ES2624276T3 - Un método para la expresión transitoria de ácidos nucleicos en plantas - Google Patents

Un método para la expresión transitoria de ácidos nucleicos en plantas Download PDF

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Publication number
ES2624276T3
ES2624276T3 ES10786895.2T ES10786895T ES2624276T3 ES 2624276 T3 ES2624276 T3 ES 2624276T3 ES 10786895 T ES10786895 T ES 10786895T ES 2624276 T3 ES2624276 T3 ES 2624276T3
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plant
gene
expression
promoter
sequence
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English (en)
Spanish (es)
Inventor
Kasi Azhakanandam
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Syngenta Participations AG
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Syngenta Participations AG
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES10786895.2T 2009-06-11 2010-06-11 Un método para la expresión transitoria de ácidos nucleicos en plantas Active ES2624276T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18602509P 2009-06-11 2009-06-11
US186025P 2009-06-11
PCT/US2010/038271 WO2010144775A1 (en) 2009-06-11 2010-06-11 A method for the transient expression of nucleic acids in plants

Publications (1)

Publication Number Publication Date
ES2624276T3 true ES2624276T3 (es) 2017-07-13

Family

ID=43307600

Family Applications (1)

Application Number Title Priority Date Filing Date
ES10786895.2T Active ES2624276T3 (es) 2009-06-11 2010-06-11 Un método para la expresión transitoria de ácidos nucleicos en plantas

Country Status (10)

Country Link
US (2) US8642839B2 (de)
EP (1) EP2440035B1 (de)
CN (1) CN102802405A (de)
AU (1) AU2010260019B2 (de)
BR (1) BR112012000302A2 (de)
CA (1) CA2764570C (de)
ES (1) ES2624276T3 (de)
MX (2) MX354857B (de)
PT (1) PT2440035T (de)
WO (1) WO2010144775A1 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2785845A1 (en) * 2010-01-05 2011-07-14 Syngenta Participations Ag Constitutive synthetic plant promoters and methods of use
JP5472089B2 (ja) * 2010-12-28 2014-04-16 トヨタ自動車株式会社 光合成組織における遺伝子発現制御に関わるdna
WO2012159891A1 (en) 2011-05-20 2012-11-29 Syngenta Participations Ag Endosperm-specific plant promoters and uses therefor
WO2013012788A2 (en) 2011-07-15 2013-01-24 Syngenta Participations Ag Methods of increasing yield and stress tolerance in a plant
EP2647715A1 (de) * 2012-04-03 2013-10-09 Nomad Bioscience GmbH Agrobakterie zur transienten Transfektion ganzer Pflanzen
AU2014278519B2 (en) 2013-06-11 2020-09-10 Syngenta Crop Protection Ag Methods for generating transgenic plants
TW201527315A (zh) * 2013-12-31 2015-07-16 Dow Agrosciences Llc 新穎玉米泛素啓動子(四)
TW201527312A (zh) * 2013-12-31 2015-07-16 Dow Agrosciences Llc 新穎玉米泛素啓動子(一)
CA2954172A1 (en) * 2014-07-10 2016-01-14 Benson Hill Biosystems, Inc. Compositions and methods for increasing plant growth and yield
EP3167392A4 (de) 2014-07-11 2018-08-01 Matatu Inc. Verwendung von darmmikrobiom als prädiktor des wachstums oder der gesundheit von tieren
AU2020369599A1 (en) 2019-10-23 2022-05-12 Monsanto Technology Llc Compositions and methods for RNA-templated editing in plants
CA3160186A1 (en) * 2019-11-05 2021-05-14 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
MX2022009581A (es) * 2020-02-07 2022-10-18 Vecprobio Inc Vectores virales adeno-asociados recombinantes en plantas.
MY205588A (en) * 2021-02-23 2024-10-28 Univ Malaya A process for producing pharmaceutical or other heterologous protein via agrobacterium-mediated transformation of mucuna bracteata
CN114049913B (zh) * 2021-11-10 2024-04-16 桂林理工大学 植物转录因子互作影响的评估方法
CN115044611A (zh) * 2022-06-29 2022-09-13 河北农业大学 一种便于操作的烟草瞬时转化方法

