MX354857B - Metodo para la expresion transitoria de acidos nucleicos en las plantas. - Google Patents

Metodo para la expresion transitoria de acidos nucleicos en las plantas.

Info

Publication number
MX354857B
MX354857B MX2014009061A MX2014009061A MX354857B MX 354857 B MX354857 B MX 354857B MX 2014009061 A MX2014009061 A MX 2014009061A MX 2014009061 A MX2014009061 A MX 2014009061A MX 354857 B MX354857 B MX 354857B
Authority
MX
Mexico
Prior art keywords
protein
evaluation
targeting
plants
plant
Prior art date
Application number
MX2014009061A
Other languages
English (en)
Inventor
Azhakanandam Kasi
Original Assignee
Syngenta Participations Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag filed Critical Syngenta Participations Ag
Publication of MX354857B publication Critical patent/MX354857B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a composiciones y métodos para la expresión transitoria de proteínas en una planta. Las composiciones comprenden plantas, semillas, tejido vegetal y partes de plantas que expresan una proteína, donde la proteína se expresa transitoriamente y la expresión transitoria de la proteína puede utilizarse como un modelo predictivo de cómo la proteína se expresará en plantas transgénicas estables en relación con datos cualitativos y cuantitativos. El modelo predictivo puede utilizarse, sin carácter taxativo, para: la evaluación de promotores, la evaluación de la construcción del casete de expresión para determinar cómo rendiría mejor (por ejemplo, adición de potenciadores o supresores del silenciamiento génico), la evaluación de las mejores formas de expresar genes hetorólogos (por ejemplo, mutación puntual, direccionamiento), la evaluación rápida de la inactivación de genes endógenos, la evaluación de los niveles de expresión proteica, el direccionamiento celular, el direccionamiento a tejidos, los potenciadores de la transcripción, la toxicidad proteica de los potenciadores de la traducción y el perfil metabólico. También se proporcionan métodos de uso.
MX2014009061A 2009-06-11 2010-06-11 Metodo para la expresion transitoria de acidos nucleicos en las plantas. MX354857B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18602509P 2009-06-11 2009-06-11
PCT/US2010/038271 WO2010144775A1 (en) 2009-06-11 2010-06-11 A method for the transient expression of nucleic acids in plants

Publications (1)

Publication Number Publication Date
MX354857B true MX354857B (es) 2018-03-23

Family

ID=43307600

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2011013292A MX2011013292A (es) 2009-06-11 2010-06-11 Metodo para la expresion transitoria de acidos nucleicos en las plantas.
MX2014009061A MX354857B (es) 2009-06-11 2010-06-11 Metodo para la expresion transitoria de acidos nucleicos en las plantas.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2011013292A MX2011013292A (es) 2009-06-11 2010-06-11 Metodo para la expresion transitoria de acidos nucleicos en las plantas.

Country Status (10)

Country Link
US (2) US8642839B2 (es)
EP (1) EP2440035B1 (es)
CN (1) CN102802405A (es)
AU (1) AU2010260019B2 (es)
BR (1) BR112012000302A2 (es)
CA (1) CA2764570C (es)
ES (1) ES2624276T3 (es)
MX (2) MX2011013292A (es)
PT (1) PT2440035T (es)
WO (1) WO2010144775A1 (es)

