ES2562789T3 - Supresión de la amplificación usando un oligonucleótido y una polimerasa que carece significativamente de actividad exonucleasa 5'-3' - Google Patents
Supresión de la amplificación usando un oligonucleótido y una polimerasa que carece significativamente de actividad exonucleasa 5'-3' Download PDFInfo
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- ES2562789T3 ES2562789T3 ES08785392.5T ES08785392T ES2562789T3 ES 2562789 T3 ES2562789 T3 ES 2562789T3 ES 08785392 T ES08785392 T ES 08785392T ES 2562789 T3 ES2562789 T3 ES 2562789T3
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- Prior art keywords
- sequence
- blocking oligonucleotide
- oligonucleotide
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- target sequence
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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| WO2011112534A1 (en) * | 2010-03-08 | 2011-09-15 | Dana-Farber Cancer Institute, Inc. | Full cold-pcr enrichment with reference blocking sequence |
| US9944979B2 (en) * | 2010-09-10 | 2018-04-17 | Lsi Medience Corporation | Inhibition method of nucleic acid amplification by photoirradiation and method of selective nucleic acid amplification with high sensitivity |
| DK2633071T3 (en) | 2010-10-27 | 2017-01-30 | Harvard College | COMPOSITIONS OF "MAINTENANCE" PRIMER DUPLEXES AND METHODS OF USE |
| US20120225421A1 (en) * | 2011-02-28 | 2012-09-06 | Transgenomic, Inc. | Kit and method for sequencing a target dna in a mixed population |
| US11130992B2 (en) | 2011-03-31 | 2021-09-28 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable multiplex COLD-PCR |
| WO2012151328A2 (en) | 2011-05-02 | 2012-11-08 | President And Fellows Of Harvard College | Spatial sequestration of dynamic nucleic acid circuits |
| KR20210056458A (ko) | 2011-05-04 | 2021-05-18 | 바이오셉트 인코포레이티드 | 핵산 서열 변이체를 검출하는 방법 |
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| EP2758458A4 (en) | 2011-07-10 | 2015-10-21 | Harvard College | COMPOSITIONS AND METHODS FOR SELF-ASSEMBLING POLYMERS WITH COMPLEMENTARY MACROSCOPIC AND MICROSCOPIC SCALE UNITS |
| AU2012307282B2 (en) | 2011-09-16 | 2018-03-15 | Lexogen Gmbh | Nucleic acid transcription method |
| EP2570487A1 (en) | 2011-09-16 | 2013-03-20 | Lexogen GmbH | Nucleic acid transcription method |
| US9260714B2 (en) * | 2011-12-02 | 2016-02-16 | Roche Molecular Systems, Inc. | Suppression of non-specific amplification with high-homology oligonucleotides |
| US9133490B2 (en) | 2012-05-16 | 2015-09-15 | Transgenomic, Inc. | Step-up method for COLD-PCR enrichment |
| WO2014177540A1 (en) * | 2013-04-29 | 2014-11-06 | Qiagen Marseille | A method for dna amplification with a blocking oligonucleotide |
| HK1222209A1 (zh) * | 2013-10-19 | 2017-06-23 | Trovagene, Inc. | 隨時間檢測疾病中的突變 |
| JP2017515484A (ja) * | 2014-05-19 | 2017-06-15 | ウィリアム マーシュ ライス ユニバーシティＷｉｌｌｉａｍ Ｍａｒｓｈ Ｒｉｃｅ Ｕｎｉｖｅｒｓｉｔｙ | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| US12391985B2 (en) | 2014-08-07 | 2025-08-19 | Pharmassist Ltd | Method of determining PIK3CA mutational status in a sample |
| JP6770957B2 (ja) * | 2014-08-07 | 2020-10-21 | ファーマシスト リミテッドＰｈａｒｍａｓｓｉｓｔ Ｌｔｄ | 試料中のｐｉｋ３ｃａ突然変異状態を決定する方法 |
| WO2016048833A2 (en) | 2014-09-26 | 2016-03-31 | Janssen Pharmaceutica Nv | Use of fgfr mutant gene panels in identifying cancer patients that will be responsive to treatment with an fgfr inhibitor |
| US9909169B2 (en) | 2014-12-17 | 2018-03-06 | Roche Molecular Systems, Inc. | Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression |
