ES2311508T3 - Receptores gustativos t1r y genes que codifican dichos receptores. - Google Patents
Receptores gustativos t1r y genes que codifican dichos receptores. Download PDFInfo
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- ES2311508T3 ES2311508T3 ES01914732T ES01914732T ES2311508T3 ES 2311508 T3 ES2311508 T3 ES 2311508T3 ES 01914732 T ES01914732 T ES 01914732T ES 01914732 T ES01914732 T ES 01914732T ES 2311508 T3 ES2311508 T3 ES 2311508T3
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
- C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
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- Gastroenterology & Hepatology (AREA)
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- Genetics & Genomics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Endocrinology (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Seasonings (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Steroid Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Confectionery (AREA)
- General Preparation And Processing Of Foods (AREA)
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| US195536P | 2000-04-07 | ||
| US20984000P | 2000-06-06 | 2000-06-06 | |
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| US21421300P | 2000-06-23 | 2000-06-23 | |
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| US22644800P | 2000-08-17 | 2000-08-17 | |
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| US25922701P | 2001-01-03 | 2001-01-03 | |
| US259227P | 2001-01-03 | ||
| PCT/US2001/007265 WO2001066563A2 (en) | 2000-03-07 | 2001-03-07 | T1r taste receptors and genes encoding same |
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| ES08152887T Expired - Lifetime ES2338497T3 (es) | 2000-03-07 | 2001-03-07 | Receptores gustativos t1r1 y genes que codifican los mismos. |
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| ES10180329T Expired - Lifetime ES2396681T3 (es) | 2000-03-07 | 2001-03-07 | Dominios de receptores gustativos T1R3 y genes que los codifican |
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Families Citing this family (80)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7588900B2 (en) | 2001-07-03 | 2009-09-15 | The Regents Of The University Of California | Mammalian sweet and amino acid heterodimeric taste receptors |
| US7402400B2 (en) | 2001-07-03 | 2008-07-22 | Regents Of The University Of California | Mammalian sweet taste receptors |
| CN1178952C (zh) | 1998-07-28 | 2004-12-08 | 加利福尼亚大学董事会 | 编码与感觉转导有关的g-蛋白偶联受体的核酸 |
| TW201006846A (en) | 2000-03-07 | 2010-02-16 | Senomyx Inc | T1R taste receptor and genes encidung same |
| JP2003531614A (ja) * | 2000-04-28 | 2003-10-28 | ワーナー−ランバート・カンパニー、リミテッド、ライアビリティ、カンパニー | 炭水化物化合物および他の甘味物質の感知に関連する遺伝子および配列変異 |
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| CA2408140A1 (en) * | 2000-05-22 | 2001-11-29 | Incyte Genomics, Inc. | G-protein coupled receptors |
| DE60138808D1 (de) * | 2000-06-16 | 2009-07-09 | Incyte Corp | G-protein gekoppelte rezeptoren |
| US20040039170A1 (en) * | 2000-09-25 | 2004-02-26 | Sophia Kossida | Regulation of human g protein-coupled receptor |
| TW201022287A (en) | 2001-01-03 | 2010-06-16 | Senomyx Inc | T1R taste receptors and genes encoding same |
| US7763431B1 (en) | 2001-03-07 | 2010-07-27 | Senomyx, Inc. | Binding assays that use the T1R2/T1R3 (sweet) taste receptor to identify compounds that elicit or modulate sweet taste |
| US20080244761A1 (en) * | 2001-03-07 | 2008-10-02 | Senomyx, Inc. | T1r hetero-oligomeric taste receptors and cell lines that express said receptors and use thereof for identification of taste compounds |
