ES2290487T3 - Deteccion de moleculas biologicas por segmentacion diferencial de sustratos y productos enzimaticos. - Google Patents
Deteccion de moleculas biologicas por segmentacion diferencial de sustratos y productos enzimaticos. Download PDFInfo
- Publication number
- ES2290487T3 ES2290487T3 ES03747780T ES03747780T ES2290487T3 ES 2290487 T3 ES2290487 T3 ES 2290487T3 ES 03747780 T ES03747780 T ES 03747780T ES 03747780 T ES03747780 T ES 03747780T ES 2290487 T3 ES2290487 T3 ES 2290487T3
- Authority
- ES
- Spain
- Prior art keywords
- substrate
- enzyme
- sample
- fluorescence
- segmentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000758 substrate Substances 0.000 title claims abstract description 212
- 230000011218 segmentation Effects 0.000 title claims description 138
- 238000001514 detection method Methods 0.000 title claims description 111
- 230000002255 enzymatic effect Effects 0.000 title description 47
- 239000000523 sample Substances 0.000 claims abstract description 246
- 230000003287 optical effect Effects 0.000 claims abstract description 148
- 102000004190 Enzymes Human genes 0.000 claims abstract description 124
- 108090000790 Enzymes Proteins 0.000 claims abstract description 124
- 238000000034 method Methods 0.000 claims abstract description 66
- 238000006243 chemical reaction Methods 0.000 claims abstract description 51
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 50
- 244000005700 microbiome Species 0.000 claims abstract description 31
- 235000013305 food Nutrition 0.000 claims abstract description 19
- 239000012472 biological sample Substances 0.000 claims abstract description 11
- 241000588724 Escherichia coli Species 0.000 claims description 56
- 239000000835 fiber Substances 0.000 claims description 51
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 41
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 41
- 239000000356 contaminant Substances 0.000 claims description 37
- 230000005284 excitation Effects 0.000 claims description 25
- -1 polydimethylsiloxane Polymers 0.000 claims description 25
- MWPLVEDNUUSJAV-UHFFFAOYSA-N anthracene Chemical compound C1=CC=CC2=CC3=CC=CC=C3C=C21 MWPLVEDNUUSJAV-UHFFFAOYSA-N 0.000 claims description 22
- 102000053187 Glucuronidase Human genes 0.000 claims description 21
- 108010060309 Glucuronidase Proteins 0.000 claims description 21
- 241000894007 species Species 0.000 claims description 21
- 108010005774 beta-Galactosidase Proteins 0.000 claims description 18
- 230000008859 change Effects 0.000 claims description 17
- BBEAQIROQSPTKN-UHFFFAOYSA-N pyrene Chemical compound C1=CC=C2C=CC3=CC=CC4=CC=C1C2=C43 BBEAQIROQSPTKN-UHFFFAOYSA-N 0.000 claims description 17
- 239000007787 solid Substances 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 13
- 102000005936 beta-Galactosidase Human genes 0.000 claims description 13
- 239000000126 substance Substances 0.000 claims description 9
- 238000004891 communication Methods 0.000 claims description 7
- 238000001917 fluorescence detection Methods 0.000 claims description 7
- 238000011002 quantification Methods 0.000 claims description 5
- 241000700605 Viruses Species 0.000 claims description 4
- 229920001600 hydrophobic polymer Polymers 0.000 claims 12
- 230000008685 targeting Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 36
- 238000011109 contamination Methods 0.000 abstract description 7
- 239000002689 soil Substances 0.000 abstract description 7
- 238000012544 monitoring process Methods 0.000 abstract description 3
- 238000000638 solvent extraction Methods 0.000 abstract 4
- 239000000047 product Substances 0.000 description 93
- 238000012360 testing method Methods 0.000 description 68
- 210000004027 cell Anatomy 0.000 description 56
- 239000000243 solution Substances 0.000 description 55
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 42