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WO2011084370A1 (en) * 2010-01-05 2011-07-14 Syngenta Participations Ag Constitutive synthetic plant promoters and methods of use
JP5472089B2 (ja) * 2010-12-28 2014-04-16 トヨタ自動車株式会社 光合成組織における遺伝子発現制御に関わるｄｎａ
WO2012159891A1 (en) 2011-05-20 2012-11-29 Syngenta Participations Ag Endosperm-specific plant promoters and uses therefor
AU2012284205B2 (en) 2011-07-15 2016-11-17 Syngenta Participations Ag Methods of increasing yield and stress tolerance in a plant
EP2647715A1 (en) * 2012-04-03 2013-10-09 Nomad Bioscience GmbH Agrobacterium for transient transfection of whole plants
AU2014278519B2 (en) * 2013-06-11 2020-09-10 Syngenta Participations Ag Methods for generating transgenic plants
TW201527312A (zh) * 2013-12-31 2015-07-16 Dow Agrosciences Llc 新穎玉米泛素啓動子（一）
TW201527315A (zh) * 2013-12-31 2015-07-16 Dow Agrosciences Llc 新穎玉米泛素啓動子（四）
AR101167A1 (es) * 2014-07-10 2016-11-30 Benson Hill Biosystems Inc Composiciones y métodos para incrementar el crecimiento y rendimiento de las plantas
WO2016007544A1 (en) * 2014-07-11 2016-01-14 Matatu, Inc. Use of a gut microbiome as a predictor of animal growth or health
US11926834B2 (en) * 2019-11-05 2024-03-12 Pairwise Plants Services, Inc. Compositions and methods for RNA-encoded DNA-replacement of alleles
KR20220139903A (ko) * 2020-02-07 2022-10-17 벡프로바이오, 인크. 식물에서의 재조합 아데노-관련 바이러스 벡터
WO2022182224A1 (en) * 2021-02-23 2022-09-01 Universiti Malaya A process for producing pharmaceutical or other heterologous protein via agrobacterium-mediated transformation of mucuna bracteata
CN114049913B (zh) * 2021-11-10 2024-04-16 桂林理工大学 植物转录因子互作影响的评估方法
CN115044611A (zh) * 2022-06-29 2022-09-13 河北农业大学 一种便于操作的烟草瞬时转化方法

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US5569597A (en) * 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5187073A (en) * 1986-06-30 1993-02-16 The University Of Toledo Process for transforming gramineae and the products thereof
US6162965A (en) * 1997-06-02 2000-12-19 Novartis Ag Plant transformation methods
WO1999014348A1 (en) 1997-09-12 1999-03-25 Performance Plants, Inc. In planta transformation of plants
US5994624A (en) 1997-10-20 1999-11-30 Cotton Incorporated In planta method for the production of transgenic plants
DE69841438D1 (de) * 1997-11-18 2010-02-25 Pioneer Hi Bred Int Mobilisierung eines viralen genoms aus t-dna durch ortsspezifische rekombinationssysteme
US6303848B1 (en) 1998-01-16 2001-10-16 Large Scale Biology Corporation Method for conferring herbicide, pest, or disease resistance in plant hosts
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US6781044B2 (en) * 1998-06-25 2004-08-24 Ventria Bioscience Plant selectable marker and plant transformation method
PL202067B1 (pl) 1999-04-19 2009-05-29 Biogemma S A S Sposoby transformacji roślin oraz zastosowanie Agrobacterium w sposobie transformacji
AU6780700A (en) 1999-08-18 2001-03-13 Paradigm Genetics, Inc. Methods and apparatus for transformation of monocotyledenous plants using agrobacterium in combination with vacuum filtration
US6740526B1 (en) * 1999-09-22 2004-05-25 Wayne R. Curtis Quantitative transient protein expression in plant tissue culture
PT1240340E (pt) * 1999-12-16 2012-08-01 Monsanto Technology Llc Construções de dna para expressão de polipéptidos heterólogos em plantas
US20030031648A1 (en) 2000-11-28 2003-02-13 Virogene Ltd. Vectors for expressing heterologous peptides at the amino-terminus of potyvirus coat protein, methods for use thereof, plants infected with same and methods of vaccination using same
US7598430B2 (en) 2002-03-20 2009-10-06 J.R. Simplot Company Refined plant transformation
JP4170078B2 (ja) 2002-11-25 2008-10-22 クミアイ化学工業株式会社 アグロバクテリウム・ツメファシエンスによるケナフ植物のインプランタ形質転換法
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Also Published As

Publication number Publication date
CA2764570A1 (en) 2010-12-16
EP2440035A4 (en) 2013-03-27
EP2440035B1 (en) 2017-03-08
US20100319089A1 (en) 2010-12-16
WO2010144775A1 (en) 2010-12-16
US9862960B2 (en) 2018-01-09
CA2764570C (en) 2017-08-15
CN102802405A (zh) 2012-11-28
US20140123341A1 (en) 2014-05-01
US8642839B2 (en) 2014-02-04
EP2440035A1 (en) 2012-04-18
MX2011013292A (es) 2012-01-12
AU2010260019B2 (en) 2014-09-11
BR112012000302A2 (pt) 2016-09-20
AU2010260019A1 (en) 2011-12-22
PT2440035T (pt) 2017-05-22
ES2624276T3 (es) 2017-07-13

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