| US7301009B2 (en) * | 2001-06-26 | 2007-11-27 | Senomyx, Inc. | Isolated (T1R1/T1R3) umami taste receptors that respond to umami taste stimuli |
| US6955887B2 (en) | 2001-03-30 | 2005-10-18 | Senomyx, Inc. | Use of T1R hetero-oligomeric taste receptor to screen for compounds that modulate taste signaling |
| US7368285B2 (en) | 2001-03-07 | 2008-05-06 | Senomyx, Inc. | Heteromeric umami T1R1/T1R3 taste receptors and isolated cells that express same |
| US7309577B2 (en) * | 2001-03-07 | 2007-12-18 | Senomyx, Inc. | Binding assays that use the T1R1/T1R3 (umami) taste receptor to identify compounds that elicit or modulate umami taste |
| EP1572871A4 (en) * | 2001-04-20 | 2007-11-14 | Sinai School Medicine | T1R3, A NEW TASTE RECEPTOR |
| US7803982B2 (en) | 2001-04-20 | 2010-09-28 | The Mount Sinai School Of Medicine Of New York University | T1R3 transgenic animals, cells and related methods |
| CN100489102C (zh) * | 2001-06-26 | 2009-05-20 | 塞诺米克斯公司 | T1r异源寡聚的味觉受体和表达所述受体的细胞系及其在鉴定味觉化合物中的用途 |
| DK2327985T3 (en) * | 2001-06-26 | 2016-09-05 | Senomyx Inc | H1 Oligomeric T1R Taste Receptors and Cell Lines Expressing the Receptors, and Their Use to Identify Taste Compounds |
| US20040029789A1 (en) * | 2001-07-05 | 2004-02-12 | Anderson David W. | Novel proteins and nucleic acids encoding same |
| WO2003008627A2 (en) | 2001-07-20 | 2003-01-30 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Phenylthiocarbamide (ptc) taste receptor |
| WO2003025137A2 (en) * | 2001-09-18 | 2003-03-27 | Irm, Llc | Sweet taste receptors |
| CA2486423A1 (en) * | 2002-05-20 | 2003-12-11 | Tvd Taste Virtual Dimensions, Inc. | Antibodies and their use for the modulation of taste sensation |
| AU2004258071A1 (en) | 2003-06-19 | 2005-01-27 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Variants of human taste receptor genes |
| NZ545747A (en) | 2003-08-06 | 2010-06-25 | Senomyx Inc | T1R hetero-oligomeric taste receptors, cell lines that express said receptors, and taste compounds |
| US20050244810A1 (en) * | 2003-09-29 | 2005-11-03 | Egan Josephine M | Taste signaling in gastrointestinal cells |
| US20050106571A1 (en) | 2003-10-02 | 2005-05-19 | The Regents Of The University Of California | Mammalian T1R3 sweet taste receptors |
| US20060045953A1 (en) * | 2004-08-06 | 2006-03-02 | Catherine Tachdjian | Aromatic amides and ureas and their uses as sweet and/or umami flavor modifiers, tastants and taste enhancers |
| WO2006068745A1 (en) | 2004-12-22 | 2006-06-29 | Cargill, Incorporated | Methods for determining cellular response to stimuli |
| US20060257543A1 (en) * | 2005-02-04 | 2006-11-16 | Catherine Tachdjian | Molecules comprising linked organic moieties as flavor modifiers for comestible compositions |
| CA2597134C (en) * | 2005-02-04 | 2015-05-26 | Senomyx, Inc. | Compounds comprising linked heteroaryl moieties and their use as novel umami flavor modifiers, tastants and taste enhancers for comestible compositions |
| US8796441B2 (en) | 2005-04-13 | 2014-08-05 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Human sweet and umami taste receptor variants |
| US7851005B2 (en) | 2005-05-23 | 2010-12-14 | Cadbury Adams Usa Llc | Taste potentiator compositions and beverages containing same |
| US7851006B2 (en) | 2005-05-23 | 2010-12-14 | Cadbury Adams Usa Llc | Taste potentiator compositions and beverages containing same |
| CN101179943B (zh) | 2005-05-23 | 2013-06-26 | 卡夫食品环球品牌有限责任公司 | 填充液体的咀嚼型胶基糖组合物 |
| JP4750184B2 (ja) | 2005-05-23 | 2011-08-17 | キャドバリー アダムス ユーエスエー エルエルシー | 味覚増強組成物並びに味覚増強組成物を含有する、食用菓子製品及びチューインガム製品 |
