CA2693384C - Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity - Google Patents

Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity Download PDF

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Publication number
CA2693384C
CA2693384C CA2693384A CA2693384A CA2693384C CA 2693384 C CA2693384 C CA 2693384C CA 2693384 A CA2693384 A CA 2693384A CA 2693384 A CA2693384 A CA 2693384A CA 2693384 C CA2693384 C CA 2693384C
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CA
Canada
Prior art keywords
sequence
target
blocker oligonucleotide
nucleotide
oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2693384A
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English (en)
French (fr)
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CA2693384A1 (en
Inventor
Nicolas Newton
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of CA2693384A1 publication Critical patent/CA2693384A1/en
Application granted granted Critical
Publication of CA2693384C publication Critical patent/CA2693384C/en
Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2693384A 2007-08-08 2008-08-07 Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity Expired - Fee Related CA2693384C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96408907P 2007-08-08 2007-08-08
US60/964,089 2007-08-08
PCT/EP2008/006476 WO2009019008A1 (en) 2007-08-08 2008-08-07 Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity

Publications (2)

Publication Number Publication Date
CA2693384A1 CA2693384A1 (en) 2009-02-12
CA2693384C true CA2693384C (en) 2015-04-14

Family

ID=39942715

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2693384A Expired - Fee Related CA2693384C (en) 2007-08-08 2008-08-07 Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity

Country Status (7)

Country Link
US (1) US8071338B2 (OSRAM)
EP (1) EP2173910B1 (OSRAM)
JP (1) JP5524838B2 (OSRAM)
CN (1) CN101778952B (OSRAM)
CA (1) CA2693384C (OSRAM)
ES (1) ES2562789T3 (OSRAM)
WO (1) WO2009019008A1 (OSRAM)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2008282780B2 (en) 2007-08-01 2014-04-17 Dana- Farber Cancer Institute Enrichment of a target sequence
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
WO2011056687A2 (en) * 2009-10-27 2011-05-12 Swift Biosciences, Inc. Polynucleotide primers and probes
DK2545189T3 (en) * 2010-03-08 2018-04-23 Dana Farber Cancer Inst Inc COMPLETE COLD-PCR ENRICHMENT WITH REFERENCE BLOCKING SEQUENCE
CN103080310A (zh) * 2010-09-10 2013-05-01 三菱化学美迪恩斯株式会社 使用光的核酸的扩增抑制方法和高灵敏度的选择性核酸扩增方法
DK2633071T3 (en) 2010-10-27 2017-01-30 Harvard College COMPOSITIONS OF "MAINTENANCE" PRIMER DUPLEXES AND METHODS OF USE
EP2681332A1 (en) * 2011-02-28 2014-01-08 Transgenomic, Inc. Kit and method for sequencing a target dna in a mixed population
EP2691541B1 (en) * 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
US9796748B2 (en) 2011-05-02 2017-10-24 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
CN103635593B (zh) 2011-05-04 2018-03-20 生物概念股份有限公司 用于检测核酸序列变体的方法
WO2012162621A1 (en) * 2011-05-26 2012-11-29 Brandeis University Methods for suppression pcr
EP2758458A4 (en) 2011-07-10 2015-10-21 Harvard College COMPOSITIONS AND METHODS FOR SELF-ASSEMBLING POLYMERS WITH COMPLEMENTARY MACROSCOPIC AND MICROSCOPIC SCALE UNITS
EP2570487A1 (en) 2011-09-16 2013-03-20 Lexogen GmbH Nucleic acid transcription method
AU2012307282B2 (en) 2011-09-16 2018-03-15 Lexogen Gmbh Nucleic acid transcription method
US9260714B2 (en) 2011-12-02 2016-02-16 Roche Molecular Systems, Inc. Suppression of non-specific amplification with high-homology oligonucleotides
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
JP6652693B2 (ja) * 2013-04-29 2020-02-26 キアゲン ゲーエムベーハー ブロッキングオリゴヌクレオチドを用いたｄｎａ増幅の方法
CA2926722A1 (en) * 2013-10-19 2015-04-23 Trovagene, Inc. Detecting mutations in disease over time
RU2708992C2 (ru) * 2014-05-19 2019-12-12 Уильям Марш Райс Юниверсити Аллель-специфическая амплификация с использованием композиции перекрывающихся олигонуклеотидов в качестве не являющегося аллель-специфическим праймера и аллель-специфического блокатора
US12391985B2 (en) 2014-08-07 2025-08-19 Pharmassist Ltd Method of determining PIK3CA mutational status in a sample
WO2016020710A1 (en) * 2014-08-07 2016-02-11 Pharmassist Ltd Method of determining pik3ca mutational status in a sample
CN113957146A (zh) 2014-09-26 2022-01-21 詹森药业有限公司 使用fgfr突变基因组鉴定将对用fgfr抑制剂进行治疗有反应的癌症患者
US9909169B2 (en) * 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
US9657353B2 (en) 2015-06-26 2017-05-23 Great Basin Scientific, Inc. Specific detection of organisms derived from a sample
CN109563506A (zh) 2016-05-06 2019-04-02 威廉马歇莱思大学 使用随机捕获探针文库的化学计量核酸纯化
US11299739B2 (en) * 2016-06-07 2022-04-12 Cepheid Thermostable polymerase inhibitor compositions and methods
CA3046953A1 (en) 2016-12-12 2018-06-21 Dana Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
WO2019164885A1 (en) 2018-02-20 2019-08-29 William Marsh Rice University Systems and methods for allele enrichment using multiplexed blocker displacement amplification
WO2020016590A1 (en) 2018-07-19 2020-01-23 Biofidelity Ltd Improved polynucleotide sequence detection method
WO2020126595A1 (en) * 2018-12-17 2020-06-25 Illumina Cambridge Limited Primer oligonucleotide for sequencing
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
US20240401122A1 (en) * 2021-09-13 2024-12-05 Nexgen Cancer Detection Llc Methods for detecting a target polynucleotide in a sample

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2163393C (en) 1994-11-30 2003-04-22 Colleen Marie Nycz Amplification and detection of mycobacteria nucleic acids
US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
FR2779154B1 (fr) 1998-05-27 2002-07-12 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
JP2002534098A (ja) * 1999-01-06 2002-10-15 コーネル リサーチ ファンデーション インク． ゲノムシークエンシングにおける単一ヌクレオチドの多型の加速度的な同定およびクローンの整列化
EP1253205A1 (en) 2001-04-24 2002-10-30 LION Bioscience AG Method of blocking amplification of selected sequences
GB0406863D0 (en) 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
ATE476439T1 (de) 2004-09-10 2010-08-15 Human Genetic Signatures Pty Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn)

Also Published As

Publication number Publication date
EP2173910B1 (en) 2015-12-09
CA2693384A1 (en) 2009-02-12
WO2009019008A1 (en) 2009-02-12
EP2173910A1 (en) 2010-04-14
JP5524838B2 (ja) 2014-06-18
US8071338B2 (en) 2011-12-06
ES2562789T3 (es) 2016-03-08
CN101778952B (zh) 2016-05-25
US20090053720A1 (en) 2009-02-26
HK1146094A1 (zh) 2011-05-13
CN101778952A (zh) 2010-07-14
JP2010535480A (ja) 2010-11-25

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