ES2224908T1 - Modulacion de la degradacion de la urea en levaduras vinicas. - Google Patents
Modulacion de la degradacion de la urea en levaduras vinicas.Info
- Publication number
- ES2224908T1 ES2224908T1 ES02771969T ES02771969T ES2224908T1 ES 2224908 T1 ES2224908 T1 ES 2224908T1 ES 02771969 T ES02771969 T ES 02771969T ES 02771969 T ES02771969 T ES 02771969T ES 2224908 T1 ES2224908 T1 ES 2224908T1
- Authority
- ES
- Spain
- Prior art keywords
- yeast strain
- strain
- fermented
- yeast
- coding sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 title claims abstract 7
- 239000004202 carbamide Substances 0.000 title claims abstract 7
- 230000015556 catabolic process Effects 0.000 title 1
- 238000006731 degradation reaction Methods 0.000 title 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract 22
- 238000000855 fermentation Methods 0.000 claims abstract 10
- 230000004151 fermentation Effects 0.000 claims abstract 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract 8
- 230000000694 effects Effects 0.000 claims abstract 5
- 108090000790 Enzymes Proteins 0.000 claims abstract 4
- 102000004190 Enzymes Human genes 0.000 claims abstract 4
- 230000000593 degrading effect Effects 0.000 claims abstract 4
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract 4
- 108091026890 Coding region Proteins 0.000 claims 7
- 235000013334 alcoholic beverage Nutrition 0.000 claims 5
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 3
- 102000039446 nucleic acids Human genes 0.000 claims 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 2
- JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 claims 2
- 108700026244 Open Reading Frames Proteins 0.000 claims 2
- 235000013361 beverage Nutrition 0.000 claims 2
- 239000001064 degrader Substances 0.000 claims 2
- 230000002255 enzymatic effect Effects 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 108700023205 Allophanate hydrolases Proteins 0.000 claims 1
- 101150016414 DUR1,2 gene Proteins 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 230000001476 alcoholic effect Effects 0.000 claims 1
- 150000001720 carbohydrates Chemical class 0.000 claims 1
- 235000019441 ethanol Nutrition 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- 108010010296 urea carboxylase (hydrolyzing) Proteins 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C12/00—Processes specially adapted for making special kinds of beer
- C12C12/002—Processes specially adapted for making special kinds of beer using special microorganisms
- C12C12/004—Genetically modified microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C12/00—Processes specially adapted for making special kinds of beer
- C12C12/002—Processes specially adapted for making special kinds of beer using special microorganisms
- C12C12/006—Yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G1/00—Preparation of wine or sparkling wine
- C12G1/02—Preparation of must from grapes; Must treatment and fermentation
- C12G1/0203—Preparation of must from grapes; Must treatment and fermentation by microbiological or enzymatic treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Una cepa de levadura transformada para reducir la represión por catabolito nitrogenado de una actividad enzimática degradadora de urea expresada por la cepa de levadura bajo condiciones de fermentación.
Claims (20)
1. Una cepa de levadura transformada para reducir
la represión por catabolito nitrogenado de una actividad enzimática
degradadora de urea expresada por la cepa de levadura bajo
condiciones de fermentación.
2. La cepa de levadura de la reivindicación 1, en
donde la actividad enzimática degradadora de urea es una actividad
urea carboxilasa o alofanato hidrolasa.
3. La cepa de levadura de la reivindicación 1 ó
2, en donde la cepa es transformada con un ácido nucléico
recombinante que comprende una secuencia codificante que codifica
para la actividad enzimática degradadora de urea.
4. La cepa de levadura de la reivindicación 1 ó
2, en donde la cepa es transformada con un ácido nucléico
recombinante que comprende un promotor adaptado para regular la
expresión de la actividad enzimática degradadora de urea bajo
condiciones de fermentación.
5. La cepa de levadura de la reivindicación 3, en
donde la secuencia codificante comprende un marco abierto de lectura
homólogo al marco de lectura abierto de DUR1,2.
