ES2403591T3 - ADN polimerasas mutantes y métodos relacionados - Google Patents
ADN polimerasas mutantes y métodos relacionados Download PDFInfo
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- ES2403591T3 ES2403591T3 ES08784745T ES08784745T ES2403591T3 ES 2403591 T3 ES2403591 T3 ES 2403591T3 ES 08784745 T ES08784745 T ES 08784745T ES 08784745 T ES08784745 T ES 08784745T ES 2403591 T3 ES2403591 T3 ES 2403591T3
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- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
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- 230000000984 immunochemical effect Effects 0.000 description 1
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- 238000002955 isolation Methods 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
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- 125000000468 ketone group Chemical group 0.000 description 1
- GDBQQVLCIARPGH-ULQDDVLXSA-N leupeptin Chemical compound CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C=O)CCCN=C(N)N GDBQQVLCIARPGH-ULQDDVLXSA-N 0.000 description 1
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- 235000019341 magnesium sulphate Nutrition 0.000 description 1
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- 229910021645 metal ion Inorganic materials 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
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- 238000007069 methylation reaction Methods 0.000 description 1
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- 230000003505 mutagenic effect Effects 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- YFCUZWYIPBUQBD-ZOWNYOTGSA-N n-[(3s)-7-amino-1-chloro-2-oxoheptan-3-yl]-4-methylbenzenesulfonamide;hydron;chloride Chemical compound Cl.CC1=CC=C(S(=O)(=O)N[C@@H](CCCCN)C(=O)CCl)C=C1 YFCUZWYIPBUQBD-ZOWNYOTGSA-N 0.000 description 1
- 210000004897 n-terminal region Anatomy 0.000 description 1
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- 230000002018 overexpression Effects 0.000 description 1
- PSWJVKKJYCAPTI-UHFFFAOYSA-N oxido-oxo-phosphonophosphanylphosphanium Chemical compound OP(O)(=O)PP(=O)=O PSWJVKKJYCAPTI-UHFFFAOYSA-N 0.000 description 1
- LDCYZAJDBXYCGN-UHFFFAOYSA-N oxitriptan Natural products C1=C(O)C=C2C(CC(N)C(O)=O)=CNC2=C1 LDCYZAJDBXYCGN-UHFFFAOYSA-N 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 150000004713 phosphodiesters Chemical class 0.000 description 1
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- 238000009666 routine test Methods 0.000 description 1
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- 239000007787 solid Substances 0.000 description 1
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- 238000010561 standard procedure Methods 0.000 description 1
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- 125000000446 sulfanediyl group Chemical group *S* 0.000 description 1
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- 229960002180 tetracycline Drugs 0.000 description 1
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- 150000003522 tetracyclines Chemical class 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US94973207P | 2007-07-13 | 2007-07-13 | |
| US949732P | 2007-07-13 | ||
| PCT/EP2008/005714 WO2009010251A2 (en) | 2007-07-13 | 2008-07-12 | Mutant dna polymerases and related methods |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2403591T3 true ES2403591T3 (es) | 2013-05-20 |
Family
ID=39800730
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES08784745T Active ES2403591T3 (es) | 2007-07-13 | 2008-07-12 | ADN polimerasas mutantes y métodos relacionados |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8026091B2 (OSRAM) |
