ES2582169T3 - Análisis de expresión génica con oligonucleótidos etiquetados con elementos - Google Patents

Análisis de expresión génica con oligonucleótidos etiquetados con elementos Download PDF

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Publication number
ES2582169T3
ES2582169T3 ES07710634.2T ES07710634T ES2582169T3 ES 2582169 T3 ES2582169 T3 ES 2582169T3 ES 07710634 T ES07710634 T ES 07710634T ES 2582169 T3 ES2582169 T3 ES 2582169T3
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cell
probe
tag
isolated
nucleic acid
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Olga Ornatsky
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Standard Biotools Canada Inc
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Fluidigm Canada Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/0027—Methods for using particle spectrometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material

Abstract

Un método para el análisis celular en una célula o partícula celular, en donde dicha célula es una célula entera de origen animal, vegetal, bacteriano o fúngico, o dicha partícula celular se selecciona del grupo que consiste en un cromosoma aislado, un núcleo aislado, una mitocondria aislada, un cloroplasto aislado, y un virus aislado, comprendiendo el método: (a) fijar y permeabilizar la célula o la partícula celular; (b) incubar la célula o la partícula celular en una solución de hibridación con una sonda de ácido nucleico específica para un ácido nucleico diana, donde la sonda marcada con una etiqueta elemental única tal que un tipo de dicha sonda marcada con un tipo de dicha etiqueta es distinguible de cualquier otro tipo de dicha sonda marcada con un tipo diferente de dicha etiqueta por análisis elemental ICP-MS; (c) separar la sonda no hibridada de la sonda hibridada al ácido nucleico diana mediante estrictas condiciones de lavado; y (d) analizar la célula o partícula celular mediante ICP-MS para identificar la sonda y cuantificar la sonda unida al ácido nucleico diana, en donde cada elemento marcado comprende un resto químico que incluye un átomo elemental o una multitud de átomos elementales con uno o muchos isótopos unidos a una estructura molecular de soporte, y en donde la etiqueta elemental comprende además unos medios de unión de dicha etiqueta a un sustrato.