- BIJNHUAPTJVVNQ-UHFFFAOYSA-N 1-Hydroxypyrene Chemical compound C1=C2C(O)=CC=C(C=C3)C2=C2C3=CC=CC2=C1 BIJNHUAPTJVVNQ-UHFFFAOYSA-N 0.000 description 27
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 27
- 238000003018 immunoassay Methods 0.000 description 27
- 239000000463 material Substances 0.000 description 26
- 150000001875 compounds Chemical class 0.000 description 19
- 229920000642 polymer Polymers 0.000 description 19
- 239000000203 mixture Substances 0.000 description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 16
- 229920001296 polysiloxane Polymers 0.000 description 15
- 238000003556 assay Methods 0.000 description 14
- 239000002609 medium Substances 0.000 description 14
- 238000002360 preparation method Methods 0.000 description 13
- 244000052769 pathogen Species 0.000 description 12
- BQBWUVWMUXGILF-UHFFFAOYSA-N 2-anthrol Chemical compound C1=CC=CC2=CC3=CC(O)=CC=C3C=C21 BQBWUVWMUXGILF-UHFFFAOYSA-N 0.000 description 11
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 11
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- 238000011534 incubation Methods 0.000 description 10
- 239000013307 optical fiber Substances 0.000 description 10
- 239000002904 solvent Substances 0.000 description 10
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 9
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 9
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 9
- 229930182480 glucuronide Natural products 0.000 description 9
- 239000002243 precursor Substances 0.000 description 9
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 8
- 239000003550 marker Substances 0.000 description 8
- 238000005070 sampling Methods 0.000 description 8
- 108010093031 Galactosidases Proteins 0.000 description 7
- 102000002464 Galactosidases Human genes 0.000 description 7
- 150000008134 glucuronides Chemical class 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 230000001717 pathogenic effect Effects 0.000 description 7
- 230000004044 response Effects 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- WQDUMFSSJAZKTM-UHFFFAOYSA-N Sodium methoxide Chemical compound [Na+].[O-]C WQDUMFSSJAZKTM-UHFFFAOYSA-N 0.000 description 6
- 230000002860 competitive effect Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 238000009472 formulation Methods 0.000 description 6
- 239000000499 gel Substances 0.000 description 6
- 230000012010 growth Effects 0.000 description 6
- 230000002209 hydrophobic effect Effects 0.000 description 6
- 239000012528 membrane Substances 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 5
- 238000011088 calibration curve Methods 0.000 description 5
- 239000011248 coating agent Substances 0.000 description 5
- 238000000576 coating method Methods 0.000 description 5
- 230000001276 controlling effect Effects 0.000 description 5
- 238000004132 cross linking Methods 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 238000009826 distribution Methods 0.000 description 5
- 239000000975 dye Substances 0.000 description 5
- 229920001971 elastomer Polymers 0.000 description 5
- 239000000806 elastomer Substances 0.000 description 5
- 150000008195 galaktosides Chemical class 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 239000011550 stock solution Substances 0.000 description 5
- 230000000007 visual effect Effects 0.000 description 5
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 4
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 238000013019 agitation Methods 0.000 description 4
- WQZGKKKJIJFFOK-FPRJBGLDSA-N beta-D-galactose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-FPRJBGLDSA-N 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 239000007795 chemical reaction product Substances 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000000460 chlorine Substances 0.000 description 4
- 229910052801 chlorine Inorganic materials 0.000 description 4
- 239000003344 environmental pollutant Substances 0.000 description 4
