 trisodium citrate Drugs 0.000 description 8
- 238000011109 contamination Methods 0.000 description 7
- 239000011049 pearl Substances 0.000 description 7
- 238000000746 purification Methods 0.000 description 7
- 230000000717 retained effect Effects 0.000 description 7
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 6
- 238000002123 RNA extraction Methods 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 210000000481 breast Anatomy 0.000 description 6
- 238000000605 extraction Methods 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 230000002441 reversible effect Effects 0.000 description 6
- 210000000952 spleen Anatomy 0.000 description 6
- 101000756632 Homo sapiens Actin, cytoplasmic 1 Proteins 0.000 description 5
- 206010028980 Neoplasm Diseases 0.000 description 5
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 5
- 229910000831 Steel Inorganic materials 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 239000012634 fragment Substances 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 5
- 239000012535 impurity Substances 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 210000002741 palatine tonsil Anatomy 0.000 description 5
- 229910052710 silicon Inorganic materials 0.000 description 5
- 239000010703 silicon Substances 0.000 description 5
- 239000010959 steel Substances 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 101000686547 Arabidopsis thaliana 30S ribosomal protein S1, chloroplastic Proteins 0.000 description 4
- 206010006187 Breast cancer Diseases 0.000 description 4
- 208000026310 Breast neoplasm Diseases 0.000 description 4
- 206010009944 Colon cancer Diseases 0.000 description 4
- 230000004568 DNA-binding Effects 0.000 description 4
- 101001125551 Homo sapiens Ribose-phosphate pyrophosphokinase 1 Proteins 0.000 description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- 102100029508 Ribose-phosphate pyrophosphokinase 1 Human genes 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 230000015556 catabolic process Effects 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 208000029742 colonic neoplasm Diseases 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- 238000000227 grinding Methods 0.000 description 4
- 229960000789 guanidine hydrochloride Drugs 0.000 description 4
- 244000052769 pathogen Species 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000010839 reverse transcription Methods 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 239000013049 sediment Substances 0.000 description 4
- 238000004513 sizing Methods 0.000 description 4
- 239000013076 target substance Substances 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 108700024394 Exon Proteins 0.000 description 3
- 108091092584 GDNA Proteins 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 206010044002 Tonsil cancer Diseases 0.000 description 3
- 208000006842 Tonsillar Neoplasms Diseases 0.000 description 3
- 238000013019 agitation Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 210000000265 leukocyte Anatomy 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000003960 organic solvent Substances 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 239000013614 RNA sample Substances 0.000 description 2
- 208000000277 Splenic Neoplasms Diseases 0.000 description 2
- 239000007984 Tris EDTA buffer Substances 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000010836 blood and blood product Substances 0.000 description 2
- 229940125691 blood product Drugs 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 239000003623 enhancer Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000003754 fetus Anatomy 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 238000001821 nucleic acid purification Methods 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 201000002471 spleen cancer Diseases 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- BHNQPLPANNDEGL-UHFFFAOYSA-N 2-(4-octylphenoxy)ethanol Chemical compound CCCCCCCCC1=CC=C(OCCO)C=C1 BHNQPLPANNDEGL-UHFFFAOYSA-N 0.000 description 1
- 108091032955 Bacterial small RNA Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical class NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 101150020418 PRPS1 gene Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- CGNLCCVKSWNSDG-UHFFFAOYSA-N SYBR Green I Chemical compound CN(C)CCCN(CCC)C1=CC(C=C2N(C3=CC=CC=C3S2)C)=C2C=CC=CC2=[N+]1C1=CC=CC=C1 CGNLCCVKSWNSDG-UHFFFAOYSA-N 0.000 description 1
- SAQSTQBVENFSKT-UHFFFAOYSA-M TCA-sodium Chemical compound [Na+].[O-]C(=O)C(Cl)(Cl)Cl SAQSTQBVENFSKT-UHFFFAOYSA-M 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000012062 aqueous buffer Substances 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 201000006491 bone marrow cancer Diseases 0.000 description 1