Description

imagen1
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(en el plato o placa de pocillo) podría indicar la identidad de la sonda complementaria que está unida a ella. Las instrucciones serán similares a los apartados de (i) a (v) descritos anteriormente, pero en este caso sólo se mide el elemento unido al oligo(dT)n
Los kits descritos anteriormente pueden comprender además reactivos y dispositivos que se seleccionan del grupo
5 que consiste en soluciones de disociación, columnas giratorias con membranas de unión a ácido nucleico, columna de purificación para aislamiento y purificación de ácidos nucleicos de muestras biológicas, reactivos y soluciones para amplificación de ácidos nucleicos purificados, patrones, tampón diluyente, tampón de disociación, tampón de lavado, tampón de hibridación y tampón de ensayo. Los ácidos nucleicos endógenos se pueden amplificar in situ en células morfológicamente intactas. El elemento se puede medir utilizando un espectrómetro de masas. El elemento
10 puede ser un isótopo o ión. El elemento se puede seleccionar del grupo que consiste en elementos de transición, metales nobles, lantánidos, elementos de tierras raras, oro, plata, platino, rodio, iridio y paladio. El elemento puede incluir más de un elemento y/o más de un isótopo y/o más de un átomo de un isótopo. Los productos de afinidad se pueden seleccionar del grupo que consiste en un anticuerpo, Fab, aptámero, antígeno, hormona, factor de crecimiento, receptor, proteína y ácido nucleico. Los kits también pueden incluir la instrucción para el análisis
15 elemental de partículas.
Los kits pueden comprender los siguientes componentes:
(a)
reactivos para amplificación in situ
(b)
reactivos y dispositivos para la purificación de ácido nucleico
(c)
tampón de hibridación in situ 20 (d) solución de fijación y permeabilización
(e)
solución de lavado
(f)
reactivo de disolución Las enseñanzas del solicitante proporcionan los métodos divulgados anteriormente. Los métodos permiten:
(a)
la multiplexación25 (b) el análisis simultáneo de la expresión génica y proteica
(c)
métodos con o sin etapas de amplificación
(d)
análisis de bajo coste sin enzimas polimerasa caras
(e)
el análisis genético en una sola célula
(f)
la cuantificación absoluta de la expresión génica
30
13
13
imagen12
19. Janicki, S. M.; Tsukamoto, T.; Salghetti, S. E.; Tansey, W. P.; Sachidanandam, R.; Prasanth, K. V.; Ried, T.; Shav-Tal, Y.; Bertrand, E.; Singer, R. H.; Spector, D. L. From silencing to gene expression: Real-time analysis in single cells Cell 2004, 116, 683-98.
20. Weier, H. U.; Chu, L. W.; Murnane, J. P.; Weier, J. F. Applications and technical challenges of fluorescence in 5 situ hybridization in stem cell research Blood Cells Mol.Dis. 2004, 32, 68-76.
21. Derradji, H.; Bekaert, S.; Van Oostveldt, P.; Baatout, S. Comparison of different protocols for telomere length estimation by combination of quantitative fluorescence in situ hybridization (Q-FISH) and flow cytometry in human cancer cell lines Anticancer Res. 2005, 25, 1039-50.
22. Baranov, V. I.; Quinn, Z.; Bandura, D. R.; Tanner, S. D. A sensitive and quantitative element-tagged 10 immunoassay with ICPMS detection Analytical Chemistry 2002, 74, 1629-36. [WO2005/003767 A2]
23.
Zhang, Q. Y.; Garner, K.; Viswanatha, D. S. Rapid detection of leukemia-associated translocation fusion genes using a novel combined RT-PCR and flow cytometric method Leukemia 2002, 16, 144-49.
24.
Stein, C. A.; Subasinghe, C.; Shinozuka, K.; Cohen, J. S. Physicochemical Properties of Phosphorothioate Oligodeoxynucleotides Nucleic Acids Research 1988, 16, 3209-21.
15 25. Sambrook et al., in Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989).
26. Ausubel et al., in Current Protocols in Molecular Biology , Greene Publishing Associates and Wiley-Intersciences (1987).
15
15

Claims (1)

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ES07710634.2T 2006-02-13 2007-02-13 Análisis de expresión génica con oligonucleótidos etiquetados con elementos Active ES2582169T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US77258806P 2006-02-13 2006-02-13
US772588P 2006-02-13
PCT/CA2007/000223 WO2007093050A1 (en) 2006-02-13 2007-02-13 Gene expression assays conducted by elemental analysis

Publications (1)

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ES2582169T3 true ES2582169T3 (es) 2016-09-09