- 229960005309 estradiol Drugs 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 239000007850 fluorescent dye Substances 0.000 description 4
- UQEAIHBTYFGYIE-UHFFFAOYSA-N hexamethyldisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)C UQEAIHBTYFGYIE-UHFFFAOYSA-N 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 238000005304 joining Methods 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 4
- 231100000719 pollutant Toxicity 0.000 description 4
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 4
- MUVQKFGNPGZBII-UHFFFAOYSA-N 1-anthrol Chemical compound C1=CC=C2C=C3C(O)=CC=CC3=CC2=C1 MUVQKFGNPGZBII-UHFFFAOYSA-N 0.000 description 3
- BVTJGGGYKAMDBN-UHFFFAOYSA-N Dioxetane Chemical compound C1COO1 BVTJGGGYKAMDBN-UHFFFAOYSA-N 0.000 description 3
- 241001522878 Escherichia coli B Species 0.000 description 3
- 241000224467 Giardia intestinalis Species 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 3
- 230000002411 adverse Effects 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 229940085435 giardia lamblia Drugs 0.000 description 3
- 230000013595 glycosylation Effects 0.000 description 3
- 238000006206 glycosylation reaction Methods 0.000 description 3
- 238000011005 laboratory method Methods 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 229920001778 nylon Polymers 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 239000000741 silica gel Substances 0.000 description 3
- 229910002027 silica gel Inorganic materials 0.000 description 3
- 239000000377 silicon dioxide Substances 0.000 description 3
- ORHBXUUXSCNDEV-UHFFFAOYSA-N umbelliferone Chemical compound C1=CC(=O)OC2=CC(O)=CC=C21 ORHBXUUXSCNDEV-UHFFFAOYSA-N 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- CYAYKKUWALRRPA-UHFFFAOYSA-N (3,4,5-triacetyloxy-6-bromooxan-2-yl)methyl acetate Chemical compound CC(=O)OCC1OC(Br)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O CYAYKKUWALRRPA-UHFFFAOYSA-N 0.000 description 2
- HSHNITRMYYLLCV-UHFFFAOYSA-N 4-methylumbelliferone Chemical compound C1=C(O)C=CC2=C1OC(=O)C=C2C HSHNITRMYYLLCV-UHFFFAOYSA-N 0.000 description 2
- CJIJXIFQYOPWTF-UHFFFAOYSA-N 7-hydroxycoumarin Natural products O1C(=O)C=CC2=CC(O)=CC=C21 CJIJXIFQYOPWTF-UHFFFAOYSA-N 0.000 description 2
- KZMGYPLQYOPHEL-UHFFFAOYSA-N Boron trifluoride etherate Chemical compound FB(F)F.CCOCC KZMGYPLQYOPHEL-UHFFFAOYSA-N 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 239000004952 Polyamide Substances 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000000721 bacterilogical effect Effects 0.000 description 2
- 238000005415 bioluminescence Methods 0.000 description 2
- 230000029918 bioluminescence Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- IJOOHPMOJXWVHK-UHFFFAOYSA-N chlorotrimethylsilane Chemical compound C[Si](C)(C)Cl IJOOHPMOJXWVHK-UHFFFAOYSA-N 0.000 description 2
- 239000003593 chromogenic compound Substances 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- XDMMMGGRRNTWML-UHFFFAOYSA-N cyclohexylazanium;acetate Chemical compound CC(O)=O.NC1CCCCC1 XDMMMGGRRNTWML-UHFFFAOYSA-N 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000014155 detection of activity Effects 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 239000003651 drinking water Substances 0.000 description 2
- 235000020188 drinking water Nutrition 0.000 description 2
- 238000003028 enzyme activity measurement method Methods 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 230000002550 fecal effect Effects 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- PCKPVGOLPKLUHR-UHFFFAOYSA-N indoxyl Chemical group C1=CC=C2C(O)=CNC2=C1 PCKPVGOLPKLUHR-UHFFFAOYSA-N 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000000704 physical effect Effects 0.000 description 2
- 229920002647 polyamide Polymers 0.000 description 2