| TW200715993A (en) * | 2005-06-15 | 2007-05-01 | Senomyx Inc | Bis-aromatic amides and their uses as sweet flavor modifiers, tastants, and taste enhancers |
| US20110097741A1 (en) * | 2006-04-20 | 2011-04-28 | Jay Patrick Slack | Partial t1r2 nucleic acid sequence, receptor protein and its use in screening assays |
| EP2010009B1 (en) | 2006-04-21 | 2017-06-14 | Senomyx, Inc. | Processes for preparing solid flavorant compositions |
| US20070259875A1 (en) * | 2006-04-28 | 2007-11-08 | Atwal Karnail S | Triaryl substituted imidazole derivatives and taste-inhibiting uses thereof |
| WO2008008224A2 (en) * | 2006-07-10 | 2008-01-17 | Duke University | Sour taste receptor compositions and methods |
| CA2666825A1 (en) * | 2006-11-15 | 2008-05-22 | Redpoint Bio Corporation | Spicematrix technology for taste compound identification |
| US7928111B2 (en) * | 2007-06-08 | 2011-04-19 | Senomyx, Inc. | Compounds including substituted thienopyrimidinone derivatives as ligands for modulating chemosensory receptors |
| US8633186B2 (en) | 2007-06-08 | 2014-01-21 | Senomyx Inc. | Modulation of chemosensory receptors and ligands associated therewith |
| US9603848B2 (en) * | 2007-06-08 | 2017-03-28 | Senomyx, Inc. | Modulation of chemosensory receptors and ligands associated therewith |
| GB0713297D0 (en) * | 2007-07-10 | 2007-08-15 | Cadbury Schweppes Plc | Chocolate compositions having improved flavour characteristics |
| US9714941B2 (en) * | 2007-07-31 | 2017-07-25 | Massachusetts Institute Of Technology | Bio-sensing nanodevice |
| ES2647947T3 (es) | 2008-07-31 | 2017-12-27 | Senomyx, Inc. | Procesos y productos intermedios para la preparación de potenciadores del sabor dulce |
| EP2328426B1 (en) | 2008-07-31 | 2014-10-01 | Senomyx, Inc. | Compositions comrpising sweetness enhancers and methods of making them |
| WO2010014903A1 (en) | 2008-07-31 | 2010-02-04 | Massachusetts Institute Of Technology | Multiplexed olfactory receptor-based microsurface plasmon polariton detector |
| US20120184465A1 (en) * | 2009-07-20 | 2012-07-19 | Verdezyne, Inc. | Combinatorial methods for optimizing engineered microorganism function |
| WO2011067202A1 (en) | 2009-12-02 | 2011-06-09 | Unilever Nv | Improved method for screening a potential modulator compound of a taste receptor |
| US9000054B2 (en) | 2010-08-12 | 2015-04-07 | Senomyx, Inc. | Method of improving stability of sweet enhancer and composition containing stabilized sweet enhancer |
| US8956859B1 (en) | 2010-08-13 | 2015-02-17 | Aviex Technologies Llc | Compositions and methods for determining successful immunization by one or more vaccines |
| AU2012249720B2 (en) | 2011-04-29 | 2015-03-26 | Intercontinental Great Brands Llc | Encapsulated acid, method for the preparation thereof, and chewing gum comprising same |
| WO2013022948A1 (en) | 2011-08-08 | 2013-02-14 | The Coca-Cola Company | Cell lines comprising endogenous taste receptors and their uses |
| US9456916B2 (en) | 2013-03-12 | 2016-10-04 | Medibotics Llc | Device for selectively reducing absorption of unhealthy food |
| US9042596B2 (en) | 2012-06-14 | 2015-05-26 | Medibotics Llc | Willpower watch (TM)—a wearable food consumption monitor |
| US9536449B2 (en) | 2013-05-23 | 2017-01-03 | Medibotics Llc | Smart watch and food utensil for monitoring food consumption |
| US10314492B2 (en) | 2013-05-23 | 2019-06-11 | Medibotics Llc | Wearable spectroscopic sensor to measure food consumption based on interaction between light and the human body |
| US9254099B2 (en) | 2013-05-23 | 2016-02-09 | Medibotics Llc | Smart watch and food-imaging member for monitoring food consumption |
