ES2628012T3 - Producción de TNFR-Ig en un cultivo celular de producción a gran escala - Google Patents

Producción de TNFR-Ig en un cultivo celular de producción a gran escala Download PDF

Info

Publication number
ES2628012T3
ES2628012T3 ES05792494T ES05792494T ES2628012T3 ES 2628012 T3 ES2628012 T3 ES 2628012T3 ES 05792494 T ES05792494 T ES 05792494T ES 05792494 T ES05792494 T ES 05792494T ES 2628012 T3 ES2628012 T3 ES 2628012T3
Authority
ES
Spain
Prior art keywords
culture
cumulative
tnfr
cell culture
conditions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES05792494T
Other languages
English (en)
Inventor
Denis Drapeau
Yen-Tung Luan
James R. Mercer
Wenge Wang
Daniel R. Lasko
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Ireland Pharmaceuticals
Original Assignee
Pfizer Ireland Pharmaceuticals
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=35482353&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2628012(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Pfizer Ireland Pharmaceuticals filed Critical Pfizer Ireland Pharmaceuticals
Application granted granted Critical
Publication of ES2628012T3 publication Critical patent/ES2628012T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Un procedimiento de producción de una proteína de fusión de inmunoglobulina con el receptor del factor de necrosis tumoral (TNFR-Ig) en un cultivo celular de producción a gran escala que comprende las etapas de: proporcionar un cultivo celular que comprende; células de mamífero que contienen un gen que codifica una TNFR-Ig de interés, gen que se expresa en condiciones de cultivo celular; y un medio que contiene glutamina, que tiene (i) una cantidad acumulativa de aminoácidos por unidad de volumen mayor de 70 mM; (ii) una relación molar de glutamina acumulativa a asparagina acumulativa de menos de 2; (iii) una relación molar de glutamina acumulativa a aminoácidos totales acumulativos de menos de 0,2; y (iv) una cantidad acumulativa combinada de glutamina y asparagina por unidad de volumen de más de 16 mM; mantener dicho cultivo en una fase de crecimiento inicial en un primer conjunto de condiciones de cultivo durante un primer periodo de tiempo suficiente para permitir que dichas células se reproduzcan hasta una densidad celular viable dentro de un intervalo de un 20 % - 80 % de la máxima densidad posible de células viables si dicho cultivo se mantuviera en el primer conjunto de condiciones de cultivo; cambiar al menos una de las condiciones de cultivo, de modo que se aplique un segundo conjunto de condiciones de cultivo; mantener dicho cultivo durante un segundo periodo de tiempo en el segundo conjunto de condiciones y durante un segundo periodo de tiempo de modo que se acumule TNFR-Ig en el cultivo celular
ES05792494T 2004-08-27 2005-08-26 Producción de TNFR-Ig en un cultivo celular de producción a gran escala Active ES2628012T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US60494104P 2004-08-27 2004-08-27
US60509704P 2004-08-27 2004-08-27
US60507404P 2004-08-27 2004-08-27
PCT/US2005/030437 WO2006026445A1 (en) 2004-08-27 2005-08-26 Production of polypeptides

Publications (1)

Publication Number Publication Date
ES2628012T3 true ES2628012T3 (es) 2017-08-01

Family

ID=35482353

Family Applications (1)

Application Number Title Priority Date Filing Date
ES05792494T Active ES2628012T3 (es) 2004-08-27 2005-08-26 Producción de TNFR-Ig en un cultivo celular de producción a gran escala

Country Status (20)

Country Link
US (1) US7294484B2 (es)
EP (2) EP1789571B1 (es)
JP (1) JP4864892B2 (es)
KR (2) KR101158447B1 (es)
CN (2) CN101048511B (es)
AR (2) AR051017A1 (es)
AU (1) AU2005280034B2 (es)
BR (1) BRPI0514703A (es)
CA (1) CA2578141C (es)
CR (1) CR8997A (es)
EC (1) ECSP077353A (es)
EG (1) EG26462A (es)
ES (1) ES2628012T3 (es)
IL (1) IL181589A0 (es)
MX (1) MX2007002380A (es)
NO (1) NO342433B1 (es)
SG (2) SG158092A1 (es)
TW (1) TWI384069B (es)
WO (1) WO2006026445A1 (es)
ZA (1) ZA200702483B (es)

