MX354857B - Metodo para la expresion transitoria de acidos nucleicos en las plantas. - Google Patents
Metodo para la expresion transitoria de acidos nucleicos en las plantas.Info
- Publication number
- MX354857B MX354857B MX2014009061A MX2014009061A MX354857B MX 354857 B MX354857 B MX 354857B MX 2014009061 A MX2014009061 A MX 2014009061A MX 2014009061 A MX2014009061 A MX 2014009061A MX 354857 B MX354857 B MX 354857B
- Authority
- MX
- Mexico
- Prior art keywords
- protein
- evaluation
- targeting
- plants
- plant
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente invención se refiere a composiciones y métodos para la expresión transitoria de proteínas en una planta. Las composiciones comprenden plantas, semillas, tejido vegetal y partes de plantas que expresan una proteína, donde la proteína se expresa transitoriamente y la expresión transitoria de la proteína puede utilizarse como un modelo predictivo de cómo la proteína se expresará en plantas transgénicas estables en relación con datos cualitativos y cuantitativos. El modelo predictivo puede utilizarse, sin carácter taxativo, para: la evaluación de promotores, la evaluación de la construcción del casete de expresión para determinar cómo rendiría mejor (por ejemplo, adición de potenciadores o supresores del silenciamiento génico), la evaluación de las mejores formas de expresar genes hetorólogos (por ejemplo, mutación puntual, direccionamiento), la evaluación rápida de la inactivación de genes endógenos, la evaluación de los niveles de expresión proteica, el direccionamiento celular, el direccionamiento a tejidos, los potenciadores de la transcripción, la toxicidad proteica de los potenciadores de la traducción y el perfil metabólico. También se proporcionan métodos de uso.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US18602509P | 2009-06-11 | 2009-06-11 | |
PCT/US2010/038271 WO2010144775A1 (en) | 2009-06-11 | 2010-06-11 | A method for the transient expression of nucleic acids in plants |
Publications (1)
Publication Number | Publication Date |
---|---|
MX354857B true MX354857B (es) | 2018-03-23 |
Family
ID=43307600
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2011013292A MX2011013292A (es) | 2009-06-11 | 2010-06-11 | Metodo para la expresion transitoria de acidos nucleicos en las plantas. |
MX2014009061A MX354857B (es) | 2009-06-11 | 2010-06-11 | Metodo para la expresion transitoria de acidos nucleicos en las plantas. |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2011013292A MX2011013292A (es) | 2009-06-11 | 2010-06-11 | Metodo para la expresion transitoria de acidos nucleicos en las plantas. |
Country Status (10)
Country | Link |
---|---|
US (2) | US8642839B2 (es) |
EP (1) | EP2440035B1 (es) |
CN (1) | CN102802405A (es) |
AU (1) | AU2010260019B2 (es) |
BR (1) | BR112012000302A2 (es) |
CA (1) | CA2764570C (es) |
ES (1) | ES2624276T3 (es) |
MX (2) | MX2011013292A (es) |
PT (1) | PT2440035T (es) |
WO (1) | WO2010144775A1 (es) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102724866B (zh) * | 2010-01-05 | 2015-04-15 | 先正达参股股份有限公司 | 组成型合成植物启动子以及使用方法 |
JP5472089B2 (ja) * | 2010-12-28 | 2014-04-16 | トヨタ自動車株式会社 | 光合成組織における遺伝子発現制御に関わるdna |
WO2012159891A1 (en) | 2011-05-20 | 2012-11-29 | Syngenta Participations Ag | Endosperm-specific plant promoters and uses therefor |
MX347810B (es) | 2011-07-15 | 2017-05-15 | Syngenta Participations Ag | Métodos para mejorar el rendimiento y la tolerancia al estrés en una planta. |
EP2647715A1 (en) * | 2012-04-03 | 2013-10-09 | Nomad Bioscience GmbH | Agrobacterium for transient transfection of whole plants |
US10443063B2 (en) * | 2013-06-11 | 2019-10-15 | Syngenta Participations Ag | Methods for generating transgenic plants |
TW201527312A (zh) * | 2013-12-31 | 2015-07-16 | Dow Agrosciences Llc | 新穎玉米泛素啓動子(一) |
TW201527315A (zh) * | 2013-12-31 | 2015-07-16 | Dow Agrosciences Llc | 新穎玉米泛素啓動子(四) |
BR112017000376A2 (pt) * | 2014-07-10 | 2017-11-07 | Benson Hill Biosystems Inc | composições e métodos para aumentar o desenvolvimento vegetal e seu rendimento |
