ES2533555T3 - Proteínas de seda de araña y métodos para producir proteínas de seda de araña - Google Patents
Proteínas de seda de araña y métodos para producir proteínas de seda de araña Download PDFInfo
- Publication number
- ES2533555T3 ES2533555T3 ES06835913.2T ES06835913T ES2533555T3 ES 2533555 T3 ES2533555 T3 ES 2533555T3 ES 06835913 T ES06835913 T ES 06835913T ES 2533555 T3 ES2533555 T3 ES 2533555T3
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- Prior art keywords
- amino acid
- acid residues
- seq
- protein
- segment
- Prior art date
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Links
- 108090000623 proteins and genes Proteins 0.000 title abstract description 46
- 102000004169 proteins and genes Human genes 0.000 title abstract description 44
- 238000000034 method Methods 0.000 title description 10
- 229920001872 Spider silk Polymers 0.000 title description 9
- 125000000539 amino acid group Chemical group 0.000 abstract description 54
- 108010022355 Fibroins Proteins 0.000 abstract description 31
- 239000012634 fragment Substances 0.000 abstract description 23
- 150000001413 amino acids Chemical class 0.000 abstract description 18
- 239000003795 chemical substances by application Substances 0.000 abstract description 12
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 3
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- 210000004027 cell Anatomy 0.000 description 18
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- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 15
- 241000328437 Euprosthenops australis Species 0.000 description 12
- 239000002158 endotoxin Substances 0.000 description 12
- 229920006008 lipopolysaccharide Polymers 0.000 description 12
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- 108020004707 nucleic acids Proteins 0.000 description 11
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- 241000023938 Argiope trifasciata Species 0.000 description 2
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- 241000023953 Latrodectus geometricus Species 0.000 description 2
- 241001387337 Latrodectus hesperus Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 2
- 241000210679 Nephila inaurata madagascariensis Species 0.000 description 2
- 241000332308 Pisauridae Species 0.000 description 2
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- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
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- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 101710191870 Actin-depolymerizing factor 1 Proteins 0.000 description 1
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- 241001157789 Araneus bicentenarius Species 0.000 description 1
- 241001598873 Argiope amoena Species 0.000 description 1
- 241000023936 Argiope aurantia Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241001221772 Cyrtophora moluccensis Species 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241001499232 Gasteracantha cancriformis Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 108090001007 Interleukin-8 Proteins 0.000 description 1
- 125000002066 L-histidyl group Chemical group [H]N1C([H])=NC(C([H])([H])[C@](C(=O)[*])([H])N([H])[H])=C1[H] 0.000 description 1
- 241000239218 Limulus Species 0.000 description 1
- 241000332328 Macrothele holsti Species 0.000 description 1
- 241000699667 Mus spretus Species 0.000 description 1
- 241001221743 Nephila pilipes Species 0.000 description 1
- 241001221740 Octonoba varians Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000332821 Psechrus sinensis Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 241000023957 Tetragnatha kauaiensis Species 0.000 description 1
- 241000023959 Tetragnatha versicolor Species 0.000 description 1
- 108090000190 Thrombin Proteins 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
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- 231100000252 nontoxic Toxicity 0.000 description 1
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- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
- C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F4/00—Monocomponent artificial filaments or the like of proteins; Manufacture thereof
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Insects & Arthropods (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Textile Engineering (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- Virology (AREA)