| US9442100B2 (en) | 2013-12-18 | 2016-09-13 | Medibotics Llc | Caloric intake measuring system using spectroscopic and 3D imaging analysis |
| RU2666534C2 (ru) | 2012-08-06 | 2018-09-11 | Сеномикс, Инк. | Модификатор сладкого вкуса и аромата |
| JO3155B1 (ar) | 2013-02-19 | 2017-09-20 | Senomyx Inc | معدِّل نكهة حلوة |
| US9067070B2 (en) | 2013-03-12 | 2015-06-30 | Medibotics Llc | Dysgeusia-inducing neurostimulation for modifying consumption of a selected nutrient type |
| US9011365B2 (en) | 2013-03-12 | 2015-04-21 | Medibotics Llc | Adjustable gastrointestinal bifurcation (AGB) for reduced absorption of unhealthy food |
| US9804157B1 (en) | 2013-03-15 | 2017-10-31 | Senomyx, Inc. | Screening assays to identify compounds which modulate T1R associated taste modalities which eliminate false positives |
| US9529385B2 (en) | 2013-05-23 | 2016-12-27 | Medibotics Llc | Smart watch and human-to-computer interface for monitoring food consumption |
| US9826662B2 (en) | 2013-12-12 | 2017-11-21 | General Electric Company | Reusable phase-change thermal interface structures |
| US10660571B2 (en) * | 2013-12-19 | 2020-05-26 | Kent State University | Thermochromic fabrics utilizing cholesteric liquid crystal material |
| EP3104712B1 (en) | 2014-02-12 | 2020-05-06 | Firmenich Incorporated | Improved process for the synthesis of substituted 1-benzyl-3-(1-(isoxazol-4-ylmethyl)-1h-pyrazol-4-yl)imidazolidine-2,4-diones |
| WO2015163905A1 (en) | 2014-04-25 | 2015-10-29 | General Mills, Inc. | Food products having sweetness enhancer |
| JP6383704B2 (ja) * | 2015-07-02 | 2018-08-29 | 日立オートモティブシステムズ株式会社 | 電池制御装置 |
| JP7453210B2 (ja) | 2018-08-07 | 2024-03-19 | フィルメニッヒ インコーポレイテッド | ５－置換４－アミノ－１Ｈ－ベンゾ［ｃ］［１，２，６］チアジアジン２，２－ジオキシド並びにその配合物及び使用 |
| US11783920B2 (en) | 2019-02-06 | 2023-10-10 | Tata Consultancy Services Limited | System and method for evaluation of at least one potential tastant from a plurality of tastants |
| WO2021150686A1 (en) | 2020-01-22 | 2021-07-29 | Firmenich Incorporated | High-sensitivity detection of gpcr activity and uses thereof |
| CA3186321A1 (en) | 2020-06-25 | 2021-12-30 | Mycotechnology, Inc. | Sweet protein from truffle |
| US20240345068A1 (en) | 2023-03-01 | 2024-10-17 | Alcheme Bio Inc. | Systems, methods, and compositions for production of cellular agriculture products |
| CN116554350B (zh) * | 2023-04-26 | 2024-01-09 | 之江实验室 | 基于人类甜味受体蛋白的生物传感器及其应用 |
Family Cites Families (91)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US788470A (en) * | 1903-06-16 | 1905-04-25 | Edouard Jehn | Changing mechanism for picture-exhibitors. |
| US1733847A (en) * | 1927-03-11 | 1929-10-29 | Variety Fire Door Company | Door-latch retainer |
| JPS5825980B2 (ja) | 1976-02-12 | 1983-05-31 | ヤマサ醤油株式会社 | サイクリックヌクレオチドの定量法 |
| US4391904A (en) | 1979-12-26 | 1983-07-05 | Syva Company | Test strip kits in immunoassays and compositions therein |
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4376110A (en) | 1980-08-04 | 1983-03-08 | Hybritech, Incorporated | Immunometric assays using monoclonal antibodies |
| US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
| US4517288A (en) | 1981-01-23 | 1985-05-14 | American Hospital Supply Corp. | Solid phase system for ligand assay |
| US4661768A (en) * | 1983-09-14 | 1987-04-28 | Johnson Service Company | Capacitance transducing method and apparatus |
| US5506337A (en) | 1985-03-15 | 1996-04-09 | Antivirals Inc. | Morpholino-subunit combinatorial library and method |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| CA1291031C (en) | 1985-12-23 | 1991-10-22 | Nikolaas C.J. De Jaeger | Method for the detection of specific binding agents and their correspondingbindable substances |