| JP5813263B1 (ja) * | 2015-03-31 | 2015-11-17 | 日鉄住金環境株式会社 | 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド |
| US9657353B2 (en) | 2015-06-26 | 2017-05-23 | Great Basin Scientific, Inc. | Specific detection of organisms derived from a sample |
| CN109563506A (zh) | 2016-05-06 | 2019-04-02 | 威廉马歇莱思大学 | 使用随机捕获探针文库的化学计量核酸纯化 |
| JP7106461B2 (ja) * | 2016-06-07 | 2022-07-26 | セファイド | 熱安定性ポリメラーゼ阻害剤組成物及び方法 |
| US11371090B2 (en) | 2016-12-12 | 2022-06-28 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection |
| US11174511B2 (en) | 2017-07-24 | 2021-11-16 | Dana-Farber Cancer Institute, Inc. | Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture |
| WO2019099420A1 (en) * | 2017-11-15 | 2019-05-23 | Yan Wang | A method for detecting multiple dna mutations and copy number variations |
| EP3759118A4 (en) | 2018-02-20 | 2022-03-23 | William Marsh Rice University | Systems and methods for allele enrichment using multiplexed blocker displacement amplification |
| ES2874753T3 (es) | 2018-07-19 | 2021-11-05 | Biofidelity Ltd | Método mejorado de detección de secuencia de polinucleótidos |
| CN112654714B (zh) * | 2018-12-17 | 2024-10-11 | 伊卢米纳剑桥有限公司 | 用于测序的引物寡核苷酸 |
| GB201919186D0 (en) | 2019-12-23 | 2020-02-05 | Biofidelity Ltd | Simplified polynucleotide sequence detection method |
| EP4402283A4 (en) * | 2021-09-13 | 2025-11-12 | Nexgen Cancer Detection Llc | METHODS FOR DETECTING A TARGET POLYNUCLEOTIDE IN A SAMPLE |
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| CA2163393C (en) | 1994-11-30 | 2003-04-22 | Colleen Marie Nycz | Amplification and detection of mycobacteria nucleic acids |
| US5801155A (en) * | 1995-04-03 | 1998-09-01 | Epoch Pharmaceuticals, Inc. | Covalently linked oligonucleotide minor grove binder conjugates |
| US5849497A (en) | 1997-04-03 | 1998-12-15 | The Research Foundation Of State University Of New York | Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker |
| FR2779154B1 (fr) | 1998-05-27 | 2002-07-12 | Bio Merieux | Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre |
| CA2356697C (en) * | 1999-01-06 | 2010-06-22 | Cornell Research Foundation, Inc. | Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing |
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| GB0406863D0 (en) | 2004-03-26 | 2004-04-28 | Qiagen As | Nucleic acid sequencing |
| ATE476439T1 (de) | 2004-09-10 | 2010-08-15 | Human Genetic Signatures Pty | Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn) |
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2008
- 2008-08-05 US US12/186,311 patent/US8071338B2/en not_active Expired - Fee Related
- 2008-08-07 CA CA2693384A patent/CA2693384C/en not_active Expired - Fee Related
- 2008-08-07 JP JP2010519376A patent/JP5524838B2/ja not_active Expired - Fee Related
- 2008-08-07 WO PCT/EP2008/006476 patent/WO2009019008A1/en not_active Ceased
- 2008-08-07 CN CN200880102467.XA patent/CN101778952B/zh not_active Expired - Fee Related
- 2008-08-07 EP EP08785392.5A patent/EP2173910B1/en not_active Not-in-force
- 2008-08-07 ES ES08785392.5T patent/ES2562789T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2173910B1 (en) | 2015-12-09 |
| US8071338B2 (en) | 2011-12-06 |
| CN101778952A (zh) | 2010-07-14 |
| CN101778952B (zh) | 2016-05-25 |
| US20090053720A1 (en) | 2009-02-26 |
| CA2693384A1 (en) | 2009-02-12 |
| CA2693384C (en) | 2015-04-14 |
| HK1146094A1 (zh) | 2011-05-13 |
| JP5524838B2 (ja) | 2014-06-18 |
| JP2010535480A (ja) | 2010-11-25 |
| EP2173910A1 (en) | 2010-04-14 |
| WO2009019008A1 (en) | 2009-02-12 |
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