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US (3) US20070190560A1 (es)
EP (3) EP1991694B1 (es)
JP (2) JP5777269B2 (es)
CN (1) CN101384733A (es)
CA (1) CA2640508C (es)
DK (1) DK1991694T3 (es)
ES (1) ES2582169T3 (es)
WO (1) WO2007093050A1 (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7479630B2 (en) * 2004-03-25 2009-01-20 Bandura Dmitry R Method and apparatus for flow cytometry linked with elemental analysis
DK1991694T3 (da) * 2006-02-13 2016-08-01 Fluidigm Canada Inc Genekspressionsanalyse med grundstoftagget oligonukleotid
US8101368B2 (en) * 2006-02-13 2012-01-24 Dvs Sciences Inc. Quantitation of cellular DNA and cell numbers using element labeling
US8283624B2 (en) 2006-08-15 2012-10-09 Dvs Sciences Inc. Apparatus and method for elemental analysis of particles by mass spectrometry
EP2982963B1 (en) * 2006-11-02 2019-08-21 Fluidigm Canada Inc. Particles containing detectable elemental code
EP2432897B1 (en) * 2009-05-20 2013-04-17 AdvanDx, Inc. Methods for whole-cell analysis of gram-positive bacteria
DE102010032075A1 (de) * 2010-07-23 2012-01-26 Eads Deutschland Gmbh Wasserstofferzeugung mittels hydrierten Polysilanen zum Betrieb von Brennstoffzellen
CN102621129A (zh) * 2012-04-18 2012-08-01 上海市毛麻纺织科学技术研究所 一种银纤维纺织品中银的定性与定量检测方法
EP4075478A1 (en) * 2012-10-26 2022-10-19 Fluidigm Canada Inc. Sample and method of sample preparation
EP2929050A1 (en) * 2012-12-10 2015-10-14 AdvanDx, Inc. Use of probes for mass spectrometric identification of microorganisms or cells and associated conditions of interest
US9218949B2 (en) 2013-06-04 2015-12-22 Fluidigm Canada, Inc. Strategic dynamic range control for time-of-flight mass spectrometry
CN103439501B (zh) * 2013-08-23 2016-03-23 河南省科学院高新技术研究中心 一种用电感耦合等离子体质谱法检测乙肝表面抗原的方法
AU2014318698B2 (en) * 2013-09-13 2019-10-24 The Board Of Trustees Of The Leland Stanford Junior University Multiplexed imaging of tissues using mass tags and secondary ion mass spectrometry
EP3325493A4 (en) * 2015-06-29 2019-03-13 Fluidigm Canada Inc. SYSTEMS, METHODS, AND COMPOSITIONS FOR SIMULTANEOUS DETECTION OF RNA AND PROTEIN BY MEANS OF MASS SPECTROMETRY
JPWO2018008435A1 (ja) * 2016-07-04 2019-04-18 株式会社ダナフォーム 核酸分析方法
US11187707B2 (en) * 2019-01-30 2021-11-30 Regeneron Pharmaceuticals, Inc. Method of characterization of visible and/or sub-visible particles in biologics
US20230151355A1 (en) * 2019-03-12 2023-05-18 Universal Sequencing Technology Corporation Methods for Single Cell Intracellular Capture and its Applications
CN110487923B (zh) * 2019-03-22 2023-04-07 中国计量科学研究院 一种基于分子排阻色谱-电感耦合等离子体质谱联用的线性质粒dna含量的检测方法
US20220145376A1 (en) * 2019-05-17 2022-05-12 Shimadzu Corporation Method for detecting extracelluar vesicles
CN111575383A (zh) * 2020-06-10 2020-08-25 四川省疾病预防控制中心 基于金属纳米探针的多种宫颈癌miRNA标志物同时检测的方法
CN113355394A (zh) * 2021-06-15 2021-09-07 杭州谱育科技发展有限公司 基于质谱技术的核酸检测方法
CN113355393A (zh) * 2021-06-15 2021-09-07 杭州谱育科技发展有限公司 基于金属编码技术的核酸检测方法