WO2016007544A1 (en) * | 2014-07-11 | 2016-01-14 | Matatu, Inc. | Use of a gut microbiome as a predictor of animal growth or health |
AU2020380319A1 (en) * | 2019-11-05 | 2022-05-12 | Pairwise Plants Services, Inc. | Compositions and methods for RNA-encoded DNA-replacement of alleles |
WO2021158648A1 (en) * | 2020-02-07 | 2021-08-12 | Vecprobio, Inc. | Recombinant adeno-associated viral vectors in plants |
WO2022182224A1 (en) * | 2021-02-23 | 2022-09-01 | Universiti Malaya | A process for producing pharmaceutical or other heterologous protein via agrobacterium-mediated transformation of mucuna bracteata |
CN114049913B (zh) * | 2021-11-10 | 2024-04-16 | 桂林理工大学 | 植物转录因子互作影响的评估方法 |
CN115044611A (zh) * | 2022-06-29 | 2022-09-13 | 河北农业大学 | 一种便于操作的烟草瞬时转化方法 |
Family Cites Families (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5569597A (en) | 1985-05-13 | 1996-10-29 | Ciba Geigy Corp. | Methods of inserting viral DNA into plant material |
US5187073A (en) | 1986-06-30 | 1993-02-16 | The University Of Toledo | Process for transforming gramineae and the products thereof |
US6162965A (en) | 1997-06-02 | 2000-12-19 | Novartis Ag | Plant transformation methods |
ZA988332B (en) | 1997-09-12 | 1999-03-23 | Performance Plants Inc | In planta transformation of plants |
US5994624A (en) | 1997-10-20 | 1999-11-30 | Cotton Incorporated | In planta method for the production of transgenic plants |
ATE454459T1 (de) * | 1997-11-18 | 2010-01-15 | Pioneer Hi Bred Int | Mobilisierung eines viralen genoms aus t-dna durch ortsspezifische rekombinationssysteme |
US6303848B1 (en) | 1998-01-16 | 2001-10-16 | Large Scale Biology Corporation | Method for conferring herbicide, pest, or disease resistance in plant hosts |
US6635806B1 (en) | 1998-05-14 | 2003-10-21 | Dekalb Genetics Corporation | Methods and compositions for expression of transgenes in plants |
US6781044B2 (en) | 1998-06-25 | 2004-08-24 | Ventria Bioscience | Plant selectable marker and plant transformation method |
AR023576A1 (es) | 1999-04-19 | 2002-09-04 | Biogemma Fr | Metodo para la transformacion de una planta objetivo, uso de agrobacterium para dicho metodo y metodo para producir un tejido de planta transformada. |
AU6780700A (en) | 1999-08-18 | 2001-03-13 | Paradigm Genetics, Inc. | Methods and apparatus for transformation of monocotyledenous plants using agrobacterium in combination with vacuum filtration |
US6740526B1 (en) | 1999-09-22 | 2004-05-25 | Wayne R. Curtis | Quantitative transient protein expression in plant tissue culture |
BRPI0017613B1 (pt) | 1999-12-16 | 2017-04-04 | Monsanto Technology Llc | promotor híbrido, construto de dna, bem como métodos para produção de plantas transgênicas, para expressar uma sequência de dna estrutural em uma planta e para controlar ervas daninhas |
US20030031648A1 (en) | 2000-11-28 | 2003-02-13 | Virogene Ltd. | Vectors for expressing heterologous peptides at the amino-terminus of potyvirus coat protein, methods for use thereof, plants infected with same and methods of vaccination using same |
CA2479739C (en) | 2002-03-20 | 2013-02-19 | J.R. Simplot Company | Refined plant transformation |
JP4170078B2 (ja) | 2002-11-25 | 2008-10-22 | クミアイ化学工業株式会社 | アグロバクテリウム・ツメファシエンスによるケナフ植物のインプランタ形質転換法 |
EP1596803A4 (en) * | 2002-12-23 | 2007-12-12 | Altor Bioscience Corp | TRANSIENT PRODUCTION OF PHARMACEUTICALLY IMPORTANT PROTEINS IN PLANTS |
JP4754968B2 (ja) | 2003-09-08 | 2011-08-24 | クミアイ化学工業株式会社 | 植物のインプランタ形質転換法 |
TW200516147A (en) | 2003-10-15 | 2005-05-16 | Phytoculture Control Co Ltd | Method for transforming plants and equipment for use therein |
EP1616959A1 (en) | 2004-07-07 | 2006-01-18 | Icon Genetics AG | Biological safe transient protein expression in plants |
FR2877014B1 (fr) | 2004-10-21 | 2009-07-10 | Inst Rech Pour Le Dev I R D Et | Systemes d'expression de proteines recombinantes et leurs applications |
WO2008036424A2 (en) | 2006-09-22 | 2008-03-27 | Regents Of The University Of California | Chemically inducible cucumber mosaic virus protein expression system |