Families Citing this family (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998008934A1 (en) * 1996-08-30 1998-03-05 Life Technologies, Inc. Serum-free mammalian cell culture medium, and uses thereof
TWI364458B (en) 2004-08-27 2012-05-21 Wyeth Res Ireland Ltd Production of tnfr-lg
TWI384069B (zh) 2004-08-27 2013-02-01 Pfizer Ireland Pharmaceuticals 多胜肽之製法
TWI374935B (en) 2004-08-27 2012-10-21 Pfizer Ireland Pharmaceuticals Production of α-abeta
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
HUE038947T2 (hu) * 2005-08-26 2018-12-28 Ares Trading Sa Eljárás glikozilált béta-interferon elõállítására
AR058140A1 (es) * 2005-10-24 2008-01-23 Wyeth Corp Metodo de produccion proteica utilizando compuestos anti-senescencia
US20070231895A1 (en) * 2005-11-02 2007-10-04 Lee Gene W Methods for adapting mammalian cells
WO2007065918A2 (en) * 2005-12-09 2007-06-14 Ares Trading S.A. Method for purifying fsh or a fsh mutant
KR101423344B1 (ko) 2006-01-04 2014-07-30 백스터 인터내셔널 인코포레이티드 올리고펩티드-무함유 세포 배양 배지를 이용하여 단백질을 발현시키는 방법
CN101496387B (zh) 2006-03-06 2012-09-05 思科技术公司 用于移动无线网络中的接入认证的系统和方法
ES2668212T3 (es) * 2006-07-13 2018-05-17 Wyeth Llc Producción de factor de coagulación IX con patrón de glucosilación mejorado
SG10201510384UA (en) 2006-09-13 2016-01-28 Abbvie Inc Cell culture improvements
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
CA2666317C (en) * 2006-11-03 2013-08-06 Wyeth Glycolysis-inhibiting substances in cell culture
CA2668771C (en) * 2006-11-08 2016-03-15 Wyeth Rationally designed media for cell culture
RU2009132986A (ru) * 2007-03-02 2011-04-10 Уайт (Us) Применение меди и глутамата в культуре клеток для производства полипептидов
TW200902708A (en) * 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
DK2154244T3 (en) 2007-04-26 2017-06-12 Chugai Pharmaceutical Co Ltd CELL CULTIVATION PROCEDURE WHEN AN ACID-ENRICHED MEDIUM IS USED
US20090042253A1 (en) 2007-08-09 2009-02-12 Wyeth Use of perfusion to enhance production of fed-batch cell culture in bioreactors
US8319819B2 (en) 2008-03-26 2012-11-27 Cisco Technology, Inc. Virtual round-table videoconference
AU2009236187B2 (en) * 2008-04-17 2012-08-02 Wyeth Llc Methods for enhanced production of bone morphogenetic proteins
CA2734922C (en) 2008-09-15 2020-01-14 Laetitia Malphettes Compositions comprising polynucleotides comprising hybrid osmo-responsive transcriptional regulatory elements and methods for producing a protein under conditions of hyperosmolarity
US8694658B2 (en) 2008-09-19 2014-04-08 Cisco Technology, Inc. System and method for enabling communication sessions in a network environment
MX2011004200A (es) 2008-10-20 2011-05-24 Abbott Lab Aislamento y purificacion de anticuerpos usando la cromatografia de afinidad de proteina a.
WO2010048192A2 (en) 2008-10-20 2010-04-29 Abbott Laboratories Viral inactivation during purification of antibodies
US8580554B2 (en) 2009-07-31 2013-11-12 Baxter International Inc. Method of producing a polypeptide or virus of interest in a continuous cell culture
LT2464725T (lt) 2009-08-11 2020-06-10 F. Hoffmann-La Roche Ag Baltymų gamyba ląstelių mitybinėje terpėje, kurioje nėra glutamino
GB0917647D0 (en) * 2009-10-08 2009-11-25 Glaxosmithkline Biolog Sa Expression system
CA2787016C (en) * 2010-01-13 2018-01-23 Apt Therapeutics, Inc. Therapeutic apyrase constructs, apyrase agents, and production methods
US20110262965A1 (en) 2010-04-23 2011-10-27 Life Technologies Corporation Cell culture medium comprising small peptides
CN102858953B (zh) 2010-04-26 2015-09-09 诺瓦提斯公司 改进的细胞培养基
PL2563904T3 (pl) * 2010-04-26 2015-06-30 Novartis Ag Udoskonalona pożywka do hodowli komórkowej
CN107254499B (zh) 2010-04-26 2021-09-17 诺华股份有限公司 改进的细胞培养方法
IL286298B (en) 2010-07-08 2022-08-01 Baxalta Inc A method to generate high molecular weight substituted vwf in cell culture
EP2601287B1 (en) 2010-08-05 2015-01-07 Amgen Inc. Dipeptides to enhance yield and viability from cell cultures
CA2807607A1 (en) 2010-08-20 2012-02-23 Wyeth Llc Cell culture of growth factor-free adapted cells
CN102382794B (zh) * 2010-09-01 2013-09-18 山东新时代药业有限公司 一种哺乳动物细胞的灌流培养方法
US8599865B2 (en) 2010-10-26 2013-12-03 Cisco Technology, Inc. System and method for provisioning flows in a mobile network environment
US9338394B2 (en) 2010-11-15 2016-05-10 Cisco Technology, Inc. System and method for providing enhanced audio in a video environment