- 210000002798 bone marrow cell Anatomy 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 108091070501 miRNA Proteins 0.000 description 1
- 239000002679 microRNA Substances 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 238000002205 phenol-chloroform extraction Methods 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 230000006916 protein interaction Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000012776 robust process Methods 0.000 description 1
- 238000007480 sanger sequencing Methods 0.000 description 1
- 235000009518 sodium iodide Nutrition 0.000 description 1
- BAZAXWOYCMUHIX-UHFFFAOYSA-M sodium perchlorate Chemical compound [Na+].[O-]Cl(=O)(=O)=O BAZAXWOYCMUHIX-UHFFFAOYSA-M 0.000 description 1
- 229910001488 sodium perchlorate Inorganic materials 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE1000701 | 2010-06-29 | ||
| SE1000701 | 2010-06-29 | ||
| SE1100480 | 2010-11-22 | ||
| SE1100480 | 2010-11-22 | ||
| PCT/SE2011/050848 WO2012002887A1 (en) | 2010-06-29 | 2011-06-27 | Method and kit for sequential isolation of nucleotide species from a sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2665012T3 true ES2665012T3 (es) | 2018-04-24 |
Family
ID=45402362
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES11801242.6T Active ES2665012T3 (es) | 2010-06-29 | 2011-06-27 | Procedimiento y kit para el aislamiento secuencial de especies nucleotídicas de una muestra |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US8889393B2 (enExample) |
| EP (1) | EP2588609B1 (enExample) |
| DK (1) | DK2588609T3 (enExample) |
| ES (1) | ES2665012T3 (enExample) |
| NO (1) | NO2588609T3 (enExample) |
| PL (1) | PL2588609T3 (enExample) |
| PT (1) | PT2588609T (enExample) |
| WO (1) | WO2012002887A1 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2012253345B2 (en) | 2011-05-12 | 2016-10-20 | Exact Sciences Corporation | Isolation of nucleic acids |
| US8808990B2 (en) | 2011-05-12 | 2014-08-19 | Exact Sciences Corporation | Serial isolation of multiple DNA targets from stool |
| US8993341B2 (en) | 2011-05-12 | 2015-03-31 | Exact Sciences Corporation | Removal of PCR inhibitors |
| US8980107B2 (en) | 2011-05-12 | 2015-03-17 | Exact Sciences Corporation | Spin filter |
| JP6096774B2 (ja) * | 2011-08-12 | 2017-03-15 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 核酸を単離するための方法 |
| US9670479B2 (en) | 2013-03-15 | 2017-06-06 | F Cubed, LLC | Sample preparation device and methods of use |
| AU2014305085A1 (en) * | 2013-08-05 | 2016-02-25 | The Johns Hopkins University | Fabrication of hierarchical silica nanomembranes and uses thereof for solid phase extraction of nucleic acids |
| SG11201609008PA (en) | 2014-04-29 | 2016-11-29 | Accudx Corp | A novel affinity matrix and devices for isolation and purification of rna and dna for point of care molecular devices |
| EP2942394A1 (en) * | 2014-05-09 | 2015-11-11 | Molzym GmbH & Co. KG | New method for isolating microbial DNA |
| US20160010081A1 (en) * | 2014-07-11 | 2016-01-14 | Exact Sciences Corporation | Systems, methods and kits for extracting nucleic acid from digestive fluid |
| WO2016123101A1 (en) | 2015-01-27 | 2016-08-04 | Circulomics Inc. | Hierarchical silica lamella for magnetic nucleic acid extraction |
| US11242518B2 (en) | 2015-09-04 | 2022-02-08 | QIAGEN Sciences, LLP | Methods for co-isolation of nucleic acids and proteins |
| US10704081B2 (en) * | 2015-10-30 | 2020-07-07 | Exact Sciences Development Company, Llc | Multiplex amplification detection assay |
| CN112313344A (zh) | 2018-04-24 | 2021-02-02 | 凯杰科技有限公司 | 从复杂样品中分离核酸并去除抑制剂 |
| WO2019209600A1 (en) | 2018-04-24 | 2019-10-31 | Qiagen Sciences Llc | Biomolecule isolation and inhibitor removal |
| WO2019207168A1 (en) | 2018-04-27 | 2019-10-31 | Qiagen Gmbh | Method for isolating nucleic acids from plant samples |
| AU2019266454B9 (en) | 2018-05-11 | 2025-09-18 | Qiagen Gmbh | Lysis method for plant samples |
| WO2020069385A1 (en) * | 2018-09-28 | 2020-04-02 | Beckman Coulter, Inc. | Isolation of dna and rna from a single sample |
| WO2023198909A1 (en) * | 2022-04-15 | 2023-10-19 | Quantoom Biosciences S.A. | Methods for separation and/or purification of nucleic acids |
| WO2025046144A1 (en) | 2023-09-01 | 2025-03-06 | Qiagen Gmbh | Method for isolating rna from inhibitor rich samples |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5990301A (en) | 1994-02-07 | 1999-11-23 | Qiagen Gmbh | Process for the separation and purification of nucleic acids from biological sources |
| DK1146049T3 (da) | 1994-02-11 | 2005-12-12 | Qiagen Gmbh | Fremgangsmåde til separering af dobbeltstrengede/enkeltstrengede nucleinsyrestrukturer |