- Developmental Biology & Embryology (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0502932 | 2005-12-30 | ||
| SE0502932 | 2005-12-30 | ||
| PCT/SE2006/001505 WO2007078239A2 (en) | 2005-12-30 | 2006-12-28 | Spider silk proteins and methods for producing spider silk proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2533555T3 true ES2533555T3 (es) | 2015-04-13 |
Family
ID=37836847
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES06835913.2T Active ES2533555T3 (es) | 2005-12-30 | 2006-12-28 | Proteínas de seda de araña y métodos para producir proteínas de seda de araña |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US8173772B2 (https=) |
| EP (2) | EP1976868B1 (https=) |
| JP (1) | JP5192391B2 (https=) |
| CN (1) | CN101395178B (https=) |
| AU (1) | AU2006333611B2 (https=) |
| CA (1) | CA2635660C (https=) |
| DK (1) | DK1976868T3 (https=) |
| ES (1) | ES2533555T3 (https=) |
| RU (1) | RU2444527C2 (https=) |
| WO (1) | WO2007078239A2 (https=) |
Families Citing this family (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008154547A2 (en) * | 2007-06-11 | 2008-12-18 | The Regents Of The University Of California | Spider silk dragline polynucleotides, polypeptides and methods of use thereof |
| EP2243792A1 (en) | 2009-04-22 | 2010-10-27 | Spiber Technologies AB | Methods of producing polymers of spider silk proteins |
| EP2421890B1 (en) * | 2009-04-22 | 2015-08-26 | Spiber Technologies AB | Method of producing polymers of spider silk proteins |
| CN101724920B (zh) * | 2009-11-13 | 2011-04-27 | 东华大学 | 干纺制备再生蚕丝纤维的方法 |
| US9233067B2 (en) | 2009-11-30 | 2016-01-12 | Amsilk Gmbh | Silk particles for controlled and sustained delivery of compounds |
| WO2011113446A1 (en) * | 2010-03-17 | 2011-09-22 | Amsilk Gmbh | Method for production of polypeptide containing fibres |
| JP5726282B2 (ja) | 2010-03-18 | 2015-05-27 | スパイバー テクノロジーズ アーベーＳｐｉｂｅｒ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ａｂ | タンパク質及びポリペプチドの産生 |
| CA2795541C (en) * | 2010-04-12 | 2020-05-26 | Spiber Technologies Ab | Spider silk protein scaffolds for eukaryotic cell culture |
| RU2624036C2 (ru) * | 2010-10-27 | 2017-06-30 | Спибер Текнолоджиз Аб | Структуры слитого белка шелка пауков для связывания с органической мишенью |
| RU2451023C1 (ru) * | 2010-11-25 | 2012-05-20 | Владимир Григорьевич Богуш | Способ получения рекомбинантного белка паутины, слитый белок, рекомбинантная днк, вектор экспрессии, клетка-хозяин и штаммы-продуценты |
| US9617315B2 (en) | 2011-06-01 | 2017-04-11 | Spiber Inc. | Artificial polypeptide fiber and method for producing the same |
| EP2774934B1 (en) | 2011-11-02 | 2017-11-29 | Spiber Inc. | Polypeptide solution, artificial polypeptide fiber production method using same, and polypeptide refining method |
| WO2013065651A1 (ja) * | 2011-11-02 | 2013-05-10 | スパイバー株式会社 | タンパク質溶液及びこれを用いたタンパク質繊維の製造方法 |
| EP2844360B1 (en) | 2012-05-02 | 2018-12-19 | Spiber Technologies AB | Spider silk fusion protein structures without repetitive fragment for binding to an organic target |
| US9856308B2 (en) | 2012-05-02 | 2018-01-02 | Spiber Technologies Ab | Spider silk fusion protein structures incorporating immunoglobulin fragments as affinity ligands |
| WO2014002605A1 (ja) | 2012-06-28 | 2014-01-03 | スパイバー株式会社 | 原着タンパク質繊維及びその製造方法 |
| WO2014052975A1 (en) * | 2012-09-28 | 2014-04-03 | Utah State University | Expression systems and methods of producing spider silk proteins |
| US10899803B2 (en) | 2012-10-17 | 2021-01-26 | Nanyang Technological University | Compounds and methods for the production of suckerin and uses thereof |
| CA2924343C (en) | 2013-09-17 | 2025-09-16 | Bolt Threads, Inc. | PROCESSES AND COMPOSITIONS FOR SYNTHESIZING IMPROVED SILK FIBERS |
| EP3281948B1 (en) | 2015-04-09 | 2020-06-10 | Spiber Inc. | Polar solvent solution and production method thereof |
| EP3281949A4 (en) | 2015-04-09 | 2018-08-22 | Spiber Inc. | Polar solvent solution and production method thereof |
| EP3307765B1 (en) * | 2015-06-11 | 2024-04-10 | Bolt Threads, Inc. | Recombinant protein fiber yarns with improved properties |
| CN105031723B (zh) * | 2015-06-23 | 2017-05-24 | 上海交通大学 | 基于蜘蛛丝蛋白的温敏性水凝胶 |
| JP6863976B2 (ja) * | 2015-06-26 | 2021-04-21 | スピベル テクノロジーズ アクティエボラーグ | 環状ｒｇｄ細胞結合モチーフ及びその使用 |
| KR102751752B1 (ko) * | 2015-08-10 | 2025-01-09 | 시비스 머테리얼 사이언스 리미티드 | 합성 드래그라인 거미 명주를 제조하기 위한 조성물 및 방법 |