- AOJFQRQNPXYVLM-UHFFFAOYSA-N pyridin-1-ium;chloride Chemical compound [Cl-].C1=CC=[NH+]C=C1 AOJFQRQNPXYVLM-UHFFFAOYSA-N 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 159000000000 sodium salts Chemical class 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- HFTAFOQKODTIJY-UHFFFAOYSA-N umbelliferone Natural products Cc1cc2C=CC(=O)Oc2cc1OCC=CC(C)(C)O HFTAFOQKODTIJY-UHFFFAOYSA-N 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 229910052724 xenon Inorganic materials 0.000 description 2
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 2
- HRYLCRMCPOGATF-YHUYYLMFSA-N (4s,5s,6s,7r)-4,5,6-triacetyl-4,5,6,7-tetrahydroxy-7-(hydroxymethyl)nonane-2,3,8-trione Chemical compound CC(=O)C(=O)[C@@](O)(C(C)=O)[C@](O)(C(C)=O)[C@](O)(C(C)=O)[C@](O)(CO)C(C)=O HRYLCRMCPOGATF-YHUYYLMFSA-N 0.000 description 1
- CWRWJDAEKWYUJT-CGKXPTHNSA-N 1-(9S,13S,12-oxophytodienoyl)-2-(7Z,10Z,13Z)-hexadecatrienoyl-3-(beta-D-galactosyl)-sn-glycerol Chemical compound C([C@H](OC(=O)CCCCC\C=C/C\C=C/C\C=C/CC)COC(=O)CCCCCCC[C@@H]1[C@@H](C(=O)C=C1)C\C=C/CC)O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O CWRWJDAEKWYUJT-CGKXPTHNSA-N 0.000 description 1
- ZQXCQTAELHSNAT-UHFFFAOYSA-N 1-chloro-3-nitro-5-(trifluoromethyl)benzene Chemical compound [O-][N+](=O)C1=CC(Cl)=CC(C(F)(F)F)=C1 ZQXCQTAELHSNAT-UHFFFAOYSA-N 0.000 description 1
- UPQQXPKAYZYUKO-UHFFFAOYSA-N 2,2,2-trichloroacetamide Chemical compound OC(=N)C(Cl)(Cl)Cl UPQQXPKAYZYUKO-UHFFFAOYSA-N 0.000 description 1
- HIXDQWDOVZUNNA-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-hydroxy-7-methoxychromen-4-one Chemical compound C=1C(OC)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(OC)C(OC)=C1 HIXDQWDOVZUNNA-UHFFFAOYSA-N 0.000 description 1
- BDKLKNJTMLIAFE-UHFFFAOYSA-N 2-(3-fluorophenyl)-1,3-oxazole-4-carbaldehyde Chemical compound FC1=CC=CC(C=2OC=C(C=O)N=2)=C1 BDKLKNJTMLIAFE-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- MJKVTPMWOKAVMS-UHFFFAOYSA-N 3-hydroxy-1-benzopyran-2-one Chemical class C1=CC=C2OC(=O)C(O)=CC2=C1 MJKVTPMWOKAVMS-UHFFFAOYSA-N 0.000 description 1
- XZKIHKMTEMTJQX-UHFFFAOYSA-N 4-Nitrophenyl Phosphate Chemical compound OP(O)(=O)OC1=CC=C([N+]([O-])=O)C=C1 XZKIHKMTEMTJQX-UHFFFAOYSA-N 0.000 description 1
- BTJIUGUIPKRLHP-UHFFFAOYSA-N 4-nitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-N 0.000 description 1
- TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 description 1
- PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000588919 Citrobacter freundii Species 0.000 description 1
- 241000588697 Enterobacter cloacae Species 0.000 description 1
- 241001646719 Escherichia coli O157:H7 Species 0.000 description 1
- 241000588747 Klebsiella pneumoniae Species 0.000 description 1
- 239000006137 Luria-Bertani broth Substances 0.000 description 1
- 241000714209 Norwalk virus Species 0.000 description 1
- 208000012868 Overgrowth Diseases 0.000 description 1
- 241000315672 SARS coronavirus Species 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- 239000012131 assay buffer Substances 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 238000009227 behaviour therapy Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000003131 biological toxin Substances 0.000 description 1
- 239000012490 blank solution Substances 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000013626 chemical specie Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000003653 coastal water Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000002967 competitive immunoassay Methods 0.000 description 1
- 229940126214 compound 3 Drugs 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000012084 conversion product Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 150000004775 coumarins Chemical class 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- PAFZNILMFXTMIY-UHFFFAOYSA-N cyclohexylamine Chemical class NC1CCCCC1 PAFZNILMFXTMIY-UHFFFAOYSA-N 0.000 description 1
- PAFZNILMFXTMIY-UHFFFAOYSA-O cyclohexylammonium Chemical compound [NH3+]C1CCCCC1 PAFZNILMFXTMIY-UHFFFAOYSA-O 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010511 deprotection reaction Methods 0.000 description 1