Family Cites Families (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4022876A (en) * 1973-06-21 1977-05-10 Stanford Research Institute Mass spectrometric immunoassay
IL49685A (en) * 1976-05-31 1978-10-31 Technion Res & Dev Foundation Specific binding assay method for determining the concentration of materials and reagent means therefor
JPS607166A (ja) 1983-06-24 1985-01-14 Toshiba Corp 保護回路
ZA862334B (en) 1985-03-28 1987-11-25 Cetus Corp Process for amplifying,detecting and/or cloning nucleic acid sequences
DK171161B1 (da) 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5079172A (en) * 1988-11-04 1992-01-07 Board Of Trustees Operating Michigan State University Method for detecting the presence of antibodies using gold-labeled antibodies and test kit
DK130991D0 (da) * 1991-07-04 1991-07-04 Immunodex K S Polymere konjugater
US6391590B1 (en) * 1991-10-21 2002-05-21 The Regents Of The University Of California Recombinant streptavidin-metallothionein chimeric protein having biological recognition specificity
US5569754A (en) * 1993-06-11 1996-10-29 Board Of Regents, University Of Tx Systems RNA import elements for transport into mitochondria
GB9315847D0 (en) * 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US5773823A (en) * 1993-09-10 1998-06-30 Seiko Instruments Inc. Plasma ion source mass spectrometer
JP3188794B2 (ja) * 1993-09-10 2001-07-16 セイコーインスツルメンツ株式会社 プラズマイオン源質量分析装置
DE69535428T2 (de) 1994-02-14 2007-12-06 Smithkline Beecham Corp. Verfahren zum Auffinden von differentiel exprimierte Gene
US5521289A (en) * 1994-07-29 1996-05-28 Nanoprobes, Inc. Small organometallic probes
WO1997027317A1 (en) 1996-01-23 1997-07-31 Affymetrix, Inc. Nucleic acid analysis techniques
US5981180A (en) * 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
ATE496288T1 (de) * 1995-10-11 2011-02-15 Luminex Corp Gleichzeitige mehrfachanalyse klinischer proben
JPH09115476A (ja) * 1995-10-19 1997-05-02 Seiko Instr Inc プラズマイオン質量分析装置
EP0880598A4 (en) 1996-01-23 2005-02-23 Affymetrix Inc RAPID EVALUATION OF NUCLEIC ACID ABUNDANCE DIFFERENCE, WITH A HIGH-DENSITY OLIGONUCLEOTIDE SYSTEM
US6116516A (en) * 1996-05-13 2000-09-12 Universidad De Sevilla Stabilized capillary microjet and devices and methods for producing same
ES2140998B1 (es) * 1996-05-13 2000-10-16 Univ Sevilla Procedimiento de atomizacion de liquidos.
JP3492081B2 (ja) * 1996-05-15 2004-02-03 セイコーインスツルメンツ株式会社 プラズマイオン源質量分析装置
US6444650B1 (en) * 1996-10-01 2002-09-03 Geron Corporation Antisense compositions for detecting and inhibiting telomerase reverse transcriptase
US6449562B1 (en) * 1996-10-10 2002-09-10 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
WO1998017992A2 (en) * 1996-10-25 1998-04-30 Applied Imaging, Inc. Multifluor-fluorescence in situ hybridization (m-fish) imaging techniques using multiple multiband filters with image registration
AU735355B2 (en) * 1997-04-15 2001-07-05 Daiichi Sankyo Company, Limited Novel protein and method for producing the protein
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
CA2319170A1 (en) * 1998-01-27 1999-07-29 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
US7060457B2 (en) * 1998-03-18 2006-06-13 Johns Hopkins University Aberrant glutamate transporters and methods of use
US6290839B1 (en) * 1998-06-23 2001-09-18 Clinical Micro Sensors, Inc. Systems for electrophoretic transport and detection of analytes
US6913884B2 (en) * 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
AU2001279222B2 (en) * 2000-08-08 2006-03-02 Monogram Biosciences, Inc. Multiplexed enzymatic assays
WO2002066952A2 (en) * 2000-10-19 2002-08-29 Target Discovery, Inc Mass defect labeling for the determination of oligomer sequences