| GB9425138D0 (en) * | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| JP2002502856A (ja) * | 1998-02-04 | 2002-01-29 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフトング | 核酸の単離および精製方法 |
| WO2003046146A2 (en) * | 2001-11-28 | 2003-06-05 | Applera Corporation | Compositions and methods of selective nucleic acid isolation |
| CA2473376A1 (en) | 2002-01-16 | 2003-07-31 | Dynal Biotech Asa | Method for isolating nucleic acids and protein from a single sample |
| GB0215185D0 (en) | 2002-07-01 | 2002-08-07 | Genovision As | Binding a target substance |
| WO2004108925A1 (en) * | 2003-06-04 | 2004-12-16 | Qiagen As | Method for sequentially isolating dna and rna from the same nucleic acid-containing sample |
| DE10358137A1 (de) | 2003-12-12 | 2005-07-07 | Merck Patent Gmbh | Verfahren und Kit zur Isolierung von RNA |
| DE102006031764B4 (de) | 2006-07-06 | 2009-10-01 | Aj Innuscreen Gmbh | Verfahren zur parallelen Isolierung doppel- und einzelsträngiger Nukleinsäuren sowie zur selektiven Entfernung doppelsträngiger Nukleinsäuren aus einem Gemisch von doppel- und einzelsträngigen Nukleinsäuren |
| GB2443505B (en) | 2006-09-26 | 2008-12-31 | Ge Healthcare Bio Sciences | Nucleic acid purification method |
| GB2445441B (en) | 2006-09-26 | 2010-06-30 | Ge Healthcare Bio Sciences | Nucleic acid purification method |
| GB2445442A (en) | 2006-09-26 | 2008-07-09 | Ge Healthcare Bio Sciences | Nucleic acid purification using anion exchange |
| US8067580B2 (en) | 2008-02-07 | 2011-11-29 | Ge Healthcare Bio-Sciences Corp. | Isolation of DNA, RNA and protein from a single sample |
| WO2009134652A1 (en) * | 2008-04-30 | 2009-11-05 | Ge Healthcare Bio-Sciences Corp. | Method for separation of double-stranded and single-stranded nucleic acids |
-
2011
- 2011-06-27 WO PCT/SE2011/050848 patent/WO2012002887A1/en not_active Ceased
- 2011-06-27 DK DK11801242.6T patent/DK2588609T3/en active
- 2011-06-27 US US13/704,739 patent/US8889393B2/en active Active
- 2011-06-27 NO NO11801242A patent/NO2588609T3/no unknown
- 2011-06-27 EP EP11801242.6A patent/EP2588609B1/en active Active
- 2011-06-27 ES ES11801242.6T patent/ES2665012T3/es active Active
- 2011-06-27 PT PT118012426T patent/PT2588609T/pt unknown
- 2011-06-27 PL PL11801242T patent/PL2588609T3/pl unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DK2588609T3 (en) | 2018-03-19 |
| US8889393B2 (en) | 2014-11-18 |
| EP2588609A1 (en) | 2013-05-08 |
| PT2588609T (pt) | 2018-04-02 |
| PL2588609T3 (pl) | 2018-06-29 |
| NO2588609T3 (enExample) | 2018-06-16 |
| EP2588609A4 (en) | 2014-03-26 |
| US20130164819A1 (en) | 2013-06-27 |
| WO2012002887A1 (en) | 2012-01-05 |
| EP2588609B1 (en) | 2018-01-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2665012T3 (es) | Procedimiento y kit para el aislamiento secuencial de especies nucleotídicas de una muestra | |
| ES2950788T3 (es) | Procedimiento para el aislamiento de ácidos nucleicos | |
| ES2470621T3 (es) | Procedimiento para obtener ARN corto as� como kit para el mismo | |
| JP6689251B2 (ja) | Ｒｎａを高収率で単離するための方法 | |
| ES2321492T3 (es) | Aislamiento y purificacion de acidos nucleicos. | |
| ES2793249T3 (es) | Método para aislar moléculas de ARN pequeño | |
| EP1618194B1 (en) | Compositions and methods for using a solid support to purify rna | |
| JP5390772B2 (ja) | 核酸を安定化試薬から精製するための組成物および方法 | |
| AU774810B2 (en) | Mixed-bed solid phase and its use in the isolation of nucleic acids | |
| ES2163865T5 (es) | Aislamiento en fase solida de acidos nucleicos. | |
| EP2066792B1 (en) | Nucleic acid purification method using anion exchange | |
| JP5777221B2 (ja) | 核酸の精製方法及びこの方法の実施に使用する配合物及びキット | |
| ES2654539T3 (es) | Procedimiento para el aislamiento de ADN a partir de muestras biológicas | |
| ES2322841T3 (es) | Aislamiento y purificacion magnetica de acidos nucleicos. | |
| BRPI0606363B1 (pt) | Método para o isolamento e/ou a purificação de ácidos nucleicos ácido nucleico, e, método para a imobilização de ácidos nucleicos em uma matriz | |
| CN104603267A (zh) | 高产量地分离包括小rna的rna的方法 | |
| ES2904624T3 (es) | Método para aislar ácido nucleico altamente puro con partículas magnéticas | |
| ES2965460T3 (es) | Método automatizable para el aislamiento de ácidos nucleicos | |
| ES2729164T3 (es) | Procedimiento para el enriquecimiento de ácidos nucleicos de cadena corta | |
| CN102115739B (zh) | 分离核酸的方法、试剂及套组 | |
| WO2017040992A1 (en) | Methods for co-isolation of nucelic acids and proteins | |
| KR20210132375A (ko) | 자성나노입자와 결합된 산화그래핀을 이용한 핵산의 분리 방법 | |
| US20090088560A1 (en) | Process for Nucleic Acid Purification | |
| ES2350291T3 (es) | Método para aislar ácidos nucleicos que comprende el uso de multímeros de etilenglicol. | |
| AU2004233035B2 (en) | Compositions and methods for using a solid support to purify RNA |