ES2624871T3 - Ensayos mejorados de la actividad de ADN polimerasa y métodos que facilitan la detección de microbios viables - Google Patents
Ensayos mejorados de la actividad de ADN polimerasa y métodos que facilitan la detección de microbios viables Download PDFInfo
- Publication number
- ES2624871T3 ES2624871T3 ES13733757.2T ES13733757T ES2624871T3 ES 2624871 T3 ES2624871 T3 ES 2624871T3 ES 13733757 T ES13733757 T ES 13733757T ES 2624871 T3 ES2624871 T3 ES 2624871T3
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- dna polymerase
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- pcr
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- dna
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- G01N2333/91205—Phosphotransferases in general
- G01N2333/91245—Nucleotidyltransferases (2.7.7)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Toxicology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261583568P | 2012-01-05 | 2012-01-05 | |
| US201261583568P | 2012-01-05 | ||
| PCT/US2013/020180 WO2013103744A1 (en) | 2012-01-05 | 2013-01-03 | Improved dna polymerase activity assays and methods enabling detection of viable microbes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2624871T3 true ES2624871T3 (es) | 2017-07-17 |
Family
ID=48745412
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES13733757.2T Active ES2624871T3 (es) | 2012-01-05 | 2013-01-03 | Ensayos mejorados de la actividad de ADN polimerasa y métodos que facilitan la detección de microbios viables |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9708651B2 (enExample) |
| EP (1) | EP2800822B1 (enExample) |
| JP (1) | JP6388540B2 (enExample) |
| KR (1) | KR20150005511A (enExample) |
| CN (1) | CN104066853B (enExample) |
| AU (1) | AU2013206888A1 (enExample) |
| CA (1) | CA2897010C (enExample) |
| ES (1) | ES2624871T3 (enExample) |
| IN (1) | IN2014DN06516A (enExample) |
| WO (1) | WO2013103744A1 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201412316D0 (en) * | 2014-07-10 | 2014-08-27 | Momentum Bioscience Ltd | Detecting the absence of micro-organisms |
| CN107541508A (zh) * | 2016-06-24 | 2018-01-05 | 广州康昕瑞基因健康科技有限公司 | 模板‑引物核酸分子、聚合酶活性测定方法及试剂盒 |
| CN106987643A (zh) * | 2017-05-05 | 2017-07-28 | 广州和实生物技术有限公司 | 一种 Taq DNA 聚合酶活性检测方法 |
| GB201805479D0 (en) | 2018-04-03 | 2018-05-16 | Momentum Bioscience Ltd | Microorganism separation and detection |
| CN109609606B (zh) * | 2018-11-28 | 2023-03-31 | 成都博奥晶芯生物科技有限公司 | 一种dna聚合酶的相对酶活的测定方法 |
| GB201914538D0 (en) | 2019-10-08 | 2019-11-20 | Momentum Bioscience Ltd | Microorganism capture from antimicrobial-containing solution |
| KR102441122B1 (ko) * | 2020-05-11 | 2022-09-07 | 주식회사 엔지노믹스 | 폴리머라제 활성 측정 방법 |
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| US20040005555A1 (en) * | 2000-08-31 | 2004-01-08 | Rothman Richard E. | Molecular diagnosis of bactermia |
| WO2006071770A2 (en) * | 2004-12-23 | 2006-07-06 | I-Stat Corporation | Molecular diagnostics system and methods |
| GB0513535D0 (en) * | 2005-07-01 | 2005-08-10 | Iseao Technologies Ltd | Improved methods of detecting microbes |
| US20080113349A1 (en) * | 2006-11-03 | 2008-05-15 | Pranvera Ikonomi | Method for detecting the presence of mammalian organisms using specific cytochrome c oxidase I (COI) and/or cytochrome b subsequences by a PCR based assay |
| CA2692800A1 (en) * | 2007-07-09 | 2009-01-15 | Christopher John Stanley | Detection of micro-organisms based on their nad-dependent dna ligase activity |
| JP2011188855A (ja) * | 2010-03-11 | 2011-09-29 | Samsung Techwin Co Ltd | 切断可能なキメラプローブを使用したサルモネラのリアルタイム検出方法 |
| NZ701866A (en) * | 2010-04-16 | 2016-07-29 | Zeus Scientific Inc | Methods for measuring enzyme activity useful in determining cell viability in non-purified samples |
| KR20150009539A (ko) * | 2012-04-12 | 2015-01-26 | 제우스 사이언티픽, 아이엔씨. | 어떤 중합효소 연장 활성의 민감한, 정량적 측정에 유용한 중합효소 활성을 측정하고 생육성 세포의 존재를 결정하는 방법 |
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