- 230000014670 detection of bacterium Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 231100000613 environmental toxicology Toxicity 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 1
- 125000001033 ether group Chemical group 0.000 description 1
- CEIPQQODRKXDSB-UHFFFAOYSA-N ethyl 3-(6-hydroxynaphthalen-2-yl)-1H-indazole-5-carboximidate dihydrochloride Chemical compound Cl.Cl.C1=C(O)C=CC2=CC(C3=NNC4=CC=C(C=C43)C(=N)OCC)=CC=C21 CEIPQQODRKXDSB-UHFFFAOYSA-N 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- GVEPBJHOBDJJJI-UHFFFAOYSA-N fluoranthrene Natural products C1=CC(C2=CC=CC=C22)=C3C2=CC=CC3=C1 GVEPBJHOBDJJJI-UHFFFAOYSA-N 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical class O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000002189 fluorescence spectrum Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 125000001041 indolyl group Chemical group 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000012933 kinetic analysis Methods 0.000 description 1
- 229910052747 lanthanoid Inorganic materials 0.000 description 1
- 150000002602 lanthanoids Chemical class 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- UPSFMJHZUCSEHU-JYGUBCOQSA-N n-[(2s,3r,4r,5s,6r)-2-[(2r,3s,4r,5r,6s)-5-acetamido-4-hydroxy-2-(hydroxymethyl)-6-(4-methyl-2-oxochromen-7-yl)oxyoxan-3-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide Chemical compound CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]1[C@H](O)[C@@H](NC(C)=O)[C@H](OC=2C=C3OC(=O)C=C(C)C3=CC=2)O[C@@H]1CO UPSFMJHZUCSEHU-JYGUBCOQSA-N 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000005693 optoelectronics Effects 0.000 description 1
- 150000002902 organometallic compounds Chemical class 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 102000013415 peroxidase activity proteins Human genes 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 229940085991 phosphate ion Drugs 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 150000003071 polychlorinated biphenyls Chemical class 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 238000013102 re-test Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical class [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 229910052594 sapphire Inorganic materials 0.000 description 1
- 239000010980 sapphire Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 229940087562 sodium acetate trihydrate Drugs 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
- 235000019345 sodium thiosulphate Nutrition 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000000935 solvent evaporation Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- DZLFLBLQUQXARW-UHFFFAOYSA-N tetrabutylammonium Chemical compound CCCC[N+](CCCC)(CCCC)CCCC DZLFLBLQUQXARW-UHFFFAOYSA-N 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56905—Protozoa
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/808—Optical sensing apparatus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Toxicology (AREA)
- Optics & Photonics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41201502P | 2002-09-20 | 2002-09-20 | |
| US412015P | 2002-09-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2290487T3 true ES2290487T3 (es) | 2008-02-16 |
Family
ID=32030780
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES03747780T Expired - Lifetime ES2290487T3 (es) | 2002-09-20 | 2003-09-22 | Deteccion de moleculas biologicas por segmentacion diferencial de sustratos y productos enzimaticos. |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US7402426B2 (enExample) |
| EP (1) | EP1539986B1 (enExample) |
| JP (2) | JP4857434B2 (enExample) |
| AT (1) | ATE367449T1 (enExample) |
| AU (1) | AU2003266899B2 (enExample) |
| CA (1) | CA2497555C (enExample) |
| DE (1) | DE60315043T2 (enExample) |
| ES (1) | ES2290487T3 (enExample) |
| MX (1) | MXPA05003068A (enExample) |