US8730297B2 (en) 2010-11-15 2014-05-20 Cisco Technology, Inc. System and method for providing camera functions in a video environment
US9143725B2 (en) 2010-11-15 2015-09-22 Cisco Technology, Inc. System and method for providing enhanced graphics in a video environment
US9062106B2 (en) 2011-04-27 2015-06-23 Abbvie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
ES2560470T3 (es) 2011-04-29 2016-02-19 Biocon Research Limited Un método para reducir la heterogeneidad de anticuerpos y un proceso de producción de dichos anticuerpos
LT2837680T (lt) * 2011-07-01 2020-07-10 Amgen Inc. Žinduolių ląstelių kultūra
US8682087B2 (en) 2011-12-19 2014-03-25 Cisco Technology, Inc. System and method for depth-guided image filtering in a video conference environment
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US20130281355A1 (en) * 2012-04-24 2013-10-24 Genentech, Inc. Cell culture compositions and methods for polypeptide production
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
BR112015004467A2 (pt) 2012-09-02 2017-03-21 Abbvie Inc método para controlar a heterogeneidade de proteínas
AU2013381687A1 (en) 2013-03-12 2015-09-24 Abbvie Inc. Human antibodies that bind human TNF-alpha and methods of preparing the same
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
US9217168B2 (en) 2013-03-14 2015-12-22 Momenta Pharmaceuticals, Inc. Methods of cell culture
AR095196A1 (es) 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
IN2013MU02285A (es) * 2013-07-06 2015-06-19 Cadila Healthcare Ltd
WO2015051293A2 (en) 2013-10-04 2015-04-09 Abbvie, Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US11390663B2 (en) * 2013-10-11 2022-07-19 Regeneron Pharmaceuticals, Inc. Metabolically optimized cell culture
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
EP3083933A1 (en) * 2013-12-20 2016-10-26 Biogen MA Inc. Use of perfusion seed cultures to improve biopharmaceutical fed-batch production capacity and product quality
BR112016017660B1 (pt) 2014-02-27 2022-04-19 F. Hoffmann-La Roche Ag Método para a produção de uma glicoproteína recombinante
EP3119876A1 (en) 2014-03-19 2017-01-25 Pfizer Inc. Method of cell culture
TW202246486A (zh) * 2014-06-09 2022-12-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
SG11201704351WA (en) 2014-12-01 2017-06-29 Amgen Inc Process for manipulating the level of glycan content of a glycoprotein
CN107922919A (zh) * 2015-05-29 2018-04-17 勃林格殷格翰国际公司 连续培养中的细胞控制的灌注
TW202330904A (zh) 2015-08-04 2023-08-01 美商再生元醫藥公司 補充牛磺酸之細胞培養基及用法
WO2017051347A2 (en) 2015-09-23 2017-03-30 Pfizer Inc. Cells and method of cell culture
CN108473945A (zh) 2015-11-17 2018-08-31 辉瑞公司 用于在细菌细胞培养物中生产多糖的培养基和发酵方法
JP7253923B2 (ja) * 2016-04-05 2023-04-07 ファイザー・インク 細胞培養プロセス
EP3372670B1 (en) * 2017-03-09 2019-02-27 Evonik Technochemie GmbH Culture media comprising n-acyl-x-glutamine dipeptides
KR102190790B1 (ko) * 2018-10-19 2020-12-15 주식회사 프로젠 변형된 egf 단백질의 생산 방법
US11339197B2 (en) 2017-10-23 2022-05-24 Progen Co., Ltd. Modified EGF protein, production method therefor, and use thereof
JP7471220B2 (ja) * 2017-11-30 2024-04-19 エフ・ホフマン－ラ・ロシュ・アクチェンゲゼルシャフト 哺乳動物細胞の培養方法
MX2020011614A (es) 2018-05-01 2020-12-09 Amgen Inc Anticuerpos con perfiles de glicanos modulados.
CN113015805A (zh) 2018-11-13 2021-06-22 詹森生物科技公司 抗cd38抗体产生期间的痕量金属的控制
US20220073942A1 (en) 2018-12-06 2022-03-10 Pfizer Inc. Cells with reduced inhibitor production and methods of use thereof
CN113025648B (zh) * 2019-12-09 2023-06-02 广东菲鹏制药股份有限公司 Expi293细胞瞬时表达目标蛋白的方法及其应用
MX2023002417A (es) 2020-08-31 2023-03-22 Regeneron Pharma Estrategias de suministro de asparagina para mejorar el rendimiento del cultivo celular y mitigar las variantes de secuencia de asparagina.
JP2022060169A (ja) 2020-10-02 2022-04-14 ファイザー・インク Ｒｓｖ ｆタンパク質生産のための細胞培養工程
US12031151B2 (en) 2021-01-20 2024-07-09 Regeneron Pharmaceuticals, Inc. Methods of improving protein titer in cell culture
WO2022254319A1 (en) 2021-06-01 2022-12-08 Pfizer Inc. Cell culture method for producing sfgfr3 polypeptide
IL311248A (en) 2021-10-07 2024-05-01 Regeneron Pharma PH meter calibration and repair
WO2023059800A2 (en) 2021-10-07 2023-04-13 Regeneron Pharmaceuticals, Inc. Systems and methods of ph modeling and control
WO2023167863A1 (en) 2022-03-02 2023-09-07 Regeneron Pharmaceuticals, Inc. Manufacturing process for high titer antibody
US20240010709A1 (en) 2022-05-25 2024-01-11 Genentech, Inc. Methods for preparing mammalian cells for perfusion cell culture