US7135296B2 (en) * 2000-12-28 2006-11-14 Mds Inc. Elemental analysis of tagged biologically active materials
CA2431864C (en) * 2000-12-28 2011-09-06 Mds Inc., Doing Business As Mds Sciex Elemental analysis of tagged biologically active materials
US7700295B2 (en) * 2000-12-28 2010-04-20 Mds Sciex Elemental analysis of tagged biologically active materials
DE10106370B4 (de) * 2001-02-12 2005-01-27 Vermicon Ag Oligonukleotidsonden zur Detektion von parodontopathogenen Bakterien mittels in situ-Hybridisierung
US7479630B2 (en) * 2004-03-25 2009-01-20 Bandura Dmitry R Method and apparatus for flow cytometry linked with elemental analysis
DE10152821B4 (de) * 2001-10-25 2006-11-16 Bruker Daltonik Gmbh Massenspektren ohne elektronisches Rauschen
US20030092090A1 (en) * 2001-11-14 2003-05-15 Kiamars Hajizadeh Rapid prion-detection device, system, and test kit
DE10227599B4 (de) * 2002-06-20 2005-04-28 Proteome Factory Ag Verfahren und Reagenz zur spezifischen Identifizierung und Quantifizierung von einem oder mehreren Proteinen in einer Probe
FI20021325A0 (fi) * 2002-07-05 2002-07-05 Valtion Teknillinen Menetelmä ja reagenssipakkaus yksittäisten polynukleotidien määrän määrittämiseksi
EP1540344A4 (en) * 2002-08-02 2007-02-21 Applera Corp FLUORESCENCE POLARIZATION ASSAY
WO2004034018A2 (en) * 2002-10-07 2004-04-22 Trellis Bioscience, Inc. Multihued labels
JP2007524347A (ja) * 2003-02-27 2007-08-30 ナノスフェアー　インコーポレイテッド マイクロアレイ形式のアッセイにおける、万能ナノ粒子プローブを用いた標識不要の遺伝子発現プロファイリング
AU2004226369A1 (en) * 2003-03-28 2004-10-14 Medimmune, Llc Compositions and methods involving respiratory syncytial virus subgroup B strain 9320
CN1580283A (zh) 2003-08-13 2005-02-16 清华大学 一种检测核酸分子的方法
WO2005095942A1 (ja) 2004-03-30 2005-10-13 Riken レーザーアブレーションを用いた生体試料の分析方法およびその装置
US7422855B2 (en) * 2004-06-10 2008-09-09 Perkinelmer Las, Inc. Multiplexing assays for analyte detection
JP5020818B2 (ja) * 2004-07-01 2012-09-05 ジェン−プローブ・インコーポレーテッド 生物学的試料中の核酸を検出するための方法及び組成物
US7462720B2 (en) * 2004-09-02 2008-12-09 Agency Science Tech & Res Determination of nucleic acid using electrocatalytic intercalators
US20060177850A1 (en) * 2004-12-30 2006-08-10 Mack Schermer Particle-based multiplex assay system with three or more assay reporters
GB2425178A (en) 2005-04-13 2006-10-18 Univ Sheffield Analysis of biological samples by mass spectrometry
US7507588B2 (en) * 2005-04-20 2009-03-24 Becton, Dickinson And Company Multiplex microparticle system
US20060240444A1 (en) * 2005-04-21 2006-10-26 Peiguo Chu Detection methods
WO2007022074A2 (en) * 2005-08-11 2007-02-22 Perkinelmer Las, Inc. Assay particles and methods of use
JP2007146149A (ja) * 2005-11-02 2007-06-14 Fujifilm Corp 蛍光性重合体微粒子、蛍光性重合体微粒子の製造方法、蛍光検出キット及び蛍光検出方法
DE102006001793A1 (de) 2006-01-12 2007-07-26 Forschungsgesellschaft für Arbeitsphysiologie und Arbeitsschutz e.V. Verfahren und diagnostisches Kit zur simultanen Identifizierung und Quantifizierung von einzelnen oder in Gemischen vorliegenden Biomolekülen und deren Modifikationen
WO2007140571A1 (en) 2006-02-13 2007-12-13 Olga Ornatsky Quantitation of cellular dna and cell numbers using element labeling
CA2642201C (en) 2006-02-13 2014-09-02 Olga Ornatsky Kinase and phosphatase assays conducted by elemental analysis
DK1991694T3 (da) 2006-02-13 2016-08-01 Fluidigm Canada Inc Genekspressionsanalyse med grundstoftagget oligonukleotid
EP2021793B1 (en) 2006-05-27 2017-04-26 Fluidigm Canada Inc. Polymer backbone element tags
CA2673912A1 (en) 2006-12-29 2008-07-10 Scott D. Tanner Cell injector for flow cytometer having mass spectrometer detector and method for using same

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