6. La cepa de levadura de cualquiera de las
reivindicaciones 1 a 5, en donde bajo las condiciones de
fermentación la cepa de levadura fermenta un carbohidrato para
producir alcohol etílico.
7. La cepa de levadura de cualquiera de la
reivindicaciones 1 a 6, en donde la cepa de levadura es una cepa de
S. cerevisiae.
8. La cepa de levadura de cualquiera de las
reivindicaciones 1 a 7, en donde bajo las condiciones de
fermentación la cepa de levadura produce un vino que posee una
concentración de carbamato de etilo de menos de 30 ppb.
9. Una cepa de levadura substancialmente tal como
la que aquí se describe y con referencia a los ejemplos y
dibujos.
10. El uso de la cepa de levadura de cualquiera
de las reivindicaciones 1 a 9 para producir una bebida alcohólica
fermentada.
11. Un método de fabricar una bebida alcohólica
fermentada que comprende mantener la cepa de levadura de cualquiera
de las reivindicaciones 1 a 9 bajo condiciones de fermentación.
12. La bebida alcohólica fermentada de la
reivindicación 10 o de la reivindicación 11.
13. La bebida alcohólica fermentada de la
reivindicación 12, en donde la bebida es un vino o una bebida
alcohólica destilada.
14. La bebida alcohólica fermentada de la
reivindicación 13, en donde la bebida es vino, y el vino tiene una
concentración de carbamato de etilo de menos de 30 ppb.
15. El uso de la cepa de levadura de cualquiera
de las reivindicaciones 1 a 9 para producir un producto
fermentado.
16. Un método de fabricar un producto fermentado
que comprende mantener la cepa de levadura de cualquiera de las
reivindicaciones 1 a 9 bajo condiciones de fermentación.
17. El producto fermentado de la reivindicación
15 o de la reivindicación 16.
18. Un método de modificar una cepa de levadura,
que comprende transformar la cepa de levadura para reducir la
represión por catabolito nitrogenado de una actividad enzimática
degradadora de urea expresada por la cepa de levadura bajo las
condiciones de fermentación.
19. Un ácido nucléico aislado que comprende una
secuencia promotora que es 95% idéntica cuando es óptimamente
alineada a una secuencia promotora DUR1,2 nativa, en donde la
secuencia promotora no regula la represión por catabolito
nitrogenado de una secuencia codificante operativamente unida bajo
condiciones de fermentación en una célula hospedadora.
20. Una secuencia de ácido nucléico aislada que
comprende una secuencia codificante que es 80% idéntica cuando es
óptimamente alineada a una secuencia codificante de DUR1,2 nativa,
en donde la secuencia codificante está operativamente unida a una
secuencia promotora que no regula la represión por catabolito
nitrogenado de la secuencia codificante bajo condiciones de
fermentación en una célula hospedadora.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US33099301P | 2001-11-06 | 2001-11-06 | |
| US330993P | 2001-11-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2224908T1 true ES2224908T1 (es) | 2005-03-16 |