| WO (1) | WO2004027084A1 (enExample) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003266899B2 (en) | 2002-09-20 | 2008-02-28 | Queen's University At Kingston | Detection of biological molecules by differential partitioning of enzyme substrates and products |
| US7964390B2 (en) * | 2002-10-11 | 2011-06-21 | Case Western Reserve University | Sensor system |
| US20070026426A1 (en) | 2005-04-26 | 2007-02-01 | Applera Corporation | System for genetic surveillance and analysis |
| WO2007035763A2 (en) * | 2005-09-20 | 2007-03-29 | The Arizona Board Of Regents, A Body Corporate Acting On Behalf Of Arizona State University | Methods for detecting and characterizing microorganisms |
| EP1984756A2 (en) * | 2006-02-13 | 2008-10-29 | Thermo Electron Scientific Instruments LLC | Spectrometric measurements during blending/mixing |
| JP5374809B2 (ja) * | 2006-06-29 | 2013-12-25 | パナソニック株式会社 | 微生物計測システム |
| US10213164B2 (en) | 2008-09-26 | 2019-02-26 | Qualcomm Incorporated | Method and apparatus for under-sampled acquisition and transmission of photoplethysmograph (PPG) data and reconstruction of full band PPG data at the receiver |
| EP2404162B1 (en) | 2009-03-03 | 2017-12-27 | Access Medical System Co., Ltd. | Detection system and method for high sensitivity fluorescent assays |
| AU2010248902B2 (en) * | 2009-05-15 | 2015-02-26 | Biomerieux, Inc. | Automated microbial detection apparatus |
| CN102803959B (zh) | 2009-05-15 | 2015-07-08 | 生物梅里埃有限公司 | 用于样品中的微生物剂的快速的识别和/或表征的系统和方法 |
| US8891086B2 (en) * | 2009-06-15 | 2014-11-18 | Thermo Scientific Portable Analytical Instruments Inc. | Optical scanning systems and methods for measuring a sealed container with a layer for reducing diffusive scattering |
| FR2949560A1 (fr) * | 2009-09-02 | 2011-03-04 | Centre Nat Rech Scient | Systeme de spectroscopie a guide d'onde pour l'analyse de particules dans un milieu |
| CN102686739B (zh) * | 2009-12-22 | 2014-09-24 | 3M创新有限公司 | 检测微生物的方法和其套件 |
| US9097672B2 (en) | 2010-06-18 | 2015-08-04 | Queens's University At Kingston | Method and system for detecting biological molecules in samples |
| EP2582784A4 (en) * | 2010-06-18 | 2014-01-01 | Pathogen Detection Systems Inc | OPTICAL DISEASE WELDING SYSTEM AND QUALITY CONTROL MATERIALS FOR USE THEREIN |
| JP5621846B2 (ja) * | 2010-06-21 | 2014-11-12 | コニカミノルタ株式会社 | 蛍光測定方法 |
| CN103189524B (zh) | 2010-11-01 | 2015-01-28 | 3M创新有限公司 | 生物灭菌指示器及其使用方法 |
| ES2687181T3 (es) | 2010-11-01 | 2018-10-24 | 3M Innovative Properties Company | Método para detectar una actividad biológica |
| JP2014503196A (ja) * | 2010-11-24 | 2014-02-13 | スリーエム イノベイティブ プロパティズ カンパニー | 生物活性を検出する方法及び組成物 |
| EP2768906B1 (en) | 2011-10-18 | 2019-02-27 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Fluorescent dyes with phosphorylated hydroxymethyl groups and their use in light microscopy and imaging techniques |
| KR101343353B1 (ko) | 2012-05-04 | 2013-12-20 | 한국과학기술연구원 | 엔테로박터 및 에스케리키아의 구별 방법 |
| US9055992B2 (en) * | 2012-12-14 | 2015-06-16 | Bryan Larson | Dual medicament carpule for dental syringes |
| EP2954102B1 (en) * | 2013-02-08 | 2018-12-19 | Bio-Rad Laboratories, Inc. | Affinity-based partition assay for detection of target molecules |
| CN104034705B (zh) * | 2013-03-06 | 2017-05-10 | 常州欣宏科生物化学有限公司 | 一种荧光探针检测酶活性方法 |
| JP6567274B2 (ja) * | 2014-12-10 | 2019-08-28 | 水ｉｎｇ株式会社 | 分離膜の汚染状態分析方法、その方法を用いるろ過対象水の水質評価方法 |
| US11656180B2 (en) | 2015-08-03 | 2023-05-23 | Ysi, Inc. | Multi excitation-multi emission fluorometer for multiparameter water quality monitoring |
| EP3332243B1 (en) * | 2015-08-03 | 2024-05-01 | YSI, Inc. | Multi excitation-multi emission fluorometer for multiparameter water quality monitoring |
| AU2019279025A1 (en) * | 2018-06-01 | 2021-01-07 | Orb Xyz, Inc. | Detecting an analyte in a medium |
| JP6935883B2 (ja) * | 2018-07-13 | 2021-09-15 | 五稜化薬株式会社 | 蛍光画像診断の前処理方法 |
| CN110763823B (zh) * | 2019-11-22 | 2022-04-12 | 威海精讯畅通电子科技有限公司 | 一种手持式土壤快速检测仪及检测方法 |