Family Cites Families (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4522811A (en) 1982-07-08 1985-06-11 Syntex (U.S.A.) Inc. Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides
US4713339A (en) 1983-01-19 1987-12-15 Genentech, Inc. Polycistronic expression vector construction
AU2353384A (en) 1983-01-19 1984-07-26 Genentech Inc. Amplification in eukaryotic host cells
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
JPS6147500A (ja) 1984-08-15 1986-03-07 Res Dev Corp Of Japan キメラモノクロ−ナル抗体及びその製造法
EP0173494A3 (en) 1984-08-27 1987-11-25 The Board Of Trustees Of The Leland Stanford Junior University Chimeric receptors by dna splicing and expression
GB8422238D0 (en) 1984-09-03 1984-10-10 Neuberger M S Chimeric proteins
US5672502A (en) 1985-06-28 1997-09-30 Celltech Therapeutics Limited Animal cell culture
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
US6048728A (en) 1988-09-23 2000-04-11 Chiron Corporation Cell culture medium for enhanced cell growth, culture longevity, and product expression
US6291159B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for producing polymers having a preselected activity
US5605690A (en) 1989-09-05 1997-02-25 Immunex Corporation Methods of lowering active TNF-α levels in mammals using tumor necrosis factor receptor
US5395760A (en) 1989-09-05 1995-03-07 Immunex Corporation DNA encoding tumor necrosis factor-α and -β receptors
NZ235148A (en) 1989-09-05 1991-12-23 Immunex Corp Tumour necrosis factor receptor protein and dna sequences
JPH0396383A (ja) 1989-09-08 1991-04-22 Riso Kagaku Corp 画像形成装置
EP1132471A3 (de) 1989-09-12 2001-11-28 F. Hoffmann-La Roche Ag TNF-bindende Proteine
ES2118066T3 (es) 1989-10-05 1998-09-16 Optein Inc Sintesis y aislamiento, exentos de celulas, de nuevos genes y polipeptidos.
US6657103B1 (en) 1990-01-12 2003-12-02 Abgenix, Inc. Human antibodies derived from immunized xenomice
US5538983A (en) 1990-05-16 1996-07-23 The Rockefeller University Method of treating amyloidosis by modulation of calcium
US5156964A (en) 1990-08-16 1992-10-20 Cetus Corporation Methods for adapting cells for increased product production through exposure to ammonia
US5122469A (en) * 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
GB9021679D0 (en) 1990-10-05 1990-11-21 Gorman Scott David Antibody preparation
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
GB2251249B (en) 1990-12-28 1995-06-21 Mogam Biotech Res Inst High-density medium for animal cell culture
GB9118664D0 (en) 1991-08-30 1991-10-16 Celltech Ltd Cell culture
US5447851B1 (en) 1992-04-02 1999-07-06 Univ Texas System Board Of Dna encoding a chimeric polypeptide comprising the extracellular domain of tnf receptor fused to igg vectors and host cells
US6607884B1 (en) * 1993-03-19 2003-08-19 The Johns Hopkins University School Of Medicine Methods of detecting growth differentiation factor-8
DE4313620A1 (de) * 1993-04-26 1994-10-27 Biotechnolog Forschung Gmbh Hamsterzellinien und Verfahren zur Glykoproteingewinnung