Family
ID=23292178
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES02771969T Pending ES2224908T1 (es) | 2001-11-06 | 2002-11-06 | Modulacion de la degradacion de la urea en levaduras vinicas. |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US8187859B2 (es) |
| EP (1) | EP1453982A2 (es) |
| JP (1) | JP2005508191A (es) |
| CN (1) | CN1578839B (es) |
| AU (1) | AU2002336876B2 (es) |
| CA (1) | CA2464977A1 (es) |
| CL (1) | CL2004000517A1 (es) |
| DE (1) | DE02771969T1 (es) |
| ES (1) | ES2224908T1 (es) |
| HU (1) | HUP0401860A3 (es) |
| MX (1) | MXPA04004278A (es) |
| RU (1) | RU2363733C2 (es) |
| WO (1) | WO2003040379A2 (es) |
| ZA (1) | ZA200304529B (es) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861348A (zh) * | 2016-06-14 | 2016-08-17 | 江南大学 | 一株低产尿素的酿酒酵母及其在食品生产中的应用 |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2276830A4 (en) * | 2008-04-14 | 2013-03-20 | Univ British Columbia | FUNCTIONAL IMPROVEMENT OF YEAST TO MINIMIZE ETHYL CARBAMATE PRODUCTION BY MODIFIED TRANSPORT EXPRESSION |
| CN102533573A (zh) * | 2011-11-10 | 2012-07-04 | 江南大学 | 一种低产尿素的黄酒酵母代谢工程菌及其构建方法 |
| EP2941481A4 (en) | 2013-01-04 | 2016-11-02 | Novogy Inc | MANIPULATED MICROORGANISMS FOR USE AS UNCONVENTIONAL NITROGEN SOURCES |
| CN103409330B (zh) * | 2013-04-08 | 2015-04-22 | 中国食品发酵工业研究院 | 一种低产氨基甲酸乙酯的葡萄酒酵母及应用 |
| CN103710278A (zh) * | 2013-12-25 | 2014-04-09 | 江南大学 | 一种低产尿素的工业黄酒酵母代谢工程菌及其构建方法 |
| EP3129479A4 (en) * | 2014-04-08 | 2017-10-25 | Novogy Inc. | Selective advantage in fermentation |
| WO2015168534A1 (en) * | 2014-05-02 | 2015-11-05 | Novogy, Inc. | Therapeutic treatment of gastrointestinal microbial imbalances through competitive microbe displacement |
| CN106119141A (zh) * | 2016-01-12 | 2016-11-16 | 天津科技大学 | 一株通过敲除car1过表达dur1，2低产氨基甲酸乙酯酿酒酵母菌株及其构建方法 |
| CN106119142A (zh) * | 2016-01-12 | 2016-11-16 | 天津科技大学 | 一株通过敲除car1过表达dur3低产氨基甲酸乙酯酿酒酵母菌株及其构建方法 |
| CN106635848B (zh) * | 2016-10-19 | 2019-06-07 | 江南大学 | 一株同时降解精氨酸和尿素的粟酒裂殖酵母菌 |
| KR102350289B1 (ko) * | 2019-05-07 | 2022-01-12 | 주식회사 피토맵 | 질소결핍 유도성 프로모터 및 이를 포함하는 유전자 발현 시스템 |
| CN112175764A (zh) * | 2020-10-27 | 2021-01-05 | 江南大学 | 降解氰化物的酿酒酵母在食品生产中的应用 |
| CN113444766B (zh) * | 2021-06-04 | 2024-05-24 | 海天醋业集团有限公司 | 用于发酵酒陈酿过程中变质菌的富集培养基及的检测方法 |
| CN114107362B (zh) * | 2021-11-18 | 2023-07-25 | 江南大学 | 染色质调控因子Ahc1p在减少酿酒酵母尿素积累中的应用 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4458066A (en) * | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4401796A (en) * | 1981-04-30 | 1983-08-30 | City Of Hope Research Institute | Solid-phase synthesis of polynucleotides |
| US4373071A (en) * | 1981-04-30 | 1983-02-08 | City Of Hope Research Institute | Solid-phase synthesis of polynucleotides |
| US4598049A (en) * | 1983-08-31 | 1986-07-01 | Systec Inc. | General purpose gene synthesizer |
| JPH0783706B2 (ja) * | 1986-10-14 | 1995-09-13 | 武田薬品工業株式会社 | 酒類の品質改良法 |
| JPS63226274A (ja) * | 1987-03-16 | 1988-09-20 | Hakutsuru Syuzo Kk | 清酒の製造方法 |