| CN114428070B (zh) * | 2021-12-28 | 2024-05-17 | 南京师范大学 | 一种检测革兰氏阴性菌的分子印迹荧光纳米粒子及其制备方法 |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2856251A1 (de) * | 1978-12-27 | 1980-07-17 | Max Planck Gesellschaft | Fuellungen fuer indikatorraeume |
| DK256581A (da) * | 1980-06-13 | 1981-12-14 | Nat Res Dev | Bindingsanalyse |
| GB2078370A (en) | 1980-06-13 | 1982-01-06 | Nat Res Dev | Binding assays |
| AT377095B (de) * | 1982-11-23 | 1985-02-11 | List Hans | Sensorelement zur bestimmung des o2-gehaltes einer probe sowie verfahren zur herstellung desselben |
| US5238809A (en) * | 1986-02-03 | 1993-08-24 | Avl Medical Instruments Ag | Method for optical determination of the catalytic enzyme activity and arrangement for implementing this method |
| AT392539B (de) * | 1986-02-03 | 1991-04-25 | Avl Verbrennungskraft Messtech | Verfahren zur optischen bestimmung der katalytischen enzymaktivitaet einer probe, und eine anordnung zur durchfuehrung des verfahrens |
| JPS62203047A (ja) * | 1986-02-28 | 1987-09-07 | Advance:Kk | アンモニアセンサ− |
| US4712865A (en) * | 1987-01-05 | 1987-12-15 | Baxter Travenol Laboratories | Dye containing silicon polymer composition |
| JPH0814541B2 (ja) * | 1987-04-03 | 1996-02-14 | ティーディーケイ株式会社 | 光フアイバ−センサ− |
| JPS6463842A (en) * | 1987-09-03 | 1989-03-09 | Terumo Corp | Method and apparatus for measuring concentration of optical material |
| US4974929A (en) * | 1987-09-22 | 1990-12-04 | Baxter International, Inc. | Fiber optical probe connector for physiologic measurement devices |
| FR2650076B1 (fr) * | 1989-07-20 | 1991-10-04 | Commissariat Energie Atomique | Capteur chimique actif a fibre optique et son procede de fabrication |
| JP3065639B2 (ja) | 1990-08-22 | 2000-07-17 | 三菱電線工業株式会社 | ケーブル事故検出装置 |
| US5376551A (en) * | 1991-03-12 | 1994-12-27 | University Of Utah Research Foundation | Apparatus for using fluorescently labeled ligands in studying interaction of a native ligand and its receptor |
| WO1992016648A1 (en) * | 1991-03-18 | 1992-10-01 | Environmental Test Systems, Inc. | Chemiluminescent test for microorganisms |
| JP3048672B2 (ja) * | 1991-04-30 | 2000-06-05 | 栄研化学株式会社 | 試薬結合ポリマー、該ポリマー膜および該ポリマーを含む担持体 |
| GB9116909D0 (en) | 1991-08-06 | 1991-09-18 | Salford Ultrafine Chem & Res | Morphine derivatives |
| GB9215973D0 (en) * | 1992-07-28 | 1992-09-09 | Univ Manchester | Sensor devices |
| US5364767A (en) | 1993-02-11 | 1994-11-15 | Research Organics, In. | Chromogenic compounds and methods of using same |
| GB9315183D0 (en) * | 1993-07-22 | 1993-09-08 | British Nuclear Fuels Plc | Biosensors |
| JPH07147997A (ja) * | 1993-11-26 | 1995-06-13 | Apere:Kk | 微生物検査方法および微生物検査装置 |
| US5861270A (en) * | 1994-11-07 | 1999-01-19 | Universiteit Gent | Enzymatic method for detecting coliform bacteria or E. coli |
| EP0971031A2 (en) | 1995-09-29 | 2000-01-12 | Idexx Laboratories, Inc. | Container for growth of microorganism |
| DE69633573T2 (de) * | 1995-11-22 | 2005-10-06 | Medtronic MiniMed, Inc., Northridge | Detektion von biologischen molekülen unter verwendung von chemischer amplifikation und optischem sensor |
| JPH09152433A (ja) * | 1995-11-30 | 1997-06-10 | Aisin Seiki Co Ltd | 固相担体に固定した核酸等の検出方法 |
| US5972595A (en) * | 1997-12-19 | 1999-10-26 | Nen Life Science Products, Inc. | Enzyme assay using a solid phase substrate |
| US6372895B1 (en) * | 2000-07-07 | 2002-04-16 | 3M Innovative Properties Company | Fluorogenic compounds |
| US7662612B2 (en) * | 2000-05-11 | 2010-02-16 | Panasonic Corporation | Chemical sensor device |
| AU2002250295A1 (en) | 2001-03-16 | 2002-10-03 | Ewatertek Inc. | System and method for monitoring water quality and transmitting water quality data |
| US7096053B2 (en) * | 2001-10-02 | 2006-08-22 | Alfred E. Mann Institute For Biomedical Engineering At The University Of Southern California | Internal biochemical sensing device |