JP3504963B2 (ja) 1993-10-22 2004-03-08 智靖 羅 抗ヒト高親和性ＩｇＥ受容体モノクローナル抗体に係るアミノ酸配列をコードするＤＮＡ断片
US6310185B1 (en) 1994-03-08 2001-10-30 Memorial Sloan Kettering Cancer Center Recombinant human anti-Lewis Y antibodies
US5856179A (en) 1994-03-10 1999-01-05 Genentech, Inc. Polypeptide production in animal cell culture
US5589154A (en) 1994-11-22 1996-12-31 Rutgers, The State University Of New Jersey Methods for the prevention or treatment of vascular hemorrhaging and Alzheimer's disease
US5705364A (en) 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
US5721121A (en) 1995-06-06 1998-02-24 Genentech, Inc. Mammalian cell culture process for producing a tumor necrosis factor receptor immunoglobulin chimeric protein
US6656466B1 (en) * 1995-06-06 2003-12-02 Genetech, Inc. Human tumor necrosis factor—immunoglobulin(TNFR1-IgG1) chimera composition
JP4306813B2 (ja) 1995-09-19 2009-08-05 アスビオファーマ株式会社 動物細胞の新規培養方法
US20020012991A1 (en) * 1997-04-07 2002-01-31 Florence Chua Nee Ho Kit Fong Cell culture media for enhanced protein production
US7964192B1 (en) 1997-12-02 2011-06-21 Janssen Alzheimer Immunotherapy Prevention and treatment of amyloidgenic disease
EP1150688A4 (en) 1998-10-19 2004-06-16 Yeda Res & Dev TREATING SYSTEMIC LUPUS ERYTHEMATODES BY REGULATING THE AUTOIMMUNE RESPONSE TO AUTOANTIGENS
PE20020574A1 (es) 2000-12-06 2002-07-02 Wyeth Corp Anticuerpos humanizados que reconocen el peptido amiloideo beta
TWI329129B (en) 2001-02-08 2010-08-21 Wyeth Corp Modified and stabilized gdf propeptides and uses thereof
ES2437875T3 (es) 2001-04-30 2014-01-14 Eli Lilly And Company Anticuerpos humanizados que reconocen el péptido beta-amiloide
ES2312569T3 (es) 2001-04-30 2009-03-01 Eli Lilly And Company Anticuerpos humanizados.
CA2447791A1 (en) 2001-06-13 2002-12-19 Neslihan Delacruz Methods of culturing animal cells and polypeptide production in animal cells
US7320789B2 (en) 2001-09-26 2008-01-22 Wyeth Antibody inhibitors of GDF-8 and uses thereof
CA2476654A1 (en) 2002-02-21 2003-09-04 Wyeth Follistatin domain containing proteins
MXPA04008150A (es) 2002-02-21 2005-06-17 Wyeth Corp Gasp1: una proteina que contiene dominio de folistatina.
MY139983A (en) 2002-03-12 2009-11-30 Janssen Alzheimer Immunotherap Humanized antibodies that recognize beta amyloid peptide
DK1507556T3 (en) 2002-05-02 2016-09-12 Wyeth Holdings Llc Calicheamicin derivative-carrier conjugates
US6924124B1 (en) 2002-08-23 2005-08-02 Immunex Corporation Feeding strategies for cell culture
AR047392A1 (es) 2002-10-22 2006-01-18 Wyeth Corp Neutralizacion de anticuerpos contra gdf 8 y su uso para tales fines
US20040223966A1 (en) 2002-10-25 2004-11-11 Wolfman Neil M. ActRIIB fusion polypeptides and uses therefor
TWI384069B (zh) 2004-08-27 2013-02-01 Pfizer Ireland Pharmaceuticals 多胜肽之製法
TWI374935B (en) 2004-08-27 2012-10-21 Pfizer Ireland Pharmaceuticals Production of α-abeta
TWI364458B (en) * 2004-08-27 2012-05-21 Wyeth Res Ireland Ltd Production of tnfr-lg