| JPH0771472B2 (ja) * | 1989-08-14 | 1995-08-02 | 国税庁長官 | 尿素非生産性実用醸造酵母 |
| JPH0697993B2 (ja) * | 1990-05-15 | 1994-12-07 | 国税庁長官 | アルギナーゼ遺伝子欠損株選択培地とそれを利用した尿素非生産性酵母の育種及びそれを用いる酒類の製造法 |
| JPH05244959A (ja) * | 1992-03-05 | 1993-09-24 | Toyobo Co Ltd | ウレアアミドリアーゼの遺伝情報を有するｄｎａおよびその用途 |
| EP0827541B1 (en) * | 1995-05-18 | 2009-11-18 | University of Stellenbosch | Nucleic acid sequence coding for a malate permease from s. pombe and uses thereof |
| GB9524752D0 (en) * | 1995-12-04 | 1996-02-07 | Danisco | Modification process |
| DK0785275T3 (da) * | 1996-01-16 | 2000-11-06 | Dsm Nv | Transformerede gærstammer |
| AU720988B2 (en) * | 1997-01-16 | 2000-06-22 | Regents Of The University Of California, The | Improved wine yeast cultures |
| US6159759A (en) * | 1999-11-19 | 2000-12-12 | Chartered Semiconductor Manufacturing Ltd. | Method to form liquid crystal displays using a triple damascene technique |
-
2002
- 2002-11-06 DE DE02771969T patent/DE02771969T1/de active Pending
- 2002-11-06 WO PCT/CA2002/001719 patent/WO2003040379A2/en active Application Filing
- 2002-11-06 AU AU2002336876A patent/AU2002336876B2/en not_active Ceased
- 2002-11-06 JP JP2003542625A patent/JP2005508191A/ja active Pending
- 2002-11-06 MX MXPA04004278A patent/MXPA04004278A/es active IP Right Grant
- 2002-11-06 HU HU0401860A patent/HUP0401860A3/hu unknown
- 2002-11-06 ES ES02771969T patent/ES2224908T1/es active Pending
- 2002-11-06 EP EP02771969A patent/EP1453982A2/en not_active Withdrawn
- 2002-11-06 CA CA002464977A patent/CA2464977A1/en not_active Abandoned
- 2002-11-06 RU RU2004117153/13A patent/RU2363733C2/ru not_active IP Right Cessation
- 2002-11-06 CN CN028214900A patent/CN1578839B/zh not_active Expired - Fee Related
- 2002-11-06 US US10/494,742 patent/US8187859B2/en not_active Expired - Fee Related
-
2003
- 2003-06-10 ZA ZA2003/04529A patent/ZA200304529B/en unknown
-
2004
- 2004-03-12 CL CL200400517A patent/CL2004000517A1/es unknown
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861348A (zh) * | 2016-06-14 | 2016-08-17 | 江南大学 | 一株低产尿素的酿酒酵母及其在食品生产中的应用 |
| CN105861348B (zh) * | 2016-06-14 | 2019-04-23 | 江南大学 | 一株低产尿素的酿酒酵母及其在食品生产中的应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| HUP0401860A3 (en) | 2010-01-28 |
| ZA200304529B (en) | 2005-11-30 |
| AU2002336876B2 (en) | 2008-01-31 |
| CA2464977A1 (en) | 2003-05-15 |
| CL2004000517A1 (es) | 2005-04-15 |
| CN1578839B (zh) | 2012-02-08 |
| WO2003040379A3 (en) | 2003-09-04 |
| US8187859B2 (en) | 2012-05-29 |
| MXPA04004278A (es) | 2004-08-11 |
| EP1453982A2 (en) | 2004-09-08 |
| JP2005508191A (ja) | 2005-03-31 |
| DE02771969T1 (de) | 2005-02-10 |
| RU2004117153A (ru) | 2005-03-27 |
| HUP0401860A2 (hu) | 2004-12-28 |
| CN1578839A (zh) | 2005-02-09 |
| WO2003040379A2 (en) | 2003-05-15 |
| US20050069885A1 (en) | 2005-03-31 |
| RU2363733C2 (ru) | 2009-08-10 |
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