| WO2003049840A1 (en) * | 2001-12-06 | 2003-06-19 | Purdue Research Foundation | Mesoporous membrane collector and separator for airborne pathogen detection |
| US20030228681A1 (en) * | 2002-04-05 | 2003-12-11 | Powerzyme, Inc. | Analyte sensor |
| US20040047535A1 (en) * | 2002-09-09 | 2004-03-11 | Ljerka Ukrainczyk | Enhanced fiber-optic sensor |
| AU2003266899B2 (en) | 2002-09-20 | 2008-02-28 | Queen's University At Kingston | Detection of biological molecules by differential partitioning of enzyme substrates and products |
-
2003
- 2003-09-22 AU AU2003266899A patent/AU2003266899B2/en not_active Expired
- 2003-09-22 AT AT03747780T patent/ATE367449T1/de active
- 2003-09-22 WO PCT/CA2003/001439 patent/WO2004027084A1/en not_active Ceased
- 2003-09-22 DE DE60315043T patent/DE60315043T2/de not_active Expired - Lifetime
- 2003-09-22 JP JP2004536735A patent/JP4857434B2/ja not_active Expired - Lifetime
- 2003-09-22 US US10/665,718 patent/US7402426B2/en not_active Expired - Lifetime
- 2003-09-22 ES ES03747780T patent/ES2290487T3/es not_active Expired - Lifetime
- 2003-09-22 CA CA2497555A patent/CA2497555C/en not_active Expired - Lifetime
- 2003-09-22 EP EP03747780A patent/EP1539986B1/en not_active Expired - Lifetime
- 2003-09-22 MX MXPA05003068A patent/MXPA05003068A/es active IP Right Grant
-
2008
- 2008-06-16 US US12/213,207 patent/US8377686B2/en not_active Expired - Fee Related
-
2011
- 2011-06-13 JP JP2011130892A patent/JP5799353B2/ja not_active Expired - Lifetime
-
2013
- 2013-01-10 US US13/738,620 patent/US8632966B2/en not_active Expired - Lifetime
- 2013-11-18 US US14/082,376 patent/US20140295403A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1539986B1 (en) | 2007-07-18 |
| DE60315043D1 (de) | 2007-08-30 |
| CA2497555A1 (en) | 2004-04-01 |
| WO2004027084A1 (en) | 2004-04-01 |
| HK1080514A1 (en) | 2006-04-28 |
| US20140295403A1 (en) | 2014-10-02 |
| JP2011254816A (ja) | 2011-12-22 |
| AU2003266899B2 (en) | 2008-02-28 |
| DE60315043T2 (de) | 2008-04-10 |
| US20090117600A1 (en) | 2009-05-07 |
| US20130217041A1 (en) | 2013-08-22 |
| US8377686B2 (en) | 2013-02-19 |
| CA2497555C (en) | 2013-06-11 |
| MXPA05003068A (es) | 2005-06-08 |
| JP4857434B2 (ja) | 2012-01-18 |
| JP5799353B2 (ja) | 2015-10-21 |
| JP2005538728A (ja) | 2005-12-22 |
| ATE367449T1 (de) | 2007-08-15 |
| US8632966B2 (en) | 2014-01-21 |
| US20040106164A1 (en) | 2004-06-03 |
| EP1539986A1 (en) | 2005-06-15 |
| AU2003266899A1 (en) | 2004-04-08 |
| US7402426B2 (en) | 2008-07-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2290487T3 (es) | Deteccion de moleculas biologicas por segmentacion diferencial de sustratos y productos enzimaticos. | |
| KR970003150B1 (ko) | 미생물 검출 장치 및 기기 | |
| JP3010565B2 (ja) | 微生物検出用装置および方法 | |
| EP0538450A1 (en) | Method and apparatus to detect bacterial contamination of transfusable blood | |
| CN100565190C (zh) | 生化物质传感方法和生物传感器光学传感结构 | |
| Wang et al. | A group-targeting biosensor for sensitive and rapid detection of quinolones in water samples | |
| US8067154B2 (en) | Method and device for rapid detection of microorganisms by changing the shape of micro-colonies in micro-channels | |
| Seeley et al. | Selected Exercises for Microbes in Action | |
| Class et al. | Patent application title: DETECTION OF BIOLOGICAL MOLECULES BY DIFFERENTIAL PARTITIONING OF ENZYME SUBSTRATES AND PRODUCTS | |
| JPH05504263A (ja) | 吸光度に制御された蛍光の変化をモニターすることによる隔離された蛍光団中における細菌性の二酸化炭素産生の測定 | |
| HK1080514B (en) | Detection of biological molecules by differential partitioning of enzyme substrates and products | |
| GB2261729A (en) | Method and optical probe for the determination of reduced nicotinamide adenine dinucleotide in a sample | |
| Sheng | A Novel Fiber-optic Probe for Detection of Enzyme Activity Using Conventional Substrates | |
| Pang | Development of microplate-based biosensors for high-throughput measurements | |
| Ligler et al. | OPTRODE-BASED FIBER OPTIC BIOSENSORS | |
| CN104975065A (zh) | 一种基于生化初筛及荧光纳米生物探针检测沙门菌的方法 |