Also Published As

Publication number Publication date
CN102827905A (zh) 2012-12-19
CN102827905B (zh) 2016-08-31
BRPI0514703A (pt) 2008-06-24
ZA200702483B (en) 2018-11-28
US7294484B2 (en) 2007-11-13
SG158092A1 (en) 2010-01-29
CN101048511A (zh) 2007-10-03
CA2578141A1 (en) 2006-03-09
NO342433B1 (no) 2018-05-22
MX2007002380A (es) 2007-06-15
ECSP077353A (es) 2007-05-30
AR086932A2 (es) 2014-01-29
NO20071572L (no) 2007-05-23
IL181589A0 (en) 2007-07-04
EP1789571B1 (en) 2017-04-26
WO2006026445A1 (en) 2006-03-09
AU2005280034A1 (en) 2006-03-09
CA2578141C (en) 2013-01-08
EG26462A (en) 2013-11-13
CR8997A (es) 2007-08-28
SG190674A1 (en) 2013-06-28
EP2357250B1 (en) 2021-10-06
TWI384069B (zh) 2013-02-01
EP2357250A2 (en) 2011-08-17
AR051017A1 (es) 2006-12-13
AU2005280034B2 (en) 2012-06-21
EP1789571A1 (en) 2007-05-30
KR101158447B1 (ko) 2012-06-20
JP2008511329A (ja) 2008-04-17
KR20070085220A (ko) 2007-08-27
JP4864892B2 (ja) 2012-02-01
EP2357250A3 (en) 2014-07-02
US20060121568A1 (en) 2006-06-08
KR20110036780A (ko) 2011-04-08
CN101048511B (zh) 2012-07-18

Similar Documents

Publication Publication Date Title
ES2628012T3 (es) Producción de TNFR-Ig en un cultivo celular de producción a gran escala
PE20060715A1 (es) Proceso de produccion de anti - abeta
RU2009138226A (ru) Способы производства белка с использованием соединений, препятствующих старению
JP4165715B2 (ja) 多重光生物反応器及びそれを利用した光合成微生物培養方法
AR110864A2 (es) Procesos para cultivos celulares
RU2008152449A (ru) Получение гликопротеинов
AR050537A1 (es) Produccion de proteina de fusion tnfr-ig
AR111785A2 (es) Método in vitro de producción de eritrocitos
DE60323468D1 (de) Vögelzell-linien und deren verwendung zur herstellung interessanter biologischer substanzen
EP2360235A1 (en) Photobioreactor for a large-scale marine microalgal culture using half-transmitting film
RU2010128902A (ru) Композиции и способы получения изопрена
ATE491156T1 (de) Bio-elektrochemisches verfahren zur herstellung von wasserstoff
UA92157C2 (ru) Способ продуцирования гормона роста
WO2011072088A3 (en) Improved methods of cell culture for adoptive cell therapy
DK1551954T3 (da) Cellekultur
RU2009132986A (ru) Применение меди и глутамата в культуре клеток для производства полипептидов
AR063623A1 (es) Medios racionalmente disenados para cultivo celular
WO2008136398A1 (ja) 高濃度アミノ酸含有培地を用いた細胞の培養方法
AR084998A1 (es) Metodo de fermentacion de un sustrato gaseoso que comprende hidrogeno
EP1698690A4 (en) BASE MEDIUM FOR CULTIVATING ES CELLS
CN109628950A (zh) 一种微生物电解池原位回收高纯度鸟粪石的方法
US20140170743A1 (en) Photobioreactor for culturing microalgae
WO2002050251A3 (en) A device and method for seed-train expansion of mammalian cells
CN204413401U (zh) 用于在金刚石表层下形成非金刚石层的装置
Hopkinson Hollow fiber cell culture: applications in industry