CN101096682B (zh) | 2007-02-06 | 2010-05-19 | 东北师范大学 | 植物中外源基因的瞬时表达方法 |
-
2010
- 2010-06-11 EP EP10786895.2A patent/EP2440035B1/en active Active
- 2010-06-11 CN CN2010800292090A patent/CN102802405A/zh active Pending
- 2010-06-11 PT PT107868952T patent/PT2440035T/pt unknown
- 2010-06-11 BR BR112012000302A patent/BR112012000302A2/pt not_active IP Right Cessation
- 2010-06-11 WO PCT/US2010/038271 patent/WO2010144775A1/en active Application Filing
- 2010-06-11 ES ES10786895.2T patent/ES2624276T3/es active Active
- 2010-06-11 CA CA2764570A patent/CA2764570C/en active Active
- 2010-06-11 MX MX2011013292A patent/MX2011013292A/es active IP Right Grant
- 2010-06-11 US US12/813,681 patent/US8642839B2/en active Active
- 2010-06-11 AU AU2010260019A patent/AU2010260019B2/en active Active
- 2010-06-11 MX MX2014009061A patent/MX354857B/es unknown
-
2013
- 2013-12-23 US US14/138,443 patent/US9862960B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
BR112012000302A2 (pt) | 2016-09-20 |
AU2010260019B2 (en) | 2014-09-11 |
US9862960B2 (en) | 2018-01-09 |
CA2764570A1 (en) | 2010-12-16 |
EP2440035A4 (en) | 2013-03-27 |
PT2440035T (pt) | 2017-05-22 |
EP2440035A1 (en) | 2012-04-18 |
CA2764570C (en) | 2017-08-15 |
US20140123341A1 (en) | 2014-05-01 |
US20100319089A1 (en) | 2010-12-16 |
US8642839B2 (en) | 2014-02-04 |
MX2011013292A (es) | 2012-01-12 |
EP2440035B1 (en) | 2017-03-08 |
AU2010260019A1 (en) | 2011-12-22 |
ES2624276T3 (es) | 2017-07-13 |
CN102802405A (zh) | 2012-11-28 |
WO2010144775A1 (en) | 2010-12-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
MX354857B (es) | Metodo para la expresion transitoria de acidos nucleicos en las plantas. | |
EA201201264A1 (ru) | Молекулы полинуклеотидов для регуляции генов у растений | |
WO2012158986A3 (en) | Methods for site-specific genetic modification in stem cells using xanthomonas tal nucleases (xtn) for the creation of model organisms | |
TR201806812T4 (tr) | Rna-yönlendirmeli hedef dna modifikasyonu için ve rna-yönlendirmeli transkripsiyon modifikasyonu için yöntemler ve bileşimler. | |
AR092481A1 (es) | Desempeño de ubicacion de fad2 y de las proteinas especificas correspondientes de union de ubicacion selectiva capaces de inducir rupturas selectivas | |
NZ591126A (en) | INCREASED HETEROLOGOUS Fe-S ENZYME ACTIIVTY IN YEAST | |
WO2014022702A3 (en) | Methods and compositions for controlling gene expression by rna processing | |
EA201401067A1 (ru) | Синтетические соединения для вегетативной реакции на ава | |
IN2014CN02754A (es) | ||
MX346923B (es) | Genes y proteinas para sintesis de alcanoil-coa. | |
MX2011011945A (es) | Promotor vegetal operable en la capa de transferencia endospermica basal y usos del mismo. | |
DK2285969T3 (da) | Anvendelse af proteinkinase b og valproinsyre for at forøge heterolog genekspression i pattedyrsceller | |
HK1256678A1 (zh) | 用干擾 rna 的凋亡細胞介導的哺乳動物細胞轉染 | |
BRPI0516039A (pt) | moléculas promotoras para o uso em plantas | |
DE602005019165D1 (de) | NACHWEIS VON ZELLULÄREM STRESS mittels Hmox-1 Promoter | |
UA113613C2 (xx) | СПОСІБ ВИЗНАЧЕННЯ ЗИГОТНОСТІ bm3 МУТАНТНОГО АЛЕЛЯ І АЛЕЛЯ COMT ДИКОГО ТИПУ З ВИКОРИСТАННЯМ ТКАНИНИ РОСЛИНИ КУКУРУДЗИ | |
RU2013123512A (ru) | Муриновая модель воспаления с делецией il33 n-концевого домена | |
EA201391666A1 (ru) | Рекомбинантная экспрессия растворимого интерферона | |
WO2008064056A3 (en) | Tetracycline repressor and uses thereof | |
WO2011008510A3 (en) | Plant seeds with altered storage compound levels, related constructs and methods involving genes encoding cytosolic pyrophosphatase | |
WO2018067608A3 (en) | Animal model of angelman syndrome | |
BR112013024346A2 (pt) | técnica de interferência de tra-2 rna do mosquito culicinae para geneticamente produzir populações de machos | |
UA70134U (ru) | Способ приготовления силоса для кормления крупного рогатого скота | |
Xiaoying et al. | Analysis of seed-specificity of RNAi silencing the fad2 gene expression of transgenic rapeseed (Brassica napus) | |
Boydens et al. | Embedded software development driven by tests |