| EP (1) | EP2164955B1 (OSRAM) |
| JP (1) | JP5612469B2 (OSRAM) |
| CN (1) | CN102099464B (OSRAM) |
| CA (1) | CA2693657C (OSRAM) |
| ES (1) | ES2403591T3 (OSRAM) |
| WO (1) | WO2009010251A2 (OSRAM) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2706999C (en) | 2007-11-28 | 2013-01-22 | F. Hoffmann-La Roche Ag | Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability |
| US10150990B2 (en) | 2008-04-21 | 2018-12-11 | Roche Molecular Systems, Inc. | Ribonucleotide tag nucleic acid detection |
| EP2582802B1 (en) | 2010-06-18 | 2016-01-13 | Roche Diagnostics GmbH | Dna polymerases with increased 3'-mismatch discrimination |
| JP5926246B2 (ja) * | 2010-06-18 | 2016-05-25 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ |
| JP5926247B2 (ja) | 2010-06-18 | 2016-05-25 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ |
| US10597643B2 (en) | 2016-04-29 | 2020-03-24 | Omniome, Inc. | Polymerases engineered to reduce nucleotide-independent DNA binding |
| US10584379B2 (en) * | 2016-04-29 | 2020-03-10 | Omniome, Inc. | Method of nucleic acid sequence determination |
| EP3850086A1 (en) * | 2018-09-13 | 2021-07-21 | F. Hoffmann-La Roche AG | Mutant dna polymerase(s) with improved strand displacement ability |
| CN109486788B (zh) * | 2018-10-26 | 2021-10-22 | 南京市胸科医院 | 一种突变体dna聚合酶及其制备方法和应用 |
| WO2021217597A1 (zh) * | 2020-04-30 | 2021-11-04 | 广州达安基因股份有限公司 | 一种具有高扩增活性的耐热dna聚合酶突变体 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5374553A (en) * | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| JP3112148B2 (ja) | 1995-05-31 | 2000-11-27 | 東洋紡績株式会社 | 核酸の増幅方法およびその試薬 |
| CA2241170A1 (en) * | 1995-12-27 | 1997-07-10 | Takara Shuzo Co., Ltd. | Novel dna polymerase |
| US6329178B1 (en) | 2000-01-14 | 2001-12-11 | University Of Washington | DNA polymerase mutant having one or more mutations in the active site |
| US7179590B2 (en) | 2000-04-18 | 2007-02-20 | Roche Molecular Systems, Inc | High temperature reverse transcription using mutant DNA polymerases |
| US7148049B2 (en) * | 2002-04-02 | 2006-12-12 | Roche Molecular Systems, Inc. | Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity |
| EP1801213A3 (en) | 2003-11-03 | 2007-10-24 | Medical Research Council | Polymerase |
| JP2006197947A (ja) * | 2006-04-26 | 2006-08-03 | Toyobo Co Ltd | エキソヌクレアーゼ活性が低減された耐熱性ｄｎａポリメラーゼおよびその用途 |
-
2008
- 2008-07-09 US US12/170,110 patent/US8026091B2/en not_active Expired - Fee Related
- 2008-07-12 CA CA2693657A patent/CA2693657C/en not_active Expired - Fee Related
- 2008-07-12 ES ES08784745T patent/ES2403591T3/es active Active
- 2008-07-12 JP JP2010515427A patent/JP5612469B2/ja not_active Expired - Fee Related
- 2008-07-12 EP EP08784745A patent/EP2164955B1/en not_active Not-in-force
- 2008-07-12 WO PCT/EP2008/005714 patent/WO2009010251A2/en not_active Ceased
- 2008-07-12 CN CN2008800244615A patent/CN102099464B/zh not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO2009010251A2 (en) | 2009-01-22 |
| US8026091B2 (en) | 2011-09-27 |
| WO2009010251A3 (en) | 2009-03-05 |
| EP2164955B1 (en) | 2013-03-06 |
| JP5612469B2 (ja) | 2014-10-22 |
| CN102099464A (zh) | 2011-06-15 |
| US20090137000A1 (en) | 2009-05-28 |
| JP2010532979A (ja) | 2010-10-21 |
| EP2164955A2 (en) | 2010-03-24 |
| WO2009010251A8 (en) | 2010-04-08 |
| HK1157399A1 (en) | 2012-06-29 |
| CA2693657A1 (en) | 2009-01-22 |
| CA2693657C (en) | 2013-09-10 |
| CN102099464B (zh) | 2013-10-30 |
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