| US5010175A (en) | 1988-05-02 | 1991-04-23 | The Regents Of The University Of California | General method for producing and selecting peptides with specific properties |
| US5464764A (en) | 1989-08-22 | 1995-11-07 | University Of Utah Research Foundation | Positive-negative selection methods and vectors |
| RU2128227C1 (ru) | 1989-12-22 | 1999-03-27 | Апплайд Резеч Системз АРС Холдинг Н.В. (NL) (Антильские острова) | Способ активации транскрипционно-молчащего гена |
| US5272071A (en) | 1989-12-22 | 1993-12-21 | Applied Research Systems Ars Holding N.V. | Method for the modification of the expression characteristics of an endogenous gene of a given cell line |
| JPH04505546A (ja) | 1990-02-09 | 1992-09-24 | アスラブ ソシエテ アノニム | 写真平版マイクロ製造法を用いて製造した放射場静電マイクロモーター及び前記マイクロモーターの製造方法 |
| IE66205B1 (en) | 1990-06-14 | 1995-12-13 | Paul A Bartlett | Polypeptide analogs |
| US5650489A (en) | 1990-07-02 | 1997-07-22 | The Arizona Board Of Regents | Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof |
| US5401629A (en) | 1990-08-07 | 1995-03-28 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Assay methods and compositions useful for measuring the transduction of an intracellular signal |
| EP0578784A4 (en) | 1991-04-05 | 1994-11-02 | Univ Columbia | RECEPTORS OF SMELLING SUBSTANCES AND THEIR USES. |
| PT101031B (pt) | 1991-11-05 | 2002-07-31 | Transkaryotic Therapies Inc | Processo para o fornecimento de proteinas por terapia genetica |
| US6270989B1 (en) | 1991-11-05 | 2001-08-07 | Transkaryotic Therapies, Inc. | Protein production and delivery |
| US5641670A (en) | 1991-11-05 | 1997-06-24 | Transkaryotic Therapies, Inc. | Protein production and protein delivery |
| US5573905A (en) | 1992-03-30 | 1996-11-12 | The Scripps Research Institute | Encoded combinatorial chemical libraries |
| US5288514A (en) | 1992-09-14 | 1994-02-22 | The Regents Of The University Of California | Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support |
| US6664107B1 (en) | 1993-05-26 | 2003-12-16 | Ontario Cancer Institute, University Health Network | CD45 disrupted nucleic acid |
| US5426039A (en) | 1993-09-08 | 1995-06-20 | Bio-Rad Laboratories, Inc. | Direct molecular cloning of primer extended DNA containing an alkane diol |
| FI961251A0 (fi) * | 1993-09-20 | 1996-03-18 | Ciba Geigy | Ihmisten metabotrooppiset glutamaattireseptorialatyypit(HMR4, HMR6, HMR7)ja niihin liittyvät DNA-yhdisteet |
| IL108205A0 (en) | 1993-12-28 | 1994-04-12 | Yeda Res & Dev | Olfactory genes and receptors |
| US5519134A (en) | 1994-01-11 | 1996-05-21 | Isis Pharmaceuticals, Inc. | Pyrrolidine-containing monomers and oligomers |
| US5593853A (en) | 1994-02-09 | 1997-01-14 | Martek Corporation | Generation and screening of synthetic drug libraries |
| US5691188A (en) | 1994-02-14 | 1997-11-25 | American Cyanamid Company | Transformed yeast cells expressing heterologous G-protein coupled receptor |
| US5539083A (en) | 1994-02-23 | 1996-07-23 | Isis Pharmaceuticals, Inc. | Peptide nucleic acid combinatorial libraries and improved methods of synthesis |
| US5525735A (en) | 1994-06-22 | 1996-06-11 | Affymax Technologies Nv | Methods for synthesizing diverse collections of pyrrolidine compounds |
| US5549974A (en) | 1994-06-23 | 1996-08-27 | Affymax Technologies Nv | Methods for the solid phase synthesis of thiazolidinones, metathiazanones, and derivatives thereof |
| JPH10510433A (ja) | 1995-06-06 | 1998-10-13 | アイシス・ファーマシューティカルス・インコーポレーテッド | 高いキラル純度のホスホロチオエート結合を有するオリゴヌクレオチド |
| US5985662A (en) | 1995-07-13 | 1999-11-16 | Isis Pharmaceuticals Inc. | Antisense inhibition of hepatitis B virus replication |