| JP2018159137A (ja) | 2015-08-20 | 2018-10-11 | 国立研究開発法人理化学研究所 | クモ糸様構造を有するポリペプチド繊維の製造方法 |
| WO2017138002A1 (en) | 2016-02-11 | 2017-08-17 | Seevix Material Sciences Ltd. | Composite materials comprising synthetic dragline spider silk |
| US11484624B2 (en) | 2016-05-16 | 2022-11-01 | Spiber Technologies Ab | Spider silk coating of solid surfaces |
| EP3246051A1 (en) | 2016-05-16 | 2017-11-22 | Spiber Technologies AB | Spider silk coating of solid surfaces |
| EP3263593A1 (en) | 2016-07-01 | 2018-01-03 | Anna Rising | Engineered spider silk proteins and uses thereof |
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| CN109912720B (zh) * | 2019-03-14 | 2021-12-07 | 天津大学 | 一种蛛丝蛋白的设计合成方法和纺丝 |
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| TW336938B (en) * | 1992-05-21 | 1998-07-21 | Pfizer | Calcium channel blocking polypeptides from filistata hibernalis |
| EP1413585A3 (en) | 1993-06-15 | 2004-08-04 | E.I. Du Pont De Nemours And Company | Novel, recombinantly produced spider silk analogs |
| US5733771A (en) * | 1994-03-14 | 1998-03-31 | University Of Wyoming | cDNAs encoding minor ampullate spider silk proteins |
| IL123398A0 (en) | 1995-08-22 | 1998-09-24 | Agricola Tech Inc | Cloning methods for high strength spider silk proteins |
| US5994099A (en) * | 1997-12-31 | 1999-11-30 | The University Of Wyoming | Extremely elastic spider silk protein and DNA coding therefor |
| US6620917B1 (en) | 2000-01-20 | 2003-09-16 | The United States Of America As Represented By The Secretary Of The Army | Method for the purification and aqueous fiber spinning of spider silks and other structural proteins |
| US6608242B1 (en) | 2000-05-25 | 2003-08-19 | E. I. Du Pont De Nemours And Company | Production of silk-like proteins in plants |
| DE10113781A1 (de) | 2000-06-09 | 2001-12-13 | Ipk Inst Fuer Pflanzengenetik | Synthetische Spinnenseidenproteine und deren Expression in transgenen Pflanzen |
| WO2002099082A2 (en) * | 2001-06-06 | 2002-12-12 | University Of Wyoming | Expression of spider silk proteins in higher plants |
| JP2005502347A (ja) * | 2001-08-29 | 2005-01-27 | ユニバーシティ オブ ワイオミング | クモ糸タンパク質をコードする核酸、ポリペプチド、抗体とそれらの使用方法 |
| AU2003201513A1 (en) | 2002-01-11 | 2003-07-24 | Nexia Biotechnologies, Inc. | Methods of producing silk polypeptides and products thereof |
| WO2003060099A2 (en) | 2002-01-11 | 2003-07-24 | Nexia Biotechnologies, Inc. | Methods and apparatus for spinning spider silk protein |
| AU2003202330A1 (en) | 2002-01-11 | 2003-07-24 | Nexia Biotechnologies, Inc. | Recovery of biofilament proteins from biological fluids |
| GB0218977D0 (en) | 2002-08-15 | 2002-09-25 | Univ York | Polypeptide |
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| US7060260B2 (en) | 2003-02-20 | 2006-06-13 | E.I. Du Pont De Nemours And Company | Water-soluble silk proteins in compositions for skin care, hair care or hair coloring |
| FR2871822B1 (fr) | 2004-06-17 | 2007-10-05 | Icopal Sas Soc Par Actions Sim | Isolant mince reflechissant pour sous-toiture |
| EP1609801A1 (en) * | 2004-06-25 | 2005-12-28 | Technische Universität München | Proteins of natural origin and materials made therefrom |
| CN101018806B (zh) | 2004-07-22 | 2014-05-07 | Am丝绸有限责任公司 | 重组蜘蛛丝蛋白 |
| WO2006083514A2 (en) | 2005-01-14 | 2006-08-10 | The Florida International University Board Of Trustees | Transgenic production of spider silk |
| AU2006286753B2 (en) | 2005-08-29 | 2011-08-18 | Amsilk Gmbh | Modified spider silk proteins |
| EP1852470A1 (en) | 2006-05-03 | 2007-11-07 | Technische Universität München | Multilayer Silk Protein Films |
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| RU2444527C2 (ru) | 2012-03-10 |
| US8618255B2 (en) | 2013-12-31 |
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| US20120264914A1 (en) | 2012-10-18 |
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| CA2635660A1 (en) | 2007-07-12 |
| RU2008131298A (ru) | 2010-02-10 |
| WO2007078239A3 (en) | 2007-12-21 |
| EP1976868B1 (en) | 2015-02-18 |
| US20120329992A1 (en) | 2012-12-27 |
| EP2899204A1 (en) | 2015-07-29 |
| JP5192391B2 (ja) | 2013-05-08 |
| WO2007078239A2 (en) | 2007-07-12 |
| AU2006333611B2 (en) | 2012-04-26 |
| AU2006333611A1 (en) | 2007-07-12 |
| EP1976868A2 (en) | 2008-10-08 |
| US8173772B2 (en) | 2012-05-08 |
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