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5187073A (en) 1986-06-30 1993-02-16 The University Of Toledo Process for transforming gramineae and the products thereof
US6162965A (en) 1997-06-02 2000-12-19 Novartis Ag Plant transformation methods
WO1999014348A1 (en) 1997-09-12 1999-03-25 Performance Plants, Inc. In planta transformation of plants
US5994624A (en) 1997-10-20 1999-11-30 Cotton Incorporated In planta method for the production of transgenic plants
EP1034286B1 (de) * 1997-11-18 2010-01-06 Pioneer Hi-Bred International, Inc. Mobilisierung eines viralen genoms aus t-dna durch ortsspezifische rekombinationssysteme
US6303848B1 (en) 1998-01-16 2001-10-16 Large Scale Biology Corporation Method for conferring herbicide, pest, or disease resistance in plant hosts
US6635806B1 (en) 1998-05-14 2003-10-21 Dekalb Genetics Corporation Methods and compositions for expression of transgenes in plants
US6781044B2 (en) 1998-06-25 2004-08-24 Ventria Bioscience Plant selectable marker and plant transformation method
CZ301610B6 (cs) 1999-04-19 2010-04-28 Biogemma S.A.S. Zpusob transformace rostlin
AU6780700A (en) 1999-08-18 2001-03-13 Paradigm Genetics, Inc. Methods and apparatus for transformation of monocotyledenous plants using agrobacterium in combination with vacuum filtration
US6740526B1 (en) 1999-09-22 2004-05-25 Wayne R. Curtis Quantitative transient protein expression in plant tissue culture
DK1240340T3 (da) 1999-12-16 2012-08-06 Monsanto Technology Llc Dna-konstrukter til ekspression af heterologe polypeptider i planter
US20030031648A1 (en) 2000-11-28 2003-02-13 Virogene Ltd. Vectors for expressing heterologous peptides at the amino-terminus of potyvirus coat protein, methods for use thereof, plants infected with same and methods of vaccination using same
NZ536037A (en) 2002-03-20 2008-04-30 Simplot Co J R Refined plant transformation
JP4170078B2 (ja) 2002-11-25 2008-10-22 クミアイ化学工業株式会社 アグロバクテリウム・ツメファシエンスによるケナフ植物のインプランタ形質転換法
CN1997742A (zh) * 2002-12-23 2007-07-11 阿尔特生物科学公司 植物中药用的重要蛋白质的瞬间制造方法
JP4754968B2 (ja) 2003-09-08 2011-08-24 クミアイ化学工業株式会社 植物のインプランタ形質転換法
TW200516147A (en) 2003-10-15 2005-05-16 Phytoculture Control Co Ltd Method for transforming plants and equipment for use therein
EP1616959A1 (de) 2004-07-07 2006-01-18 Icon Genetics AG Biologisch sichere transiente Proteinexpression in Pflanzen
FR2877014B1 (fr) 2004-10-21 2009-07-10 Inst Rech Pour Le Dev I R D Et Systemes d'expression de proteines recombinantes et leurs applications
WO2008036424A2 (en) 2006-09-22 2008-03-27 Regents Of The University Of California Chemically inducible cucumber mosaic virus protein expression system
CN101096682B (zh) 2007-02-06 2010-05-19 东北师范大学 植物中外源基因的瞬时表达方法

Also Published As

Publication number Publication date
EP2440035B1 (de) 2017-03-08
BR112012000302A2 (pt) 2016-09-20
PT2440035T (pt) 2017-05-22
CN102802405A (zh) 2012-11-28
MX2011013292A (es) 2012-01-12
CA2764570A1 (en) 2010-12-16
MX354857B (es) 2018-03-23
EP2440035A4 (de) 2013-03-27
CA2764570C (en) 2017-08-15
US20100319089A1 (en) 2010-12-16
US8642839B2 (en) 2014-02-04
AU2010260019A1 (en) 2011-12-22
AU2010260019B2 (en) 2014-09-11
EP2440035A1 (de) 2012-04-18
US20140123341A1 (en) 2014-05-01
US9862960B2 (en) 2018-01-09
WO2010144775A1 (en) 2010-12-16

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