| AU1118197A (en) | 1995-11-09 | 1997-05-29 | Johns Hopkins University School Of Medicine, The | Novel sperm receptors |
| US5874243A (en) | 1997-03-28 | 1999-02-23 | Smithkline Beecham Corporation | OLRCC15 receptor |
| US5993778A (en) * | 1997-05-07 | 1999-11-30 | Firestein; Stuart J. | Functional expression of, and assay for, functional cellular receptors in vivo |
| US5940153A (en) * | 1998-04-03 | 1999-08-17 | Motorola, Inc. | Display assembly having LCD and seal captured between interlocking lens cover and lightpipe |
| FR2780405B1 (fr) | 1998-06-25 | 2001-12-28 | Centre Nat Rech Scient | Nouveaux recepteurs olfactifs et leurs utilisations |
| CN1178952C (zh) * | 1998-07-28 | 2004-12-08 | 加利福尼亚大学董事会 | 编码与感觉转导有关的g-蛋白偶联受体的核酸 |
| AU4926699A (en) | 1998-07-28 | 2000-02-21 | Victoria Ann Heslop | A digester |
| IL140978A0 (en) | 1998-07-28 | 2002-02-10 | Univ California | Nucleic acids encoding proteins involved in sensory transduction |
| AU5022399A (en) | 1998-07-28 | 2000-02-21 | Canadian Inovatech, Inc. | Methods and compositions for inhibiting microbial growth in wine |
| JP2002521049A (ja) * | 1998-07-28 | 2002-07-16 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 感覚伝達に関与するｇタンパク質共役型レセプターをコードする核酸 |
| US7402400B2 (en) | 2001-07-03 | 2008-07-22 | Regents Of The University Of California | Mammalian sweet taste receptors |
| US7588900B2 (en) * | 2001-07-03 | 2009-09-15 | The Regents Of The University Of California | Mammalian sweet and amino acid heterodimeric taste receptors |
| US6106555A (en) | 1998-12-15 | 2000-08-22 | Av Healing Llc | Method for tissue fixation |
| AU2378600A (en) | 1998-12-23 | 2000-07-31 | Mount Sinai School Of Medicine Of The City University Of New York, The | Inhibitors of the bitter taste response |
| US6558910B2 (en) | 1999-09-10 | 2003-05-06 | The Regents Of The University Of California | SF, a novel family of taste receptors |
| AU773600B2 (en) * | 1999-09-10 | 2004-05-27 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | T2R, taste receptor family |
| US20030036089A1 (en) * | 1999-12-20 | 2003-02-20 | Applera Corporation | Isolated human G-protein coupled receptors, nucleic acid molecules encoding human GPCR proteins, and uses thereof |
| EP2143796A3 (en) * | 2000-02-29 | 2010-03-17 | Millennium Pharmaceuticals, Inc. | 1983, 52881, 2398, 45449, 50289, and 52872, G protein-coupled receptors and uses therefor |
| TW201006846A (en) | 2000-03-07 | 2010-02-16 | Senomyx Inc | T1R taste receptor and genes encidung same |
| US6608176B2 (en) | 2000-03-31 | 2003-08-19 | University Of Miami | Taste receptor for umami (monosodium glutamate) taste |
| WO2001077676A1 (en) | 2000-04-07 | 2001-10-18 | Senomyx, Inc. | T2r taste receptors and genes encoding same |
| JP2003531614A (ja) * | 2000-04-28 | 2003-10-28 | ワーナー−ランバート・カンパニー、リミテッド、ライアビリティ、カンパニー | 炭水化物化合物および他の甘味物質の感知に関連する遺伝子および配列変異 |
| DE60138808D1 (de) * | 2000-06-16 | 2009-07-09 | Incyte Corp | G-protein gekoppelte rezeptoren |
| TW201022287A (en) | 2001-01-03 | 2010-06-16 | Senomyx Inc | T1R taste receptors and genes encoding same |
| US7309577B2 (en) | 2001-03-07 | 2007-12-18 | Senomyx, Inc. | Binding assays that use the T1R1/T1R3 (umami) taste receptor to identify compounds that elicit or modulate umami taste |
| US7301009B2 (en) | 2001-06-26 | 2007-11-27 | Senomyx, Inc. | Isolated (T1R1/T1R3) umami taste receptors that respond to umami taste stimuli |
| US20080244761A1 (en) | 2001-03-07 | 2008-10-02 | Senomyx, Inc. | T1r hetero-oligomeric taste receptors and cell lines that express said receptors and use thereof for identification of taste compounds |
| US7763431B1 (en) | 2001-03-07 | 2010-07-27 | Senomyx, Inc. | Binding assays that use the T1R2/T1R3 (sweet) taste receptor to identify compounds that elicit or modulate sweet taste |
| US7368285B2 (en) | 2001-03-07 | 2008-05-06 | Senomyx, Inc. | Heteromeric umami T1R1/T1R3 taste receptors and isolated cells that express same |
| US6955887B2 (en) | 2001-03-30 | 2005-10-18 | Senomyx, Inc. | Use of T1R hetero-oligomeric taste receptor to screen for compounds that modulate taste signaling |
| DK2327985T3 (en) | 2001-06-26 | 2016-09-05 | Senomyx Inc | H1 Oligomeric T1R Taste Receptors and Cell Lines Expressing the Receptors, and Their Use to Identify Taste Compounds |
| CN100489102C (zh) | 2001-06-26 | 2009-05-20 | 塞诺米克斯公司 | T1r异源寡聚的味觉受体和表达所述受体的细胞系及其在鉴定味觉化合物中的用途 |
| US7022488B2 (en) * | 2003-02-03 | 2006-04-04 | Senomyx, Inc. | Functional coupling of T1Rs and T2Rs by Gi proteins, and cells-based assays for the identification of T1R and T2R modulators |
| WO2003025137A2 (en) | 2001-09-18 | 2003-03-27 | Irm, Llc | Sweet taste receptors |
| JP4411077B2 (ja) * | 2001-11-08 | 2010-02-10 | 北興化学工業株式会社 | トリオルガノ−モノアルコキシシランの製造方法とトリオルガノ−モノクロロシランの製造方法 |
| US7208290B2 (en) | 2001-12-14 | 2007-04-24 | Senomyx, Inc. | Methods of co-expressing umami taste receptors and chimeric Gα15 variants |
| US7107837B2 (en) * | 2002-01-22 | 2006-09-19 | Baxter International Inc. | Capacitance fluid volume measurement |
| US7441104B2 (en) | 2002-03-30 | 2008-10-21 | Hewlett-Packard Development Company, L.P. | Parallel subword instructions with distributed results |
| US20070142748A1 (en) * | 2002-04-19 | 2007-06-21 | Ajay Deshmukh | Tissue penetration device |
| US9314194B2 (en) * | 2002-04-19 | 2016-04-19 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
| JP2006515157A (ja) | 2002-07-29 | 2006-05-25 | セノミックス、インコーポレイテッド | 新規な苦味受容体ｔ２ｒ７６の同定 |
| NZ545747A (en) | 2003-08-06 | 2010-06-25 | Senomyx Inc | T1R hetero-oligomeric taste receptors, cell lines that express said receptors, and taste compounds |
| US7906627B2 (en) | 2003-08-06 | 2011-03-15 | Senomyx, Inc. | Chimeric human sweet-umami and umami-sweet taste receptors |
| US20050106571A1 (en) * | 2003-10-02 | 2005-05-19 | The Regents Of The University Of California | Mammalian T1R3 sweet taste receptors |
| EP1680014A4 (en) * | 2003-10-14 | 2009-01-21 | Pelikan Technologies Inc | METHOD AND APPARATUS PROVIDING A VARIABLE USER INTERFACE |
| EP1737345A1 (en) * | 2004-04-15 | 2007-01-03 | Roche Diagnostics GmbH | Integrated spot monitoring device with fluid sensor |
| US20060045953A1 (en) | 2004-08-06 | 2006-03-02 | Catherine Tachdjian | Aromatic amides and ureas and their uses as sweet and/or umami flavor modifiers, tastants and taste enhancers |
| TWI399228B (zh) * | 2006-07-21 | 2013-06-21 | Alcon Inc | 低黏性的眼科與耳鼻喉科裝置材料（二） |
| US20080047764A1 (en) * | 2006-08-28 | 2008-02-28 | Cypress Semiconductor Corporation | Temperature compensation method for capacitive sensors |
| US20090061456A1 (en) | 2007-08-30 | 2009-03-05 | Allard William J | Method for predicting progression free and overall survival at each follow-up time point during therapy of metastatic breast cancer patients using circulating tumor cells |
| US7936111B2 (en) | 2008-08-07 | 2011-05-03 | Samsung Electronics Co., Ltd. | Apparatus for generating electrical energy and method for manufacturing the same |
| JO3421B1 (ar) | 2011-06-20 | 2019-10-20 | H Lundbeck As | طريقة لإعطاء 1-((1ار.3س)-6-كلورو-3-فينيل-اندان-1-ايل)-1.2.2-ترايميثيل- بيبيرازين واملاجها لمعالجة انفصام الشخصية |
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